首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到19条相似文献,搜索用时 187 毫秒
1.
正常人口服磷酸川芎嗪的药代动力学研究   总被引:26,自引:0,他引:26  
蔡伟  董善年  楼雅卿 《药学学报》1989,24(12):881-886
本文建立了用高效液相色谱法测定人体内川芎嗪血药浓度的方法,以C18化学键合硅胶(10μgm)为固定相,以甲醇—水(58:42)为流动相,280 nm俭测,安眠酮为内标,进行定量测定,得出俭测限为3.5 ng(S/N=4),最低检测血清浓度为17.4 ng/ml,川芎嗪血药浓度在0.029~5.82μg/ml范围内,线性关系良好,方法回收率为99.84%。方法重现性好,专一性强,内源性物质、代谢产物及同时服用的药物均不干扰。用本法测定了健康人口服川芎嗪的药代动力学参数。  相似文献   

2.
冰片和川芎嗪血药浓度的GC-MSD测定法   总被引:7,自引:0,他引:7  
建立了用气相色谱—质谱检测法同时测定血浆中冰片和川芎嗪血药浓度的方法。冰片和川芎嗪浓度在10~300ng/ml范围内线性关系良好。冰片和川芎嗪的血中回收率分别为97.32%和87.37%;最低检测浓度为2ng/ml。本方法以樟脑为内标,SIM方式检测,定量准确、干扰小,日内及日间的相对标准偏差分别小于4%和7%。并应用本法成功地进行了动物实验和健康人含服速效救心丸后冰片的药代动力学研究。  相似文献   

3.
目的建立RP-HPLC法测定兔血浆中盐酸川芎嗪和阿魏酸的方法。方法采用RP-HPLC法,使用Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相为甲醇–0.5%冰乙酸(35∶65),体积流量为1.0 mL/min,检测波长280 nm,柱温30℃,香豆素作内标,采用甲醇沉淀蛋白法处理血浆样品。结果盐酸川芎嗪线性范围为0.072~13.888μg/mL(r=0.999 8),最低检测浓度12 ng/mL,日内、日间精密度试验的RSD值均小于2%;阿魏酸线性范围为0.06~15.36μg/mL(r=0.999 9),最低检测浓度10 ng/mL,日内、日间精密度试验的RSD值均小于3%。结论该分析方法简便、灵敏,可用于同时测定兔血浆中盐酸川芎嗪和阿魏酸。  相似文献   

4.
冰片对川芎嗪血药浓度和在脑中分布的影响   总被引:14,自引:1,他引:14  
目的:探讨冰片对川芎嗪血药浓度和在脑中分布的影响。方法:经股静脉单用或复合冰片给予大鼠川芎嗪10mg/kg,采用高效液相色谱法测定不同时间的血浆和脑组织中川芎嗪药物浓度。结果:川芎嗪复合冰片后,血浆药物浓度发生明显变化,在所观察的时间里,血浆药物浓度比单用川芎嗪低;但川芎嗪复合冰片后能提高川芎嗪在脑组织中的含量。结论:冰片可促进川芎嗪在脑中的分布,川芎嗪复合冰片治疗脑血管疾病时临床显效可能更快。  相似文献   

5.
目的建立检测厄贝沙坦中潜在基因毒性杂质4’-溴甲基-2-氰基联苯的液质联用方法。方法选择三重四极杆质谱仪(电喷雾离子源ESI)进行检测,流动相为0.1%甲酸水溶液-甲醇梯度洗脱。结果该方法专属性良好;标准曲线线性范围1.25~100 ng/ml;检测限浓度为1.25 ng/ml,定量限浓度为2.5 ng/ml;精密度RSD均小于5.0%;回收率为89.2%~91.6%,准确度良好;耐用性良好。结论该方法简单灵敏准确,可以较好地满足厄贝沙坦原料生产中潜在基因毒性杂质4’-溴甲基2-氰基联苯的检测。  相似文献   

6.
目的建立高效液相色潽法测定复方川芎胶囊中川芎嗪含量的方法?椒?以十八烷基硅烷键合硅胶为填充剂,甲醇—1%醋酸钠溶液(45:55)为流动相,检测波长为302nm,流速为1.0ml/min。结果川芎嗪在5~50μg/ml浓度范围内线性关系良好,方法精密度良好,供试品溶液稳定。结论该方法能够用于复方川芎胶囊中川芎嗪的含量测定。  相似文献   

