低频超声联合微泡剂对肝癌细胞SMMC-7721的生物学效应

目的:研究低频超声联合微泡剂对肝癌细胞SMMC-7721的作用,为超声治疗的临床应用提供实验依据。方法:将细胞分为空白对照(A)组、单纯微泡剂(B)组、单纯超声(C)组和超声联合微泡剂(D)组。再按超声辐照60、90、120和150 s,C组分为C1、C2、C3、C4,D组分为D1、D2、D3、D4。MTT法观察细胞增殖的抑制作用;分光光度法检测细胞培养液中活性氧(ROS)和超氧化物歧化酶(SOD)活力的变化,流式细胞仪(FCM)检测细胞凋亡。结果:超声联合微泡剂能够显著抑制细胞增殖;C3、D3组培养液中ROS活力显著高于A、B两组(P<0.01),SOD活力明显低于A、B两组(P<0.01),且D3组效应比C3组更为明显;C3、D3组细胞凋亡率明显高于A、B组(P<0.01),D3组高于C3组(P<0.05)。结论:低频超声联合微泡剂可能是通过增加细胞外ROS的活力来诱导对细胞的损伤和凋亡。     

核素131I联合低频超声微泡治疗兔VX2肝肿瘤的实验研究

目的:探讨核素131I联合低频超声微泡治疗接种于新西兰大白兔肝左叶VX2肝肿瘤的疗效。方法:将成功建立肿瘤模型的32只荷瘤兔随机分为4组(8只.组-1):A组,不作任何处理;B组,使用单纯核素131I治疗;C组,使用单纯低频超声微泡治疗;D组,应用核素131I联合低频超声微泡连续治疗。在2周治疗期间连续进行B超检查,观察各组肿瘤生长及体积变化情况。核素治疗后第1、2、4、8、14天分别对B、D两组行单光子发射型电子计算机断层显像(SPECT),并计算不同时间肿瘤区放射性计数值。治疗2周后取部分肿瘤组织行病理学观察。结果:B、C、D 3组肿瘤体积及增长率均小于A组(P<0.05);B组放射性计数的衰减率明显高于D组(P<0.05);病理学检查发现B、C、D组坏死较A组严重,其中D组最明显。结论:核素131I联合低频超声微泡治疗时能够产生协同效应,明显提高疗效。     

超声辐射微泡剂对家兔卵巢血管的影响

目的探讨低功率超声辐射微泡剂对家兔卵巢微血管的影响,为该方法在临床应用提供动物实验依据。方法30只生育期的家兔随机分为2个实验组(A、B组)和1个对照组(C组),每组10只。对A、B组家兔一侧耳缘静脉注射微泡剂,然后以低功率低频率(20 kHz,0.5 W)超声照射家兔腹部的4个象限各120 s,照射结束后在24 h(A组)、7 d(B组)解剖实验组家兔,同时解剖对照组(未行微泡剂注射及照射)家兔,取双侧卵巢,观察每组卵巢血管内血栓形成及微血管密度改变。结果(1)超声干预后A、B两组家兔双侧卵巢血管内血栓形成率分别为(33.67±2.83)%和(2.96±0.80)%,对照组为0,A组与B、C两组比较有统计学差异(P<0.05);B、C两组比较无统计学差异(P>0.05)。(2)超声干预后,A、B组微血管密度分别为11.00±2.83和24.00±4.80,对照组为27.00±3.09;A组与B、C两组比较有统计学差异(P<0.05);B、C两组比较无统计学差异(P>0.05)。结论低功率超声辐射微泡剂对家兔卵巢正常微血管的影响是可逆的。     

自制辅助装置完成超声微泡介导pEGFPC1-Akt1基因转染

目的初步探讨超声微泡介导基因转染的可行性.方法 自制辅助装置,联合应用超声辐射和SonoVue超声微泡介导pEGFPC1-Akt1基因转染293FT细胞,用增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)作为报告基因,转染后荧光显微镜下计数细胞转染率.实验分4组:A组,单纯质粒组;B组,质粒+微泡组;C组,质粒+超声组;D组,质粒+超声+微泡组.结果自制辅助装置制作成功,符合实验要求的条件.转染结果为 A组和B组未见荧光表达,C组转染率约为4.26%,D组转染率为34.56%,D组转染率高于C组(P<0.05).结论 使用自制辅助装置,联合应用超声辐射和微泡法能够有效介导pEGFPC1-Akt1基因转染.     

