HLA-DRB1 allele in 35 patients with alveolar echinococcosis in Gansu Province of China

Objective To investigate the association between histocompatibility leukocyte antigen (HLA)-DRB1 alleles and alveolar echinococcosis (AE).Methods Thirty-five patients with AE in high prevalence areas in Gansu Province of China were tested for the HLA-DRB1 gene using the polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. The results were compared with those of 104 healthy individuals.Results The frequency of the HLA-DRB1*040x gene was 26% in the patient group, which was significantly higher than that in the control group (9.62%) with a relative risk (RR) of 4.45 (χ2 =13.67, P<0.01), and an etiological fraction (EF) of 0.20. The frequency of the HLA-DRB1*0701 allele was significantly lower in the patient group (2.86%) as compared to the control group (13.94%; χ2=6.67, P<0.05) with a preventable fraction (PF) of 0.30. The frequencies of other DRB1 alleles were not significantly different.Conclusion Susceptibility to AE is significantly associated with the HLA-DRB1*040x. HLA-DRB1*0701 gene might confer protection against AE in humans.     

Isolation of genes related to blood glucose-control in rat skeletal muscle

ｉｆｆｅｒｅｎｔｉａｌｄｉｓｐｌａｙｒｅｖｅｒｓｅｔｒａｎｓｃｒｉｐｔｉｏｎ ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ (ＤＤＲＴ ＰＣＲ )ｏｆｍＲＮＡｉｓａｒｅｃｅｎｔｌｙｄｅｖｅｌｏｐｅｄｔｅｃｈｎｉｑｕｅｗｈｉｃｈａｌｌｏｗｓｔｈｅｉｄｅｎｔｉｆｉｃａｔｉｏｎａｎｄｍｏｌｅｃｕｌａｒｃｌｏｎｉｎｇｏｆｇｅｎｅｓｄｉｆｆｅｒｅｎｔｉａｌｌｙｅｘｐｒｅｓｓｅｄａｔｔｗｏｓｔａｔｅｓｉｎａｇｉｖｅｎｔｉｓｓｕｅ 1　Ｔｈｅｄｉｓｒｕｐｔｉｏｎｏｆｗｈｏｌｅ ｂｏｄｙｇｌｕｃｏｓｅｈｏｍｅｏｓｔａ…     

Analysis of low-density lipoprotein receptor gene mutations in a Chinese patient with clinically homozygous familial hypercholesterolemia

Objective To screen the point mutation of the low-density lipoprotein receptor （LDL-R） gene in Chinese familial hypercholesterolemia (FH) patients, characterize the relationship between the genotype and the phenotype and discuss the molecular pathological mechanism of FH. Methods A patient with clinical phenotype of homozygous FH and her parents were investigated for mutations in the promoter and all eighteen exons of the LDL-R gene. Screening was carried out using Touch-down PCR and direct DNA sequencing; multiple alignment analysis by DNASIS 2.5 was used to find base alteration, and the LDL-R gene mutation database was searched to identify the alteration. In addition, the apolipoprotein B gene (apo B) was screened for known mutations (R3500Q) that cause familial defective apo B100 (FDB) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results Two new heterozygous mutations in exons 4 and 9 of the LDL-R gene were identified in the proband (C122Y and T383I) as well as her parents. Both of the mutations have not been published in the LDL-R gene mutation database. No mutation of apo B100 (R3500Q) was observed. Conclusion Two new mutations (C112Y and T383I) were found in the LDL-R gene, which may result in FH and may be particularly pathogenetic genotypes in Chinese people.     

中国强直性肌营养不良1型患者的分子诊断研究

Background Myotonic dystrophy type 1 （DM1） is an autosomal dominant multisystem disease caused by abnormalexpansion of cytosine-thymine-guanine （CTG） repeats in the myotonic dystrophy protein kinase gene. The clinicalmanifestations of DM1 are multisystemic and highly variable, and the unstable nature of CTG expansion causes widegenotypic and phenotypic presentations, which make molecular methods essential for the diagnosis. So far, very fewstudies about molecular diagnosis in Chinese patients with DM1 have been reported. Therefore, we carried out a studyusing two different methods in molecular diagnosis to verify the validity in detecting CTG expansion in Chinese patientsshowina DM sians.     