7.
反相高效液相色谱法测定人血浆中利鲁唑浓度   总被引:2,自引:0,他引:2  
温预关  莫玉泉  马崔 《中国药房》2005,16(3):203-205
目的 :建立测定利鲁唑血药浓度的方法。方法 :采用反相高效液相色谱法 ,以DiamonsilTM C18(250mm×4. 6mm ,5μm )为色谱柱 ,甲醇 -0 .03mol/L磷酸二氢铵 (80∶20 ,V/V)为流动相 ,乙醚为提取剂 ,流速为0. 8ml/min ,检测波长为265nm。结果 :利鲁唑检测浓度线性范围为5~1000ng/ml ,最低检测浓度为5ng/ml ;高、中、低3种浓度的回收率分别为99 .51 %、95. 74 %和97. 12 % ,日内RSD≤1 .17 %、日间RSD≤6. 48 % (n=5)。结论 :本方法灵敏、准确、简单、快速 ,可用于人体药动学研究。  相似文献   

8.
反相高效液相法测定盐酸曲马多血浆浓度及其药物动力学   总被引:6,自引:1,他引:5  
采用反相高效液相法(PR-HPIC)对盐酸曲马多血浆药物浓度的测定方法及药物动力学进行了研究.实验应用Waters公司HPLC系统,WatersuBONDAPAK C18柱(4.6mm×150mm);测定采用内标峰高比定量,λ为216um.本方法最佳线性关系为r=0.9991,线性范围12.5~800ng/ml,最低检测浓度为6ng/ml,最低检测量0.12ng;方法平均回收率98.08%;浓度为800.00,200.00,50.00ng/ml的日内精密度分别为7.93,7.44,13.34,日间精密度分别为0.9,10.5,13.44.药物动力学参数分别为:T_(1/2) ka=0.51±0.23h,T_(1/2)β=5.92±2.57h,Tpeak=1.67± 0.61h,C_(max)=401.74±100.05ng/ml;AUCo_(→∞)=3242.92±1618.03(ng/ml)·h.  相似文献   

9.
目的:比较化学发光法和胶体金法检测血清HBsAg结果及发光法检测浓度和胶体金法检测血清HBsAg时间关系。方法:化学发光法是使用双抗夹心法定量检测人血清或血浆中的HBsAg含量;胶体金法是采用抗原抗体反应原理及胶体金标记免疫层析分析技术。用胶体金法检测经化学发光法从4323人中筛选的342例临床标本模式如下:①HBsAg浓度大于21ng/ml的261例;②6.4~21ng/ml的27例;③4.8~6.4ng/ml的22例;④0.51~4.8ng/ml19例;⑤0.16~0.5ng/ml9例;⑥小于0.15ng/ml但HBeAg和HBcAb阳性的4例。结果:化学发光法灵敏度0.05,阳性检出率98.4%;胶体金法10分钟内的灵敏度21.00阳性检出率76.3%;15分钟内的灵敏度6.4阳性检出率84.2%;20分钟的灵敏度为4.8阳性检出率90.6%;30分钟的灵敏度为0.50,阳性检出率96.2%.结论:胶体金法灵敏度不如化学发光法,受判读时间和人观察因素影响大,易漏检;而化学发光法灵敏度高,漏检率低。  相似文献   

10.
目的:探讨血清降钙素原(PCT)检测对感染性疾病的诊断及治疗的应用价值,为血清降钙素原在感染性疾病诊治中的临床应用提供科学依据。方法:本次研究对2013年1月至2014年12月来我院就诊的90例感染性疾病患者,采用上转发光法检测血清降钙素原PCT水平,统计分析该90例感染性疾病患者血清降钙素原PCT水平。检测结果分4个等级:分别是<0.5ng/ml;0.5ng/ml-2ng/ml;2ng/ml-10ng/ml;>10ng/ml。结果:以血清降钙素原PCT水平≥0.5ng/ml为阳性阈值,我们发现:细菌感染患者血清降钙素原PCT阳性率为86.96%,且浓度高,多超过2.0ng/ml。而病毒感染组血清降钙素原PCT阳性率为11.36%,且浓度相对较低,以0.5ng/ml-2ng/ml为主。两组之间的差异有统计学意义(P<0.05)。结论:血清降钙素原(PCT)是感染性疾病诊断和治疗中一项重要的指标,其值高低可以作为是否使用抗菌药物的依据。  相似文献   