联合应用angiostatinK(1-3)和endostatin裸DNA对人脑胶质瘤生长的抑制作用

目的 :探讨联合应用angiostatinK(1 3) {ASK(1 3) }和endostatin(ES)裸DNA对人脑胶质瘤生长的影响和作用特点 .方法 :在 1 5只裸鼠皮下接种SHG4 4人脑胶质瘤细胞并分A ,B和C组 (n =5 ) ;在接种后第 1 2日 ,A组 :给予瘤体内注射 2 0 μL脂质体包裹的各 5 μg的 pcDNA S ASK (1 3)和pcDNA S ES质粒 ;B组 :瘤体内注射 2 0 μL脂质体包裹的 1 0μgpcDNA 3.1质粒 ;C组 :瘤体内注射 2 0 μL无菌生理盐水 ;3d后重复注射 1次 .每日定时观察肿瘤生长情况 .4 0d后处死裸鼠 ,检测和计算肿瘤体积、坏死率、血管数和抑瘤率 .结果 :A ,B和C组肿瘤的终体积分别是 4 .4 1 6± 1 .88cm3 ,8.0 34± 1 .85cm3 和 8.0 5 6± 1 .91cm3 ,A组抑瘤率为 4 6 .8% ;A ,B和C组肿瘤的平均坏死率分别为 39.1 2± 7.35 % ,9.1 7± 7.89%和 9.2 1± 7.4 8% ,A与B或C组比较差异显著 (P <0 .0 1 ) ,B ,C组比较差异不显著 (P >0 .0 5 ) .3组的血管计数分别为 3.92± 1 .89,1 1 .8± 2 .0 4和 1 2 .0± 2 .2 1 ,A与B或C组比较差异显著 (P <0 .0 1 ) ,B ,C组比较差异不显著 (P >0 .0 5 ) .结论 :联合应用ASK(1 3)和ES裸DNA可抑制胶质瘤的血管生成、导致其坏死增加 ,进而抑制肿瘤生长 .其作用机制有待深入研究 ,该实验为胶质瘤的抗血管生成     

去蛋氨酸/缬氨酸空肠喂饲荷瘤大鼠对肿瘤生长的影响及其作用机制

目的 :探讨蛋氨酸 /缬氨酸缺乏对肿瘤生长的影响及其作用机制。方法 :建立SD大鼠Walker 2 5 6癌肉瘤空肠喂饲模型 ,分A、B、C、D 4组 ,分别以平衡氨基酸、去蛋氨酸、平衡氨基酸、去缬氨酸空肠喂饲 6d ,注入3H 蛋氨酸/缬氨酸 740kBq 0 .5 ,1,2 ,4h后分别测定肿瘤组织核酸及蛋白质水解产物的3H掺入率 ;检测移植瘤重、抑瘤率。结果 :A、B组瘤重 (g)分别为 (3.5 3± 0 .2 0 )及 (2 .30± 0 .2 7) (P <0 .0 1) ,C、D组瘤重 (g)分别为 (3 .6 4± 0 .5 0 )及 (2 .6 3± 0 .37) (P <0 .0 1)。B、D组抑瘤率分别为 32 %及 2 8%。B、D组各时点蛋白质3H掺入率分别为 (0 .5 0 0± 0 .0 2 0 ) %～(3.6 70± 0 .110 ) %及 (1.887± 0 .0 2 0 ) %～ (3 .813± 0 .0 76 ) % ,分别低于A、C组 ;B组RNA、DNA的3H掺入率分别为(0 .2 73± 0 .0 75 ) %及 (0 .2 31± 0 .0 5 2 ) % ,低于A组。结论 :去蛋氨酸 /缬氨酸空肠喂饲荷瘤大鼠可抑制肿瘤蛋白质的合成 ,缺乏蛋氨酸还可影响核酸代谢 ,从而抑制肿瘤生长     

不平衡氨基酸对肿瘤细胞生长和细胞凋亡的影响

目的比较几种不平衡氨基酸营养支持对肿瘤生长和细胞凋亡的影响。方法荷W alker-256癌肉瘤SD大鼠,空肠喂饲肠外营养制剂10 d,据所喂饲制剂中氨基酸组成的不同分为:A组(平衡氨基酸组)、B组(增量精氨酸不平衡氨基酸组)、C组(去蛋氨酸不平衡氨基酸组)、D组(去缬氨酸不平衡氨基酸组)、E组(增量精氨酸、去蛋氨酸、去缬氨酸不平衡氨基酸组)。肿瘤体积增长速度、肿瘤质量、肿瘤质量/尸重,肿瘤组织PCNA指数,肿瘤细胞周期各时相百分比以及DNA倍性作为观察肿瘤生长速度的指标,并观察肿瘤细胞凋亡状况。结果上述各肿瘤生长指标值显示:B、C、D组与A组相比,除DNA倍性外,其余各指标值均降低(P<0.01),但B、C、D组间比较差异无显著性(P>0.05)。E组与B、C、D组相比较,所有指标值均显著降低(P<0.05);A、B、C、D、E组细胞凋亡指数分别为:(6.5±1.4)%、(13.7±2.8)%、(12.9±3.1)%、(14.1±4.4)%、(28.9±6.5)%,E组较A、B、C、D组凋亡指数明显升高,B、C、D组较A组亦升高明显(P<0.01),、B、C、D组间差异无显著性。结论平衡氨基酸促进肿瘤生长;增量精氨酸、去蛋氨酸、去缬氨酸3种不平衡氨基酸对肿瘤生长有同等抑制作用并诱导肿瘤细胞凋亡;增量精氨酸、去蛋氨酸、去缬氨酸复合不平衡氨基酸对肿瘤生长抑制及诱导肿瘤细胞凋亡作用更显著。     

