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1.
采用RP-HPLC以4'-羟基-5-氯双氯灭痛为内标,测定了经皮渗透接受液中双氯灭痛的含量。考察了流动相组成、pH值等对双氯灭痛色谱行为的影响情况。方法简便准确,回收率为98.44%~102.3%,RSD<2.4%,双氯灭痛浓度在0.5~16μg/ml范围内,线性关系良好(r=0.9999),最低检测限50ng/ml。色谱条件为:C(18)Hypersil(5μm,100mm×4.6mm)不锈钢柱,甲醇pH7.0磷酸盐缓冲液(0.05mol/L)(56:44)作流动用,检测波长280nm,进样量20μl。  相似文献   

2.
反相高效液相色谱法测定人血清中氧氟沙星的含量   总被引:2,自引:0,他引:2  
建立了人血清中氧氟沙星的反相高效液相色谱测定方法,采用乙腈沉淀蛋白后直接进样,方法简便易行,准确度高,精密度好,方法回收率>98%,日内RSD2.0~4.0%,日间RSD为2.2~4.5%,血药浓度在40~6000ng/ml范围内呈线性关系,r=0.9992,采用荧光检测器,灵敏度大大提高,血药最小检测浓度为25ng/ml。  相似文献   

3.
HPLC法同时测定人血浆中四种氟喹诺酮药物的血药浓度   总被引:11,自引:0,他引:11  
研究了用HPLC同时测定人血浆中氧氟沙星、诺氟沙星、洛美沙星和环丙沙星血药浓度的方法。采用SpherisorbC(18)色谱柱(250mm×4.6mm,5μm),以甲醇-0.008mol/L磷酸盐缓冲液-0.5mol/L四丁基溴化铵(16:75:1.4,pH2.6)为流动相,检测波长280nm,血浆样品用乙腈沉淀蛋白后进样,简便快速,回收率稳定,对氧氟沙星,诺氟沙星,洛美沙星,环丙沙星四种氟喹诺酮药物检测的线性范围分别为:0.10~5.00μg/ml;0.11~5.40μg/ml;0.13~5.00μg/ml;0.10~10.00μg/ml。其方法检测限为:20ng/ml;20ng/ml;25ng/ml及40ng/ml,回收率均高于86%。应用本法观察了健康人口服氧氟沙星和环丙沙星后的血药浓度变化。  相似文献   

4.
用HPLC法分离测定牛黄解毒片中大黄素的含量。用正交设计法考察了甲醇浓度、流动相pH值、流速、柱温以及检测波长对方法耐用性的影响。色谱柱:Spherisorb C(18)(250mm×4.6mm,5μm),流动相:甲醇-水(H3PO4调pH为3.5)78:22,检测波长UV254nm,流速1ml/min,大黄素的回收率为98.2%,相对标准偏差RSD<2%。  相似文献   

5.
高效液相色谱法测定人血浆中大黄酸含量及药动学研究   总被引:1,自引:0,他引:1  
目的:建立高效液相色谱法测定人血浆中大黄酸的含量,研究大黄酸在正常人体内的药物动力学。方法:色谱柱为SpherisorbC185μ,250mm×4.6mm;流动相为甲醇-水-冰乙酸(80∶20∶0.5,pH3.7),紫外λ/nm254nm检测。用3P87程序计算6名健康志愿者口服100mg大黄酸后的药动学参数。结果:血药浓度在0.1~10.0μg/ml范围内线性关系良好,其相关系数r=0.9994,最小检测浓度30ng/ml。回收率>91.8%;日内、日间相对标准偏差(RSD)分别<8.4%、10.3%(n=5)。主要药动学参数为t1/2=4.08±0.53h,tmax=2.24±0.63h,Cmax=11.85±5.03μg/ml,AUC0→∞=93.4±43.5μg/(h·ml)。结论:该方法简单、灵敏,并为大黄酸的进一步研究开发创造了条件  相似文献   

6.
应用离子对色谱技术,同时测定天仙子中山莨菪碱、东莨菪碱、阿托品3种有效成分。色谱条件为YWG-C_(18)柱,4.6mm×250m,灵敏度:0.04AUFS,柱温:室温,进样量:20μL。山莨菪碱、东莨菪碱、阿托品的回收率分别为98.8%,RSD=0.56%;100.4%,RSD=1.2%;101.5%,RSD=0.40%。  相似文献   

7.
高效液相色谱法测定氟氧头孢的血药浓度   总被引:1,自引:0,他引:1  
建立了测定氟氧头孢血药浓度的HPLC法。采用Hypersll C18色 柱,以甲醇-乙腈-水-磷酸-三乙胺(16:678:0.28:0.4)为流动相,流速为1.0ml/min,检测波长270nm灵敏度0.05AUFS。该法线性范围为0.25~100μg/ml(r-0.9998),回事率大于93%,日内、日间RSD小于8.0%。  相似文献   

8.
HPLC测定人血浆中罗痛定浓度及药代动力学研究   总被引:11,自引:0,他引:11       下载免费PDF全文
建立了测定人血浆中罗痛定药物浓度的高效液相色谱法。采用YWGC18色谱柱(250mm×4.6mm),以甲醇-水-三乙胺(70∶30∶0.3,v/v/v,pH6.3)为流动相,维拉帕米为内标,用紫外检测器在281nm处检测。血浆样品用正己烷-正丁醇混合液(12∶1)在碱性条件下提取。血药浓度在2.5~1000ng/ml范围内呈线性关系,r=0.9991,最小检测浓度1.5ng/ml。日内、日间RSD分别<6.9%,<8.7%。方法回收率>92.1%。应用该法研究了五名健康志愿者口服罗痛定片后的药代动力学。  相似文献   

9.
反相高效液相色谱法测定人血吗氯贝胺浓度   总被引:1,自引:0,他引:1  
以乙腈-水(30:70,V/V)为流动相,氯霉素为内标,采用反相高效液相色谱法测定吗氯贝胺浓度,血吗氯胺在碱性条件下用乙醚提取,甲醇溶液残留物,在0.04~4.0μg/ml,浓度范围内线性关系良好r=0.9996,相对平均回收率为97.0%,日内RSD(%)2.6~3.5日间RSD(%)3.7~4.2,最低检出浓度0.04μg/ml本法快速,准确,灵敏度高,专一性强,适用于吗氯贝胺临床监测及科研需  相似文献   

10.
目的:采用反相高效液相色谱法(RP-HPLC)测定大鼠血浆中替尼达普钠的含量,为其动物药代动力学研究提供方法学基础。方法:分析柱为4.0mm×200mm的HypersilBDS,C18,柱径10μm,流动相0.23‰KH2PO4∶乙腈=70∶30,含0.1%三乙胺,以H3PO4调节pH至7.6。流速1.0ml·min-1,柱温30℃,紫外检测波长为232nm,进样量10μl。结果和结论:血浆中内源性杂质不干扰替尼达普钠和内标物的测定,相对回收率大于94%,日内变异系数小于5%,日间变异系数小于10%,血浆中最低检测浓度为0.10mg·L1。该方法简便可靠。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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