首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 62 毫秒
1.
目的:研究力竭运动后心肌变色酸-2R亮绿染色的表现。方法:50只健康成年雄性SD大鼠随机分为一次力竭运动组和对照组,力竭组进行一次力竭游泳运动,运动后分别于即刻、6h、12h、24h取心室肌组织进行石蜡切片及变色酸2R-亮绿染色。结果:一次力竭运动后各时相大鼠心肌变色酸2R一亮绿染色显示心肌细胞浆存在点、片状红色缺血缺氧改变,以运动后6h最显著。结论:变色酸--2R亮绿染色可以敏感地反应运动性心肌微损伤的病理改变,可以作为建立运动性心肌微损伤实验动物模型的评价指标。  相似文献   

2.
目的:探讨力竭运动后大鼠心肌肌钙蛋白-T(cTnT)分布及含量的变化。方法:将50只健康成年雄性SD大鼠随机分为一次力竭运动组和安静对照组,运动组采用游泳运动方式分别于力竭运动后即刻6、h、12 h、24 h取材,应用免疫荧光技术和图像分析方法对大鼠心肌肌钙蛋白-T分布及含量的改变进行研究。结果:力竭运动后各时相大鼠心肌cTnT分布变得紊乱,在心肌细胞间及细胞内的分布不均一;一次力竭后即刻、6 h和12 h右心室cTnT含量高于对照组(P〈0.01)。结论:一次力竭运动均可使心肌cTnT分布变得紊乱,这可能是导致心肌机械功能异常的原因之一。  相似文献   

3.
目的 探讨麝香保心丸对一次性力竭运动大鼠心肌损伤标记物和C反应蛋白的影响。方法 选择雄性Wistar大鼠52只,随机分成实验组和对照组,每组26只。两组大鼠每天自由进食饮水相同,实验组大鼠每天麝香保心丸2粒(每粒22.5mg)分2次灌胃,共两周。两组均接受力竭游泳运动,制成力竭运动动物模型,分别测定和比较两组大鼠血清和心肌组织肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、肌钙蛋白T(TnT)、B型脑钠肽(BNP)和C反应蛋白(CRP)水平。结果 大鼠平均力竭运动游泳时间实验组较对照组明显延长(P<0.01)。实验组血清和心肌组织CK、CK-MB、TnT、BNP和CRP水平均明显低于对照组(P<0.05)。结论 麝香保心丸可显著降低力竭运动大鼠血清和心肌中CK、CK-MB、TnT、BNP和CRP水平,能够减轻力竭运动后心肌损伤。  相似文献   

4.
目的:探讨力竭性离心运动对腓肠肌波形蛋白和结蛋白的时序性影响.方法:50只8周龄SD大鼠随机分为安静组(C)和运动组(0 h,3h,24 h,48 h);运动组进行重复三天的力竭性离心运动,免疫组化检测大鼠腓肠肌不同恢复时相波形蛋白和结蛋白的表达.结果:离心运动后腓肠肌Vimentin的阳性表达面积明显增大,而且在运动...  相似文献   

5.
力竭性运动对小鼠心,肾组织MDA与SOD活性的影响   总被引:4,自引:0,他引:4  
雄性昆明种小鼠22只,按体重随机分为运动组与安静组。运动组经2周游泳适应性训练,在一次力竭性负重游泳运动后对小鼠的心、肾组织中丙二醛、超氧化物歧化酶进行测定;安静组作为对照。结果显示:力竭性运动可命可使小鼠心、肾组织中MDA含量增加,SOD总活性增强。提示力竭性运动对小鼠心、肾组织均有自由基损伤作用,而SOD活性的升高,可能仅仅是一种代偿性适应现象。  相似文献   

6.
目的 探讨不同运动量游泳运动对大鼠左心室心肌细胞超微结构的影响,以期为运动对心肌的生理和病理变化研究提供参考资料.方法 SD大鼠分为不同运动量运动组和力竭运动组,前者进行12周的低、中和高运动量游泳运动,后者进行1次力竭游泳运动.取左心室心肌组织进行常规透射电镜样品制备,透射电镜观察左心室心肌细胞超微结构的变化.结果 低运动量运动对大鼠左心室心肌细胞结构影响不大;中运动量运动提高心肌肌节长度,线粒体数量和体积增加,嵴变得较密集;高运动量运动对心肌细胞产生轻微破坏,肌节变短,核膜内陷,核质凝缩,线粒体嵴疏松模糊.力竭运动后即刻,对心肌细胞的细胞核和线粒体影响较大,肌节长度缩短.力竭后24 h,心肌细胞出现细胞核和线粒体损伤减轻,肌节长度增加的变化,但肌纤维排列严重紊乱.结论 高运动量和力竭运动可以导致左心室心肌细胞超微结构破坏.  相似文献   

7.
雄性昆明种小鼠22只,按体重随机分为运动组与安静组。运动组经2周游泳适应性训练,在一次力竭性负重游泳运动后对小鼠的心、肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)进行测定;安静组作为对照。结果显示:力竭性运动可使小鼠心、肾组织中MDA含量增加,SOD总活性增强。提示力竭性运动对小鼠心、肾组织均有自由基损伤作用,而SOD活性的升高,可能仅仅是一种代偿性适应现象。  相似文献   

8.
目的 观察1周力竭游泳运动后大鼠下丘脑室旁核(PVN)NF-κB基因的表达变化及变化规律. 方法 将35只大鼠随机分为对照组(n=5)和力竭组(n=30),力竭组大鼠进行1周力竭游泳运动,最后一次力竭运动结束后分即刻(0h),6 h,10 h,14 h,20 h,24 h共6个时间点,采用组织化学SABC法观察大鼠下丘脑室旁核内NF-κB核转录因子的定位和分布表达规律,采用Western Blot法研究下丘脑室旁核NF-κB含量变化,通过图像分析系统和统计学软件进行图像和数据分析.  相似文献   

9.
目的以大鼠1周力竭游泳为模型,观察力竭运动后大鼠杏仁核NF—KB在不同时间点的表达变化。方法把40只大鼠随机分为对照组和力竭组。力竭组进行1周力竭游泳训练后,用免疫组织化学技术SABC法检测即刻,6h,8h,10h,12h,14h,24h等不同时间点大鼠脑杏仁核NF—KB的表达变化,通过图像分析系统和统计学软件进行图像和数据分析。结果与对照组相比,力竭组杏仁核NF—kB表达在6h开始升高(P〈0.05),8—10h达高峰(P〈0.05),12—14h开始明显回落(P〈0.05),24h恢复到正常水平(P〉0.05)。结论杏仁核NF—KB信号系统参与力竭游泳运动引起的氧化应激及神经系统功能活动的改变。  相似文献   

10.
目的 以大鼠1周力竭游泳为模型,观察力竭运动后大鼠杏仁核NF-κB在不同时间点的表达变化.方法 把40只大鼠随机分为对照组和力竭组.力竭组进行1周力竭游泳训练后,用免疫组织化学技术SABC法检测即刻,6 h,8 h,10 h,12 h,14 h,24 h等不同时间点大鼠脑杏仁核NF-κB的表达变化,通过图像分析系统和统计学软件进行图像和数据分析.结果 与对照组相比,力竭组杏仁核NF-κB表达在6 h开始升高(P<0.05),8-10 h达高峰(P<0.05),12-14 h开始明显回落(P<0.05),24 h恢复到正常水平(P>0.05).结论 杏仁核NF-κB信号系统参与力竭游泳运动引起的氧化应激及神经系统功能活动的改变.  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

20.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号