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1.
Echinacoside的中译名较早称海胆甙(《英汉医学词汇》,1979年)或海胆苷(《英汉化学化工词汇》第3版,1984年)。以后为多种文献辞书沿用,如1987年出版的《英汉科技辞天》和《英汉科技词库》,直至现在仍有人译为海胆苷。本人认为该中译名值得商榷。该化合物系Stoll等人于1950年首次从菊科紫松果菊属植物狭叶松果菊(或狭叶紫锥菊)Echi- 相似文献
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目的通过观察SNC6基因在大肠癌中的表达,结合临床病理及随访资料探讨其与生物学行为之间的关系。方法随机抽取经过山西省肿瘤医院1991—1992年诊治,有较完整的临床病理及随访资料的大肠癌病例92例,对每例病例的蜡块重新连续切片。其中苏木素-伊红(HE)切片按照最新的临床和组织学分类标准重新复习。其余切片采用免疫组织化学SP方法,进行单克隆抗体SNC6基因标记。用SPSS 13.0统计软件进行统计分析。使用χ2检验,Kaplan-Meier生存分析。结果大肠癌SNC6基因表达与患者年龄、性别、肿瘤部位、组织学类型、淋巴结转移以及肿瘤的临床分期均无明显相关性(P>0.05)。Kaplan-Meier生存分析显示:随着SNC6的表达增强,患者的生存时间明显延长,生存率明显提高。结论SNC6的表达与肿瘤的预后有关,是判断肿瘤预后的重要指标。SNC6可以作为判断大肠癌预后的一个独立指标。 相似文献
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紫锥菊属Echinacea植物根中海胆苷可用HPLC法测定,但该法纯化步骤费时且需复杂的仪器和多人操作等。作者采用中红外(IR)与金刚石衰减全反射(ATR)联用技术(可直接分析植物材料而无需任何纯化步骤)和近红外(NIR)光谱法,在约1 min内非破坏性分析紫锥菊E.purpurea(L.)Moench和狭叶紫锥菊E.angustifolia DC.根中海 相似文献
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目的 对市售海胆药材进行商品调查,并研究其热稳定性。方法 在国内沿海3市实地调查并收集海胆样品;用热重法(TG)及差示扫描量热法(DSC)对7个品种的海胆进行分析。结果 海胆药材市售商品流通量小,多属陈货,其中马粪海胆和海刺猬为主流品种,虾夷马粪海胆为日本引进品种。海胆的TG曲线基本一致,均有2个失重阶段,第一阶段为27~529℃,质量损失为2.38 %~5.06 %;第二阶段在612~805℃,质量损失为38.89 %~40.41 %;DSC曲线中,海胆样品在27 ℃至600 ℃区间均具1个较强吸热尖峰、1个吸热宽峰和2个放热峰,其中细雕刻肋海胆、虾夷马粪海胆的两个放热峰极弱。结论 7个品种市售海胆药材的热稳定性较好,并可通过热分析中的特征峰数量、位置、峰形和峰值大小等鉴别几种海胆。 相似文献
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海胆化学和药理学研究概况 总被引:5,自引:2,他引:3
海胆纲(Echinoidea)属棘皮动物门(Echinodermata)的游在亚门(Eleutherozoa),有球海胆科(Stronglocentrotidae)、毒棘海胆科(Toxopneustidae)、长海胆科(Echi-nometridae)等科。海胆有多种药用价值,据 相似文献
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方格星虫多糖对运动小鼠抗疲劳作用实验研究 总被引:2,自引:0,他引:2
目的探讨方格星虫多糖对小鼠的抗疲劳作用。方法将小鼠随机分组,采用小鼠负重游泳实验,以方格星虫多糖0.2,0.4,0.6g.kg-1灌胃给药,测定各组游泳时间,并对游泳后小鼠的血清尿素氮、乳酸脱氢酶、肝糖原、肌糖原、超氧化物歧化酶、丙二醛等指标进行检测。结果与对照组相比,星虫多糖各剂量组明显延长了小鼠的游泳时间。相应剂量给药小鼠血清尿素氮有明显减低,乳酸脱氢酶显著升高,肝糖原、肌糖原也呈上升趋势,低剂量用药能显著提高SOD活性和降低MDA含量。结论星虫多糖对小鼠有明显抗疲劳效果。 相似文献
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Immunomodulatory activity of polysaccharides isolated from Strongylocentrotus nudus eggs 总被引:1,自引:0,他引:1
Our previous work showed that SEP, a novel glucan isolated from the eggs of sea urchins, Strongylocentrotus nudus, had remarkable anti-tumor activity. To elucidate the mechanism of the anti-tumor activity, the immunomodulatory activity of SEP was investigated. The in vivo experiment results showed that SEP remarkably enhanced spleen and thymus index in S180-bearing mice, and also stimulated ConA-induced splenocyte proliferation. Immunomodulatory activity assay in vitro indicated SEP could significantly enhance the mouse splenocyte proliferation in a dose-dependent manner. According to comitogenic activity tests, SEP showed significant comitogenic activities and adjuvant properties. We also demonstrated that SEP had a unique mode of