丹皮多糖对正常及高血糖小鼠的降血糖作用

观察了丹皮多糖对正常小鼠血糖及葡萄糖致小鼠血糖的影响。结果表明，丹皮多糖粗品１００ｍｇ／ｋｇ，２００ｍｇ／ｋｇ灌胃给药可使正常小鼠血糖显著降低，２００ｍｇ／ｋｇ，４００ｍｇ／ｋ灌胃给药对葡萄糖诱发的小鼠高血糖有显著降低作用。     

骆驼蓬总碱的毒性

目的：研究骆驼蓬总碱的毒性。方法：用骆驼蓬总硷对小鼠进行急性毒性，大鼠进行亚急性毒性实验。结果：小鼠急性毒性实验测出骆驼蓬总硷，于腹腔、静脉注射及灌胃３种给药途径１次给药的ＬＤ５０，以及可信限分别为：１４４ｍｇ／ｋｇ（１３１．７￣１５７．９ｍｇ／ｋｇ）、５６ｍｇ／ｋｇ（４８．３￣６４．９ｍｇ／ｋｇ）、２８９ｍｇ／ｋｇ（２１８．５￣３８２．３ｍｇ／ｋｇ）。在大鼠亚急性毒性实验可见３００ｍｇ／ｋｇ骆驼     

骆驼蓬总碱的毒性

目的：研究骆驼蓬总碱的毒性。方法：用骆驼蓬总硷对小鼠进行急性毒性，大鼠进行亚急性毒性实验。结果：小鼠急性毒性实验测出骆驼蓬总硷，于腹腔、静脉注射及灌胃３种给药途径１次给药的ＬＤ５０，以及可信限分别为：１４４ｍｇ／ｋｇ（１３１．７～１５７．９ｍｇ／ｋｇ）、５６ｍｇ／ｋｇ（４８．３～６４．９ｍｇ／ｋｇ）、２８９ｍｇ／ｋｇ（２１８．５～３８２．３ｍｇ／ｋｇ）。在大鼠亚急性毒性实验可见３００ｍｇ／ｋｇ骆驼蓬总碱引起肾病变和尿素氮升高，停药后肾毒性程度可降低。结论：骆驼蓬总碱毒性的靶器官是肾脏，在停药之后肾脏病变在一定程度上是可逆的。     

云芝糖肽抗肿瘤的实验研究

采用小鼠移植性肿瘤病理模型,观察云芝糖肽对Ｓ－１８０Ａ荷瘤小鼠的抑瘤作用和对生命延长率的影响。结果：ＰＳＰ２５ｍｇ／ｋｇ腹腔给药,抑瘤率为３４．８５％,抑瘤率为３４．８５％;１０００ｍｇ／ｋｇ口服给药,抑瘤率为３２．９９％;连续腹腔给药２５ｍｇ／ｋｇ,１周后腹腔接种Ｓ－１８０Ａ,生命延长率达４４．６２％。     

唐古特大黄多糖对小鼠急性肝损伤的保护作用

目的：观察唐古特大黄多糖（ＴＭＰ）对小鼠急性肝损伤的保护作用及探讨ＴＭＰ对肝损伤的保护机理。方法：小鼠６０只,随机分６组,生理盐水正常对照组,药物模型组,生理盐水模型对照组和３个给药组,每组按要求分别用ＣＣｌ４ １５６ｍｇ／ｋｇ,Ｄ－Ｇａｌ－Ｎ８００ｍｇ／ｋｇ,ＴＡＡ５００ｍｇ／ｋｇ腹腔注射（ｉｐ）,造成小鼠急性肝损伤,以ＴＭＰ１００ｍｇ／ｋｇ,２００ｍｇ／ｋｇ,４００ｍｇ／ｋｇ×５ｄ灌胃,用硫代     

