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1.
离子对色谱法测定昔奈福林含量   总被引:2,自引:0,他引:2  
采用离子对高效液相色谱法测定陈皮及胃苏冲剂中昔奈福林的含量。色谱条件为:ResolveC18色谱柱,柱温40℃;流动相为26.2mmol/L十二烷基磺酸钠水溶液-乙腈-磷酸(700∶300∶0.5);流速0.8ml/min;检测波长276nm;线性范围0.01~0.1mg/ml,r=0.9998,加样回收率为97.2%,RSD为2.1%。  相似文献   

2.
建立了用反相离子对高效液相色谱法测定人血浆中阿昔洛韦浓度的方法。色谱柱采用AlltechC_18分析柱,以0.04mol/L氯化钠-甲醇-IPR-B7离子对色谱试剂混合液(100:15:0.6;v/v)为流动相。检测波长为254nm,肾上腺素为内标。血浆样品经高氯酸沉淀蛋白后直接进样测定。阿昔洛韦浓度在40~1600ng/ml范围内线性良好,r=0.9992.测定含阿苷洛韦160ng/ml的血浆样品,其日内(n=7)及日间的RSD分别为4.7%和3.0%。平均回收率为98.2±5.3%。测定了10名健康志愿者单次口服阿昔洛韦片剂400mg后不同时间的血药浓度并计算了有关的药代动力学参数。  相似文献   

3.
本文用紫外分光光度法测定了盐酸氯胺酮注射剂的含量,溶剂为0.1mol/L盐酸液,测定波长269nm,盐酸氯胺酮在0.1~0.35mg/ml浓度范围内线性关系良好,平均回收率为100.0%,相对标准差为0.3%。  相似文献   

4.
用苯酚-硫酸法进行了油松果多糖的含量测定,并考察了苯酚与硫酸的用量及反应温度等因素对显色反应的影响,最大吸收波长490nm,测得多糖在10~70μg/ml的浓度范围内与吸收度呈线性关系(r=0.9999 n=6),平均回收率为99.9%,变异系数为1.2%,最低检测浓度为0.5μg/ml。  相似文献   

5.
建立了离子对高效液相色谱法测定米雅利桑爱儿A颗粒剂中泛酸钙、维生素B6及维生素B2的含量。采用C18柱,以含019%(体积分数)磷酸的001mol/L庚烷磺酸钠溶液甲醇(74∶26)为流动相,检测波长为225nm。线性范围分别为:泛酸钙0064~032mg/ml,维生素B60064~032mg/ml,维生素B20016~008mg/ml;回收率分别为995%,982%,1002%;精密度(RSD)分别为08%、06%、08%。  相似文献   

6.
高效液相色谱法测定奥美拉唑血浆药物浓度   总被引:1,自引:0,他引:1  
用反相高效液相色谱法测定人体血浆中奥美拉唑的浓度,采用国产填料(YWG-C18,10μm)反相色谱柱,1.0%三乙胺甲醇:水(60:40,V:V)为流动相,心律平为内标,紫外检测液长为302nm,方法回收率分别为98.03%(50mg/ml)101.55%(400mg/ml)和103.13%(1000mg/ml),相应变异系数(CV)分别为2.96%(n=3),1.92%(n=3)和1.04%(n  相似文献   

7.
采用反相高效液相色谱法同时测定了洗必泰及有关杂质对氯苯胺浓度。用Zorbax-C_8柱,0.2mol/LNaH_2PO_4(pH3.0)-甲醇(49.5:50.5)为流动相,非那西丁为内标,240nm波长检测。本法简便准确,重现性好。洗必泰和对氯苯胺分别在15~200μg/ml和60~1200μg/ml浓度范围内线性关系良好(r=0.9999),最低检测限分别为1.33μg/ml和7.5μg/ml(S/N=3:1),平均回收率分别为100.1%和103.1%。相对标准偏差R50%分别为1.41%和1.14%。  相似文献   

8.
目的:研究快速测定奶制品中微量奶,方法:吐温-80苯芴酮分光光度法,结果:线性范围0μg/ml~10μg/ml,相关系数0.9994,测定25个牛奶制品,检出20份,范围0.087mg/L~1.95mg/L,样品加标回收率91.3%~97.6%,变异系数1.7%~3.4%,结论:该法灵敏,准确,快速,适宜大批样品测定。  相似文献   

9.
用硝酸钙添加剂形成难熔的氟化钙,高温灰化进行样品前处理。以柠檬酸钠—氯化物—冰醋酸所组成的总离子强度调节剂(TISAB)的pH值控制在5.0~5.5之间,用氟离子选择电极测定生物样品中氟含量,间接求出5-FU、FT-207。可测血浆氟线性范围0.005~0.05和0.05~20μg/ml。相关系数为0.9998和0.9991,检测下限0.005μg/ml.血浆5-FU可测范围0.00~6840.39μg/ml。测定血浆中氟及5-FU标准差为0.005、0.08;变异系数为2.3%、4.5%;回收率为97.5%、84.0~111.2%。对大剂量口服FT-207的狗,测定内脏FT-207,获满意结果。本法也适用于其它含氟药物的测定  相似文献   

10.
30例健康育龄男性精液的体外杀精试验显示:盐酸异丙嗪具有快速强力杀精作用,当药物浓度在186mmo·L-1(06mg/ml)以上时,在10s内死亡;在0075~124mmol·L-1(0.25~0.4mg/ml)浓度范围内,其杀精作用随药物浓度增加和作用时间延长而增强。当浓度为031mmol·L-1(0.1mg/ml)作用时间为30min时,杀精作用可接近或达到100%;而浓度为12mmol.L-1(0.4mg/ml)时,5min内即可杀灭全部精子  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

20.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

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