毛茛总苷对垂体后叶素所致实验性心肌缺血的影响

目的观察毛茛总苷对垂体后叶素所致大鼠、小鼠实验性心肌缺血的影响及对小鼠耐缺氧实验的影响。方法采用大鼠舌下静脉注射垂体后叶素(pituitrin,Pit)制备大鼠急性心肌缺血模型,观察毛茛总苷对模型大鼠心电图T波及心率的影响;采用小鼠尾静脉注射垂体后叶素制备小鼠心肌缺血模型,观察毛茛总苷对其心电图T波及心率的影响;并观察毛茛总苷对小鼠耐缺氧实验的影响。结果毛茛总苷对大鼠和小鼠心肌缺血模型心电图T波和心率有明显改善作用;能明显延长小鼠耐缺氧存活时间。结论毛茛总苷对垂体后叶素所致的大鼠、小鼠实验性心肌缺血有保护作用,并能提高小鼠对缺氧的耐受能力。     

参桂胶囊处方对急性心肌缺血大鼠心肌超微结构的影响

目的:观察参桂胶囊有效成分处方对舌下静脉注射垂体后叶素诱发大鼠急性心肌缺血的心电图变化以及对大鼠心脏病理组织的影响.方法:采用大鼠舌下静脉注射垂体后叶素(0.35 U/kg)造成急性心肌缺血模型,观察给药组注射垂体后叶素后不同时间点Ⅱ导联心电图T波变化以及大鼠心脏病理组织切片的变化.结果:参桂胶囊有效成分处方能明显对抗...     

参桂胶囊再优化处方对大鼠急性心肌缺血的保护作用

目的:观察参桂胶囊有效成份处方对舌下静脉注射垂体后叶素(pituitrin,PIT)诱发大鼠急性心肌缺血的影响.方法:采用大鼠舌下静脉注射垂体后叶素(0.35 U/kg)造成急性心肌缺血模型,观察各给药组注射垂体后叶素后不同时间点第Ⅱ导联心电图T波变化以及血清乳酸脱氢酶(LDH)、肌酸激酶(CK)含量的变化.结果:参桂胶囊有效成份处方能明显对抗垂体后叶素引起的急性心肌缺血的心电图变化,抑制大鼠血清LDH、CK升高.结论:参桂胶囊有效成份处方组具有显著对抗垂体后叶素致大鼠急性心肌缺血的作用.     

青藤碱对大鼠急性心肌缺血性损伤的保护作用

本文采用静脉注射垂体后叶素造成大鼠急性心肌缺血模型研究青藤碱对心肌缺血性损伤的影响。青藤碱能降低垂体后叶素所引起的S-T段、T波抬高,但不影响心率。实验结果提示,青藤碱对垂体后叶素引起的急性心肌缺血有拮抗作用。     

瑞香素对垂体后叶素致大鼠急性心肌缺血保护作用的研究

目的：观察瑞香素对垂体后叶素致大鼠急性心肌缺血的保护作用。方法：采用大鼠舌下静脉注射垂体后叶素造成急性心肌缺血模型，心电图检测观察注射垂体后叶素后不同时间点的第Ⅱ导联心电图ST波变化百分率（%），测定血清肌酸激酶同工酶（CK-MB）、超氧化物歧化酶（SOD）和丙二醛（MDA）的含量，并观察各项指标的变化。结果：瑞香素能明显对抗垂体后叶素引起的急性心肌缺血的心电图变化，提高血清SOD活性、降低CK-MB和MDA含量。结论：瑞香素具有显著对抗垂体后叶素致大鼠急性心肌缺血的作用。     

参桂胶囊对急性心肌缺血大鼠心肌蛋白质组学的影响

目的:观察参桂胶囊对舌下静脉注射垂体后叶素(pituitrin,PIT)诱发大鼠心肌缺血的心电图及心肌蛋白质组谱的影响。方法:采用大鼠舌下静脉注射垂体后叶素造成急性心肌缺血模型,观察各组注射垂体后叶素后不同时间点第Ⅱ导联心电图T波变化;采用双向凝胶电泳(two-dimensional protein electrophoresis,2DE)及计算机图像分析系统对心肌总蛋白进行分离分析。结果:参桂胶囊能够明显抑制垂体后叶素引起的心电图T波的改变,各组间蛋白质斑点发生显著性变化,主要表现为蛋白点的缺失、增加或者表达量的改变。结论:参桂胶囊能明显改善垂体后叶素引起的大鼠心肌缺血症状;缺血后,大鼠心肌蛋白表达谱发生改变,发生改变的蛋白质可能与心肌组织的损伤与保护有关,而参桂胶囊可使这种改变减轻。     