马钱子碱经皮给药对乳腺癌种植瘤中VEGF和COX-2蛋白表达的影响

目的:探讨马钱子碱经皮给药对乳腺癌种植瘤的抑制机制,为乳腺癌的治疗提供新的途径。方法 :培养人乳腺癌细胞株MDA-MB-231,取对数生长期细胞制成单细胞悬液。20只雌性Balb/c-nu/nu裸鼠麻醉后,无菌条件下,局部肌肉注射乳腺癌单细胞悬液0.2 mL,复制乳腺癌肿瘤模型。20只荷瘤裸鼠随机分为4组:马钱子碱高剂量组(A组)、中剂量组(B组)、低剂量组(C组)和模型组(D组),分别给予20%、10%、5%浓度的马钱子碱膏(经腹腔给药,每日总剂量不超过LD50)和凡士林经皮给药,涂抹裸鼠的整个腹部和造模的部位,每次1 g,每日4次,连续15 d。观察各组裸鼠生存状态和肿瘤生长情况,评价抑瘤率;检测肿瘤组织中血管内皮生长因子(VEGF)和环氧合酶-2(COX-2)蛋白表达。结果:①肿瘤大小和抑瘤率:A组和B组裸鼠生存状态良好,肿瘤引起的活动障碍较轻,肿瘤生长均受到不同程度的抑制,肿瘤生长较C组和D组迟缓,各组的抑瘤率分别为A组53%、B组45%、C组27%(P<0.05);而C组和D组裸鼠出现活动障碍、瘫痪、消瘦和精神萎靡,肿瘤体积较大。②免疫组化结果:VEGF和COX-2马钱子碱A组分别为0.22±0.01和0.15±0.02,B组分别为0.25±0.01和0.18±0.01,C组分别为0.26±0.02和0.20±0.01,D组分别为0.42±0.02和0.35±0.03。VEGF和COX-2蛋白在肿瘤组织中表达的程度与模型组比较是随马钱子碱剂量的增加而逐渐减弱,差异均有统计学意义(P<0.05)。结论:马钱子碱经皮给药能抑制乳腺癌种植瘤中VEGF和COX-2的蛋白表达。     

低机械指数超声介导声诺维对体内外细胞活性影响的研究

目的: 探讨低机械指数超声介导声诺维对人脐静脉内皮细胞和脑缺血模型大鼠脑细胞活性影响的
研究。方法: 体外实验: 分为空白对照组( a 组) ; 单纯微泡剂组( b 组) ; 超声微泡组( c 组) ,应用MTT 测线粒体活
性。体内实验: A 组为单纯超声辐照组,B 组为高剂量造影剂加超声辐照组,C、D、E 组分别为低、中、高剂量造影
剂加脑缺血造模超声辐照组。TUNEL 法检测脑细胞凋亡情况。结果: 体外实验显示,超声联合微泡剂( c 组) 对
内皮细胞活性的影响有显著意义( P＜0．001) ,但c 组间差异无统计学意义。体内实验显示,凋亡细胞计数,C、D、
E 组与A、B 组间有显著统计学差异( P＜0．01) ,但在缺血C、D、E 组间以及A、B 组间无统计学差异( P＞0．05) 。结
论: 低机械指数超声造影可增强细胞通透性,但在控制好各种因素的前提下可安全应用于临床超声。     

核素^131I联合低频超声微泡治疗兔VX2肝肿瘤的实验研究

目的：探讨核素131I联合低频超声微泡治疗接种于新西兰大白兔肝左叶VX2肝肿瘤的疗效。方法：将成功建立肿瘤模型的32只荷瘤兔随机分为4组（8只.组-1）：A组,不作任何处理;B组,使用单纯核素131I治疗;C组,使用单纯低频超声微泡治疗;D组,应用核素131I联合低频超声微泡连续治疗。在2周治疗期间连续进行B超检查,观察各组肿瘤生长及体积变化情况。核素治疗后第1、2、4、8、14天分别对B、D两组行单光子发射型电子计算机断层显像（SPECT）,并计算不同时间肿瘤区放射性计数值。治疗2周后取部分肿瘤组织行病理学观察。结果：B、C、D 3组肿瘤体积及增长率均小于A组（P〈0.05）;B组放射性计数的衰减率明显高于D组（P〈0.05）;病理学检查发现B、C、D组坏死较A组严重,其中D组最明显。结论：核素131I联合低频超声微泡治疗时能够产生协同效应,明显提高疗效。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.