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1.
四氯对苯醌分光光度法测定环丙氟哌酸   总被引:3,自引:2,他引:1  
本文研究了在硼砂介质中,环丙氟哌酸与四氯对苯醌生成稳定的1:l络合物,表观摩尔吸收系数ε376=1.27×104L·mol-1·cm-1,基于这一反应,本方法测定药物制剂含量与文献方法一致,回收率为99.49%~100.2%,相对标准偏差为1.39%。  相似文献   

2.
氯氮平的电荷转移光度法测定   总被引:3,自引:0,他引:3  
以氯氮平作为电荷给体,7,7,8,8四氰基对二次甲基苯醌(TCNQ)作为电子受体,用分光光度法研究了它们之间形成电荷转移(CT)络合物的条件。结果表明:在丙酮介质中,二者于45℃水浴中恒温1h即可形成1∶1的络合物,其最大吸收波长为743nm,0~175μg/mL范围内呈线性关系,表观摩尔吸光系数ε=160×104L·mol-1·cm-1。方法的相对标准偏差小于1%(n=8)。对形成CT络合物的机理进行了探讨。应用拟定的方法对氯氮平片进行了含量测定,结果与标准方法一致,回收率为99%。  相似文献   

3.
高效液相色谱法测定复方健疗霜中三组分含量   总被引:8,自引:0,他引:8  
目的:测定复方健疗霜中曲安奈德、盐酸苯海拉明和硝酸咪康唑的含量。方法:高效液相色谱法,在ShimpackCLC-phenyl柱上,以甲醇-水相(三乙胺2mL,加水至300mL,磷酸调pH至76)为流动相,检测波长:230nm。结果:本文可同时测定三组分的含量。曲安奈德0020~0080g·L-1,盐酸苯海拉明0080~0320g·L-1,硝酸咪康唑0160~0640g·L-1范围内,峰面积与其浓度呈良好线性关系;平均回收率依次为1004%,RSD=102%;1006%;RSD=091%;1005%,RSD=060%。结论:方法简便、快速、准确,可作为样品的检测方法。  相似文献   

4.
高效液相色谱法测定胃利胶囊中橙皮苷的含量   总被引:10,自引:0,他引:10  
目的:建立胃利胶囊中橙皮苷含量测定方法。方法:用高效液相色谱法测定。选用YWG-C18分析柱(46mm×250mm,10μm),流动相:乙腈-水-甲醇-磷酸(20∶796∶04∶001),检测波长:283nm,流速:15mL·min-1,柱温:室温。结果:本法简便、灵敏、准确。线性范围:02~15μg(r=09993)。平均回收率:9939%,RSD=35%。结论:建立的定量方法可用于胃利胶囊的质量控制标准。  相似文献   

5.
高效液相色谱法测定八珍丸中芍药苷的含量   总被引:32,自引:0,他引:32  
目的:应用高效液相色谱法对八珍丸中芍药苷进行含量测定。方法:选用KromasilC18分析柱(250mm×46mm,5μm),甲醇-水(35∶65)为流动相,检测波长为230nm,流速08mL·min-1。结果:线性范围:16~96μg(r=09999),平均回收率9776%,RSD为10%。结论:本法简便、灵敏、准确。  相似文献   

6.
高效液相色谱法测定仙茅中仙茅苷的含量   总被引:7,自引:1,他引:6  
目的:建立反相高效液相色谱法测定仙茅中仙茅苷的含量。方法:样品经甲醇提取,色谱柱为AltimaC18(5μm,46mm×250mm),柱温40℃,甲醇-异丙醇-水(20∶5∶75)为流动相,流速10mL·min-1,检测波长283nm。结果:仙茅苷在025~259μg范围内呈良好的线性关系,平均回收率为9967%,RSD为26%(n=4)。结论:本方法简便、可靠,重现性好。  相似文献   