Impaired gp-91phox gene expression and dysfunction of peripheral blood neutrophils in patients maintaining hemodialysis

Ｎｅｕｔｒｏｐｈｉｌｓｐｌａｙａｎｉｍｐｏｒｔａｎｔｒｏｌｅｈｏｓｔｄｅｆｅｎｓｅｓｙｓｔｅｍａｇａｉｎｓｔｉｎｆｅｃｔｉｏｎ Ｔｈｅｎｅｕｔｒｏｐｈｉｌｓｇｒａｎｕｌｅｓｆａｌｌｉｎｔｏｐｅｒｏｘｉｄａｓｅ ｐｏｓｉｔｉｖｅｇｒａｎｕｌｅｓｄｅｆｉｎｅｄｂｙｔｈｅｉｒｃｏｎｔｅｎｔｓｏｆｍｙｅｌｏｐｅｒｏｘｉｄａｓｅ (ＭＰＯ)ａｎｄｐｅｒｏｘｉｄａｓｅ ｐｏｓｉｔｉｖｅｇｒａｎｕｌｅｓｗｈｉｃｈｍａｙｂｅｆｕｒｔｈｅｒｓｕｂｄｉｖｉｄｅｄｉｎｔｏｓｐｅｃｉｆｉｃｇｒａｎｕｌｅｓａｎｄｇｅｌａｔｉｎ…     

HLA alleles in patients with Guillain-Barre syndrome

ＧｕｉｌｌａｉｎＢａｒｒｅｓｙｎｄｒｏｍｅ(ＧＢＳ)ｉｓａｃｏｍｍｏｎｎｅｕｒｏｌｏｇｉｃａｌｄｉｓｅａｓｅｗｉｔｈｃｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆｆｌａｃｃｉｄｐａｒａｌｙｓｉｓＴｈｅｅｔｉｏｌｏｇｙｏｆＧＢＳｉｓｔｈｏｕｇｈｔｔｏｂｅｄｅｍｙｅｌｉｎａｔｉｏｎａｎｄａｘｏｎａｌｄａｍａｇｅｓａｆｔｅｒｉｎｆｅｃｔｉｏｎＴｈｅｅｘａｃｔｍｅｃｈａｎｉｓｍｉｓｓｔｉｌｌｕｎｃｌｅａｒＭｏｌｅｃｕｌａｒｍｉｍｉｃｒｙｈｙｐｏｔｈｅｓｉｓｏｆＧＢＳｉｓｔｈｅｍｏｓｔｃｉｔｅｄｅｘｐｌａｎａｔｉｏｎＴ…     

Detection of hepatitis G virus RNA in hepatitis C patients and hepatitis C virus infected hemodialysis patients

AlthoughsensitiveandspecificimmunoassayandmolecularbiologicaltechniquesforthedetectionofthehepatitisA--Evirusesareavailable,theetiologyofasubstantialfractionofpost--transfusionandcommunity--acquiredhepatitiscasesremainsundefined[1],suggestingtheexistenceofadditionalcausativeagents.Anewhumanhepatitisviruswasisolatedbytwoindependentgroups.ThenewvirusisprovisionallydesignatedashepatitisGvirus(HGV)[ZJorGBvirusC(GBV~C)[3'4),whichwasthoughttobetheagentofpartofthenon--A--Ehepatitispatients.Fro…     

丁型肝炎病毒RNA巢式逆转录PCR检测

为了提高丁型肝炎病毒的实验室诊断水平，建立一个高敏感性和特异性的丁型肝炎病毒的检测方法。根据ＧＥＮＢＡＮＫ中ＨＤＶ的核酸序列设计出巢式引物，采用逆转录巢式ＰＣＲ方法对４０例肝炎患者进行检测。结果：２０例丁型肝炎病毒抗原阳性的ＨＤＶＲＮＡ均为阳性，１０例丁型肝炎病毒抗体阳性中ＨＤＶＲＮＡ阳性６例，１０例单纯ＨＢｓＡｇ、ＨＢｅＡｇ和抗ＨＢｃ阳性的均未检出ＨＤＶＲＮＡ。结论：巢式逆转录ＰＣＲ可以作为丁型肝炎病毒临床检测的一种方法     

