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1.
陈盼  赵明  蒋鹏  王胜军  谷腾飞 《重庆医学》2012,41(16):1604-1606,1673
目的研究盐酸右美托咪定对大鼠心肌缺血-再灌注损伤时心肌凋亡相关基因Bax和Bcl-2表达的影响。方法将21只健康SD大鼠随机分为3组:假手术组(sham组,n=7)、缺血-再灌注组(I/R组,n=7)、缺血-再灌注+盐酸右美托咪定组(DEX组,n=7)。采用结扎左冠状动脉前降支的方法制备在体大鼠心肌缺血-再灌注损伤模型。采用RT-PCR、免疫组化方法检测Bcl-2和Bax mRNA、蛋白的表达,TUNEL法检测凋亡细胞TTC染色测定心肌梗死面积。结果与sham组比较,I/R组Bcl-2和Bax mRNA和蛋白表达增加(P<0.05);与I/R组比较,DEX组Bcl-2mRNA和蛋白表达升高(P<0.05),Bax mRNA和蛋白表达下降(P<0.05),凋亡细胞明显减少(P<0.01),心肌梗死面积减小(P<0.05)。结论盐酸右美托咪定预处理可上调心肌缺血-再灌注损伤大鼠心肌Bcl-2mRNA和蛋白表达,下调Bax mRNA和蛋白表达,表明盐酸右美托咪定在心肌缺血-再灌注损伤中有抗凋亡的作用。  相似文献   

2.
目的探讨细胞凋亡相关基因与肠缺血再灌注肺损伤的关系及七叶皂苷钠对其的影响,并探讨其作用机制。方法采用夹闭肠系膜上动脉方法复制大鼠肠缺血再灌注损伤模型。实验分为3组(n=8):对照组(Sham组)、肠缺血再灌注组(I/R组)、七叶皂苷钠组(SA+I/R组)。比较各组大鼠肺组织湿/干(W/D)比、肺系数以及血浆和肺组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量的变化;同时免疫组化法检测各组肺组织中Bcl-2和Bax蛋白的表达水平。结果与Sham组相比,I/R组肺组织W/D、肺系数以及血浆和肺组织中MDA含量升高,而SOD活性降低;同时肺组织Bcl-2和Bax的蛋白表达明显增强,且Bax的增强比Bcl-2的增强更为明显,Bcl-2/Bax比值降低;与I/R组比较,SA+I/R组肺组织W/D、肺系数以及血浆和肺组织中MDA含量降低,而SOD活性升高;同时肺组织Bax蛋白表达下降,Bcl-2蛋白表达升高,Bcl-2/Bax比值明显升高。结论肠缺血再灌注肺损伤的发生可能与氧化损伤所致的凋亡调控基因Bcl-2和Bax的蛋白表达异常密切相关,七叶皂苷钠可通过抑制过氧化损伤,上调抑凋亡基因Bcl-2的表达,提高Bcl-2/Bax比值来抑制细胞凋亡,从而减轻肠缺血再灌注肺损伤。  相似文献   

3.
目的 基于miR-503-5p/Bcl-2通路介导的细胞凋亡机制,探究清热化瘀方对脑缺血再灌注(ischemia/reperfusion,I/R)大鼠脑组织损伤的保护效应并探讨其作用机制。方法 SD大鼠分为正常组、假手术组、模型组、清热化瘀方组。线栓法建立脑I/R大鼠模型,采用清热化瘀方剂灌胃治疗。采用RT-qPCR、Western blot法分别检测缺血脑组织中miR-503-5p及Bcl-2、Bax、caspase-3、caspase-8、caspase-9 mRNA和蛋白表达。TUNEL染色检测大鼠脑组织细胞凋亡情况。结果 大鼠I/R可降低脑组织中Bcl-2表达,升高miR-503-5p、Bax、caspase-3、caspase-8、caspase-9表达及脑组织凋亡率。清热化瘀方治疗后可升高脑组织中Bcl-2表达,降低miR-503-5p、Bax、caspase-3、caspase-8、caspase-9表达及脑组织凋亡率。结论 清热化瘀方可抑制脑I/R大鼠脑组织细胞凋亡,其机制可能与抑制miR-503-5p、Bax及caspase家族表达、上调Bcl-2表达有关。  相似文献   