神经网络分析香丹注射液抗心肌缺血有效成分的谱效相关性研究

目的 寻找香丹注射液中抗垂体后叶素致大鼠心肌缺血的主要有效成分。方法 采取大鼠舌下 iv 垂体后叶素 (0.5 U/kg) 复制大鼠急性心肌缺血模型,观察香丹注射液的不同萃取部位及萃取部位的组合对大鼠注射垂体后叶素后不同时间点Ⅱ导联心电图T波变化情况,结合香丹注射液不同极性萃取部位及萃取部位组合的指纹图谱,运用神经网络分析抗垂体后叶素致心肌缺血的香丹注射液中主要有效成分。结果 香丹注射液中主要成分对垂体后叶素引起的心肌缺血模型T波抬高存在影响,丹酚酸D和丹酚酸A在 0、5、10 s 对 T 波抬高的抑制作用随质量分数的提高而增强,其余成分在 5 s、10 s、3 min 对 T 波抬高主要为促进作用。结论 香丹注射液抗垂体后叶素致心肌缺血的主要有效成分为丹酚酸A和丹酚酸D。     

复方龙脉宁不同配比药效学实验研究

目的:通过L9(34)的正交试验设计对复方龙脉宁处方中各味药材提取物进行不同配比,探讨复方龙脉宁对大鼠心肌缺血模型心电图的影响;对寒凝血瘀大鼠模型血液流变学指标的影响。方法:采用尾静脉注射垂体后叶素造大鼠急性心肌缺血模型,用心电图机记录大鼠心电图,比较各组大鼠给垂体后叶素前后心电图T波高度变化情况;另建立寒凝血瘀大鼠模型,肝素抗凝测血液流变学指标。结果:复方龙脉宁配比组3能明显抑制垂体后叶素致心肌缺血大鼠心电图T波高度变化以及显著降低寒凝血瘀大鼠的全血黏度、血浆黏度、全血还原黏度、红细胞压积、红细胞聚集指数、红细胞变形指数。结论:复方龙脉宁配比组3对急性心肌缺血大鼠可能具有预防性及治疗性保护作用;对大鼠寒凝血瘀模型能明显改善血液流变学指标。     

蒺藜皂苷对大鼠实验性急性心肌缺血的保护作用

目的：观察注射用蒺藜皂苷（GSTT）对垂体后叶素和异丙肾上腺素致大鼠急性心肌缺血模型的影响。方法：分别采用舌下静脉注射垂体后叶素和腹腔注射异丙肾上腺素制备二种大鼠急性心肌缺血模型,观察GSTT对垂体后叶素致急性心肌缺血模型大鼠心电图异常改变和心率减慢的影响及异丙肾上腺素致心肌缺血模型大鼠血清肌酸磷酸激酶（CPK）和乳酸脱氢酶（LDH）的影响。结果：与模型组比较,GSTT可减轻垂体后叶素引起的心率减慢程度(P<0.01 ,P<0.05)和S-T段抬高程度(P<0.05),GSTT高剂量组可明显降低异丙肾上腺素致急性心肌缺血大鼠血清CPK和LDH活性(P<0.01)。结论：GSTT对大鼠实验性急性心肌缺血有保护作用。     

金丝桃苷对心肌缺血损伤的保护作用

摘要目的观察金丝桃苷对抗心肌缺血损伤的保护作用。方法观察金丝桃苷对冠状动脉结扎造成的急性心肌梗塞大鼠的梗塞面积以及对损伤大鼠血清中CPK、LDH和血清游离脂肪酸水平的影响;对用心电图ECG（LⅡ）记录注射垂体后叶素的大鼠心电图T波升高的幅度变化值;以及对异丙肾上腺素所致的大鼠心肌缺血的影响。结果金丝桃苷（12.5、25 mg*kg-1*d-1×3,ip）降低冠状动脉结扎造成的急性心肌梗塞大鼠梗塞面积,抑制血清中CPK、LDH的升高和降低血清游离脂肪酸水平;对注射垂体后叶素的大鼠心电图T波升高有抑制作用;对异丙肾上腺素所致的大鼠心肌缺血模型动物可以抑制心肌组织中CPK释放,减少心肌组织中MDA生成和提高心肌组织中SOD的含量。结论实验结果表明金丝桃苷对心肌缺血损伤有一定的保护作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.