首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 46 毫秒
1.
目的:对比研究超声法和连续回流法提取黄芪总黄酮的工艺。方法:以乙醇浓度、提取时间、固液比、提取温度为考察因素,黄芪总黄酮提取率为考察指标,采用正交试验优选最佳提取工艺条件。结果:要获得相同的提取率,超声法效率更高,其最佳提取工艺为乙醇浓度75%,超声提取时间20min,固液比1∶10,超声提取温度25℃,提取率为0.325%。结论:与连续回流法比较,采用超声法提取黄芪总黄酮具有快速、节省溶剂、提取的有效成分含量高等优点。  相似文献   

2.
目的:优选莲子心的提取工艺。方法:以莲心碱提取率为指标,以乙醇浓度、乙醇用量、提取时间、提取次数为考察因素,采用正交试验法优选最佳提取工艺。结果:最佳提取工艺为乙醇浓度80%,乙醇用量为4倍莲子心药材量,提取次数2次,每次1 h。结论:该工艺是在模拟生产条件下优选出的,对指导生产具有参考价值。  相似文献   

3.
目的探讨优选超声法提取香蕉皮的工艺。方法以香蕉皮多糖提取率为指标,研究超声功率、提取时间、料液比、提取温度等因素对提取率的影响,优选最佳提取工艺。结果香蕉皮多糖的最佳提取条件为超声波功率100 W,提取时间20 min,料液比1:40,提取温度50℃。在最佳提取条件下,香蕉皮粗多糖的得率为6.03%。结论优选的香蕉皮多糖提取工艺的提取率较高,工艺稳定可靠简单。  相似文献   

4.
目的优选芍黄通便合剂水提取工艺。方法以芍药苷提取率作为主要考察指标,采用L9(34)正交试验法选择最佳水提取工艺。结果芍黄通便合剂最佳水提取工艺为加8倍量水,煎煮2次,每次1.5 h。结论该工艺是在模拟生产条件下优选出的,其有效成分提取率高,重现性好,稳定可行,可为芍黄通便合剂大生产工艺提供参考。  相似文献   

5.
目的采用正交试验优化养正消积胶囊中醇提部分(人参、女贞子)超声逆流提取工艺。方法以熊果酸和齐墩果酸的总含量提取率及干膏收率两个考察指标进行综合评分,采用L9(34)正交实验设计优选出超声逆流提取的最佳工艺。结果最佳超声逆流提取工艺条件是:超声频率27 k Hz,料液比1:4,乙醇浓度80%,超声温度45℃。结论该法经验证稳定可靠,适用于大批量生产。  相似文献   

6.
正交法优选丹参提取工艺研究   总被引:4,自引:1,他引:3  
目的:优选丹参的提取工艺。方法以丹酚酸B及丹参酮ⅡA的提取率为考察指标,对丹参的提取工艺进行正交试验法,筛选出最佳提取工艺。结果优选出的丹参最佳提取工艺为:8倍量70%乙醇在70℃下加热回流提取90 min。结论优选得到的丹参提取工艺稳定可行。  相似文献   

7.
正交试验优选竹节参多糖提取工艺   总被引:1,自引:1,他引:0  
袁丁  赵海霞  胡远浪  何毓敏 《中国药房》2010,(31):2897-2899
目的:优选竹节参多糖的提取工艺。方法:采用正交试验法,以总多糖的提取率为考察指标,以溶媒用量、提取时间、提取次数为考察因素优选提取工艺。结果:最佳提取工艺为溶媒用量10倍,每次提取时间2.0h,提取3次。结论:优选的工艺提取率高、稳定、可行。  相似文献   

8.
目的:优选水飞蓟总黄酮提取最佳工艺条件。方法:以水飞蓟宾含量及总黄酮提取率为综合评价指标,在单因素试验基础上,通过正交试验考察混合溶剂的配比、料液比及提取时间3个因素对水飞蓟宾含量及总黄酮提取率的影响。结果:水飞蓟壳提取最佳工艺条件是乙酸乙酯与无水乙醇按1:2混合,提取时间为8 h,料液比1:6,提取方式为逆流提取。结论:该提取工艺操作简便合理,重复性良好,可用于大规模生产。  相似文献   