氢溴酸右美沙芬对实验性咳嗽的镇咳作用

目的：研究氢溴酸右美沙芬对实验性咳嗽的镇咳作用。方法：化学品引咳法，电刺激引咳法。结果：氢溴酸右美沙芬１０ｍｇ／ｋｇ和２０ｍｇ／ｋｇ给小白鼠灌胃能有效地抑制氨水所致小鼠咳嗽的次数和延长潜伏期；５．５ｍｇ／ｋｇ和１１ｍｇ／ｋｇ给豚鼠灌胃给药对丙烯醛所致咳嗽有明显镇咳作用；３．２ｍｇ／ｋｇ和６．４ｍｇ／ｋｇ给豚鼠腹腔注射，对电刺激引起的咳嗽有明显镇咳作用。咳嗽次数减少率大于５０％，抑制率分别为５９．０２％和６３．９４％。结论：氢溴酸右美沙芬对多种实验方法引起的咳嗽有明显的镇咳作用。     

芎归滴丸表理学研究

芎归滴丸急性毒性试验，采用ＩＣＲ系小鼠，ｉｇ给药ＬＤ５０为１７１６．８８ｍｇ／ｋｇ体重，ｉｐ给药ＬＤ５０为５２６．９ｍｇ／ｋｇ体重，死亡 物的内脏肉眼未见异常改变；     

云芝糖肽抗肿瘤的实验研究

采用小鼠移植性肿瘤病理模型,观察云芝糖肽（ＰＳＰ）对Ｓ－１８０Ａ荷瘤小鼠的抑瘤作用和对生命延长率的影响。结果：ＰＳＰ２５ｍｇ／ｋｇ腹腔给药,抑瘤率为３４．８５％（Ｐ＜０．０５）;１０００ｍｇ／ｋｇ口服给药,抑瘤率为３２．９９％（Ｐ＜０．０５）;连续腹腔给药２５ｍｇ／ｋｇ,１周后腹腔接种Ｓ－１８０Ａ,生命延长率达４４．６２％。表明ＰＳＰ能有效地抑制荷瘤小鼠的肿瘤生长,可明显延长Ｓ－１８０Ａ腹水小鼠的存活期。     

绞股蓝总皂甙对小鼠脾自然杀伤细胞活性的影响

采用比色法对给予一定时间、不同剂量绞股蓝总皂甙（ＧＰ）各组小鼠脾自然杀伤细胞活性（ＮＫＣＡ）进行测定。结果显示给予绞股蓝总皂甙剂量为５０ｍｇ／ｋｇ时其自然杀伤细胞活性与对照组比较相同；当剂量为１００ｍｇ／ｋｇ时，小鼠脾自然杀伤细胞活性高于对照组与５０ｍｇ／ｋｇ组；剂量２００ｍｇ／ｋｇ时的自然杀伤细胞活性与１００ｍｇ／ｋｇ相同。提示绞股蓝总皂甙达一定剂量时具有提高小鼠脾自然杀伤细胞活性的作用，当再增加给药剂量时，其进一步促自然杀伤细胞活性作用不明显。     

槲皮素对小鼠淋巴细胞发光的影响

本文研究了槲皮素对小鼠淋巴细胞化学发光的影响，槲皮素５０ｍｇ／ｋｇ，１００ｍｇ／ｋｇ给小鼠腹腔注射，每天１次，连给６天后，测定小鼠脾淋巴细胞化学发光，结果表明，给药组小鼠淋巴细胞发光值显著高于对照组（Ｐ〈０．０１）。提示槲皮素具有提高淋巴细胞活性，增强其免疫功能的作用。     

Effects of Rebixiao granules on blood uric acid in patients with repeatedly attacking acute gouty arthritis

Objective: To observe the clinical effect of Rebixiao granule (RBXG) in treating repeatedly attacking acute gouty arthritis and through experimental study on blood uric acid to explore RBXGs therapeutic mechanism.Methods: Ninety repeatedly attacking acute gouty arthritis patients were divided into the treated group (n = 60) and control group (n = 30). The treated group was treated with RBXG, and the control group was treated with Futalin tablets (diclofenac sodium). The baseline treatment including good rest, low purine diet, sufficient water drinking and urine alkalization, etc. was then given to both groups. Hypoxanthine 600 mg/kg and niacin 100 mg/kg was applied to hyperuricemic mice by gastrogavage to establish the animal models.Results: The clinical effective rate of the treated group was 95.0% and that of the control 90.0%. Good therapeutic effects were won, insignificant difference (P>0.05)was shown between the two groups. However, the cure rate of the treated group was 26.7% while that of the control group was 10.0%, with significant difference (P<0.01) shown between them. The treated group had its blood uric acid lowered, which was significantly different (P<0.05) from that of the control group. The animal experiment indicated that all the three groups treated with different dosages of RBXG, as well as the Ash bark and Smilax glabra rhizome groups had their blood uric acid content reduced in the hyperuricemic mice.Conclusion: RBXG has a quicker initiation and better treatment effects than sole anti-inflammatory and analgesic agents on the treatment of repeatedly attacking acute gouty arthritis, showing no obvious toxic or adverse reactions and therefore good for long-term administration and likely to be a safe TCM preparation to control the symptoms and reduce the onsets of repeatedly attacking of acute gouty arthritis. The animal experiment shows that both the compound preparation and part of the single ingredients in the recipe have the function of reducing blood uric acid. However, the compound recipe has better therapeutic effects, proving to be superior to single drugs. Supported by Project of Science and Technology Commission Foundation of Jiangsu Province in 1998;     

