轮环藤中生物碱成分的研究

目的 研究轮环藤根中的生物碱成分.方法 采用多种色谱手段分离纯化,并通过波谱技术及理化性质鉴定化合物结构.结果 从轮环藤根中分离得到8个生物碱成分,分别鉴定为异粒枝碱(Ⅰ)、轮环藤碱(Ⅱ)、海岛轮环藤碱(Ⅲ)、海岛轮环藤酚碱(Ⅳ)、racemosinine B(Ⅴ)、木兰碱(Ⅵ)、α-cyclanoline(Ⅶ)和steponine(Ⅷ).结论 化合物Ⅳ～Ⅷ均为首次从轮环藤中分离得到.     

毛苞瓜叶乌头生物碱成分的研究

目的 研究毛苞瓜叶乌头根中的生物碱化学成分.方法 酸提碱沉提取总碱,硅胶层析法分离,波谱法鉴定结构.结果 分离到6个已知的去用二萜生物碱,分别为滇乌碱(yunaconitine)、塔拉萨敏(talasamine)、瓜乌定(hemsleyadine)、拳乌宁乙(circinanine B)、黄草乌碱丁(schaconitine)和senbusine A.结论 应用光谱法和与已知物TLC对照的方法鉴定了所有化合物的结构.     

钩藤叶化学成分的研究

目的 研究茜草科植物钩藤Uncaria rhynchophylla(Miq.)Jacks.叶的化学成分.方法 用多种色谱方法和重结晶法进行分离和纯化,经理化、光谱和色谱分析等方法鉴定结构.结果从钩藤叶中分离得到5个化合物,鉴定为喜果苷(vincoside lactam,Ⅰ)、异去氢钩藤碱(isocorynoxeine,Ⅱ)、异钩藤碱(isorhynchophylline,Ⅲ)、去氢钩藤碱(corynoxeine,Ⅵ)、钩藤碱(rhynchophylline,Ⅴ).结论化合物Ⅲ为首次从钩藤叶中分离得到,通过2D-NMR技术完善了化合物Ⅲ的核磁数据.     

锥花黄堇中的异喹啉生物碱

锥花黄堇(Corydalis thyrsiflora Prain)系罂粟科(Papaveraceae)紫堇属(Corydalis)植物,在西藏地区以其全草入药,藏药名“抓桑”,用于镇痛。其化学成分未见文献报道。我们用离子交换树脂法从该植物全草中提出总生物碱,通过柱层析及制备薄层层析分离,得到12个化合物并鉴定了结构,均为异喹啉生物碱(见图1),分别为原阿片碱     

粉防己生物碱化学成分的分离与鉴定

目的对防己科千金藤属植物粉防己(Stephania tetrandraS.Moore)的生物碱化学成分进行研究。方法采用反复硅胶柱色谱法、Sephadex LH-20柱色谱法等进行分离纯化,并通过光谱分析鉴定其化学结构。结果分离得到7个生物碱类化合物,分别鉴定为粉防己碱(tetrandrine,1)、防己诺林碱(fangchinoline,2)、2′-N-chloromethyltetrandrine(3)、氧化防己碱(oxofangchirine,4)、粉防己碱D盐酸盐(fenfangjine D hydrochloride,5)、荷苞牡丹碱((+)-dicentrine,6)、tazopsine(7)。结论化合物7为首次从该属植物中分离得到,化合物6为首次从该植物中分离得到。     

三小叶翠雀花中生物碱成分的研究

目的　研究三小叶翠雀花(ＤｅｌｐｈｉｎｉｕｍｔｒｉｆｏｌｉｏｌａｔｕｍＦｉｎｅｔｅｔＧａｇｎｅｐ)全草中的生物碱化学成分。方法　离子交换树脂法提取总碱,硅胶层析法分离,波谱法鉴定结构。结果　从中分得10个已知Ｃ19-二萜生物碱。分别为氨茴酰牛扁碱(ａｎｔｈｒａｎｏｙｌｌｙ ｃｏｃｔｏｎｉｎｅ)1、牛扁碱(ｌｙｃｏｃｔｏｎｉｎｅ)2、ｄｅｌｓｅｍｉｎｅＡ(3)、ｄｅｌｓｅｍｉｎｅＢ(4)、ｇｉｒａｌｄｉｎｅＧ(5)、ｊｉｕｆｅｎｇｄｉｎｅ(6)、ｂｒｏｗｎｉｉｎｅ(7)、14-ｄｅｈｙｄｒｏ ｂｒｏｗｎｉｉｎｅ(8)、ｄｅｌｓｏｌｉｎｅ(9)和ｄｅｌｐｈａｔｉｎｅ(10)。结论　应用光谱法和与已知物ＴＬＣ对照的方法鉴定了所有化合物的结构。     

百蕊草生物碱成分的分离与鉴定

目的 研究百蕊草中的生物碱成分.方法 应用多种色谱手段进行分离纯化,通过IR、MS、HR-MS、NMR、2D-NMR技术鉴定化合物结构.结果 从百蕊草总生物碱部位分离得到3个化合物,分别鉴定为N-甲基金雀花碱(1)、白金雀儿碱(2)和槐果碱(3).结论 化合物N-甲基金雀花碱、白金雀儿碱、槐果碱均为首次从该植物中分离得到.     