4.
王全胜  艾热提  路菲菲 《医学研究杂志》2019,48(7):146-149, 153
目的 评价炎性小体(NLRP3)在右美托咪定减轻大鼠肾缺血再灌注损伤中的作用。方法 清洁级成年雄性Sprague-Dawley 大鼠32只,采用数字表法随机分为假手术组(Sham组,n=8)、肾缺血再灌注损伤组(I/R组,n=8)、肾缺血再灌注损伤+右美托咪定预处理组(I/R+Dex预处理组,n=8)(于缺血前1h腹腔注射右美托咪定10μg/kg)和肾缺血再灌注损伤+右美托咪定后处理组(I/R+Dex后处理组,n=8),于再灌注后1h腹腔注射右美托咪定10μg/kg。肾缺血再灌注损伤模型采用夹闭双侧肾动脉45min后,恢复灌注24h。采用全自动生化分析仪测定各组血肌酐(Cr)和血尿素氮(BUN);酶联免疫吸附法(ELISA)检测血清TNF-α和IL-10的水平;TUNEL法检测各组凋亡细胞的数量;Western blot法检测NLRP3、凋亡信号(caspase-1、Cleaved caspase-1、Bcl-2)的表达。结果 与Sham组比较,I/R组血清Cr和BUN、炎性标志物(TNF-α和IL-10)水平及肾组织NLRP3、caspase-1和Bcl-2表达水平明显升高(P<0.05),同时肾小管凋亡细胞显著增多(P<0.05);与I/R组比较,I/R+Dex预处理组和I/R+Dex后处理组血Cr和BUN、TNF-α和IL-10水平均明显降低(P<0.05),肾组织NLRP3、caspase-1、Cleaved caspase-1和Bcl-2表达水平显著降低;肾小管凋亡细胞显著减少(P<0.05)。结论 右美托咪定可显著减轻大鼠肾缺血再灌注损伤,其可能与抑制NLRP3,降低炎性反应和细胞凋亡有关。  相似文献   

5.
目的探讨阿托伐他汀对兔心肌缺血再灌注诱导的细胞凋亡及Bcl-2、Bax蛋白表达的影响。方法30只雄性大白兔,随机分为假手术组(sham组)、缺血再灌注组(I/R组)及缺血再灌注加阿托伐他汀组(I/R AT组)。采用结扎、开放兔冠状动脉的方法,制作兔心肌缺血再灌注模型(缺血40min,再灌注2h),采用原位末端标记法(TUNEL法)检测心肌细胞凋亡情况,采用免疫组织化学法检测心肌中Bcl-2、Bax蛋白的表达情况。结果①I/R组凋亡指数明显大于sham组(P<0.01),而I/R AT组凋亡指数较I/R组显著减少(P<0.01)。②I/R组及I/R AT组Bcl-2蛋白表达百分率显著高于sham组(均P<0.01),且I/R AT组的Bcl-2蛋白表达也较I/R组明显上调(P<0.01);I/R组及I/R AT组的Bax蛋白表达明显强于sham组(均P<0.01),但I/R AT组的Bax蛋白表达弱于I/R组(P<0.01)。结论阿托伐他汀可以抑制心肌缺血再灌注诱导的细胞凋亡,减少心肌细胞凋亡数目,其机制可能与其上调心肌缺血再灌注时Bcl-2蛋白的表达、抑制Bax蛋白的表达有关。  相似文献   

