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1.
目的探讨乌贼墨对小鼠造血干细胞、粒-单系祖细胞及外周血WBC的影响。方法用不同剂量的乌贼墨灌胃正常小鼠、环磷酰胺(Cy)和辐射骨髓损伤模型小鼠,采用造血祖细胞体外培养方法和实验血液学技术,检测小鼠骨髓造血干细胞生成数(CFU-S)、粒-单系祖细胞集落生成数(CFU-GM)和外周血WBC数量。结果乌贼墨能够显著提高正常小鼠CFU-S,CFU-GM和外周血WBC数量,有效拮抗模型小鼠体内CFU-S,CFU-GM及外周WBC的降低,并显著促进模型小鼠上述各指标的恢复。结论乌贼墨具有显著的促进小鼠骨髓粒系造血作用。其作用机制可能通过调节机体的免疫机能,诱导机体产生粒/单核细胞集落刺激因子和多种细胞因子,促进造血细胞的增殖,并诱导造血细胞向粒单系细胞分化。  相似文献   

2.
乌贼墨对小鼠NK细胞杀伤活性的影响和抑瘤作用的研究   总被引:8,自引:0,他引:8  
研究乌贼墨对小鼠脾细胞NK细胞杀伤活性的诱生作用及NK细胞杀伤性的持续时间,并探讨乌贼墨对荷瘤鼠脾细胞NK细胞杀伤活性的影响及抑制肿瘤生长的作用。用5%的乌贼墨给小鼠灌胃,连续5d后取不同时间的小鼠脾细胞,应用乳酸脱氢酶释放法检测NK细胞杀伤活性,结果表明,口服乌贼墨后可使小鼠NK细胞杀伤活性增强,实验组与对照组比较有显著性差异(P<0.01),诱生后24h杀伤活性明显增强,2周左右恢复正常水平,乌贼墨可使荷瘤鼠NK细胞杀伤活性增强,瘤体明显小于阴性对照组,病理学分析证明小鼠瘤组织出血坏死及炎细胞浸润程度明显高于对照组,阳性对照组小鼠免疫功能损伤严重。  相似文献   

3.
乌贼墨对小鼠肿瘤坏死因子的诱生作用   总被引:12,自引:5,他引:7  
用乌贼墨喂养小鼠后采集血清,经体外检测对L929细胞的细胞毒作用表明:乌贼墨在动物体内具有明显的肿瘤坏死因子诱生作用。  相似文献   

4.
乌贼墨诱导小鼠巨噬细胞产生白细胞介素-1的研究   总被引:6,自引:1,他引:6  
目的:应用乌贼墨,研究其诱导BALB/C鼠巨噬细胞产生白细胞介素-1(interleukin-1)。方法:给小鼠ig灌入乌贼墨,抽取腹腔液,应用成纤维细胞增殖实验检测诱导巨噬细胞产生IL-1的生物学活性,体内持续时闰争不同稀释浓度促细胞增殖的结果。结果:乌贼墨能诱导BALB/C鼠巨噬细胞产生IL-1,且分泌活性较强,与对照组比较有显著性差异。体内跟踪检测可见停止诱生后第2天达高峰,第5天分泌活性仍明显,第9天接近正常。lL-1待检液稀释8倍时活性无明显变化,32倍时近正常水平。结论:乌贼墨能诱导小鼠巨噬细胞产生IL-1,48h分泌活性最高。  相似文献   

5.
目的探讨乌贼墨促进免疫低功小鼠免疫功能的作用。方法按0.1,0.25和0.5mL剂量给C57BL/6小鼠灌胃5%的新鲜乌贼墨,每日1次,连续7d,并在给药的第1日和第2日同时腹腔注射环磷酰胺造成免疫功能低下模型,第9日检测小鼠免疫功能的变化。结果乌贼墨可促进免疫低功小鼠的脾淋巴细胞增殖;增强NK细胞杀伤活性;提高免疫效应分子TNF-α,IFN-γ及一氧化氮(NO)的产生水平。结论乌贼墨可增强固有免疫细胞和特异性免疫细胞的活性,调节细胞因子和效应分子的分泌,刺激免疫低功小鼠的免疫功能恢复。  相似文献   