宣威乌头中生物碱成分的研究

目的 研究宣威乌头Aconitum nagarumvar lasiandrum中的生物碱成分.方法 采用酸提碱沉法提取总碱、硅胶层析法分离,用波谱法鉴定结构.结果和结论 从宣威乌头中分得10个单体化合物,应用光谱法鉴定了其结构为:光翠雀碱(denudatine)、宋果灵(songorine)、delnuttaline等3个C20-二萜生物碱和尼奥灵(neoline)、3-去氧乌头碱(3-deoxyaconitine)、8-methoxy-14-benzoylaconine、伏乌碱(flavaconitine)、异塔拉萨敏(isotalatizidine)、乌头碱(aconitine)和nevadensine等7个C19-二萜生物碱.     

峨眉唐松草化学成分的研究

从四川产峨眉唐松草(Thalictrum omeiense W.T.Wang)根茎中分离得到八种生物碱(Ⅰ～Ⅷ),碱Ⅰ,Ⅱ,Ⅴ,Ⅶ和Ⅷ分别鉴定为oxyberberine(Ⅰ),thaliadine(Ⅱ),thalmelatidine(Ⅴ),adiantifoline(Ⅶ)和thalmineline(Ⅷ)。碱Ⅵ为新的二聚阿朴芬—苄基异喹啉类生物碱,根据理化性质和光谱数据所推定的结构如Ⅵ所示。命名为峨眉唐松草碱(methoxyadiantifoline)。碱Ⅲ和碱Ⅳ的结构尚在研究中。药理实验结果表明峨眉唐松草碱具有较明显的扩张冠状动脉作用。     

黑水翠雀中生物碱成份的研究

目的：研究黑水翠雀（Delphinium potaninii)根中生物碱成分,方法：采用PH梯度洗脱及硅胶柱层析。结果：从根中分得10个已知二萜生物碱,由光谱法分别鉴定为牛扁碱（lycoctonine)1、氨茴牛扁碱（anthranoylycoctonine)2、methyllycaconitine3、德尔塔生（deltatsine)4、delectine5、delsoline6、德尔色明甲(delsemineA)7、德尔色明乙（delsemineB)8、德拉瓦印甲和乙（delavaineA and B)9 10。结论这些化合物是首次从该植物中分离得到。     

巴豆属植物Croton hemiargyerius var.gymnodiscus中生物碱的研究

目的 从巴西巴豆属药用植物的一新变种复制Muel的叶中分离生物碱成分。方法 应用硅胶柱层析法分离和纯化，波谱分析与已知化合物的波谱和理化性质比较得以确定化合物结构。结果 分离得到12个生物碱，其中4个为新生物碱，命名为hemiargineA(1)，B(5)，C(6)和D(7)；8个已知生物碱为isocorydine(2)，corydine(3)，norcorydine(4)，salutaridine(8)，glaucine(9)，tetrahydropalmatrubine(10)，xylopinoine(11)和norlaudanosine(12)。结论 获得4种新化合物，除生物碱(8)，其余生物碱均为首次从该属植物中获得。     

Chemical Study of Alkaloids from Corydalis bungeana

From the herbs of CORYDALIS BUNGEANA, 16 alkaloids were isolated; 3 of them were identical with acetylcorynoline ( 2), corynoline ( 5), protopine ( 9), which had been obtained before from this plant. A further 11 known alkaloids: dihydrosanguinarine ( 1), acetylisocorynoline ( 3), 11-epicorynoline ( 4), corycavine ( 6), bicuculine ( 7), 12-hydroxycorynoline ( 8), scoulerine ( 12), cheilanthifoline ( 13), yuziphine ( 14), isoboldine ( 15), noryuziphine ( 16) were isolated for the first time from this plant. The tenth, named (+)-bungeanine, is a new alkaloid. Its structure has been established to be N-nor-5,14-dehydroacetylcorynoline. The conformation of (+)-bungeanine in solution is also discussed.     

