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1.
采用反相高效液相色谱法,选择苯酚为内标物,ZY1104型XWG-C18柱(4.6mm×250mm)为分析柱,甲醇-水-0.2mol/L硫酸铵(10.680.72,pH=7.7)为流动相,流速为1.0mL/min,检测波长为254nm,进样量20μL,测定片剂中盐酸莫索尼定的含量,按盐酸莫索尼定计算,理论板数为2544,样品峰与内标峰的分离度为3.9.线性范围为4~100mg/L(r=0.9998),最低检测量为10ng,平均回收率为99.82%,RSD=0.79%.用该法测定3批盐酸莫索尼定片剂的含量,结果分别为(96.8±1.9)%、(99.4±1.7)%和(99.3±1.6)%.本法快速、灵敏、准确,专属性高.  相似文献   

2.
盐酸吗啉胍片的HPLC测定   总被引:2,自引:0,他引:2  
采用高效液相色谱法,以WH-C18为固定相,扑尔敏为内标,甲醇-0.005mol/L醋酸铵(pH4.7)为流动相,测定盐酸吗啉胍片剂的含量。方法简便、快速准确。平均回收率99.74%,RSD0.60%。  相似文献   

3.
一阶导数光谱法测定呋麻滴鼻液含量   总被引:1,自引:0,他引:1  
吴履文 《中国药事》1995,9(6):365-365
本文采用导数光谱法不经分离直接测定呋麻滴鼻液含量。该法简便、快速、准确。盐酸麻黄素、呋喃西林的回收率分别为:100.68%(CV=0。89%);99.10%=(CV=0.88%)。  相似文献   

4.
目的:建立反相高效液相色谱法测定盐酸曲马朵片的含量。方法:以Spherisorb C18柱为分析,流动相为甲醇-0.05mol/L磷酸二氢钾溶液-三乙胺(40:60:0.1),用磷酸调节pH为3.5,检测波长为270nm。以对氨基苯甲酸为内标物,按峰面积内标法计算。结果:平均回收率为99.85%,RSD为0.1%(n=5),结论:本方法简便,准确。  相似文献   

5.
用高效液相色谱法测定盐酸伊达比星(Idarubicinhydrochloride),ODS柱,流动相为0.01mol/L十二烷基硫酸钠溶液:异丙醇(55:45),pH3.0,检测波长254nm,以β-萘磺酸作内标,盐酸伊达比星含量在0.005~0.500mg/ml内峰面积与浓度线性关系良好,注射剂的平均回收率为99.8%,重复进样相对标准差为0.4%,n=10,方法简便,准确。  相似文献   

6.
高效液相色谱法测定定喘止咳胶囊中三组份的含量   总被引:1,自引:0,他引:1  
用反相高效液相色谱法在ODS柱上以甲醇-0.1mol/L磷酸二氢钾液(pH3.0)-0.02mol/L庚烷磺酸钠液(58.5:35.5:6)为流动相,采用时间程序设定检测波长,选用醋酸洗必泰内标物对定喘止咳胶囊中氨茶碱,盐酸溴己新和扑尔敏进行了分离和含量测定,方法简便,准确,各组份的线性关系良好,平均回收率,氨茶碱99.8%,RSD1.27%,盐酸溴己新99.5%,RSD1.29%,扑尔敏96.4  相似文献   

7.
高效液相色谱法测定定喘止咳胶囊中三组分的含量   总被引:3,自引:0,他引:3  
用反相高效液相色谱法在ODS柱上以甲醇-0.1mol/L磷酸二氢钾液(pH3.0)-0.02mol/L庚烷磺酸钠液(58.5:35.5:6)为流动相,采用时间程序设定检测波长,选用醋酸洗必泰为内标物对定喘止咳胶囊中氨茶碱、盐酸溴己新和扑尔敏进行了分离和含量测定,方法简便,准确,各组份的线性关系良好,平均回收率:氨茶碱99.8%,RSD1.27%;盐酸溴己新99.5%,RSD1.29%;扑尔敏96.4%,RSD1.29%(n=6)。  相似文献   

