酸损伤对兔食管下括约肌形态及功能的影响

目的：观察酸灌注对兔食管下括约肌（ＬＥＳ）形态及功能的影响。方法采用０．１ｍｏｌ．Ｌ＾－１ＨＣＬ（１ｍＬ．ｍｉｎ＾－１３０ｍｉｎ．ｄ＾－１ｆｏｒ４ｄ）连续行兔食管灌注,测定ＬＥＳ的压力变化。以Ｆｌｕｏ－３作为钙指示剂,用激光共聚焦显微镜测定酸灌注前后细胞内游离钙的浓度变化,以及其对乙酰胆碱作用（Ａｃｈ）的不同反应。结果酸灌注后ＬＥＳ产生明显的炎症改变,ＬＥＳ压力降低,ＬＥＳ平滑肌内游离钙的浓度」Ｃ     

兔实验性食管炎食管平滑肌细胞内钙离子浓度的变化

目的：观察兔急性实验性反应性食管炎与正常兔食管体部平滑骨细胞内钙离子浓度的变化。方法 ２０只成年新西兰大白兔随机分成两组,一组用０．２ｍｏｌ／Ｌ ＨＣｌ在距食管下括约机（ＬＥＳ）上方６ｃｍ处连续灌注兔食管４ｄ,１ｍＬ／ｍｉｎ,３０ｍｉｎ／ｄ。     

先天性上消化道梗阻患儿手术前后胃食管测压研究

对４６例光天性肥厚性幽门狭窄（ＣＨＰＳ）和１８例先天性十二指肠梗阻患儿进行胃、食管测压，以探讨上消化道梗阻对食管下端括约肌（ＬＥＳ）功能的影响，结果：两者术前均有ＬＥＳ功能损害，十二指肠梗阻患儿改变更明显；术后７～１０ｄ均有明显恢复，但十二指肠梗阻患儿仍未完全恢复正常。同时，还观察到ＬＥＳ功能损害与发病年龄、病史及呕吐症状轻重有关。将十二指肠梗阻患儿与ＣＨＰＳ患儿的测压结果进行比较可见：前者ＬＥＳ压力明显低于后者，ＬＥＳ高压区长度在十二指肠梗阻时升段和降段长度均有改变，而ＣＨＰＳ患儿只是升段有改变，使ＬＥＳ有效长度缩短，提示胆汁与胃酸混合后比单独胃内容物对食管损害严重。     

正常新生儿,婴幼儿胃及食管压力测定

对１８０例正常新生儿、婴幼儿进行胃、食管测压，结果得出不同年龄组胃、食管内压及食管、食管上、下端括约肌（ＵＥＳ，ＬＥＳ）高压区长度的正常值，同时发现随着年龄增长，ＬＥＳ、ＵＥＳ压力逐渐降低，而食管及ＬＥＳ、ＵＥＳ高压区长度逐渐延长，在１～２月ＬＥＳ的长度较食管的长度增长明显快。ＬＥＳ压力动态观察在２个月以内的小儿压力波形不稳定，出现明显上下波动，２个月后开始平稳；胃食管返流诱发试验有３例２～３天新生儿出现阳性，占２个月以内的８．３％，２个月以上３０例小儿中无阳性；ＬＥＳ长度在１～２月之间增长最快。这些提示ＬＥＳ功能成熟在生后２个月。     

先天性食管裂孔疝患儿手术前后胃食管测压研究

对１５例先天性食管裂孔疝和１５例单纯性胃食管返流（ＧＥＲ）的患儿进行了胃、食管测压研究。结果：先天性食管裂孔疝合并ＧＥＲ的患儿食管下端括约肌（ＬＥＳ）压力及其与胃压差明显低于正常（Ｐ＜０．０５），术后仍有下降趋势。ＬＥＳ长度术后较术前延长。未合并ＣＥＲ的患儿术前ＬＥＳ压力与胃压差稍低于正常，术后压力明显下降，与正常组比较差异显著（Ｐ＜０．０５）。单纯性ＧＥＲ组ＬＥＳ压力及其与胃压差明显低于正常，而且年龄越小降低越明显，ＧＥＲ诱发试验８０％出现阳性。提示食管裂孔疝所致的解剖因素改变并不是发生ＧＥＲ的主要原因，ＬＥＳ压力也并不是衡量ＬＥＳ机能的唯一指标，对ＧＥＲ患儿的测压诊断在结合ＬＥＳ压力，长度和ＧＥＲ诱发试验进行综合分析。     

新生儿胃食管反流

胃食管反流（ｇａｓｔｒｏｅｓｏｐｈａｇｅａｌｒｅｆｌｕｘ,ＧＥＲ）是指由于全身和局部原因引起下端食管括约肌（ＬＥＳ）功能不全,胃动力紊乱,排空延迟,而致胃或十二指肠内容物反流入食管的一种临床表现,易发生新生儿期,尤其是早产儿更多见。发病率高达８０‰～...     