6.
目的本研究探讨盐酸右旋美托咪啶(Dex)是否通过提高HIF-1α的表达减轻糖尿病大鼠肾缺血再灌注后损伤。方法高 脂高糖饮食8周后的SD大鼠,30 mg/kg链脲佐菌素(STZ)腹腔注射,建立糖尿病大鼠模型。以大鼠双肾动脉结扎60 min再灌注 120 min 的方法制作肾缺血再灌注损伤模型。分为普通大鼠组(Con),假手术组(sham),普通大鼠肾缺血再灌注损伤模型组 (Con+I/R [ischemia-reperfusion, I/R]),普通大鼠肾缺血再灌注损伤模型Dex治疗组(Con+I/R+Dex),糖尿病大鼠组(DM),糖尿 病大鼠Dex治疗组(DM+Dex),糖尿病大鼠地高辛(Dig)灌胃组(DM+Dig),糖尿病大鼠肾缺血再灌注损伤模型组(DM+I/R),糖 尿病大鼠肾缺血再灌注损伤模型Dex治疗组(DM+I/R+Dex),糖尿病大鼠地高辛灌胃后肾缺血再灌注损伤模型Dex治疗组 (DM+Dig+I/R+Dex)。夹闭双肾动脉前2 h给予腹腔注射Dex,其它组别腹腔注射等量生理盐水。地高辛作为HIF-1α的抑制 剂,对于地高辛组予地高辛片0.05 mg/(kg· d)灌胃1周。所有组别大鼠肾缺血再灌注后取血样,分离血浆,多功能生化仪检测肌 酐(Scr)和尿素氮(BUN)含量,Western blot 测定HIF-1α、cleaved caspase-3、Bcl-2、Bax,ELISA 检测HIF-1α、p-eNOS、eNOS, TUNEL法测定肾小球细胞凋亡比例。结果普通大鼠和糖尿病大鼠在肾缺血再灌注后Scr、BUN、HIF-1α、p-eNOS、eNOS表达 水平及细胞凋亡比例均显著升高(P<0.05);肾缺血再灌注损伤模型Dex治疗后Scr、BUN、p-eNOS、eNOS表达水平明显降低, HIF-1α表达水平明显升高,差异有统计学意义(P< 0.05);与Con+I/R相比,DM+I/R组Scr、BUN、p-eNOS、eNOS进一步升高;与 DM+I/R组相比,DM+I/R+Dex组Scr、BUN、p-eNOS、eNOS、cleaved caspase-3、Bax表达水平及凋亡细胞比例明显降低,HIF-1α 及Bcl-2表达明显升高;与DM+I/R+Dex组相比,DM+Dig+I/R+Dex组cleaved caspase-3、Bax表达水平及凋亡细胞比例明显升 高,HIF-1α及Bcl-2表达水平显著下降。结论Dex可能通过提高2型糖尿病大鼠HIF-1α的表达,抑制肾脏细胞的凋亡,减轻肾脏 细胞缺血再灌注后损伤。  相似文献   

7.
目的 探讨二甲双胍预处理对大鼠肾缺血再灌注损伤模型的影响及可能的机制。方法 健康清洁级成年雄性SD大鼠体重220~250g,共30只,采用数字表法将其随机分为3组(n=10):假手术组(Sham组),缺血再灌注组(I/R组)和二甲双胍组(metformin+I/R组)。metformin+I/R组在建立I/R模型前给予二甲双胍0.125mg/(kg·d)腹腔注射,共14天,Sham组和I/R组给予等容量生理盐水连续14天。给药结束后I/R组和metformin+I/R组采用切除右肾、夹闭左肾动静脉45min后恢复灌注的方法制备大鼠肾缺血再灌注模型,Sham组切除右肾、不夹闭左肾动静脉。分别于再灌注24h时抽取下腔静脉血测定血清肌酐(Cr)和尿素氮(BUN)浓度;然后处死大鼠取肾,光镜下观察肾组织形态学变化,免疫组化法测定Bcl-2、Bax、caspase-3表达,并行Tunel染色评估肾小管上皮细胞凋亡程度。结果 跟Sham组比较,I/R和metformin+I/R组的BUN、Cr均明显升高;同时,相比I/R组,metformin+I/R组的BUN和Cr均明显降低,且差异均有统计学意义(P<0.05)。免疫组化检测结果显示,跟Sham组比较,I/R组和metformin+I/R组Bcl-2、Bax、caspase-3表达明显增高;跟I/R组比较,metformin+I/R组Bax、caspase-3表达明显降低,同时Bcl-2的表达明显强于I/R组。Tunel结果显示,相比Sham组,I/R和metformin+I/R组肾小管上皮细胞凋亡数目明显增多;同时,metformin+I/R组肾小管上皮细胞凋亡数目明显少于I/R组,且差异有统计学意义(P<0.05)。结论 二甲双胍预处理能改善大鼠肾缺血再灌注损伤过程中肾功能损害,其机制可能与抑制肾小管上皮细胞凋亡有关。  相似文献   