7.
舒必利及其片剂的比色测定法   总被引:3,自引:1,他引:2  
目的:测定舒必利制剂中舒必利的含量,增加质量控制手段。方法:利用舒必利与7,7,8,8四氰基对二次甲基苯醌(TCNQ)形成1∶1荷移络合物,应用比色法测定制剂中舒必利含量。结果:药物浓度在2~26μg·mL-1范围内呈线性关系,样品回收率为991%~993%(n=3)。结论:方法简便、灵敏、准确,可做为该药制剂的含量测定方法。  相似文献   

8.
高效液相色谱法测定洋金花药材中东莨菪碱的含量   总被引:14,自引:0,他引:14  
目的:建立测定洋金花药材中东莨菪碱含量的高效液相色谱法。方法:采用Hypersil(5μm,46mm×200mm)色谱柱,以甲醇-水(53∶47,水中含20mmol·L-1醋酸钠,002%三乙胺,03%四氢呋喃,pH为686)为流动相,紫外检测波长215nm,外标法定量。结果:东莨菪碱浓度在2~666μg·mL-1范围内呈线性关系,方法回收率为9909%(n=6),RSD为074%。结论:此法灵敏度高,重现性好。  相似文献   

9.
高效液相色谱法检测血清中的高三尖杉酯碱   总被引:4,自引:0,他引:4  
目的:利用高效液相色谱法测定血清中的高三尖杉酯碱的含量。方法:以内标法为基础,取1mL血清标本加入内标三尖杉酯碱,经二氯甲烷2次重复提取,减压蒸干,残渣用流动相溶解,进样。色谱条件:以C18反相柱为分析柱,流动相组成为01mol·L-1甲酸铵-甲醇(2∶1),流速为10mL·min-1,在紫外检测器波长290nm处进行检测。结果:方法学检查:最低检测限2ng·mL-1,在4~1500ng·mL-1范围内呈线性,回收率913%(n=8),批内和批间的相对标准偏差分别为12%(n=8)和36%(n=4)。结论:对临床化疗常与之配伍使用的3种药物,进行干扰实验,未发现干扰峰。  相似文献   

10.
氟哌酸胶囊溶出度测定方法的试验   总被引:1,自引:0,他引:1  
目的:探讨紫外分光光度法测定氟哌酸胶囊含量的条件、方法和氟哌酸胶囊溶出度的测定。方法:用紫外分光光度法测定氟哌酸胶囊含量及转篮法测定其溶出度。结果:在273nm 波长处有最大吸收(5μg/ml,04 % 氢氧化钠溶液) ,其吸收系数平均值( E1 %1 cm ) 为1095 , 线性关系良好( r =09998) 。20min 溶出率( % ) 均在80 % 。结论:氟哌酸胶囊含量测定可采用紫外分光光度法,简便易行,快速准确。同时可用于氟哌酸胶囊溶出度测定。  相似文献   

11.
The therapeutic efficacy of four quinolones, i.e. ciprofloxacin, ofloxacin, norfloxacin and pipemidic acid, was investigated in experimental infections in mice caused by pipemidic acid-susceptible and -resistant E. coli. For intraperitoneal infections caused by E. coli strain 444 and 23, the efficacy of ciprofloxacin, ofloxacin and norfloxacin was superior to that of pipemidic acid. Furthermore, ciprofloxacin and ofloxacin had higher activity than norfloxacin and pipemidic acid in urinary tract and uterine infections. Serum and uterus levels of ciprofloxacin and ofloxacin in normal mice were higher and more durable than those of norfloxacin.  相似文献   

12.
Summary The pharmacokinetics of caffeine, including formation of its major metabolite paraxanthine in plasma, has been investigated in 12 healthy males (age 20–40 years) alone and during co-administration of the 4-quinolones ofloxacin, norfloxacin, pipemidic acid, ciprofloxacin, and enoxacin; ciprofloxacin and enoxacin were given in 3 different dose levels.The naphthyridine derivative enoxacin and the pyrido-pyrimidine derivative pipemidic acid had caused marked inhibition of caffeine and paraxanthine metabolism, whereas the genuine quinolone derivatives norfloxacin and ciprofloxacin had little effect, and the pyrido-benzoxacine derivative ofloxacin had no detectable effect.The different molecular and spatial structures of the compounds appear to be responsible for the differences in inhibitory potency.  相似文献   

