中国仓鼠V79细胞对长波紫外辐射的应激反应

用ＭＴＴ分析和克隆形成法，证明长波紫外线（ＵＶＡ）可刺激指数生长期Ｖ７９细胞增殖。效应谱在３３０－３７０ｎｍ，效应峰值位于３５０ｎｍ处。在此波长下，最侍刺激剂量为５×１０＾４Ｊ／ｍ＾２。ＵＶＡ还能诱发细胞抗药性和耐热性，并强化热休克细胞的抗药性。这些效应与照射剂量和照射与药物暴露之间时间间隔有依赖关系。     

蒿甲醚对紫外线照射后HaCaT细胞表达Fas和Bcl-2的影响

目的为探讨一定剂量不同波长紫外线照射HaCaT细胞后,不同浓度蒿甲醚对HaCaT细胞表达凋亡相关蛋白Fas和Bcl-2的影响.方法采用45.00 mJ/cm2剂量的UVB及4.00 J/cm2剂量的UVA照射培养的HaCaT细胞(永生化角质形成细胞),以羟氯喹及不同浓度的蒿甲醚进行干预处理,应用流式细胞仪检测紫外线辐照及羟氯喹和蒿甲醚处理后HaCaT细胞Fas和Bcl-2蛋白水平的变化.结果(1)与未照射组相比UVA、UVB照射均可使HaCaT细胞表达Fas增加;(2)与UV组相比UVA加羟氯喹,UVB、UVA加25μg/mL蒿甲醚组使HaCaT细胞表达Fas减少;(3)与未照射组相比UVA、UVB照射可使HaCaT细胞表达Bcl-2减少;(4)与UV组相比UVA加25μg/mL蒿甲醚组和UVB加100μg/mL蒿甲醚组可以使HaCaT细胞表达Bcl-2增加.结论小剂量(25μg/mL、50μg/mL)蒿甲醚与凋亡相关的Fas和Bcl-2的表达有关.     

白藜芦醇对UVA致HaCaT细胞氧化损伤的保护作用

目的：探讨白藜芦醇对UVA照射后HaCaT细胞的光保护作用及其可能机制。方法：采用5J／cm^2 UVA照射HaCaT细胞后，分别加入0．01mmol／L和0．1mmol／L的白藜芦醇进行干预．同时设正常对照组与UVA照射组，分别用MTT法检测细胞增殖能力，用羟胺法、比色法、TBA法检测细胞超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性及丙二醛（MDA）含量，电镜观察细胞超微结构的改变。结果：白藜芦醇能提高UVA照射后HaCaT细胞增殖能力、细胞SOD和GSH—Px活性，降低MDA含量，且呈剂量依赖性增加或降低（P〈0．05）。电镜结果显示白藜芦醇能减轻UVA对HaCaT细胞超微结构的损伤。结论：白藜芦醇能减轻UVA对HaCaT细胞增殖的抑制作用、超微结构和氧化功能的损伤，并有随剂量依赖性增加的保护作用，其机制可能与白藜芦醇提高氧化酶活性、清除自由基有关。     

长波紫外线对人皮肤成纤维细胞增殖及NO/iNOS系统的影响

目的：观察不同剂量长波紫外线(UVA)对人皮肤成纤维细胞增殖及诱导型一氧化氮合酶（iNOS) /一氧化氮(NO)系统的影响，探讨皮肤光老化机制。方法：用1，5，10 J/cm2 剂量UVA照射培养的人原代皮肤成纤维细胞。分别采用四唑盐比色实验(MTT法)、逆转录-聚合酶链反应(RT-PCR)、Western印迹以及Griess反应等技术检测UVA照射后培养不同时间（24，48和72 h）人皮肤成纤维细胞增殖情况、iNOS mRNA和蛋白表达水平及NO生成量。结果：正常人成纤维细胞在72 h之内随培养时间延长细胞存活率增加， 仅检测到低水平iNOS表达和NO生成，但在5和10 J/cm2剂量UVA照射后各时间点成纤维细胞存活数下降，iNOS mRNA/蛋白表达水平和NO生成量随着UVA剂量的增加升高，与同一时间点对照组和1 J/cm2UVA照射组相比，差异有统计学意义（P<0.01或P<0.05）,而且以各剂量UVA照射后24 h时间点细胞存活数下降、iNOS mRNA/蛋白表达水平和NO生成量增高最为明显，与同剂量UVA照射后48 h和72 h组相比，差异有统计学意义（P<0.01)。结论：UVA抑制人皮肤成纤维细胞的增殖可能与其诱导iNOS基因的表达和NO的分泌有关。     

