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1.
目的 研究素心腊梅种子中的生物碱成分.方法 采用柱色谱和重结晶法分离纯化生物碱,并采用核磁共振技术鉴定其结构.结果 从素心腊梅种子中共分离得到5个生物碱单体成分,分别为d-夏腊梅碱(d-calycanthine,Ⅰ)、l-folicanthine(Ⅱ)、chimonamidine (Ⅲ)、calycanthidine (Ⅳ)和1-腊梅碱(chimonanthine,Ⅴ).结论 所有化合物均为首次从该植物中得到.  相似文献   

2.
目的 对小叶地不容(Stephania succifera H. S. Lo et Tsoong)块根的细胞毒活性成分进行研究。方法 采用多种柱色谱技术进行化合物分离纯化,根据波谱数据和理化性质鉴定化合物的结构;采用MTT法对化合物进行细胞毒活性测试。结果 从小叶地不容块根的乙酸乙酯萃取物中分离得到5个化合物,分别鉴定为7-oxodehydrocaaverine (1)、7-氧代克班宁(2)、去氢克班宁(3)、克班宁(4)、马兜铃内酰胺I (5)。细胞毒活性结果显示,化合物3、4对慢性髓原白血病细胞(K562)、人胃癌细胞(SGC-7901)和人肝癌细胞(SMMC-7721)的增殖显示出生长抑制活性。结论 首次从小叶地不容块根中分离得到化合物1和5,化合物1为首次从植物中分离得到的新天然产物。  相似文献   

3.
草连生物碱成分的研究   总被引:6,自引:1,他引:6  
目的研究草连的生物碱成分。方法采用硅胶柱色谱法,进行单体化合物的分离纯化,通过波谱学方法鉴定其结构。结果分离并鉴定出7个原小檗碱型生物碱,分别为小檗碱、药根碱、巴马汀、非洲防己碱、黄连碱、甲基黄连碱和groen-landicine。结论7种生物碱均首次从草连中分得。草连所含生物碱的种类与黄连属其他植物中的生物碱种类不完全相同,生物碱种类可作为黄连属植物分类及生药鉴定的依据之一。  相似文献   

4.
用高效液相色谱仪(HP-1050、1040)对新疆骆驼蓬植物种子总生物碱进行分离,得出骆驼蓬种子总生物碱色谱图,对Harmin生物碱峰进行纯度鉴定,并得出Harmin λmax为214.5nm,297.5nm,337.5nm其紫外光谱图与文献及Harmin。纯品相符。可确定骆驼蓬种子总生的碱中含有Harmin生物碱。  相似文献   

5.
百蕊草生物碱成分的分离与鉴定   总被引:2,自引:0,他引:2  
目的 研究百蕊草中的生物碱成分.方法 应用多种色谱手段进行分离纯化,通过IR、MS、HR-MS、NMR、2D-NMR技术鉴定化合物结构.结果 从百蕊草总生物碱部位分离得到3个化合物,分别鉴定为N-甲基金雀花碱(1)、白金雀儿碱(2)和槐果碱(3).结论 化合物N-甲基金雀花碱、白金雀儿碱、槐果碱均为首次从该植物中分离得到.  相似文献   

6.
目的研究交让木中的生物碱成分。方法样品用95%的乙醇进行提取,所得浸膏用酸-碱处理方法处理后得到总生物碱,之后经正相硅胶、Sephadex LH-20、反向硅胶(RP-18)、高效制备液相等多种色谱方法分离得到生物碱单体,根据其理化性质及波谱学数据鉴定其化学结构。结果分离得到4个虎皮楠生物碱,经鉴定分别为daphniyunnine B(1)、daphmacrodin A(2)、macropodumine B(3)、daphnicyclidin L(4)。结论化合物daphniyunnine B(1)为首次从该植物中分离得到。  相似文献   