11.
Objectives To investigate the effect of the Xiongbing compound (XBC) on the pharmacokinetics and brain targeting of tetramethylpyrazine (TMP). Methods Three microemulsions containing the same TMP concentration were prepared. XBC microemulsions were made from Rhizoma ligustric Chuanxiong extracts, borneol and TMP. TMP microemulsions were made with TMP only. Borneol microemulsions contained borneol and TMP. Microdialysis with high performance liquid chromatography (HPLC) was used to measure the concentration of TMP in the blood and striatum after intravenous (i.v.) or intragastric (i.g.) administration of the three different microemulsions. Key findings The pharmacokinetics of free TMP concentration in the blood and the striatum fit a first‐order rate, open two‐compartment model after intravenous and intragastric microemulsion administration. The maximal concentration (Cmax) and area under curve (AUC) values in the XBC microemulsion i.v. group were significantly higher than that in the TMP microemulsion and borneol microemulsion i.v. groups. After XBC microemulsion i.g. administration, the t1/2, mean residence time (MRT) and AUC of TMP in both plasma and brain tissues were greater than those with TMP microemulsion and borneol microemulsion administration. The relative brain targeting efficiency of TMP for the XBC microemulsion i.v and i.g. groups relative to the TMP microemulsion and borneol microemulsion groups were greater than 1. Conclusion XBC microemulsion can enhance TMP oral bioavailability, brain targeting and tissue distribution, mainly through a synergistic action of Rhizoma ligustric Chuanxiong extracts and borneol.  相似文献   

12.
王晖  赵继会  苏晓霞  冯年平 《中国药房》2012,(19):1768-1770
目的:建立同时测定复方微乳中川芎嗪和冰片含量的方法。方法:采用气相色谱法。色谱柱为HP-INNOWAX 19091N-113(30m×0.32mm×0.25μm),内标为水杨酸甲酯,检测器为火焰离子化检测器(FID),柱温采用程序升温,进样口和检测器温度分别为230℃和250℃。结果:川芎嗪和冰片检测浓度的线性范围分别为4.9~156.8、5.3~169.6μg·mL-1(r均为0.9996);平均加样回收率分别为98.09%(RSD=1.75%,n=9)和98.39%(RSD=1.17%,n=9)。结论:本方法简便、快速,灵敏、专属性好,可用于复方微乳中川芎嗪和冰片的含量测定。  相似文献   

13.
Xu X  Li Y  Hou J  Zhang S  Xu Y  Wang Y  Zhang Y  Liu C  He X 《Planta medica》2011,77(14):1600-1604
Both borneol and menthol are bioactive substances derived from Chinese herbal medicines. In order to understand the pharmacokinetics of borneol and menthol in Qingyan drop pills, a rapid, sensitive, and simple gas chromatographic (GC) method with flame ionization detection (FID) was developed for the simultaneous determination of borneol and menthol in rat plasma. Sample preparations were carried out by liquid-liquid extraction (LLE) with an internal standard solution of naphthalene. The analytes and internal standard (IS, naphthalene) were separated well on an HP-1 capillary column. The pharmacokinetic parameters were estimated by a compartmental method using the Phoenix WinNonlin software program (Version 6.0). The standard curves were linear over a wide concentration range of 2.5-50.0?ng/μL ( R?=?0.9963), 8.7-62.2?ng/μL ( R?=?0.9994) for both borneol and menthol in plasma, respectively. The limits of quantification (LOQ) of borneol and menthol in plasma were 2.4?ng/μL and 5.0?ng/μL, respectively. The intra-day precisions for borneol and menthol were?相似文献   

14.
A simple and reproducible high-performance liquid chromatographic method was developed for simultaneous determination of sulfamethoxazole (SMX) and trimethoprim (TMP) in human plasma. The method entailed injection of the samples after deproteination with perchloric acid and subsequent neutralizing. Primidone was used as internal standard. Chromatography was performed on a C(18) column (250 mm x 4.6 mm, 5 microm) under isocratic elution with 50 mM aqueous sodium dihydrogen phosphate-acetonitrile-triethylamine (100:25:0.5, v/v), pH 5.9. Detection was made at 240 nm and analyses were run at a flow-rate of 1.5 ml/min at a temperature of 35 degrees C. The recovery was 83.4, 88.5 and 98.2% for TMP, SMX and internal standard, respectively. The precision of the method was 2.6-9.8% over the concentration range of 0.125-2 microg/ml for TMP and 0.39-50 microg/ml for SMX. The limit of quantification (LOQ) in plasma was 0.125 and 0.39 microg/ml for TMP and SMX, respectively. The method was used for a bioequivalence study.  相似文献   