Experimental studies on combinations of pyronaridine/primaquine versus chloroquine/primaquine.

The toxicity of combined use of blood schizontocide pyronaridine (PND) and primaquine (PQ) in mice and rats was significantly lower than that of chloroquine (CQ) plus primaquine (PQ). PND 1/2 LD50 (ca 600 mg/kg) in combination with PQ reduced the toxic action of PQ in mice, while CQ 1/2 LD50 (ca 300 mg/kg) plus PQ produced synergistic toxic effect. In animal models such as Plasmodium yoelii sporozoite infection in mice and P. cynomolgi sporozoite infection in rhesus monkeys, the tissue schizontocidal action of PQ was not affected by PND. Therefore, clinical evaluation of PND/PQ in comparison with CQ/PQ in treating vivax malaria is suggested.

     

新疆草棉花总黄酮系统药效学研究

目的: 研究草棉花瓣提取物总黄酮(FGF)的毒性、抗炎免疫等方面的药效学,为研究和开发新疆的地方药用植物奠定基础。方法: 采用二甲苯致小鼠耳廓肿胀,角叉菜胶致小白鼠足跖肿胀,棉球致大鼠肉芽肿,测定炎性组织中PGE2 和TXA2、血清溶菌酶含量、小鼠脾巨噬细胞白介素1的活性,观察FGF的抗炎作用。测定小鼠免疫器官相对重量、巨噬细胞吞噬中性红能力、血清溶血素含量,采用四甲基偶氮唑盐比色法(MTT法)测定淋巴细胞增殖反应,邻苯三酚自氧化法测SOD活性,DTNB直接法测GSH PX活性,TBA显示色法测MDA含量,观察FGF对免疫功能的影响。结果: FGF(300、600 mg/kg×7 d)对各种急、慢性炎症均有明显的抑制作用(P <0.01),FGF不同剂量组对绵羊红细胞攻击小鼠可增加脾腺指数,降低胸腺指数,呈剂量依赖性;FGF(600 mg/kg×7 d)能增加腹腔巨噬细胞的吞噬能力(P<0.01);FGF(300、600 mg/kg×7 d)可使半数溶血值(HC50)明显升高(P<0.01); FGF(300 mg/kg×l5 d)可增加IL 1活性,促进脾淋巴细胞增殖(P<0.05);FGF(150 mg/kg×l5 d)可增加心肌GSH PX、肝SOD活性,降低肾MDA含量(P相似文献   

Effects on animal models of depression of bioactive compounds from entomogenous fungi, a novel antioxidant

Objective: To study the antidepressant effects and its mechanism of bioactive compounds (metabolite extract) from entomogenous fungi (BCEF) on experimental animal models of depression. Methods: The antidepressant effect of BCEF was examined on the acquired models of depression (rats and mice in forced swimming test) and unpredictable chronic stress mouse models. The behavior alterations were assayed by detecting the duration of immobility in forced swimming test. UV spectrophotometer analysis technique was used to detect the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) in mice brain mitochondria; and colorimetric method was used to detect the content of malondealdehyde (MDA), nitrogen oxide (NO) in rat brain cytoplasm and mitochondria. Results: BCEF (25, 50, 100 mg/kg) could obviously shorten the immobility time in forced swimming mice and BCEF (50,100 mg/kg) could obviously shorten the immobility time in forced swimming rats. Both of them showed some extent     