补骨脂素加长波紫外线对NB4,K562白血病细胞株的作用

目的:探讨补骨脂素(PSO)加长波紫外线(UVA)对NB4,K562白血病细胞株的作用. 方法:以NB4,K562细胞株为研究对象,观察中药补骨脂中提取的PSO加波长为360 nm的UVA对人白血病细胞株的作用;采用析因设计,观察不同浓度的PSO、不同照射时间的UVA对不同白血病细胞株抑制率、凋亡率的影响以及各因素间的交互作用.结果:①当PSO浓度为80 mg/L,UVA照射时间为15 min时,NB4细胞的抑制率达峰值;当PSO浓度为80 mg/L,UVA照射时间为10 min时,K562细胞的抑制率达峰值.②当PSO浓度为40 mg/L,UVA照射时间为10 min 时,NB4细胞的凋亡率达峰值;当PSO浓度为80 mg/L,UVA照射时间为15 min时,K562细胞的凋亡率达峰值. ③不同UVA照射时间、不同PSO浓度对不同白血病细胞株的抑制率、凋亡率的差异有统计学意义,且各因素之间有交互作用.结论:①PSO加UVA (PUVA)可抑制NB4,K562白血病细胞株的生长,并可诱导其凋亡.②40～80 mg/L PSO与波长为360 nm的UVA照射10～15 min是PUVA抗NB4,K562白血病细胞的最佳作用点.     

表没食子儿茶素没食子酸酯影响慢性紫外线辐射诱导的人皮肤成纤维细胞凋亡和突变的研究

目的 研究紫外线(UV)照射对人皮肤成纤维细胞的细胞凋亡率、细胞周期变化和次黄嘌呤磷酸核糖转移酶(HPRT)基因位点突变频率的影响以及表没食子儿茶素没食子酸酯(EGCG)的干预作用.方法 采用一定剂量的中、长波紫外线(UVA和UVB)慢性照射培养的新生儿包皮成纤维细胞.通过流式细胞仪检测细胞凋亡率和细胞周期变化,HPRT突变分析法检测突变频率.结果 慢性UVA组引起的细胞凋亡率低于UVB组(P<0.05),而HPRT基因位点突变的频率是UVB组的4.79倍.EGCG干预组与单纯UV辐射组相比,成纤维细胞的凋亡率增加,HPRT基因位点突变的频率降低.结论 慢性照射日常剂量UVA,其损伤性高于UVB.EGCG可以升高慢性UV照射引起的细胞凋亡率,降低UV照射引起的突变频率,提示EGCG可以诱导不可逆损伤细胞的凋亡,从而减少突变细胞.     

枸杞多糖减轻长波紫外线辐射致人皮肤成纤维细胞损伤的超微结构观察

目的观察枸杞多糖（lycium barbarum polysaccharides,LBP）对长波紫外线（UVA）辐射损伤体外培养人皮肤成纤维细胞超微结构的影响。方法以体外培养的原代人皮肤成纤维细胞为实验材料,采用总剂量为2.4 J/cm2的UVA照射细胞,实验分为对照组、紫外线照射模型组和三个不同浓度LBP保护组（0.2mg.mL-1LBP＋UVA,0.4mg.mL-1 LBP＋UVA,UVA＋0.8mg.mL-1 LBP＋UVA）,应用透射电镜观察各组细胞超微结构的变化。结果 UVA辐照后细胞超微结构发生明显的变化,主要表现为核膜不连续,核内出现团块状染色质,胞浆内线粒体肿胀,空化明显,嵴断裂、溶解,粗面内质网扩张。枸杞多糖预处理可以减轻对线粒体,内质网等细胞器的损伤;且随LBP剂量的升高,辐射损伤程度逐渐减轻。结论 UVA照射可使体外培养的人皮肤成纤维细胞超微结构改变,LBP可以减轻UVA所致人皮肤成纤维细胞的损伤。     

