首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 62 毫秒
1.
目的:研究三硝基苯磺酸(TNBS)/乙醇致溃疡性结肠炎(UC)大鼠免疫球蛋白、补体及红细胞免疫功能的变化。方法:48只大鼠随机分为对照组、模型1组、模型2组、模型3组,每组12只。模型组用0.5 ml含20 mg TNBS的30%乙醇灌肠制备UC大鼠模型,对照组用0.9%氯化钠注射液0.5 ml灌肠。检测UC大鼠血清免疫球蛋白、补体及红细胞免疫功能的变化。结果:造模后1周、3周、7周模型组大鼠外周血IgA、造模1周和3周IgG和红细胞免疫复合物含量均明显高于对照组(P0.01),在造模1周时表达最高,3周时已有下降,7周时与1周模型差异均有统计学意义(P0.01);IgM与对照组差异无统计学意义(P0.05)。而补体C3和红细胞表面C3b受体在3个时间段及造模后1周与3周C4均较对照组降低(P0.01)。结论:免疫球蛋白、补体及红细胞免疫功能的变化,说明UC大鼠存在免疫功能亢进,导致循环免疫复合物在肠黏膜沉积,进而引发肠黏膜局部变态反应。  相似文献   

2.
目的:探讨TNBS/乙醇法诱导大鼠溃疡性结肠炎模型溃疡、炎症持续时间及如何建立持续而稳定的大鼠慢性溃疡性结肠炎模型。方法:SD大鼠70只,随机分成空白组(10只)、模型A组和模型B组(各30只)。除空白组外各模型组采用TNBS/乙醇法诱导大鼠溃疡性结肠炎模型,模型A组大鼠给予一次性灌肠造模;模型B组每隔7d重复造模,持续至第8周。各模型组大鼠分别于造模第2周、5周、8周(空白组大鼠于造模第8周)随机抽取8只大鼠处死,进行结肠病变肉眼评分及结肠组织病理学评分。结果:与空白组比较,模型A组大鼠的体重、结肠病变肉眼评分及病理学评分在第2周有显著性差异(P〈0.01),在第5周、第8周无差异(P〉0.05);模型B组大鼠的体重、结肠病变肉眼评分及病理学评分在第2周、第5周、第8周均有差异或显著性差异(P〈0.05或P〈0.01)。结论:采用TNBS/乙醇法每周灌肠造模可成功构建持续而稳定的大鼠慢性溃疡性结肠炎模型。  相似文献   

3.
目的:建立符合中医证候特点的脾虚湿困型溃疡性结肠炎(ulcerativecolitis,UC)大鼠模型。方法:60只大鼠随机分为正常对照组、三硝基苯磺酸(trinitrobenzenesulfonicacid,TNBS)模型组,内外因加TNBS模型组及健脾化湿防治组。正常对照组给予普通喂养;TNBS模型组采用TNBS与乙醇复合物进行造模,内外因加TNBS模型组大鼠给予环境与饮食因素干预结合TNBS与乙醇复合物进行造模,健脾化湿防治组在内外因加TNBS模型复制过程中给予参苓白术散煎剂灌胃。观察各组大鼠症状、体征、病理改变,并检测血清白细胞介素6(interleukin-6,IL-6)、白细胞介素8(interleukin-8,IL-8)及肿瘤坏死因子a(tumornecrosisfactor-α,TNF-α)的浓度。结果:内外因加TNBS模型组大鼠出现嗜睡懒动、蜷缩,饮少纳呆,精神萎靡,毛发疏松粗糙、晦暗无光泽,腹泻及黏液血便症状;大鼠体质量明显下降,摄食饮水量明显降低,小便量明显减少,大便湿重增加,自发活动次数明显减少,与正常对照组比较差异有统计学意义(P〈0.05);镜下观察黏膜见水肿、充血、大量炎症细胞浸润,伴有溃疡灶形成;大鼠结肠组织损伤程度评分及IL-6、IL-8和TNF-α的浓度与正常对照组比较,差异有统计学意义(P〈0.01)。健脾化湿防治组大鼠经治疗后体质量明显增加,摄食饮水量明显增多,小便量明显增加,大便湿重减少,自发活动次数明显增多,与TNBS模型组及内外因加TNBS模型组比较,差异有统计学意义(P〈0.05);结肠黏膜见轻度充血、水肿,仅有少量炎性细胞浸润,其结肠组织损伤程度评分及IL-6、IL-8和TNF-α的浓度与内外因加TNBS模型组比较差异均有统计学意义(P〈0.05)。结论:环境与饮食因素干预结合TNBS与乙醇复合物建立的脾虚湿困型UC大鼠模型,符合人类自然发生的UC病变特点和中医证候特点,是较理想的脾虚湿困型UC模型。  相似文献   