immunostimulation with regard to its cell-type specificity. In other words, SEP markedly stimulated B and T cell proliferation, however the influence on B cells was greatly weaker than that on T cells. IL-2, TNF-alpha, and IFN-gamma mRNA expression was upregulated after the mouse splenocytes were treated by SEP, indicating that Th1 cell was the primary cellular target affected by SEP on T lymphocyte. SEP enhanced production of nitric oxide (NO), upregulated mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages in a dose-dependent manner. In addition, SEP did not show direct toxicity to tumor cells. Consequently, the anti-tumor effect of SEP was related to stimulating host immunity/enhancing the immune system functions, which may mainly result from SEP activating lymphocytes and macrophages and stimulating secretion of some cytokines. 相似文献
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海胆黄多糖的分离、纯化及免疫活性测定 总被引:4,自引:1,他引:4
目的从光棘球海胆中分离纯化海胆黄多糖(polysaccharidefromtheeggsofStrongylocentrotusnu-dus,简称SEP),确定其纯度和分子量,并现察其免疫活性。方法海胆黄先经丙酮脱脂,根据正交实验和活性分析确定最佳热水提取条件,然后热水提取、去蛋白、醇沉得海胆黄粗多糖。粗多糖经超滤、DEAESepharoseFastFlow及SephacrylS-400柱层析纯化得多糖精品SEP。经高效液相色谱、聚丙烯酰胺凝胶电泳及纸层析鉴定其纯度。高效凝胶渗透色谱法(HPGPC)测定其分子量。体外脾淋巴细胞增殖实验测定其免疫活性。结果从海胆黄中分离纯化得到的均一多糖组分SEP,经检测其分子量为1950KD左右。脾淋巴细胞增殖实验表明SEP可显著促进脾淋巴细胞的增殖。结论从海胆黄中分离纯化得到的均一多糖组分SEP具有较强的体外免疫活性。 相似文献
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Interactions between the dopaminergic system and opioids have not been adequately clarified. The present study was designed to investigate the effects of micro-opioid (morphine), delta-opioid (SNC80) and kappa-opioid (U50 488H) receptor agonists on dopamine receptor agonist-induced climbing behavior in mice. Apomorphine (dopamine-receptor agonist) increased stereotyped climbing behavior, unlike methamphetamine, morphine, U-50 488H and (+/-)7-hydroxy-N,N-di-n-propyl-2-aminotetralin hydrobromide (D2-like receptor agonist). Furthermore, SKF81297 (D1 receptor agonist) and SNC80 caused climbing behavior. In addition, while morphine (20 mg/kg), but not U50 488H or SNC80, significantly attenuated high-dose apomorphine (2.0 mg/kg)-induced climbing behavior, it significantly potentiated low-dose apomorphine (0.5 mg/kg)-induced climbing behavior. These results suggest that morphine may have dual effects on the behavioral effects induced by apomorphine. Furthermore, we interestingly showed that the combination of apomorphine or SKF81297 and SNC80 enhanced frequent nonstereotypic climbing behavior, suggesting that delta/D1 interactions may play a prominent role in the expression of certain types of behavior in mice. Thus, micro-opioid, delta-opioid and kappa-opioid receptor agonists induce possible differential effects on the dopaminergic system in mice. 相似文献