6.
目的:考察复方乌贼墨胶囊对延缓衰老的影响。方法:果蝇生存试验;小鼠血和组织中丙二醛、SOD和谷胱甘肽过氧化物酶测定。结果:复方乌贼墨胶囊对果蝇的平均寿命天数有明显延长作用;对消除小鼠体内脂质过氧化降解产物效果显著并能增强SOD活性。结论:复方乌贼墨胶囊具有延缓衰老作用。  相似文献   

7.
《中国海洋药物》2009,28(1):36-40
目的探讨乌贼墨提取物对环磷酰胺所致小鼠肺纤维化的改善或治疗作用及在不同乌贼墨提取物浓度条件下的保护效果。方法腹腔注射环磷酰胺以制作肺纤维化模型,试验组灌服不同剂量的乌贼墨提取物,观察小鼠肺重系数,血液与肺组织中MDA、SOD、HYP、CAT及GSH-Px含量。结果与对照组相比,环磷酰胺能显著地改变小鼠肺重系数(P<0.01),也能不同程度地改变小鼠血液及肺组织中MDA、SOD、HYP、CAT及GSH-Px的含量,从而促进肺的纤维化过程。适当剂量的乌贼墨提取物能显著改善由环磷酰胺所致肺纤维化小鼠各项指标的变化(P<0.05或P<0.01),但在提取物浓度太高的情况下,对环磷酰胺所致肺纤维化小鼠各项指标变化的影响较小。结论乌贼墨提取物对环磷酰胺引起的小鼠肺纤维化具有一定的保护作用。  相似文献   

8.
乌贼墨诱生小鼠IFN-γ及调节LAK细胞活性的研究   总被引:9,自引:0,他引:9  
为进一步研究乌贼墨对免疫功能的刺激作用 ,本文采用 ELISA技术和生物活性测定技术分别检测了经乌贼墨灌胃后小鼠血清中的 IFN- γ的水平和 L AK细胞活性的变化。结果显示 ,实验组小鼠 IFN- γ的水平于乌贼墨灌胃后 2 4 h开始升高 ,4 8h达高峰 ,72 h降至正常水平。L AK细胞杀伤活性也有明显增强 ,与对照组小鼠相比 ,差异显著 (P<0 .0 1)。结论进一步证实了乌贼墨可诱导机体产生多种细胞因子和促进免疫活性细胞作用的发挥  相似文献   

9.
目的:探讨乌贼墨对脾细胞和巨噬细胞NO生成及IFN-γ分泌的影响。方法:用Griess法和ELISA法分别检测了经乌贼墨灌胃处理后小鼠脾细胞和腹腔巨噬细胞培养上清中NO和IFN-γ的水平的变化。结果:乌贼墨灌胃处理小鼠后的第4、6天脾细胞及腹腔巨噬细胞(Mφ)可产生较高水平的NO;脾细胞可产生较高水平的IFN-γ,并且小鼠脾细胞NO的产生水平与IFN-γ产生水平呈正相关(r=0.98);用LPS和L-NMMA(NO抑制剂)分别同乌贼墨灌胃第6d小鼠的脾细胞一起培养,结果显示LPS可协同IFN-γ促NO水平升高。L-NMMA可抑制小鼠脾细胞的NO生成。结论:乌贼墨可促进巨噬细胞NO生成;脾细胞NO的产生与IFN-γ分泌水平呈正相关。  相似文献   

