首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 56 毫秒
1.

摘要:目的  评价基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)技术在快速鉴定临床少见非发酵菌中的应用价值。方法  选取2013年7月-2016年10月安徽医科大学第一附属医院检验科分离的67株临床少见非发酵菌,包括痰液分离的21株,尿液分离的13株,血液分离的9株,骨髓液分离的9株,分泌物分离的8株,腹透液分离的2株,脑脊液分离的1株,胆汁分离的1株,其他体液分离的3株。以16S核糖体RNA(16SrRNA)序列分析为金标准,比较MALDI-TOF MS与Vitek2 Compact鉴定系统鉴定的准确性。结果  67株临床少见非发酵菌中,MALDI-TOF MS将66株(98.5%)鉴定到种水平,1株(1.5%)鉴定到属水平;Vitek2 Compact鉴定系统将59株(88.1%)鉴定到种水平,8株(11.9%)未能鉴定;67株少见非发酵菌均扩增到16SrRNA目的基因片段并成功测序,67株(100.0%)都成功鉴定到种水平。以16SrRNA序列分析为金标准,MALDI- TOF MS和Vitek2 Compact鉴定系统正确鉴定到种水平准确性分别为98.5%(66/67)和88.1%(59/67)。结论  与传统的细菌生化反应方法相比,MALDI-TOF MS技术操作简便、耗时短、费用低,鉴定结果与16SrRNA序列分析的符合率高,可以提高临床对少见非发酵菌鉴定的准确率。

  相似文献   

2.
金黄金葡萄球菌肠毒素基因定研究   总被引:1,自引:0,他引:1  
对40株产1种或1种以上肠毒素的金黄色葡萄球菌(以下简称金葡菌)进行分析,结果全部金葡菌肠毒素A(SEA,12/12)、金葡菌肠毒素C(SEC,19/19)、大部分金葡菌肠毒素B(SEB,23/28)的产生不因质粒的消除而消失,说明SEA、SEC的基因位点在染色体上。有5株金葡菌因质粒消除而推动产SEB的能力,其基因位点,可能在质粒或染色体上。4株金葡菌亦均因质粒消除失去产金葡菌肠毒素D的能力,其  相似文献   

3.
目的:通过对非发酵菌群的鉴定,分析该菌在我院的临床分布特征。方法:自2012年1月-2012年11月,骨科医院住院患者的各类型标本中,用分离鉴定的方法检出非发酵菌群160株。结果:160株非发酵菌群中检出不同菌属,其中假单胞菌属132株,包括铜绿假单胞菌68株、荧光假单胞菌2株、恶臭假单胞菌12株、洋葱伯克霍尔德菌50株,嗜麦芽窄食单胞菌6株,不动杆菌属12株,产碱杆菌属5株,无色杆菌属5株。160株非发酵菌群中,来源于痰标本37株,伤口分泌物85株,尿液9株,引流液21株,胸腔积液8株。结论:临床上非发酵菌群的感染中,铜绿假单胞菌占绝大多数(42.5%),洋葱伯克霍尔德菌占(31.3%)。标本类型以伤口分泌物为主(53.2%),痰次之(23.1%)。  相似文献   

4.
从南京土壤中分离到一株吸水链霉菌,命名为NAP-05,该菌株能产生除莠霉素,并且在菌体细胞内存在另一种初步鉴定为聚醚类抗生素的物质。该菌还能分泌淀粉酶。用嵌入染料如溴化乙锭(EB)等处理该菌株,发现EB处理株大部分仍保持产抗能力,而用吖啶橙(AO)处理亲株后,大部分的处理株失去除莠霉素产生能力。用原生质体再生方法处理NAP-05,发现多数再生株的产抗能力下降,提示NAP-05菌株的除莠霉素生物合成调节可能受到染色体外因子的影响。  相似文献   

5.
目的 了解从人工感染马立克病泡疹病毒(MDV)致瘤毒株京-1株发病鸡内林巴肿瘤组织中分离鉴定到的,定位于MDV基因组IRL区BamHI-I2和L片段内的一长720bp特异性cDNA基因在大肠杆菌表达系统中的表达能力。方法 将该cNDA基因克隆进行色氨酸(Trp)启动子控制下的大肠杆菌表达质粒PATH23中,连接质粒转化受体菌DH5α。分别采用原位杂交、斑点杂交和PCR法对重组转化子进行筛选鉴定,最  相似文献   

6.
宋榴艳  陈守义  侯水平 《热带医学杂志》2011,11(6):627-628,697,738
目的 对一株从广州市食品从业人员肛拭子中分离的蔗糖发酵型沙门氏菌进行鉴定.方法 应用培养特性试验、生化分析、血清型鉴定,及16S rRNA序列分析进行鉴定.结果 该菌培养特性及生化特性符合沙门氏菌定义,但与普通沙门氏菌在蔗糖利用、硫化氢产生上存在差异.血清型鉴定为山夫登堡沙门氏菌(Salmonella enterica Subsp·entericaserovar Senftenberg),16S rRNA序列分析为肠道沙门氏菌亚种(Salmonella enterica subsp).结论 该菌为一株蔗糖发酵型、硫化氢阴性的不典型山夫登堡沙门氏菌.  相似文献   

7.
目的 应用重组和克隆庆大肠杆菌中表达葡萄球菌肠毒素B(SEB)突变体。方法 利用PCR和PCR致变技术从产SEB的标准株扩增SEB基因,与原核表达载体pTrc99A重组后引入大肠杆菌JM109。通过序列测定鉴定突变基因;用聚丙烯酰胺凝胶电泳(SDS-PAGE)和单向免疫扩散试验分别检测表达产物和表达量。结果 获得了3株重组菌NJM109、M23JM109、M150JM109。测序表明,SEB-N序  相似文献   

8.
目的:探讨应用山梨糖-伊红美蓝培养基分离肠出血性大肠埃面菌O157:H7的新途径。方法:用标准野生株肠出血性大肠埃镣菌882364,以山梨醇-麦康凯培养基(SMAC)作对照,进行细菌的分离培养及菌落计数。结果:肠出血性大肠希菌O157:H7在SEMB培养基上生长较在SMAC培养基上快。在菌落计数方面二者无显著差异。  相似文献   

9.
小型无绿藻的形态与显微结构观察   总被引:1,自引:0,他引:1  
对从1名女患者面部皮肤结节活检分离的1株无绿藻菌,从形态学的角度上对该株菌进行较全面的观察。方法通过分离培养鉴定,直接涂片镜检,扫描电镜,透射龟间对其超微结构进行观察,组织病理切片观察。  相似文献   

10.
目的:通过前列腺液的细菌学培养,了解本地区慢性细菌性前列腺炎(CBP)的病原菌及其耐药性。方法:细菌的培养分离、生化鉴定均按全国统一操作规程进行,药敏实验采用WHO推荐的Kirby-Bauerifc。结果:680份标本中,分离培养出致病菌共15个菌种481株,检出率为70.74%,以革兰氏阳性菌(G+)为主。革兰氏阳性菌(G+)共有9种441株,占91.68%,其中葡萄球菌分离出420株,占致病菌87.32%;革兰氏阴性菌(G-)有6种32株,占6.65%。结论:葡萄球菌为本地区慢性前列腺炎(CBP)的主要致病菌。丁胺卡那、呋喃妥因可作为本地区前列腺炎的首选药。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号