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The effects of dopamine receptor agonists and antagonists on hyperlocomotion in mice induced by the nonpeptide delta-opioid receptor agonist (+)-4-[(aR)-a-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide) (SNC80) were investigated. SNC80 significantly increased locomotion (maximally at 2 mg/kg). In antagonism tests, naltrindole and naltriben completely attenuated this SNC80-induced hyperlocomotion, which suggests that SNC80-induced hyperlocomotion may be mainly mediated through delta-opioid receptors. Although haloperidol (dopamine D2-receptor antagonist) did not affect SNC80-induced hyperactivity, it inhibited morphine-induced hyperlocomotion. In combination tests, SNC80, at a dose that did not affect spontaneous activity, significantly potentiated hyperlocomotion induced by methamphetamine and the dopamine D1-receptor agonist 6-chloro-7,8-dihydroxy-1-phenyl-2,3,4,5-tetra-hydro-1H-3-benzazepin hydrobromide (SKF81297), whereas the combination of SNC80 and the D2-like receptor agonist 7-OH-N,N-di-n-propyl-2-aminotetralin did not affect locomotor activity. An earlier study demonstrated that the combination of the D1-receptor agonist SKF81297 and the D2-like receptor agonist 7-OH-N,N-di-n-propyl-2-aminotetralin synergistically induced hyperactivity in mice. Therefore, the present findings suggest that stimulation of either D2-like receptors or delta-opioid receptors can enhance the hyperlocomotion induced by stimulation of D1 receptors by methamphetamine and SKF81297, and the mechanism that underlies the hyperactivity caused by SNC80 may be different from that which underlies the effects of morphine. 相似文献
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通过FITC荧光标记海胆黄多糖SEP,研究大鼠尾静脉注射给药SEP的血浆代谢。结果表明,荧光标记的海胆黄多糖SEP-Tyr-FITC纯度可达99%。与空白对照组及SEP组相比,SEP-Tyr-FITC组小鼠脾细胞增殖无显著差异。其在大鼠体内的血浆清除半衰期为0.24 h,消除速率常数(Ke)为2.867,给药后的最大血药浓度(cmax)为860μg/mL,消除半衰期(t1/2)为0.24 h,药-时曲线下面积(AUCt)为366.4 h·μg/mL。所用方法的定量下限为0.02 mg/mL,标准曲线线性范围为0.02~1.0 mg/mL。最低定量限(LLOQ)、低、中、高4个浓度批内变异系数和批间变异系数均小于15%,相对回收率均在85%~115%之间。同时,各浓度SEP血浆样品处理前或处理后室温放置6 h、-20℃冷冻融解1次稳定性良好,相对标准偏差均小于10%。 相似文献
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不同产地裸体方格星虫脂肪酸类成分资源化学评价研究 总被引:1,自引:1,他引:0
目的 对裸体方格星虫样品中脂肪酸类成分进行比较研究。方法 采用气相色谱-质谱联用技术(GC-MS)对12个裸体方格星虫样品中的26种脂肪酸进行了含量测定,并进一步对各样品中的各成分的含量进行了主成分分析。结果 对裸体方格星虫样品的测定结果显示各样品26种脂肪酸类成分总含量范围为198.20?578.05 ?g/g,总含量最高的样品是海南三亚的裸体方格星虫体腔液样品(S12);各产地样品中脂肪酸类成分总含量均呈现体腔液部位高于外壁 内脏部位。测定样品的26种脂肪酸中,不饱和脂肪酸总含量高于饱和脂肪酸总含量;多不饱和脂肪酸中,亚油酸和?-亚麻酸含量最高,分别占总含量的1.54%?5.65%和1.61%?4.94%;单不饱和脂肪酸中顺芥子酸含量最高,分别占各样品总含量的14.66%?46.41%;基于26种脂肪酸的含量测定结果对12个裸体方格星虫样品的PCA分析结果显示不同产地裸体方格星虫的外壁 内脏样品相互接近,体腔液样品差别较大。结论 裸体方格星虫中脂肪酸不仅种类多样、含量丰富,而且具有多种重要活性作用的不饱和脂肪酸比例合理。本研究中对脂肪酸类成分的研究结果将对进一步开发利用裸体方格星虫提供一定的理论基础。 相似文献