10.
乌贼墨抗肿瘤机制研究进展   总被引:1,自引:0,他引:1  
李孝东  袁建华 《中国药师》2003,6(10):652-654
乌贼属于软体动物门 ,头足纲的一种海洋动物 ,其医药应用历史悠久 ,乌贼骨 (药名海螵蛸 )可调节胃酸分泌、止血等 ;乌贼肉具有活血化瘀的功效 ;乌贼墨在《本草拾遗》中有“腹中墨 ,主治血刺心痛”的记载 ,对功能性子宫出血、肺结核咯血等出血性疾病有止血作用 ;2 0世纪 90年代日本学者发现乌贼墨中含有一种肽聚糖对Meth A肿瘤的抑制率达6 4 % [1] 。国内吕昌龙等[2 ] 用乌贼墨对小鼠连续灌胃 5d ,发现乌贼墨可明显抑制Meth A、S180两种肉瘤的生长 ,抑制率分别为 95 .4 8%和 73.91% ,并一定程度上抑制移植瘤的形成。关于乌贼墨的抗肿瘤机制…  相似文献   

11.
目的 通过观察sOGP在体内的促造血活性和体外促进造血祖细胞增殖的作用 ,初步探索sOGP促造血活性的作用环节及机制。方法 正常小鼠连续 14d皮下注射sOGP ,观察外周血象、骨髓有核细胞数及骨髓有核细胞分类计数的变化 ;又应用离体骨髓细胞培养方法观察sOGP对正常小鼠和人骨髓粒系祖细胞集落形成的影响。结果 sOGP能升高正常小鼠骨髓有核细胞数和外周血白细胞数分别为2 0 %和 40 % ,红细胞和血小板也有增加趋势 ,骨髓细胞增生较空白对照组活跃 ,粒系增生较明显。体外CFU G集落培养实验进一步证实了sOGP具有直接刺激小鼠和人骨髓粒系祖细胞增殖的作用 ,1× 10 -14 mol·L-1浓度时已有明显的效果 ,sOGP和阳性对照药rhG CSF在相近浓度时效果相接近。结论 sOGP可能具有促进小鼠骨髓全系细胞增生的作用 ,以促进粒系造血为主 ,其机制可能是直接作用于造血干 /祖细胞或改善造血微环境促进造血 ,提示sOGP在促进放 /化疗患者造血功能恢复等方面潜在的应用价值  相似文献   

12.
地黄低聚糖对快速老化模型小鼠造血功能的影响   总被引:14,自引:1,他引:14  
目的:研究地黄低聚糖(RGOS)对快速老化模型小鼠(SAMP8)造血功能的影响。方法:采用造血祖细胞体外克隆培养等实验血液学方法。结果:RGOS10,20mg·kg-1可使SAMP8小鼠的CFU-S、CFU-GM、CFU-E和BFU-E明显增多,并可使外周血中降低的WBC数明显增加。提示RGOS可刺激SAMP8小鼠造血干细胞、祖细胞的增殖分化。集落刺激活性实验显示RGOS可使小鼠体内的集落刺激因子产生明显增多。小鼠骨髓组织学研究结果也进一步证实了RGOS增强SAMP8小鼠造血功能的作用。结论:RGOS可以增强SAMP8小鼠的造血功能。  相似文献   

13.
巴西蘑菇多糖对造血干、祖细胞生成的影响   总被引:4,自引:0,他引:4  
目的:探讨国产巴西蘑菇多糖对造血干、祖细胞生成的影响及其作用机制。方法:应用造血细胞培养技术、流式细胞仪(FACS)、逆转录多聚酶链反应(RT-PCR)观察药物对小鼠骨髓细胞及人脐血单个核细胞的影响。结果:经过巴西蘑菇多糖处理的动物,造血干细胞(CFU-S)、粒-单祖细胞(CFU-GM)、红系祖细胞(CFU-E)和纤维母细胞(CFU-F)各组数值明显上升,与对照组比较,P均<0.01;FACS检测显示,实验组CD33+细胞明显上升,在诱导的d 7达高峰;经RT-PCR检测结果表明,巴西蘑菇多糖诱导人脐血单个核细胞48 h后,粒单细胞克隆刺激因子(GM-CSF)mRNA的表达量是对照组的2.72倍。结论:巴西蘑菇多糖可促进造血干、祖细胞生成并可促进人脐血单个核细胞GM-CSF基因表达。  相似文献   