8.
目的:研究PEP-1-CAT融合蛋白预处理对在体大鼠心肌缺血/再灌注损伤的保护作用,探讨其可能的作用机制。方法:60只SD大鼠随机分为5组:假手术组(Sham组)、缺血/再灌注组(I/R组)、低剂量预处理组(I/R+100μgPEP-1-CAT)、中剂量预处理组(I/R+300μgPEP-1-CAT)、高剂量预处理组(I/R+500μgPEP-1-CAT)。假手术组和缺血/再灌注组预先注射1mL的0.9%氯化钠溶液,低中高剂量预处理组分别注射100、300、500μg的PEP-1-CAT融合蛋白,7h后结扎冠状动脉前降支1h,再灌注2h后,检测心肌组织中的丙二醛(MDA)和血液中乳酸脱氢酶(LDH)及肌酸激酶(CK)含量,TTC染色测定心肌梗死面积,一步法TUNEL测定凋亡率,免疫荧光测定凋亡蛋白Bax和Bcl-2的表达。结果:与I/R组相比,低中高剂量预处理组显著减少心肌梗死面积(P<0.05或P<0.01),减少MDA的形成(P<0.01)和LDH、CK的释放,使凋亡率下降(P<0.01),下调Bax的表达,上调Bcl-2的表达,使Bax/Bcl-2比值下降。结论:PEP-1-CAT融合蛋白预处理能够明显减少心肌缺血/再灌注损伤,其机制可能与PEP-1-CAT融合蛋白具有抗氧化、抗凋亡作用有关。  相似文献   

9.
目的观察乙醛脱氢酶2(ALDH2)在糖尿病大鼠心肌缺血/再灌注凋亡发生中的作用。方法大鼠分为正常组、糖尿病组和ALDH2激动剂乙醇+糖尿病组。4周后行离体心肌缺血/再灌注(I/R)。测定复灌期间冠脉流出液中乳酸脱氢酶(LDH)含量。检测心肌组织细胞ALDH2、caspase-3的活性;RT-PCR测定左心室前壁心尖组织Bcl-2、Bax mRNA的表达。结果与正常大鼠I/R相比,糖尿病大鼠复灌期冠脉流出液中LDH释放增加,心肌组织caspase-3活性增加,ALDH2活性降低,Bcl-2/Bax mRNA比值降低;与糖尿病大鼠心肌I/R相比,ALDH2激动剂乙醇使得心肌复灌期间冠脉流出液中LDH释放减少,心肌caspase-3活性降低,ALDH2活性增高,Bcl-2/Bax mRNA比值增高。结论增强ALDH2在糖尿病大鼠心肌中的表达对缺血/再灌注损伤有明显的保护作用;其机制可能与抑制细胞凋亡的发生有关。  相似文献   

10.
目的 探讨不同预处理的心肌缺血/再灌注(I/R)动物模型中PPAR-γ表达对再灌注心律失常的影响.方法 32只SD大鼠分为4组(n=8):罗格列酮+I/R组(ROS组),GW9662+I/R组(GW组),I/R组(I/R组),假手术组(Sham组).采用冠状动脉左前降支结扎法制造缺血/再灌注动物模型,缺血30 min,再灌注2h.动态肢体Ⅱ导联心电图检测,荧光定量PCR检测PPAR-γ mRNA和Western blot检测PPAR-γ蛋白质的表达变化.结果 分别在术前、缺血30 min、再灌注1h、再灌注2h4个时间点检测心电图QRS波宽度增加幅度由大到小依次是ROS组、I/R组、GW组、Sham组;ROS组较其他组大鼠再灌注心律失常评分明显较高(P<0.05),GW组则相对减少.荧光定量PCR检测ROS组PPAR-γ mRNA表达水平明显上调(P<0.05),GW较I/R组和ROS组表达下调(P<0.05).PPAR-γ蛋白质表达结果与PPAR-γ mRNA相似.结论 心肌PPAR-γ表达上调可能增加心肌I/R动物模型再灌注心律失常的发生.  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…  相似文献   

20.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

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