4.
目的:观察侗药五味止泻汤对溃疡性结肠炎(UC)模型大鼠结肠组织学改变的作用.方法:随机将60只大鼠分为正常组、模型纽、侗药五味止泻汤低、中、高三个剂量组和美沙拉嗪组,除正常组外,其他5组均采用2,4,6-三硝基苯磺酸(TNBS)/乙醇复合灌肠法制备大鼠溃疡性结肠炎模型.中药低、中、高剂量组分别给予不同剂量铜药五味止泻汤灌胃,美沙拉嗪组予以美沙拉嗪灌胃,正常组及模型组灌服等体积蒸馏术.观察大鼠一般情况和黏膜损伤程度评分,在光镜下观察各组结肠组织的病理变化.结果:模型纽肠黏膜出现大小不等的溃疡,光镜下显示炎症和水肿程度较重.观察侗药五味止泻汤低、中、高剂量组肠黏膜炎症程度、粘膜水肿和充血程度均明显轻于模型组,且侗药五味止泻汤可使结肠黏膜损伤程度评分降低.结论:侗药五味止泻汤能显著改善溃疡性结肠炎模型大鼠结肠组织病理病变,对溃疡性结肠炎模型大鼠有较好的治疗作用.  相似文献   

5.
血府逐瘀口服液对大鼠溃疡性结肠炎的治疗作用及机理   总被引:1,自引:0,他引:1  
目的观察血府逐瘀口服液对大鼠溃疡性结肠炎(ulcerative colitis,UC)模型的治疗作用,探讨血栓前状态(prethrombosis state,PTS)在UC发病中的作用。方法用2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzenesulfonic acid,TNBS)制备大鼠UC模型,将动物模型随机分为模型组,模型 生理盐水组,模型 血府逐瘀口服液组,另设空白对照组,共4组。观察各组大鼠肠道大体形态和组织学改变;检测血小板黏附功能(platelet adhesivability,PAdT)和血小板聚集功能(platelet aggregability,PAgT);酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)测定血清中的血栓烷B2(thromboxane B2,TXB2)、血管性假血友病因子相关抗原(von Willebrand Factor antigen,vWF∶Ag)和细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)。结果与模型 生理盐水组比较,模型 血府逐瘀口服液组大体形态和组织学损伤指数明显下降(6.02±0.74 vs 0.93±0.36,P<0.01;5.68±0.60 vs 1.13±0.32,P<0.01);模型 血府逐瘀口服液组的PAdT和PAgT较模型 生理盐水组降低(P<0.05);模型 血府逐瘀口服液组的TXB2、vWF∶AgI、CAM-1均较模型 生理盐水组明显降低(P<0.01)。结论TNBS所诱导大鼠UC模型与PTS有一定关系。血府逐瘀口服液通过抗血小板聚集、抗炎症介质而发挥治疗UC作用。  相似文献   

6.
目的建立大鼠复发性结肠炎模型。方法雄性Wistar大鼠随机分为正常对照组、急性结肠炎组、自发性愈合组和复发性结肠炎组,用80mg/kg 2,4,6-三硝基苯磺酸(TNBS)50%乙醇溶液建立大鼠溃疡性结肠炎(UC)模型,在造模第16天时,用30mg/kgTNBS 50%乙醇溶液进行第2次致炎诱导复发性结肠炎,对模型进行大体和组织学观察评分,并检测病变组织中的髓过氧化物酶(MPO)活性。结果与正常对照组、急性结肠炎组和自发性愈合组比较,复发性结肠炎组大鼠大体和组织学损伤评分均明显增高(P〈0.05或P〈0.01);与正常对照组和急性结肠炎组比较,复发性结肠炎组大鼠结肠组织中MPO活力明显升高(P〈0.05或P〈0.01)。结论采用TNBS/乙醇在首次致炎两周后进行第2次致炎,可以模拟UC缓解-复发交替出现的特点,是较理想的人类UC模型,可以应用于UC缓解期复发的研究。  相似文献   