微孔板固相杂交法检测丙型肝炎病毒

目的 寻找一种快速、准确、简便的丙型肝炎病毒（hepatitis C virus，HCV）RNA逆转录-聚合酶链反应产物的检测方法。方法 所有标本均作逆转录-套式聚合酶链反应，其产物分别以微孔板固相杂交法及聚丙烯酰胺凝胶电泳法检测，对结果可凝及阴性者均以HCV Amplification KIT对其血清再作扩增并检测，并以此为金标准进行比较。结果 144份血清（其中抗-HCV阳性64例），微孔板固相杂交法阳性70例，灵敏度97.18%，特异度98.63%，聚丙烯酰胺凝胶电泳法阳性61例，灵敏度81.69%，特异度95.89%，取同一份产物倍比长稀释后再作检测，前者可检测出2^-9稀释度，后者检测出2^-7稀释度。结论 微孔板固相杂交法检测丙型肝炎病毒快速、准确、简便，并且可定量，尤其适用于大批量标本的检测，但于基层推广。     

Genotyping of hepatitis C virus in patients with hepatocellular carcino Ma

ＧｅｎｏｔｙｐｉｎｇｏｆｈｅｐａｔｉｔｉｓＣｖｉｒｕｓｉｎｐａｔｉｅｎｔｓｗｉｔｈｈｅｐａｔｏｃｅｌｌｕｌａｒｃａｒｃｉｎｏＭａ￥ＣｕｉＸｉａｏｈｏｎｇ（崔晓红）；ＰａｎＷｅｉ（潘卫）；ＱｉＺｈｏｎｇｔｉａｎ（戚中田）；ＳｏｎｇＹａｎｂｉｎ（宋燕斌）...     

丙型肝炎患者血清中丙型肝炎病毒交叉中和抗体的检测

[摘要]目的分析HCV感染者血清中是否存在交叉反应性中和抗体。 方法以分泌表达HCV包膜E2蛋白真核表达质粒转染的293T 细胞培养上清液中的HCV E2蛋白作为检测抗原,建立检测HCV E2抗体的ELISA方法,检测32份HCV抗体阳性的慢性丙肝患者血清,然后用免疫荧光分析血清与HCV全长包膜蛋白表达质粒转染的293T细胞的结合反应,再用5株HCV假病毒(HCVpp)及两株细胞培养产生的HCV(HCVcc)为模型分析血清的病毒中和活性。 结果32份HCV抗体阳性血清中,26份血清可检测出E2抗体,阳性率81.3%。其中HCV RNA阳性的12份血清均为E2抗体阳性,E2抗体水平与HCV RNA水平负相关。HCV E2抗体阳性血清对5株HCVpp以及两株HCVcc的感染性均有不同程度的中和作用,中和活性与E2抗体水平相平行。结论HCV感染可诱导保护性体液免疫应答,丙肝患者血清中存在交叉中和抗体,提示开发能诱导广泛交叉中和抗体的丙肝疫苗具有可行性。     

Detection of plus and minus strands of HCV RNA in cancerous and non－cancerous liver tissues of patients with hepatocellular c

ＤｅｔｅｃｔｉｏｎｏｆｐｌｕｓａｎｄｍｉｎｕｓｓｔｒａｎｄｓｏｆＨＣＶＲＮＡｉｎｃａｎｃｅｒｏｕｓａｎｄｎｏｎ－ｃａｎｃｅｒｏｕｓｌｉｖｅｒｔｉｓｓｕｅｓｏｆｐａｔｉｅｎｔｓｗｉｔｈｈｅｐａｔｏｃｅｌｌｕｌａｒｃａｒｃｉｎｏｍａＣｕｉＸｉａｏｈｏｎｇ...     

丙型肝炎病毒NS5B区基因的扩增、克隆及序列分析

目的 对丙型肝炎病毒(HCV)NSSB区基因克隆并测序，研究其变异状况。方法 从HCV RNA阳性血清中抽提病毒RNA，利用巢式RT-PCR对NS5B区基因进行扩增，将扩增获得的靶cDNA克隆至P^KPL-3a质粒载体中，并以内引物为测序引物进行序列测定分析。结果 构建了已克隆HCVNS5B区基因的重组质粒KHC5—1；序列分析表明，与HCV-BDS株同源性最高，属于主要流行于我国的HCV 1b基因亚型。结论 证实了在NS5B区，与RNA依赖的RNA聚合酶(RDRP)活性密切相关的基元结构Gly-Asp-Asp及其相邻序列的同源性非常高。