瑞香苷对高尿酸血症小鼠的影响

目的；研究瑞香苷对高尿酸血症小鼠血清尿酸水平的影响。方法：选择化学诱导剂氧嗪酸钾盐作为尿酸酶抑制剂，小鼠腹腔注射后造成高尿酸血症模型，采用磷钼酸还原法测定小鼠血清尿酸水平。结果：腹腔注射瑞香苷（50，100和200mg/kg)能显著地减少高尿酸血症小鼠血清尿酸水平，具有一定的量效关系。瑞香苷100mg/kg剂量组作用时间可维持5h。瑞香苷在剂量为200mg／kg时可降低正常小鼠血清尿酸水平。结论：瑞香苷对高尿酸血症小鼠具有明显降低血清尿酸水平作用。     

一种拟青霉代谢物提取物对行为绝望小鼠的抗抑郁作用

目的研究一种拟青霉代谢物提取物(BCPT)对实验性行为绝望小鼠的抗抑郁作用.方法采用强迫小鼠游泳实验和小鼠尾悬吊应激实验.结果 BCPT 160、80、40、20、10 mg/kg能缩短强迫游泳小鼠的不动时间,并呈现一定的量效、时效关系, BCPT 40、20mg/kg能缩短悬尾小鼠的不动时间.结论 BCPT 在小鼠行为绝望模型实验中具有一定的抗抑郁作用.     

Evaluation of Antinociceptive and Anti-inflammatory Effects of Aqueous Extract of Armadillidium vulgare Latreille

Objective: To assess the antinociceptive and anti-inflammatory properties of the aqueous extract of Armadillidium vulgare(AV). Methods: The antinociceptive effect of AV(400, 600 and 800 mg/kg) was investigated in mice using the acetic acid-induced writhing, formalin-induced nociceptive, and hot plate tests. Phlogogen-induced paw edema using carrageenan, dextran, or compound 48/80 as phlogogen was used as inflammatory models to evaluate AV's anti-inflammatory effect. Additionally, the bioactive substances glucosamine(GLc N) and taurine in AV were determined using high-performance liquid chromatography. Results: Oral treatment of the mice with AV(600 and 800 mg/kg) significantly reduced the number of writhes in the acetic acid-induced writhing test(P0.01) but not the hot plate test(P0.05). All doses tested significantly inhibited paw-withdrawal during the second phase of the formalin-induced nociceptive model(P0.01). AV demonstrated a strong anti-inflammatory effect in all those inflammatory models(P0.05). Conclusions: AV has antinociceptive and anti-inflammatory effects, providing scientific evidence of the efficacy of its traditional use in pain treatment. Furthermore, GLc N and taurine contribute, at least in part, to the anti-inflammatory activity of AV.     

汽油添加剂甲基叔丁基醚的毒性研究

目的　通过研究汽油添加剂甲基叔丁基醚 (MTBE)对小鼠体内谷胱甘肽 (GSH)、谷胱甘肽过氧化物酶 (GSH -Px)活性的改变以及骨髓微核、精子畸变率的影响 ,了解汽油添加剂甲基叔丁基醚的毒性。方法　 40只健康昆明种成年小鼠 ,体重 3 3 .3± 6 .9g ,随机分为 4组。灌胃染毒 ,染毒剂量分别为1 6 0 0、40 0、1 0 0和 0mg kg。每天 1次 ,每周 5天 ,共计 40天。结果　MTBE染毒各组小鼠体重轻度降低 ,染毒组全血及肝组织匀浆中GSH在低剂量染毒时增高 ,而高剂量时表现为降低 ,与对照组比较差异有显著性 (P <0 .0 5 ) ;GSH -Px活性明显减低 ,染毒高剂量组与对照组比较差异有高度显著性 (P <0 .0 1 ) ;骨髓嗜多染红细胞微核及精子畸变率增高 ,与对照组比较明显增高 ,差异有显著性 (P <0 .0 5 )。结论　MTBE对昆明小鼠具有一定毒性 ,可降低全血及肝脏组织中GSH -Px活性 ,低剂量时可增加全血及肝脏组织中GSH含量 ,而高剂量可降低其含量。MTBE能使骨髓微核率、精子畸形率明显增高 ,提示MTBE具有潜在的遗传毒性。