PANI-PBOPy复合材料的制备及介电性能

采用甲基磺酸（MSA）掺杂聚苯胺（PANI）,并以MSA为溶剂,将其与聚（2,6-亚吡啶基）苯并二噁唑（PBOPy）采用溶液共混法制备 了不同PANI质量分数的PANI-PBOPy复合材料。采用红外光谱、WXRD、UV-Vis、TGA以及SEM对复合材料的结构和性能进行了表征。研究了PANI 的质量分数、温度、频率等因素对PANI-PBOPy复合材料导电性能和介电性能的影响。研究表明：当PANI的质量分数达到20%时,复合材料的电 导率增大了10个数量级;复合材料的介电常数和介电损耗则随着PANI质量分数的增加呈现先增大后减小的趋势,随着频率的升高先迅速降低而 后趋于稳定,并且随温度升高而增大,40 °C时PANI质量分数为15%的复合材料的介电常数约为230。     

碳纳米管-聚乙烯复合材料的介电性能

采用熔融共混及模压的方法制备了碳纳米管（CNT）-高密度聚乙烯（HDPE）复合材料,并用介电谱仪研究了逾渗值附近的导电填料对复合材料体系在不同温度、频率条件下的介电常数、介电损耗、交流电阻率的变化规律。结果表明：复合材料的介电常数、介电损耗均随CNT质量分数增加而逐渐增大;在频率为10³~10⁶ Hz,温度为40~130℃时,HDPE基体的介电常数随频率和温度的变化较小,而添加CNT填料的复合材料的介电常数随频率和温度的增加而略微降低。当w（CNT）<0.5%时,复合材料的交流电阻率表现出对频率的强烈依赖性;而当w（CNT）>0.5%时,在低频处表现出直流特性,在高频处显示出交流电阻率的降低。     

硅炔杂化树脂改性有机硅树脂及其复合材料的性能

用一种硅炔杂化树脂聚（甲基硅烷-二乙炔基苯）（PSP）改性有机硅树脂（HS）,通过FT-IR和TGA研究了树脂体系的固化反应及耐热性,并对制备的复合材料进行力学性能、耐热性能和介电性能研究。结果表明：当HS与PSP的质量比为5∶5时综合性能最优,树脂体系在氮气氛围下质量损失5％时的温度(Td5)为691 °C,1 000 °C时质量保留率为88%;HS-PSP树脂短切玻纤复合材料冲击强度为21 kJ/m2,弯曲强度为65 MPa,200 °C时的弯曲强度高温保留率为78%,介电常数为4.6,介电损耗因数为7.9×10-3,体积电阻为6.7×1013 Ω。改性后的复合材料具有优异的力学、耐热和介电性能。     

脐带血造血干细胞的采集、浓缩与低温冷冻保存

目的 :探讨脐带血造血干细胞库建立中的脐带血采集、分离、低温冻存的方法。方法 :自 1997年 7月至2 0 0 0年 7月已冻存经过HLA配型的脐带血 (cordblood ,CB) 3 74 4份。CB采自经检查符合要求的足月顺产或剖宫产新生儿 ,经 (citratephosphateadeninedextnse ,CPD A)抗凝 ,用密闭式血袋采集法采集。采集后置于室温保存 ,18h内处理。用羟乙基淀粉 (hydroxythylstarch ,HES)沉降后一次分离方法去除绝大多数红细胞及大部分血浆。采用以二甲亚砜 (dimethylsulfoxide ,DMSO)为主的冷冻保剂 ,冷冻保存CB。DMSO的终质量分数为 10 %。经序控降温后 ,置于 - 196℃液氮中保存。结果 :分析了 3 74 4份冻存CB ,平均采集量为 (93± 2 2 )ml(45～ 198ml)。浓缩后平均体积为 (3 8± 8)ml(3 0～ 60ml) ;浓缩前后有核细胞数 .浓缩前平均有核细胞数为 11.2× 10 8± 5 .3× 10 8(4.5× 10 8～ 4 5 .1× 10 8) ;采用HES沉降一次离心浓缩去除红细胞后的平均有核细胞数为 9.7× 10 8± 4 .6× 10 8(3 .1× 10 8～ 3 0 .5× 10 8) ;平均有核细胞回收率为 79%。质控检测 4 0份标本细胞活率为 88.2 % ,CFU GM回收率和CD3 4 +细胞计数分别为 97.6%和 86.4 %。结论 :本研究采用的CB采集、冻存及造血干细胞和病原生物检测的方法是安     