9.
正交试验优选槲皮素提取精制工艺   总被引:3,自引:1,他引:2  
王刚  兰鸿  常明泉  杨光义  曾南  叶方 《中国药师》2010,13(10):1413-1415
目的:优选从槐米中提取槲皮素的工艺。方法:采用正交试验法,以提取物中槲皮素的含量和槲皮素的提取率为指标,采用多指标综合分析方法优选最佳槲皮素提取、精制工艺。结果:从槐米中提取槲皮素的最佳工艺为:芦丁酸沉的pH为4,芦丁水解时加入的酸量1:80,水解时间2h。结论:该工艺可提高槐米中槲皮素的提取率和纯度。  相似文献   

10.
目的利用响应面法优选鹿药总黄酮的提取工艺条件。方法以乙醇体积分数、溶媒用量、提取时间为主要影响因素,以总黄酮提取率为评价指标,用响应面法选择提取工艺的最佳条件。结果响应面法筛选的最佳提取工艺为:82.6%乙醇,溶媒用量11.4倍量,提取时间1.91h,理论提取率为2.31%,实际测得值为2.26%。结论星点设计-响应面法优选鹿药中总黄酮提取工艺,方法简单,结果可靠。  相似文献   

11.
12.
13.
This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine.  相似文献   

14.
In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins.  相似文献   

15.
16.
Zusammenfassung Mittels Gaschromatographie und Dünschichtchromatographie wiesen die Autoren 11 Substanzen nach, welche durch Injektion oder nach Verabreichung per os in die Kniegelenksynovialflüssigkeit eindrangen. In ihrer Aufstellung konnten sie eine direkte Beziehung zwischen Struktur sowie chemischphysikalischen Eigenschaften der Substanz und ihrer Fähigkeit, aus dem Blut in die Kniegelenksynovialflüssigkeit einzudringen, nicht nachweisen, außer der Tatsache, daß Substanzen mit starker Affinität zu Eiweißstoffen erst in höheren Dosen nachweisbar waren.  相似文献   

17.
Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.
Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.
  相似文献   

18.
19.
This article assesses pain within the context of the dose response. A substantial number of studies indicate that the dose response for pain-related endpoints is commonly biphasic, being independent of the type of biological model employed, endpoint measured, or agent tested. The quantitative features of the dose response are also remarkably consistent regardless of the receptor pathway that mediates the nociceptive response, indicating a likely downstream message convergence. These findings have important implications for drug discovery, development, and clinical evaluation.  相似文献   

20.
The current USP National Formulary contains 65 Monographs for drug formulations containing neomycin. All 65 Monographs prescribe a bioassay for neomycin assay. This bioassay, based on cell culture, is labor intensive, has poor precision, and cannot be adapted for purity or identification. High-performance anion-exchange chromatography with integrated pulsed amperometric detection (HPAE-IPAD), a liquid chromatography technique, has been shown to be suitable for neomycin purity analysis and neomycin assay of an over-the-counter first aid cream (Hanko and Rohrer [17]). Here we propose that an HPAE-IPAD assay can replace the bioassay in the 65 neomycin-containing Monographs. We applied the HPAE-IPAD assay to four neomycin-containing drug products representing the four classes of formulations found in the 65 Monographs, liquid, solid, suspension, and cream. Each drug was analyzed with two chromatography systems, and on 3 separate days. For all products, HPAE-IPAD measurements were precise and accurate with respect to the label concentrations. There was also high accuracy for spike recovery of neomycin from the four drug products throughout 70–150% of the labeled concentration. These results suggest that an HPAE-IPAD assay would be an accurate assay for neomycin, and would be faster and more precise than the current bioassay.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号