反流性食管炎的诊断与治疗

反流性食管炎的诊断与治疗冉宗学湖北医科大学附属第一医院（４３００６０）反流性食管炎系指胃和（或）十二指肠内容物反流入食管引起的食管粘膜炎症，主要发生在食管下端括约肌（ＬＥＳ）功能障碍时。临床表现有反胃、烧灼感，严重时可出现食管溃疡、出血、狭窄或吞咽困...     

N－单甲基左旋精氨酸对犬冠脉内皮依赖性舒张功能及内皮素－1含量的影响

作者在开胸麻醉犬心脏上，观察冠脉内灌注Ｎ－单甲基左旋精氨酸（Ｌ－ＮＭＭＡ）５ｍｇ／ｋｇ，前后冠脉血流动力学、冠脉血流储备（以阻断冠脉１５ｓ再灌注所致的反应性充血血流峰值表示）以及冠脉对不同浓度乙酰胆硷（Ａｃｈ）反应的变化，同时用放射免疫法（ＲＩＡ）测定冠脉前降支（ＬＡＤ）伴行静脉血中的内皮素－１（ＥＴ－１）含量．结果证明，Ｌ－ＮＭＭＡ灌注后心率下降，基础冠脉血流量（ＣＢＦ）下降（χ±ｓ，从２７±６ｍｌ／ｍｉｎ下降至２０±８ｍｌ／ｍｉｎ，Ｐ＜０．０５），冠脉储备下降（χ±ｓ，从９１±１９ｍｌ／ｍｉｎ下降至５０±１０ｍｌ／ｍｉｎ，Ｐ＜０．０１），平均主动脉压升高，ＥＴ－１含量明显升高（χ±ｓ，从６．５±１．０ｎｇ／Ｌ升至１５．５±３．０ｎｇ／Ｌ，Ｐ＜０．０１），Ａｃｈ引起的ＣＢＦ增加减弱（Ｐ＜０．０１）．实验结果提示，生理条件下犬冠脉一氧化氮（ＮＯ）形成对ＣＢＦ，冠脉储备有重要调节作用，同时可抑制冠脉ＥＴ－１释放．     

布比卡因对离体大鼠心脏功能和心肌能量代谢的影响

目的 研究布比卡因对离体大鼠疏脏功能和心肌能量代谢的影响。方法 Ｌａｎｇｅｎｄｒｏｆｆ心脏灌注模型。２４只ＳＤ大鼠随机分为对照组、６μｍｏｌ／Ｌ布比卡因组（Ｂ１组）和１２μｍｏｌ／Ｌ布比卡因（Ｂ２组）,每组８只。Ｂ１、Ｂ２组在平衡灌注３０ｍｉｎ后,分别续灌含６μｍｏｌ／Ｌ、１２μｍｏｌ／Ｌ布比卡因的Ｋ－Ｈ液,记录上述各组在平衡末、用药５ｍｉｎ、１０ｍｉｎ、１５ｍｉｎ、２５ｍｉｎ时的ＨＲ、ＬＶＤＰ、     

兔眼虹膜睫状体促乙酰胆硷分泌的突触前5—HT受体的研究

目的：确定离体灌注状态下,兔眼虹膜睫状体的突触前５－ＨＴ受体对３Ｈ－乙酰胆硷释放的调节作用．方法：给体外灌注培养虹膜睫状体加入３Ｈ－胆硷,电刺激四次（Ｓ１,Ｓ２,Ｓ３,Ｓ４）,刺激强度为３Ｈｚ,５０ｍＡ,１ｍｉｎ．在Ｓ２,Ｓ３,Ｓ４前加入试剂．电刺激诱导的３Ｈ－乙酰胆硷释放的水平通过计算（Ｓｘ／Ｓ１）刺激比率来确定．离子交换色谱法分离灌注液样品中３Ｈ－乙酰胆硷．液内计数仪测定标本中３Ｈ－乙酰胆硷的含量．结果：５－ＨＴ（１ｎｍｏｌ／Ｌ～１０ｍｏｌ／Ｌ）诱导的虹膜睫状体３Ｈ－乙酰胆硷释放的剂量反应曲线呈浓度依赖性（ＥＣ５０＝５８ｎｍｏｌ／Ｌ）．浓度为１ｍｏｌ／Ｌ时,５－ＨＴ诱导的３Ｈ－Ａｃｈ释放量最大,增加了（４５．１４±７．４０）％（ｎ＝６）．给组织灌注５－ＨＴ３拮抗剂Ｔｒｏｐｉｓｅｔｒｏｎ（１ｎｍｏｌ／Ｌ）及５－ＨＴ１拮抗剂Ｍｅｔｈｉｏｔｈｅｐｉｎ（０．１μｍｏｌ／Ｌ）,５－ＨＴ（１μｍｏｌ／Ｌ）诱导的３Ｈ－Ａｃｈ释放分别为对照组的８９．９６％及８８．７８％;而加入非选择性５－ＨＴ拮抗剂Ｍｉ－ａｎｓｅｒｉｎ（１０μｍｏｌ／Ｌ）及５－ＨＴ４拮抗剂ＳＤＺ－２０５５５７（０．１μｍｏｌ／Ｌ）时,５－ＨＴ（１μｍｏ     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.