人血清蛋白与两种天然抗癌有效成分的相互作用

本文应用平衡透析法在pH7.4,振动频率为140～160次/min,25℃恒温条件下,研究了喜树碱和秋水仙碱两种天然抗癌有效成分与人血清蛋白的相互作用.实验结果表明,喜树碱和秋水仙碱均能透过Viskisg纤维素膜.秋水仙碱与人血清蛋白二者的浓度均为1×10~(-5)～5×10~(-5)mol/L时,其结合率几乎为零,而喜树碱与人血清蛋白则有较强的结合力;当喜树碱的浓度为1×10~(-5)～2×10~(-5)mol/L,人血清蛋白浓度条5×10~(-6)～1×10~(-4)mol/L时,结合常数为9.58×10~4mol~(-1),并且喜树碱在人血清蛋白分子上有唯一的结合部位,经荧光光法测知,该结合部位在人血清蛋白分子的U部位.     

马来酰肼的示波极谱法测定

在0.2mol/L LiCl 底液中,用示波极谱法一次导数或二次导数测定马来酰肼,产生一个清晰的阴极波。峰电位为-1.95伏(Vs SCE)。当马来酰肼浓度为1.0×10~(-4)～2.0×10~(-5)mol/L 和1.0×10~(-5)～4.0×10~(-6)mol/L 时(PH5.6～9.3),均与峰电流呈良好的线性关系。检出限量为2.0×10~(-6)mol/L。各种方法间的误差,其相对偏差小于0.3%。作者认为,本研究为测定马来酰肼的纯度提供了一种简单、快速、准确的分析方法。     

气隙式气敏电极的制备及性能

一种基于酸碱平衡的气隙式气敏电极,用标准玻璃磨口件及平头pH电极改制,性能优良,制作简便。用作氨电极时(以0.02mol/L NH_4Cl为中介液),测得NH_4Cl浓度在1×10~(-4)～1×10~(-1)mol/L,电极线性良好,30℃时斜率为56mV/PX;当用作二氧化碳电极时(以0.02mol/L NaHCO_3为中介液),测得NaHCO_3浓度在5×10~(-3)～5×10~(-2)mol/L,电极线性良好,级差为1.04pH。     

胰岛素对大鼠血管平滑肌细胞增殖和凋亡的影响

目的　探讨胰岛素对血管平滑肌细胞增殖和凋亡的影响 .方法　体外进行大鼠血管平滑肌细胞的培养 ,以 5～ 10代细胞为实验对象 ,随机分成空白对照组和胰岛素组 ,胰岛素组又按浓度 1× 10 - 9、1× 10 - 8、1× 10 - 7和 1× 10 - 6 mol· L- 1分为 4小组 ,在加药后 2 4 h、4 8h和 72 h用 MTT法分析细胞增殖活力 ,免疫细胞化学观察细胞核增殖抗原 (PCNA)的表达 ,用末端脱氧核苷酸转移酶介导的 d UTP缺口末端标记法检测 H2 O2 诱导的细胞凋亡 .结果　加药 4 8h后 ,1× 10 - 9mol· L- 1胰岛素组的 MMT A值 (0 .4 2± 0 .0 4 )高于空白组(0 .30± 0 .0 2 ) ,P<0 .0 5 ,低于 1× 10 - 6 mol· L- 1 胰岛素组(0 .5 2± 0 .0 5 ) ,P<0 .0 5 ;1× 10 - 9mol·L- 1胰岛素作用 4 8h后细胞的 MMT A值比空白组增加 4 0 % ,高于 2 4 h细胞的MMT A值增加率 (2 7% ) ,P<0 . 0 5 ;1× 10 - 9～ 1× 10 - 6mol· L- 1 胰岛素作用 4 8h后细胞 PCNA的表达率 (5 0 %～80 % )均高于空白对照组的 (30 % ) P<0 .0 5 ;加药 2 4 h后 ,1× 10 - 6 mol· L- 1胰岛素组细胞凋亡率 (30 % )低于空白组(70 % ) P<0 .0 5 .结论　胰岛素有明显促 VSMC(vascularsmooth m uscle cells)增殖的作用 ,并且这种作用时间和浓度依赖性 ;同时胰岛素     