13.
The in-vitro inhibition of several metabolic pathways has been studied in 3-methylcholanthrene-treated rats. The specificity of the 7-ethoxyresorufin O-de-ethylase reaction has been determined in the presence and absence of ciprofloxacin, enoxacin, norfloxacin, ofloxacin, nalidixic acid, oxolinic acid and pipemidic acid. For the caffeine N3-demethylation reaction, enoxacin and pipemidic acid were used. Enoxacin (IC50 = 105 microM, Ki = 65 microM) and pipemidic acid (IC50 = 115 microM, Ki = 160 microM) significantly inhibited 7-ethoxyresorufin O-de-ethylase reaction and caffeine N3-demethylation (IC50 = 60 microM for enoxacin and IC50 = 185 microM for pipemidic acid) by a competitive mechanism. Other quinolones had lower or no (ofloxacin) inhibitory capacity. The order of inhibitory activity observed is in agreement with results obtained previously from in-vivo studies in man. No activity was detected towards ethylmorphine N-demethylation.  相似文献   

14.
Capillary electrophoresis (CE) was applied to the study of 10 quinolones of first and second generation--nalidixic acid, oxolinic acid, pipemidic acid, cinoxacin, norfloxacin, ciprofloxacin, ofloxacin, pefloxacin, fleroxacin, and flumequine. Separation was performed on a fused silica capillary (75 microm-60 cm) using a phosphate buffer (pH 7.0, 125 mM). Detection was at 214 nm. Only norfloxacin and ciprofloxacin cannot be separated in this way. Because of the specificity of the method, the identification of the individual quinolones by their migration time was possible. The same system has been applied for the quantitative determination of quinolones in tablets and capsules. Excipients do not adversely affect the results. Some parameters (linearity, precision, accuracy) were validated. Especially the possibility of simultaneous quantification and identification of the active ingredient in the finished product is very attractive.  相似文献   

15.
A spectrophotometric method was described for the determination of the antibacterial quinolone derivatives, ciprofloxacin, enrofloxacin and pefloxacin through charge transfer complex formation with three different acceptors. Chloranilic acid (CL) was utilized for their determination, forming charge transfer complex with lambdamax 520 nm. The proposed method was applied for determination of Ciprocin tablets, Enroxil oral solution, Peflacin ampoules and Peflacin tablets, with mean percentage accuracies, 99.58+/-1.25,99.94+/-0.96,100.91+/-1.59 and 99.86+/-1.003. Also, tetracyanoethylene (TCNE) was utilized in the determination of the concerned compounds forming charge transfer complexes with maximum absorbances at lambdamax 335 nm for ciprofloxacin and at lambdamax 290 nm for both enrofloxacin and pefloxacin. The procedure was applied for determination of Ciprocin tablets, Enroxil 10% oral solution, Peflacine tablets and Peflacine ampoules with mean percentage accuracies 99.40+/-1.27,99.95+/-0.90,98.98+/-1.565 and 99.88+/-0.998, respectively. Also, 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) was utilized for determination of pefloxacin forming charge transfer complex with maximum absorbance at lambdamax 460 nm. The procedure was applied for determination of peflacine tablets and peflacine ampoules with mean percentage accuracies 100.40+/-0.76 and 99.91+/-0.623, respectively. Statistical analysis of the obtained results showed no significant difference between the proposed method and other official and reported methods as evident from the t-test and variance ratio.  相似文献   

16.
目的:建立乳酸环丙沙星乳膏的含量测定方法。方法:利用乳酸环丙沙星与2.4-二硝基酸产生反应,可形成电荷转移络合物,以分光光度法测定。结果:荷移反应后,乳酸环丙沙星最大吸收波长为398nm,在此波长处基质无干扰,吸收度与浓度间呈良好的线性关系,平均回收率为98.6%,RSD=0.8%(n=4)。结论:用荷移分光光度法可排除基质干扰,准确测定乳酸环丙沙星乳膏的含量。  相似文献   