EGCG对中长波紫外线引起的皮肤成纤维细胞光老化及光突变的干预作用

目的观察绿茶提取物中的主要活性成分没食子儿茶素没食子酸脂（EGCG）对经多次长、中波紫外线（UVA、UVB）照射后人皮肤成纤维细胞（HSF）光老化及突变情况的影响。方法分离并培养新生儿包皮HSF,将其分为正常对照组、EGCG干预组、UVA组、UVA＋EGCG组、UVB组、UVB＋EGCG组;UVB照射剂量为30mJ／cm^2,UVA照射剂量为10J／cm^2,每天照射HSF共持续2周。预实验选定EGCG浓度25μg／ml。采用组织化学染色法检测细胞中衰老相关β-半乳糖苷酶的表达量,观察细胞老化情况;采用克隆法检测次黄嘌呤磷酸核糖转移酶（HPRT）基因位点突变频率,观察紫外线照射的致突变力以及加入EGCG干预后的情况。结果（1）正常对照组及EGCG组均只见少量的β-半乳糖苷酶阳性细胞。其他几组阳性细胞比率为UVB组：43％±4％;UVA组：54％±4％;EGCG＋UVB组：64％±5％;EGCG＋UVA组：75％±5％,4组细胞间阳性比率差异有统计学意义（P〈0．05）。（2）正常对照组与EGCG处理组的HPRT自发突变率很低。单纯UVB及UVA照射诱导的突变分别是自发突变的72倍及241倍,而UVB＋EGCG组和UVA＋EGCG组分别较单纯UVB组和UVA组降低了52．78％和32．37％,差异有统计学意义（t=2．0742、2．7042,均P〈0．05）。结论UVA、UVB多次照射后均可上调HSF老化概率,加入EGCG可进一步上调其老化概率。UVA、UVB多次照射后均可增加HSF的HPRT基因位点突变频率,加入EGCG可显著减少HPRT基因位点突变频率。提示EGCG对UVA、UVB多次照射HSF后的保护干预作用可能与诱导突变细胞老化从而减少细胞突变频率有关。     

低剂量照射的淋巴细胞外液对淋巴细胞亚群功能的影响

应用单克隆抗体，通过Ｐａｎｎｉｎｇ直接法分离人外周血淋巴细胞亚群，研究了不同低剂量照射的淋巴细胞外液对ＣＤ４，ＣＤ８细胞的刺激效应，结果表明，照射剂量分别为０．５～８ｃＧｙ，１～４０Ｇｙ的淋巴细胞外液能显著刺激ＣＤ４ＣＤ８细胞ＤＮＡ合成增多，两种细胞均在４ｃＧｙ剂量点表现最强的免疫增强效应。     

PTEN在电离辐射诱导旁效应细胞中的表达变化

目的:研究PTEN基因在旁效应细胞中的表达变化。方法:采用未照射细胞与直接照射细胞按11共同培养,以及将直接照射细胞培养液离心加入未照射细胞中培养形成2种旁效应细胞模型,对比PTEN mRNA及蛋白表达在2种旁效应细胞、直接照射细胞、未照射细胞中的变化。结果:直接照射的PTEN 蛋白表达与照射剂量有关,照射剂量越大其下调越为明显;PTEN蛋白表达下调程度与细胞的处理方式有关,以受照细胞培养液培养的旁效应细胞表达下调最为显著,且2种旁效应细胞中PTEN蛋白的表达下调程度均高于直接受照细胞(P<0.01)。结论:PTEN蛋白表达与低剂量电离辐射生物学效应及电离辐射旁效应的发生发展密切相关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.