7.
目的对来源于西沙群岛的海绵(Agelas sp.)共附生微生物Aspergillus alabamensis(18-xs-01-zp-3)进行菌种鉴定及次级代谢产物的研究。方法通过分子生物学方法鉴定菌株种属;综合运用硅胶柱层析、薄层色谱和半制备高效液相色谱(HPLC)等色谱学方法对次级代谢产物进行分离纯化,通过核磁共振(NMR)、质谱(MS)等现代波谱学方法并结合文献对所得化合物进行结构鉴定。结果从中分离得到7个单体化合物,分别是ardeemin型生物碱3个:5-N-acetyl-15bβ-hydroxyardeemin(1)、5-N-acetylardeemin(2)、5-N-acetyl-15b-didehydroardeemin(3);二酮哌嗪生物碱2个:3-benzylidene-3,4-dihydro-4-methyl-lH-l,4-benzodiazepine-2,5-dione(4)、benzodiazepinedione(5);吲哚类生物碱2个:luteoride E(6)、14-hydroxyterezine D(7)。结论从该菌株共分离得到7个生物碱类化合物,化合物1对H69AR具有抗肿瘤活性,化合物4为首次从曲霉属真菌中分离得到。  相似文献   

8.
Hong B  Li WJ  Zhao CJ 《药学学报》2012,47(6):764-768
为研究夹竹桃科植物萝芙木的化学成分及药理活性,通过硅胶、凝胶LH-20、反相开口柱等色谱方法进行分离纯化。根据化合物的理化性质和波谱数据鉴定化学结构,从氯仿层提取物中分离得到3个吲哚类生物碱和1个吖啶酮类生物碱,分别鉴定为萝芙碱B(1)、山德维辛碱(2)、萝尼生(3)和7-羟基-吖啶酮(4)。化合物1为新化合物,属于吲哚类生物碱。化合物4是吖啶酮类生物碱,为首次从萝芙木属植物中分离得到的化合物类型。本文对化合物4这一新类型的生物碱做了生物活性研究,以发掘萝芙木除降压以外其他的药理活性。  相似文献   

9.
目的探讨去氢骆驼蓬碱、骆驼蓬碱、骆驼蓬总碱及哈尔满碱(止咳药用植物)对DNA拓扑异构酶Ⅱ活性的抑制作用。方法从体外培养的Q3肝癌细胞中,提取分离DNA拓扑异构酶Ⅱ;以阿霉素为阳性对照,用琼脂糖凝胶电泳法检测药物对DNA拓扑异构酶Ⅱ的作用。结果骆驼蓬总碱、去氢骆驼蓬碱、骆驼蓬碱及哈尔满碱对DNA拓扑异构酶Ⅱ活性均有一定的抑制作用。结论对DNA拓扑异构酶Ⅱ活性的抑制作用是骆驼蓬总碱、去氢骆驼蓬碱、骆驼蓬碱等生物碱抗癌作用和细胞毒作用的机制之一。  相似文献   

10.
目的:研究黔产药用植物忽地笑中的化学成分。方法:采用多种色谱手段和重结晶等方法,分离纯化化合物;根据化合物的理化性质和波谱数据鉴定其结构。结果:从忽地笑鳞茎95%乙醇提取物的粗碱部位中分离得到7个单体化合物,均为生物碱,分别被鉴定为石蒜碱(lycorine,1),二氢石蒜碱(dihydrolycorine,2),加兰那敏(galanthamine,3),力可拉敏(lycoramine,4),那维定(narwedine,5),lycoraminone(6)和2-羟基-6-甲氧基香水仙灵(2-hydroxyl-6-O-methyloduline,7)。结论:化合物5、6和7均为首次从该植物中分离得到。  相似文献   

11.
Two new quaternary protoberberine alkaloids, namely corydayanine (1) and yanhusuine (2), were isolated from the tubers of Corydalis yanhusuo. On the basis of extensive chemical and spectroscopic evidences, especially 1D and 2D NMR and HRMS experiments, their structures were elucidated as 5,6-dihydro-3,9-dihydroxy-2,10-dimethoxy-13-methyl-dibenzo[a,g] quinolizinium alkaloid and 5,6-dihydro-12-hydroxy-2,3,9-trimethoxy-13-methyl-dibenzo[a,g] quinolizinium alkaloid, respectively.  相似文献   