17.
Some (1-pyrryl)methyl derivatives of 1-ethyl-1,4-dihydro-4-oxoquinoline-3-carboxylic acid were synthetized by the standard procedure involving Gould-Jacobs and Lappin reactions. The above derivatives were tested microbiologically as nalidixic acid analogs and compared with some clinically useful 4-oxopyridine-3-carboxylic acids (nalidixic acid, pipemidic acid, norfloxacin, enoxacin and ciprofloxacin). Their antibacterial activities were very weak.  相似文献   

18.
A simple and sensitive method is described for the determination of fluoroquinolones by HPLC on a C-18 column using fluorescence detection. Using a mobile phase of 25% (v/v) acetonitrile phosphate buffer (pH 2.0), adequate retention and separation among the solutes norfloxacin, amifloxacin, enoxacin, and pipemidic acid have been obtained using sodium lauryl sulphate as the pairing ion and tetrabutylammonium bromide as the counter ion. The chromatographic conditions selected have been used for the quantitation of norfloxacin, amifloxacin, and enoxacin in human plasma using pipemidic acid as the internal standard. A simple single-step protein precipitation procedure has been employed for pretreatment of plasma samples. The detection limits of the assay for enoxacin, amifloxacin, and norfloxacin are approximately 100, approximately 10, and approximately 20 ng/mL, respectively. The method has been employed for the determination of amifloxacin in plasma samples from a healthy volunteer following oral administration of a 400-mg amifloxacin capsule.  相似文献   

19.
Microspheres containing the mucoadhesive polymer chitosan hydrochloride, with matrix polymer Eudragit RS, pipemidic acid as a model drug and agglomeration preventing agent magnesium stearate were prepared by the solvent evaporation method. The amount of magnesium stearate was varied and the following methods were used for microsphere evaluation: sieve analysis, drug content and dissolution determination, scanning electron microscopy, xray diffractometry, DSC and FTIR spectroscopy. The results showed that average particle size decreased with increasing amount of magnesium stearate used for microsphere preparation. This is probably a consequence of stabilization of the emulsion droplets with magnesium stearate. Higher pipemidic acid content in the microspheres was observed in larger particle size fractions and when higher amounts of magnesium stearate were used. It was also found that these two parameters significantly influenced the dissolution rate. The important reason for the differences in drug content in microspheres of different particle sizes is the diffusion of pipemidic acid from the acetone droplets in liquid paraffin during the preparation procedure. The physical state of pipemidic acid changed from crystalline to mostly amorphous with its incorporation in microspheres, as shown by x-ray diffractometry and differential  相似文献   

20.
环丙氟哌酸的药效学和毒理学研究   总被引:3,自引:1,他引:2  
本文研究了环丙氟哌酸的体外抗菌作用、体内保护作用、小鼠和大鼠一次给药的半数致死量和安全试验。结果表明,环丙氟哌酸对革兰氏阴性菌的MIC范围为0.005~0.19μg/ml,抗菌活性强于氟哌酸、氟啶酸和吡哌酸;对革兰氏阳性菌的 MIC为0.045~1.50μg/ml,其抗革兰氏阳性菌活性明显强于上述对照药。小鼠分别感染金葡球菌、大肠杆菌和绿脓杆菌后的半数有效量明显优于氟哌酸、氟啶酸和吡哌酸。小鼠一次给药的半数致死量,口服LD_(50)为2991.52mg/kg; 静脉为223.88mg/kg;肌肉和皮下的LD_(50)分别为831.08mg/kg和1133.42mg/kg. 大鼠口服LD_(50)>5000mg/kg,静脉LD_(50)>200mg/kg;肌肉和皮下分别为>1000mg/kg和>1200mg/kg。狗口服50mg/kg日服三次为期2日和口服100mg/kg日服一次为期7日,未见毒性反应和功能改变,也未见病理组织学变化。  相似文献   

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