低流量紧闭麻醉时安氟醚吸入浓度的探讨

目的　探讨低流量紧闭麻醉 (LFCCA)时安氟醚吸入浓度 (Fi)与氧流量、挥发罐开启浓度 (Fo)及时间 (T)的关系。方法　根据吸入麻醉药的摄取量 =吸入浓度 (% )×摄取分数 (1—Fe/Fi)×肺泡通气量(ml/min) ;肺泡通气量≌ (0 3Kg +3)× 16 0ml/min ;吸入麻醉药的摄取量 =挥发器的输出量 -排出气体所带走的麻醉药量 =氧流量×Fo -排出的气体量×Fi=氧流量×Fo - (氧流量 -氧气摄取量 )×Fi;氧气的摄取量≌(0 3Kg +3)× 10ml/min ;得出 :Fo/Fi=(3Kg +30 ) (16摄取分数 - 1)÷氧流量 +1;选择ASAⅠ～Ⅱ级择期手术需气管内全麻的患者行静吸复合麻醉 ,麻醉开始后前 10min氧流量 5L/min ,Fo=2 % ;并根据气体监测结果适当调整Fo ,使Fi=2 % ,10min后改低流量 ,并按氧流量不同随机分为四组 ,Ⅰ、Ⅱ、Ⅲ、Ⅳ组氧流量分别为0 4L/min ,0 6L/min ,0 8L/min ,1 0L/min ,并分别于麻醉开始后 10min ,30min ,…… 190min时分别监测SP ,DP ,HR、SpO2 % ,Fi,安氟醚呼出浓度 (Fe) ,MV ,PetCO2 ,调整Fo使Fi=2 % ,比较实际观测Fo和公式计算Fo。结果　实际观测Fo和公式计算Fo无显著性差异 (P >0 0 5 )。结论　Fo/Fi=(3Kg +30 ) (16摄取分数- 1)÷氧流量 +1可以指导麻醉医师在无麻醉气体监护下施行LFCCA。     

雷公藤属3种植物不同群体和个体中雷公藤甲素的研究

目的测定雷公藤属3种植物(雷公藤、昆明山海棠和黑蔓)不同群体和个体中雷公藤甲素(tripto lide),为评价雷公藤药材质量和寻找雷公藤的优质种质奠定基础。方法建立雷公藤甲素HPLC测定方法,并测定了全国主要分布区25个群体91个个体的木质部和韧皮部中的雷公藤甲素。结果黑蔓的雷公藤甲素质量分数很低;雷公藤和昆明山海棠种间的雷公藤甲素差异不明显,个体质量分数木质部为1.0×10-6~5.83×10-5;韧皮部为2.3×10-6~1.030×1-0 4。结论雷公藤和昆明山海棠不同个体雷公藤甲素质量分数最高值与最低值相差约50倍,不同居群雷公藤甲素质量分数最高值与最低值相差10多倍,不同来源的药材质量差异极大,严重影响用药的安全性;雷公藤甲素质量分数高的居群位于浙江西南部和中部、湖南新宁、贵州雷山和安徽黄山;湖南新宁、贵州雷山和浙江江山居群中有雷公藤甲素质量分数极高的个体,值得进一步研究,以寻找质量分数高的优良单株。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.