Cytotoxicity and DNA topoisomerase inhibitory activity of constituents isolated from the fruits ofEvodia officinalis

Four alkaloids (1-4), three quinolone alkaloids (5-7), and three flavanoid glucosides (8-10) were isolated from the fruits of Evodia officinalis Dode, and their structures were determined from chemical and spectral data. Compounds, 3, 8, 9 and 10 were isolated from this plant for the first time. Of these compounds, 1-3 and 5-7 exhibited moderate cytotoxicities against cultured human colon carcinoma (HT-29), human breast carcinoma (MCF-7), and human hepatoblastoma (HepG-2). Compound 8 showed strong inhibitory effects on DNA topoisomerases I and II (70 and 96% inhibition at a concentration of 20 microM, respectively).     

Chemical Study on Alkaloids from Corydalis bulleyana.

Twenty three isoquinoline-type alkaloids were isolated from whole plant of CORYDALIS BULLEYANA Diels. Sixteen of them were identified as known alkaloids, namely, protopine ( 1), (+)consperine ( 2), (+)acetylcorynoline ( 3), dihydrosangunarine ( 4), (+)acetylisocorynoline ( 6), (+/-)stylopine ( 7), (+)corynoline ( 8), (+)corynoloxine ( 9, (+)isocorynoline ( 10), (-)chelanthifoline ( 13), corycavamine ( 14), (+)scoulerine ( 15), (+)isoboldine ( 16), acetylcorydamine ( 18), allocryptopine ( 21) and corydamine ( 23). The remainder were new alkaloids and were referred to as bulleyanine [(6-acetonylacetylisocorynoline)] ( 5), (+)6-acetonylcorynoline ( 11), (+)12-formyloxycorynoline ( 12), (+)6-oxoacetylcorynoline ( 17), (+)12-hydroxycorynoline ( 19), (+)bulleyanaline [(+)6-acetonyl-12-hydroxycorynoline] ( 20) and (+)norjuziphine ( 22), respectively. Their structures have been elucidated by means of spectral and chemical methods.     

聚伞翠雀生物碱成分的研究

目的研究聚伞翠雀根中生物碱成分。方法采用pH梯度洗脱及硅胶柱层析分离,应用光谱方法鉴定结构。结果从根中分得5个已知二萜生物碱:氨茴酰牛扁碱、牛扁碱、德尔塔生、DelsemineA和DelsemineB。结论5个化合物是首次从该植物中分离得到。     

松潘乌头二萜生物碱的研究

甘肃省文县产的松潘乌头(Aconitum sungpanense Hand-Mazz),俗称缠绕乌头,民间用于治疗跌打损伤,风湿性关节痛等症。其化学成分尚未见报道。松潘乌头粗粉用0.5%的盐酸渗漉,通过阳离子树脂交换,经乙醚及二氯甲烷先后洗脱,乙醚部分用硅胶柱层析等     

Chemical constituents from the<Emphasis Type="Italic">Hydrangea chinensis</Emphasis>

Two quinazolone alkaloids, (+)-febrifugine (1) and isofebrifugine (2), along with three coumarin derivatives, 6-hydroxy coumarin (3), skimmin (5), and umbelliferone-7-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranoside (6), were isolated from the roots of Hydrangea chinensis. Compound 6 is a new compound. In addition, umbelliferone (4), linoleic acid (7), two steroidal glycosides (8, 9), three furfural derivatives (10-12), and butyl-beta-D-fructofuranoside (13) were isolated from the leaves of the same plant. The structures of all isolates were elucidated by spectral methods.     

Phytochemical investigation and in vitro cytotoxic evaluation of alkaloids from Abuta rufescens

A phytochemical investigation of Abuta rufescens was performed to authenticate plant material reported previously and to assess the cytotoxicity of the alkaloids obtained from the plant. Three alkaloids which have not previously been reported from this species, two phenolic (subsessiline, an oxoaporphine, and telitoxine, an azafluoranthene) and one non-phenolic (isoimerubrine, a tropoloisoquinoline), were isolated and identified. These alkaloids, along with others previously isolated from this and another Abuta species (grandirubrine, a tropoloisoquinoline), were evaluated for cytotoxic activity against several human cancer cell lines (HCT-116 colon adenocarcinoma, ACHN renal carcinoma, and A549 lung carcinoma). The tropoloisoquinoline alkaloids (grandirubrine, imerubrine, and isoimerubrine) exhibited the greatest cytotoxicity against the cell lines, especially ACHN and HCT-116 cells. The azafluoranthene alkaloid imeluteine exhibited lesser cytotoxicity, as did one of the oxoaporphine alkaloids.