12.
Five new C18-diterpenoid alkaloids, anthriscifoltines C-G (1–5), along with four known diterpenoid alkaloids anthriscifolcines C-F (6–9), were isolated from the extract of Delphinium anthriscifolium var. majus. Their structures were elucidated by extensive spectroscopic analyses (including 1D-, 2D-NMR, and HR-ESI-MS). Compounds 1–5 were also evaluated for their cytotoxic activity against MCF-7, HepG2, and H460 human cancer cell lines.  相似文献   

13.
《Pharmaceutical biology》2013,51(3):400-404
Context: Pseuduvaria rugosa (Blume) Merr. (Annonacaea) grows widely in the south and southeast regions of Thailand. Preliminary screening for biological activities revealed that crude hexane, ethyl acetate, and acetone extracts from mixtures of leaves and twigs of P. rugosa showed cytotoxicity.

Objective: Chemical constituents and their antiproliferative activity in K562, U937, and HL-60 human leukemic cell lines from P. rugosa were performed for the first time.

Materials and methods: The isolated compounds were obtained from chromatographic separation. The structures were established by spectroscopic techniques including IR, UV, NMR together with 2D NMR (HMBC, COSY, and NOE) and MS. The K562, U937, and HL-60 cell lines were treated with isolated aporphine alkaloids (0–100 µg/mL) and cell viability was measured with the MTT assay. Cell cycle analysis was performed using propidium iodide (PI) based staining methods.

Results: Two known aporphine alkaloids, 1,2,3-trimethoxy-5-oxonoraporphine (1) and ouregidione (2) were isolated. Treatment of the cells with compounds 1 and 2 at a concentration of 100 µg/mL for 72?h reduced the viability of K562, U937, and HL-60 cell lines to 63 and 64, 38 and 66, and 49 and 64%, respectively. In addition, compounds 1 and 2, at a concentration of 100 µg/mL, exposed to U937 and HL-60 cell lines showed cell cycle arrest. The U937 cell line treated with compounds 1 and 2 increased significantly the proportion of the cell in S phase, whereas the HL-60 cell line-induced G2/M and G1 phase, respectively.

Discussion and conclusion: The results showed that 1,2,3-trimethoxy-5-oxonoraporphine and ouregidione-induced cytotoxicity with HL-60, U937, and K562 cells where 1,2,3-trimethoxy-5-oxonoraporphine was more active than ouregidione.  相似文献   

14.
Six protoberberine alkaloids were isolated from the chloroform layer of the rhizome of Coptis japonica Makino (Ranunculaceae). The structures of the isolated compounds were determined to be 6-([1,3]dioxolo[4,5-g]isoquinoline-5-carbonyl)-2,3-dimethoxy-benzoic acid methyl ester (1), oxyberberine (2), 8-oxo-epiberberine (3), 8-oxocoptisine (4), berberine (5) and palmatine (6) by physicochemical and spectroscopic methods. The compound 3 (8-oxo-epiberberine) was first isolated from natural sources. The compounds were tested for cytotoxicity against five tumor cell lines in vitro by SRB method, and also tested for the MDR reversal activities. Compound 4 was of significant P-gp MDR inhibition activity with ED50 value 0.018 microg/mL in MES-SA/DX5 cell and 0.0005 microg/mL in HCT15 cell, respectively.  相似文献   

15.
Phytochemical investigation on the 70% ethanol extract of the leaves of Alstonia mairei resulted in the isolation of three new monoterpenoid indole alkaloids, alstomairines A–C (13), along with one known compound, alpneumine A (4). Structural elucidation of all the compounds was accomplished by spectral methods such as 1D and 2D NMR, IR, UV, and HRESIMS. The isolated compounds were tested in vitro for cytotoxic activities against four osteosarcoma cell lines. Consequently, alkaloids 2 and 3 exhibited cytotoxic activities for all tested tumor cell lines with IC50 values from 9.2 to 13.0 μM.  相似文献   