7.
目的:用三硝基苯磺酸(2,4,6-trinitrobenzenesulfonic acid,TNBS)和乙醇灌肠建立克罗恩病(Crohn disease,CD)大鼠模型,并比较不同乙醇浓度和用量对模型的影响。方法:按150mg/kg标准,分别向大鼠肠道内灌注体积比为2:1的TNBS、50%乙醇混合液(实验1组),体积比为1:1的TNBS、50%乙醇混合液(实验2组),体积比为2:1的TNBS、无水乙醇混合液(实验3组),体积比为1:1的TNBS、无水乙醇混合液(实验4组)。生理盐水对照组注入等量生理盐水,正常对照组不予任何处理。于造模后第3、7、14和21天分批处死大鼠,观察各时间点结肠大体形态改变和组织学改变。结果:实验3组大鼠造模后1周内主要表现为肠壁非连续性糜烂、坏死及溃疡,镜下见大量炎性细胞浸润;第14天时黏膜呈鹅卵石样改变;第21天时病变明显好转,溃疡部位肠壁增厚明显,但光学显微镜下仍有明显炎症反应。实验1组在第3、7天时与实验3组的表现相似,第14天取材时症状和组织学改变均较实验3组轻,但组内大鼠间个体差异较大。实验2组第14天时大部分肠壁形态基本恢复正常。实验4组死亡率过高,组织损伤严重,且病理表现与克罗恩病差异较大。结论:体积比为2:1的TNBS、无水乙醇溶液灌肠造成的模型与人类克罗恩病症状表现近似,病理表现典型,炎症持续时间较长,是一种研究克罗恩病发病机制和评价治疗效果的较好的模型制备方法。  相似文献   

8.
目的 观察痛泻要方对溃疡性结肠炎(UC)模型大鼠结肠黏膜ERK1、ERK2基因表达的影响,探讨其作用机制.方法 将实验动物分为6组,采用TNBS/乙醇灌肠法造模,肉眼观察结肠大体形态损伤并进行评分,以大鼠结肠黏膜ERK1、ERK2为观察指标,采用RT-PCR法检测ERK1、ERK2基因表达水平.结果 肉眼观察模型组大鼠结肠组织黏膜层可见炎症和溃疡形成,与空白组比较,P<0.05,证实模型成功.模型组大鼠结肠组织ERK1、ERK2基因的相对表达量均高于空白组(P<0.05);治疗后,痛泻要方高剂量组、中剂量组ERK1、ERK2基因的相对表达量均较模型组升高(P<0.05,P<0.01).结论 痛泻要方对TNBS/乙醇法UC大鼠模型结肠黏膜ERK1、ERK2基因的相对表达量有上调作用,提示痛泻要方治疗作用可能与ERK信号转导途径被激活有关.  相似文献   

9.
目的 研究4种提取工艺制备的溃结康栓对2,4,6-三硝基苯磺酸(TNBS)诱导溃疡性结肠炎(UC)大鼠的治疗作用.方法 SD雄性大鼠随机分为正常对照组、模型组、美沙拉秦栓组和4种提取工艺制备的溃结康栓组共7组,每组10只,用TNBS法复制UC大鼠模型,各给药组给予相应栓剂治疗,连续14 d.以各组实验大鼠疾病活动指数(DAI)、结肠黏膜损伤度、组织病理学改变、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-4(IL-4)等为指标,对其治疗疗效进行评价.结果 模型组大鼠DAI评分、结肠黏膜损伤评分,血清中TNF-α、IL-1β 及IL-4水平与正常对照组比较差异有统计学意义(P<0.01),美沙拉秦栓组、各工艺溃结康栓组DAI评分、结肠黏膜损伤评分显著降低,TNF-α、IL-1β 及IL-4水平与模型组比较有统计学意义(P<0.05);美沙拉秦栓组与工艺Ⅰ溃结康栓组、工艺Ⅳ溃结康栓组DAI评分,TNF-α、IL-1β 及IL-4水平比较无统计学意义.结论 4种提取工艺制备的溃结康栓均对TNBS诱导的UC模型大鼠有治疗作用,其中以提取工艺Ⅳ制备的溃结康栓疗效尤为显著,可为溃结康栓制剂研究提供工艺基础.  相似文献   

10.
目的:探讨同种异体大鼠骨髓间充质干细胞(MSCs)移植于大鼠溃疡性结肠炎(UC)模型后其在结肠的定位情况.方法:将用贴壁法体外连续传代培养的雄性SD大鼠的MSCs经尾静脉注射于雌性大鼠UC模型体内,并以UC模型组及酒精灌肠为对照组.分别于移植后第1天、第7天、第21天后取远端结肠组织,经分子生物学方法检测Y染色体的性别决定区段(sry)作为标志,以确定MSCs的肠道定位情况.结果:培养的MSCs集落生长迅速,均一性好.TNBS引起肠壁明显增厚,炎症和溃疡持续时间较长,髓过氧化物酶活性值显著性升高,不同时期均与对照组有明显差异(P<0.01),黏膜及黏膜下层有大量中性粒细胞、淋巴细胞、巨噬细胞及纤维细胞浸润.移植组的sry检测显示:第1天,第7天和第21天阳性率分别为0,16.7%(1/6)和50.0%(3/6),而对照组均阴性.结论:移植后的MSCs能在UC模型的肠道中定位.  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号