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目的探讨囊泡单胺转运体(VMAT2)在脑组织中的分布特征与帕金森病(PD)的关系。方法使用利血平、神经毒素1-甲基-4-苯基-四氢吡啶(MPTP)及联合利血平和MPTP分别造成C57BL小鼠的PD模型和分别收集不同胎龄自然流产的新鲜胎儿,应用免疫组织化学方法和Western blot观察VMAT2和酪胺酸羟化酶(TH)在黑质致密部、腹侧被盖和蓝斑分布的变化。结果免疫组织化学分析及Western blot显示,PD小鼠较对照组小鼠VMAT2和TH阳性神经元细胞数目在黑质致密部明显减少,腹侧被盖和蓝斑中的阳性神经元细胞数无明显变化;同时发现在小鼠正常对照组和人胎脑中VMAT2在黑质致密部中的分布既少于腹侧被盖也少于蓝斑。结论这种对多巴胺神经元具有保护作用的VMAT2在黑质致密部中的分布少于腹侧被盖和蓝斑,黑质致密部保护作用薄弱是PD黑质选择性受损的重要原因。 相似文献
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Akiyoshi Saitoh Hiromu Tominaga Yasuhiro Ogawa Yoko Irukayama-Tomobe Mitsuhiko Yamada Masashi Yanagisawa Hiroshi Nagase 《Pharmacological reports : PR》2018,70(2):350-354
Background
We previously reported that the novel selective delta opioid receptor (DOP) agonist KNT-127 did not cause convulsions in mice, whereas the prototype DOP agonist SNC80 did. Previous studies have reported that SNC80 caused electroencephalographic (EEG) disturbances in rodents. However, whether KNT-127 affects EEG responses is unknown. Therefore, the present study aimed to compare the effect of KNT-127 on EEG responses with that of SNC80 in mice.Methods
For behavioral experiments, male C57BL6/J mice were injected intraperitoneally with either KNT-127 (30?mg/kg) or SNC80 (30?mg/kg) and monitored for convulsions and subsequent catalepsy-like behavior for 10?min immediately after drug treatment. For EEG recording experiments, EEG electrodes were implanted into the right hemisphere. EEG signals exceeding twice the baseline amplitude were defined as seizure spikes.Results
KNT-127 did not induce convulsive or catalepsy-like behaviors in mice and did not result in seizure spikes, while significantly higher EEG power density was observed at 2?Hz. In contrast, SNC80 administration resulted in convulsive behaviors, seizure spikes, and significantly higher EEG power density between 2 and 10?Hz in mice.Conclusions
In this study, we clearly demonstrated that KNT-127 administration induces neither convulsive effects nor seizure spikes in mice. We propose that KNT-127 should be considered a candidate compound for the development of improved DOP-based psychotropic drug that lack the convulsive properties. 相似文献20.
目的 研究方格星虫体壁肌肉和体腔液中的矿物元素组成及其与沉积物生境之间的相关性,旨在为方格星虫体内矿物元素开发利用和沉积物内元素的生物富集特征分析提供依据。 方法 在无扰动养殖区(N)、高强度采挖区(H)、中等强度采挖区(R)收集方格星虫和沉积物样品,应用电感耦合等离子体质谱法测定样品中的矿物元素。采用Pearson相关性分析和生物富集系数探讨方格星虫体壁元素与环境沉积物的关系。结果1)方格星虫肌肉中微量元素含量表现为钾>钠>镁>钙>铁>锌>铜>锶>砷>硒等,体腔液中的微量元素含量表现为钠>钾>镁>钙>铁>锶>锰>硒>砷>钒>钼>铅>镉>钴。Pearson相关分析发现,方格星虫肌肉钒元素含量和体腔液中的镉、钙元素均与沉积物具有显著正相关关系。2)方格星虫体壁肌肉中生物富集系数较高的矿物元素包括钾、镉、铜、硒、钼、锡和砷等,体腔液中生物富集系数较高的矿物元素包括钾、镉、钙、钠、锶等。不同处理组方格星虫体壁、体腔液中的矿物元素富集系数均表现出相近的变化趋势。3)高强度采挖组(H组)方格星虫体壁肌肉中的钾元素生物富集系数显著高于无扰动养殖组(N组)和中等强度采挖组(R组),而H组的镉、铜、钼生物富集系数显著低于N和R组(P<0.05)。高强度采挖组(H组)方格星虫体腔液中的钾、锶元素生物富集系数显著高于无扰动养殖组(N组)和中等强度采挖组(R组),而H组的镉、铜元素生物富集系数显著低于N组和R组(P<0.05),不同采挖方式可能影响了相关元素的分布或结合状态。结论 方格星虫体壁和体腔液矿物元素组成丰富,具有重要的开发利用价值。方格星虫对于不同元素的生物富集能力不同,部分元素含量受环境沉积物的影响。 相似文献