16.
目的研究小叶锦鸡儿中异黄酮及其苷类化合物的体外抗肿瘤细胞增殖作用。方法利用四唑盐比色法检测,观察小叶锦鸡儿中分离出的3种异黄酮及其苷类化合物:雁靛黄素(Ⅰ)、高丽槐树-7-O-β-D-吡喃葡萄糖苷(Ⅱ)和芒柄花素-7-O-β-D-吡喃葡萄糖苷(Ⅲ)对人乳腺癌细胞株、宫颈癌细胞株、人肝癌细胞株生长的影响。结果化合物Ⅰ对人乳腺癌细胞株、宫颈癌细胞株的增殖均有明显的抑制作用,IC50分别为20.2、16.9μg.mL-1;化合物Ⅱ对乳腺癌细胞株、肝癌细胞株均有不同强度的抑制作用,IC50分别为17.9、18.8μg.mL-1;化合物Ⅲ对乳腺癌细胞株的增殖有抑制作用,但不呈现浓度依赖关系,对其他两个细胞株的增殖不显示抑制活性。结论化合物Ⅰ、Ⅱ对两种细胞株有不同程度的抗肿瘤活性,化合物Ⅲ不具抗肿瘤活性。  相似文献   

17.
Berberine, palmatine, and coptisine are major pharmacologically active protoberberine alkaloids in Coptidis Rhizoma, and have been used as indices for chemical evaluation of the crude drug. 1H-NMR spectroscopy was applied to determination of purities of commercial reagents of protoberberine alkaloids. The purities of the alkaloids were calculated from the ratios of the intensities of the H-13 singlet signal at about δ 8.7 ppm of target protoberberine alkaloids to integration of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability using potassium hydrogen phthalate of certified reference material (CRM) grade. The purity of the reagent estimated by the 1H-NMR was, in general, lower than that claimed by the manufacturer, leading to over-estimation of the alkaloid contents of Coptidis Rhizoma when determined by HPLC. The present quantitative 1H-NMR method was also applicable to direct determination of protoberberine alkaloid contents in Coptidis Rhizoma.  相似文献   

18.
One new aspidosperma-type alkaloid, melotenine A (1), together with five known ones (26), was isolated from the leaves of Melodinus axillaris. Their structures were elucidated by detailed spectroscopic analysis and quantum chemical ECD calculation. The isolated aspidosperma-type alkaloids were evaluated for their cytotoxicity against four human cancer cell lines and melotenine A showed significant cytotoxicity against all cells with IC50 values ranging from 0.6 to 1.5?μM.  相似文献   

19.
A chemical investigation on the 70% ethanol extract from the leaves and stems of Kopsia lancibracteolata Merr. resulted in the isolation of three new methyl chanofruticosinate type alkaloids, 12-hydroxyl prunifoline A (1), 12-hydroxyl prunifoline C (2), and N(4)-oxide prunifoline D (3). Structural elucidation of all the compounds was performed by spectral methods such as 1D- and 2D-NMR, IR, UV, and HR-ESI-MS. The isolated alkaloids were tested in vitro for cytotoxic potential against five tumor cell lines (BGC-823, HepG2, MCF-7, SGC-7901, and SK-MEL-2). As a result, alkaloid 3 exhibited significant cytotoxic activity against all tested tumor cell lines with IC50 values from 7.2 to 8.9 μM.  相似文献   

20.
Three new monoterpenoid alkaloids, melomorines A–C (13), along with melohemsine B (4), were isolated from the 70% ethanol extract of the aerial parts of Melodinus morsei. Structural elucidation of all the compounds was performed on the basis of spectral data. All the compounds were tested in vitro for cytotoxic activities. As a result, alkaloids 1, 2, and 4 exhibited cytotoxicities against all the five tested tumor cell lines with IC50 value of less than 20 μM.  相似文献   

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