14.
硫芥对小鼠造血干细胞自我更新和分化功能的影响   总被引:1,自引:0,他引:1  
用2次移植法测定不同剂量硫芥sc小鼠骨髓CFU_0的自我更新和分化功能。硫芥小剂量(15mg/kg)杀伤下,约半数剩余的CFUs的更新潜能仍保持正常。加大硫芥剂量(25mg/kg)使CFUs严重耗竭,残存的CFUs仅为正常值的8%,但其自我更新能力却高达正常的2.5倍,并历时30d而不减退。在上述剂量作用下,不论近期或远期测定,残存的CFUs主要表现为向粒系分化的提高和向巨核系分化的减少。硫芥作用下残存的CFUs具有良好的自我更新功能,是其染毒后造血功能得以迅速恢复的根本原因。然而,造血干细胞向巨核系分化的相对减少,可能影响血小板的生成量是应该引起注意的问题。  相似文献   

15.
Pharmacokinetic studies of five biological response modifiers (BRMs) show diverse regulator functions on effector cell responses and a capacity to cause the secretion of specific soluble factors. Of the five BRMs tested, MVE-2, Poly ICLC, OK-432, and alpha beta IFN were capable of stimulating both natural killer (NK) cell and macrophage (M phi) tumoricidal activity, whereas BM 41-332 augmented only NK cells. Following one treatment with the aforementioned BRMs, M phi activity remained elevated for a longer period (10-14 days) than did NK cell activity (6-9 days). Of particular interest, multiple treatment with BRMs led to a downregulation of NK cell activity (hyporesponsiveness). Three soluble secretory products were induced by these BRMs (colony stimulating factor, CSF; prostaglandin E, PGE; and interferon, IFN). Treatment with MVE-2 and Poly ICLC led to a significant increase in bone marrow cellularity and GM-CFV-C. Results of studies with the cyclo-oxygense-inhibited indomethacin indicate that CSF and PGE are produced and/or secreted by different cellular mechanisms. The depression of effector cells (NK, bone marrow, and GM-CFU-C), as the result of cyto-reductive treatment with cyclophosphamide, could be reversed by treatment with MVE-2. A significantly earlier time to recovery of these effector cells was achieved following MVE-treatment. When MVE-2 was used as an adjuvant to initial tumor cyto-reductive chemotherapy, more successful antitumor response was achieved, indicating that MVE-2 functioned to elevate a substantial effector cell response as well as reconstituting bone marrow cellularity.  相似文献   

16.
恶性肿瘤化疗后粒细胞集落刺激因子应用时间的临床探讨   总被引:4,自引:0,他引:4  
目的 探讨粒细胞集落刺激因子(G-CSF)在肿瘤化疗后适合的用药时间。方法 利用随机分组和分组对照方法。治疗组20例,化疗结束24h后即应用G-CSF,对照组20例,化疗后发现白细胞下降时使用G-CSF。结果 化疗结束24h后即应用G-CSF可有效防止白细胞(WBC)过度下降,并可使其明显上升。结论 化疖4h后即应用G-CSF,可减轻化疗后骨髓抑制和为如期化疗提供有效保障。  相似文献   