15.
汪乃兴  谢振贲 《药学学报》1987,22(11):848-853
本文用微分脉冲极谱法和循环伏安法研究了甲氧苄氨嘧啶(TMP)的电化学行为,选择了最佳的测定条件,TMP的检出限可达2.0×10-7mol/L,方法灵敏度较高,并研制了TMP-PVC膜离子选择性电极,试验了该电极的各种特性及Nernst响应范围。本法应用于制剂中TMP的测定,选择性好,干扰少,毋须分离,方法简便快速。选择电极在人尿和血清的介质中测定,也能获得良好的结果。  相似文献   

16.
The combination of sulfadoxine (SDO) with trimethoprim (TMP) is widely used in veterinarian medicine. The aim of the present study was to compare excretion profiles and detection time windows of SDO and TMP in plasma and urine by means of a validated quantitative method. Eight horses received a single intravenous (i.v.) dose of 2.7 mg TMP and 13.4 mg SDO per kg bodyweight. Plasma and urine samples were collected up to 15 and 70 days post-administration, respectively. While urine samples underwent an enzymatic hydrolysis, plasma samples were proteolysed before further analysis. After solid-phase extraction, samples were analysed by liquid chromatography/electrospray ionisation tandem mass spectrometry in positive ionisation mode. The applied multiple reaction monitoring (MRM) method allowed the detection of SDO and TMP with a lower limit of detection of 0.03 ng/mL in plasma and 0.2 (SDO) and 0.4 ng/mL (TMP) in urine, respectively. In the present study, detection times for SDO were 15 days in plasma and 49 days in urine, respectively. TMP was detected for up to 7 days in plasma and up to 50 days in urine, respectively. The detection via the TMP metabolite 3-desmethyl-trimethoprim was possible for 70 days in urine. Detection times of the other confirmed metabolites N4-acetylated sulfadoxine, hydroxytrimethoprim, trimethoprim-1-oxide and trimethoprim-3-oxide were significantly lower. In order to postulate reasonable screening limits (SLs) to control specific withdrawal times, a Monte Carlo simulation was performed for SDO. The proposed SL of 10 ng/mL SDO in blood and 300 ng/mL urine corresponds to a detection time of 4 days.  相似文献   

17.
毛细管柱气相色谱法测定冠心苏合胶囊中的冰片含量   总被引:1,自引:0,他引:1  
目的建立冠心苏合胶囊中冰片的含量的测定方法。方法以水杨酸甲酯为内标物,采用内标法测定;选择FID检测器、SE-54毛细管柱气相色谱法测定冠心苏合胶囊中冰片的含量。结果在该色谱条件下,样品中冰片与内标物能得到很好的分离,冰片在0.2~2mg/ml范围内线性关系良好(r=0.9997,n=6),加样回收率为96.93%(RSD=1.1%,n=5)。结论本方法灵敏、准确、重现性好,可用于该制剂中的冰片含量测定。  相似文献   

18.
The potential interaction between the nucleoside analog emtricitabine (FTC) and trimethoprim (TMP) was assessed in the isolated perfused rat kidney (IPK) model and in vivo in rats. IPK experiments were performed with FTC alone (2 microg/mL) and in the presence of increasing concentrations of TMP (1-10 microg/mL). TMP inhibited FTC excretion in a concentration dependent manner. The IC(50) (TMP concentration associated with a 50% reduction in FTC excretion) was 1.86 +/- 0.37 microg/mL. The results were compared to whole animal studies in rats. Animals received an IV dose of FTC (1 mg/kg) with or without pretreatment with TMP (25 mg/kg). TMP coadministration significantly decreased FTC clearance (7.4 +/- 1.2 mL/min/kg to 2.7 +/- 0.53 mL/min/kg), and elimination half-life was significantly increased (58 +/- 12 min to 215 +/- 44 min). A good correlation was obtained between IPK findings and in vivo data, as FTC renal clearance was reduced approximately 60% in the presence of TMP in both studies. Based on this investigation, TMP would be expected to inhibit the renal excretion of FTC when the two compounds are coadministered, resulting in increased plasma exposure of FTC. However, the clinical significance of this finding remains to be elucidated.  相似文献   

19.
目的用气相色谱法测定双柏膏中的冰片含量。方法以水杨酸甲酯为内标物,采用内标物预先加入法,弹性石英毛细管柱Supelcowax-10(30m×0.32mm,0.25μm),FID检测器,氮气为载气,柱温为110℃。结果在该色谱条件下,冰片中异龙脑和龙脑及内标物水杨酸甲酯均得到良好的分离,冰片的回收率为103.4%(RSD=0.4%)。结论该法灵敏、准确,重复性好,可用于控制该制剂的质量。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号