8.
采用高效液相色谱法,以甲硝唑为内标,检测波长为240nm,以WH-C18为固定相,甲醇-0.02mol/L醋酸铵-0.5%硫酸铵(33:33.5:33.5)为流动相,测定安钠咖注射液中咖啡因和苯甲酸钠的含量;平均回收率(n=5)咖啡因为100.53%±0.27%,苯甲酸钠为99.87±0.64%。  相似文献   

9.
高效液相色谱法测定胃炎颗粒剂中盐酸普鲁卡因含量   总被引:1,自引:0,他引:1  
杨思利  肖驹 《首都医药》1999,6(6):29-30
本文应用高效液相色谱法直接测定胃炎颗粒剂中盐酸普鲁卡因的含量,以苯甲酸为内标,甲醇-醋酸盐缓冲液为流动相,选择285nm为测定波长。盐酸普鲁卡因在20-100μg/ml范围呈良好的性线关系,r=0.9998。盐酸普鲁卡因的平均回收率为99.96%,RSD=0.38%。  相似文献   

10.
HPLC法测定风湿松片中保泰松及氨基比林的含量   总被引:3,自引:1,他引:2  
采用反相HPLC法,以甲硝唑为内标,以C18为固定相,甲醇-水(70:30)为流动相,检测波长270nm,测定风湿松片中保泰松和氨基比林的含量。平均回收率保泰松为100.3%(RSD=0.98%)、氨基比林为99.5%(RSD=0.69%)。  相似文献   

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Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.
Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.
  相似文献   

14.
This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine.  相似文献   

15.
In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins.  相似文献   

16.
Zusammenfassung Mittels Gaschromatographie und Dünschichtchromatographie wiesen die Autoren 11 Substanzen nach, welche durch Injektion oder nach Verabreichung per os in die Kniegelenksynovialflüssigkeit eindrangen. In ihrer Aufstellung konnten sie eine direkte Beziehung zwischen Struktur sowie chemischphysikalischen Eigenschaften der Substanz und ihrer Fähigkeit, aus dem Blut in die Kniegelenksynovialflüssigkeit einzudringen, nicht nachweisen, außer der Tatsache, daß Substanzen mit starker Affinität zu Eiweißstoffen erst in höheren Dosen nachweisbar waren.  相似文献   

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Abstract

The uptake of metals from food and water sources by insects is thought to be additive. For a given metal, the proportions taken up from water and food will depend both on the bioavailable concentration of the metal associated with each source and the mechanism and rate by which the metal enters the insect. Attempts to correlate insect trace metal concentrations with the trophic level of insects should be made with a knowledge of the feeding relationships of the individual taxa concerned. Pathways for the uptake of essential metals, such as copper and zinc, exist at the cellular level, and other nonessential metals, such as cadmium, also appear to enter via these routes. Within cells, trace metals can be bound to proteins or stored in granules. The internal distribution of metals among body tissues is very heterogeneous, and distribution patterns tend to be both metal and taxon specific. Trace metals associated with insects can be both bound on the surface of their chitinous exoskeleton and incorporated into body tissues. The quantities of trace meals accumulated by an individual reflect the net balance between the rate of metal influx from both dissolved and particulate sources and the rate of metal efflux from the organism. The toxicity of metals has been demonstrated at all levels of biological organization: cell, tissue, individual, population, and community. Much of the literature pertaining to the toxic effects of metals on aquatic insects is based on laboratory observations and, as such, it is difficult to extrapolate the data to insects in nature. The few experimental studies in nature suggest that trace metal contaminants can affect both the distribution and the abundance of aquatic insects. Insects have a largely unexploited potential as biomonitors of metal contamination in nature. A better understanding of the physico-chemical and biological mechanisms mediating trace metal bioavailability and exchange will facilitate the development of general predictive models relating trace metal concentrations in insects to those in their environment. Such models will facilitate the use of insects as contaminant biomonitors.  相似文献   

20.
We report herein the condensation of 4,7-dichloroquinoline (1) with tryptamine (2) and D-tryptophan methyl ester (3) . Hydrolysis of the methyl ester adduct (5) yielded the free acid (6) . The compounds were evaluated in vitro for activity against four different species of Leishmania promastigote forms and for cytotoxic activity against Kb and Vero cells. Compound (5) showed good activity against the Leishmania species tested, while all three compounds displayed moderate activity in both Kb and Vero cells.  相似文献   

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