17.
The effects of acute benzene (BZ) exposure on hematopoietic progenitor cells (HPCs) derived from bone marrow cells were studied using homozygous male v-Ha-ras Tg.AC mice at 8-10 weeks of age. The mice were given 0.02% BZ in their drinking water for 28 days with the dose rate estimated to be 34 mg benzene/kg BW/day. Analysis of cultured HPCs indicated that BZ suppressed the proliferation of the multilineage colony forming unit-granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM); colony forming unit-granulocyte, macrophage (CFU-GM); and blast forming unit erythrocyte/colony forming unit erythrocyte (BFUE/CFUE). A gene expression profile was generated using nylon arrays spotted with 23 cDNAs involved in selected signal pathways involved in cell distress, inflammation, DNA damage, cell cycle arrest, and apoptosis. Of the 23 marker genes, 6 (bax, c-fos, E124, hsf1, ikBa, and p57) were significantly (Mann-Whitney U tests, P < 0.05) overexpressed in BZ-exposed mice. Two genes (c-myc and IL-2) approached significance (at P = 0.053). The pattern of gene expression was consistent with BZ toxicity and the suppression of HPCs.  相似文献   

18.
The limits of stimulation of the immunomodulatory alkaloid swainsonine (8alphabeta-indolizidine-1alpha,2alpha,8beta-triol) were studied in inbred C57BL/6 mice for potential support of intense high dose cancer chemotherapy and/or radiation because of its attractive pharmacologic profile on the hematopoietic system. Specifically, the effects of swainsonine on bone marrow cellularity and on in vitro progenitor cell proliferation to total colony forming units (CFU) and differentiation to different lineages were studied as a function of number of days post drug administration. The lineages evaluated were colony forming units-granulocyte-macrophage (CFU-GM), erythroid-burst forming units (BFU-e) and CFU-granulocyte-erythrocyte-monocyte-megakaryocyte (CFU-GEMM or CFU-Mix). Groups of mice were treated with swainsonine or plain vehicle, phosphate buffered saline for 10 consecutive days. The effects of these agents on the hematopoietic system were studied up to 60 days following their discontinuation. The magnitude of the effects of swainsonine on bone marrow system gradually declined with increasing duration of days following its discontinuation. Nevertheless, its residual stimulatory effects on bone marrow cellularity, total CFU, CFU-GM, BFU-e and CFU-Mix continued to be significant (P<0.0001) up to 45, 50, 50, 55 and 50 days, respectively, compared to those of diluent buffer or untreated controls. Since cancer chemotherapeutic agents or radiation are normally given in schedules and/or cycles, these results strongly suggest that swainsonine effects are sustained long enough to potentially support and facilitate hematopoietic recovery during anti-cancer cytotoxic treatment.  相似文献   

19.
The effect of cytotoxic drugs was tested on the hematopoietic system by assay of the ability of granulocyte-macrophage colony forming cell (GM-CFC) in mice to create GM colonies. The in vivo ability of bone marrow progenitor cells to granulocyte-macrophage colony formation, was tested after long-term peritoneal cytotoxic drug administration. The direct effect of these agents on cells in vitro culture was evaluated also. It was found that cytotoxic drugs inhibit the GM colony formation. The degree of damage to the bone marrow progenitor cells by assaying in vivo colony formation inhibition, depends on the drug dosage and length of therapy (after 25-30 days of treatment the colony growth was below 50%). The in vitro inhibition of granulocyte-macrophage colony formation depends on the concentration of drug (10(-7)-10(-5) M is critical for GM colony growth). The results suggest the possibility of the GM-CFC growth testing as an indicator of the progress or side effects of cytotoxic therapy.  相似文献   

20.
Ubenimex enhanced colony formation of bone marrow cells from CDF1 mice induced by L920 cell supernatant, which shows a macrophage-colony-stimulating activity (M-CSA), and also enhanced the colony formation induced CDF1 mouse spleen cell conditioned medium, which shows a granulocyte and macrophage-colony-stimulating activity. The maximal effect was obtained at 0.01 microgram/ml. But, ubenimex showed no effect on the nature of the colonies induced by each CSA. By preincubation of the bone marrow cells with ubenimex, M-CSA-induced colony-forming and the M-CSA-binding activities of the cells were increased. These results suggest that ubenimex enhances the CSA-induced colony formation of bone marrow progenitor cells of CDF1 mouse by increasing the amount of the CSA-binding to the cells.  相似文献   

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