^3H—精氨酸转化测定一氧化氮合酶活性

建立＾３Ｈ－精氨酸转化测定原生型和诱生型一氧化氮合酶的方法。方法 以二乙氨乙基（ＤＥＡＥ）纤维素柱层析和２、５－ＡＤＰ琼脂糖亲合层析法纯化牛、猪及大鼠小脑一氧化氮合酶、用＾３Ｈ－精氨酸转化法测定一氧化氮合酶活性。结果 猪、大鼠小脑组织匀浆一氧化氮合酶活性分别为６６９、６８８ｐｍｏｌ．ｍｇ＾－１．ｍｉｎ＾－１分别纯化了４４６０倍、２６４６倍。获得率分别为２．７％、２７％。纯化后的猪和大鼠小脑一氧化氮     

四物汤刺激淋巴细胞^3H—TdR掺入及分泌IL—2

实验表明四物汤能显著促进自发性和ＣｏｎＡ刺激的小鼠淋巴细胞＾３Ｈ－ＴｄＲ掺入,显著促进正常小鼠,正常大鼠淋巴细胞和混合脾淋巴细胞产生白细胞介素－２（ＩＬ－２）,但未能显著提升血虚大鼠脾淋巴细胞和混合脾淋巴细胞分泌ＩＬ－２的水平。结果说明 汤能显著增加机体的细胞免疫功能。     

Tissue Distribution of [^3H]—Nicotine in Rats

This study was conducted in adult male Sprague-Dawley rats to determine thedistribution of [3~H]-nicotine in blood and tissues following a bolus injection and a constantinfusion of pure nicotine.The animals were anesthetized and injected with either 0.5 ml ofnicotine solution or given a constant infusion of the same nicotine solution with identicalamounts of radioactive nicotine.After sacrifice,blood,brain,trachea.salivery gland,esophagus,lung,heart,liver,fundus,antrum,spleen,pancreas,duodenum,jejunum,ileum,cecum,colon,kidneys,adrenal gland,and testes were collected and measured for radioactivityby scintillation counting.The distribution of nicotine was found highest in kidneys by bothroutes of administration.Higher accumulations were also found in salivary and adrenalglands,fundus,antrum,duodenum,jejunum,ileum and colon.Retention of nicotine viaconstant infusion was significantly higher in esophagus,fundus antrum,spleen,cecum,pancreas,testes,heart and muscle when compared with bolus injection.Six-fold     

^3H—SC1001的吸收,分布和排泄

SC1001 is a new Synthesized CNS depressant. In this paper, the absorption, distribution and excretion of 3H-SC1001 in mouse and rats were studied by radioactivity trace method. The experiment showed that SC1001 was absorbed rapidly after administering orally and intramuscularly. Maximum radioactivity was found at 0.13h and 0.33h. The rapidly and widely distribution was demonstrated after absorption, the level of radioactivity was the highest in the blood, then in liver, kidney, intestine lung, heart, spleen, and brain. 3H-SC1001 could easily pass through the blood-brain barrier. 3H-SC1001 was eliminated in faeces and then in urine. In 3 days the cumulative radioactivity excretion was 32.0% in urine and 74.04% in faeces, t1/2 of p.o. was 5.69h and t1/2 of i.m. was 6.91h.     

一种新的^3H—TdR掺入法抗癌药敏试验

作者对琼脂-液体双层抗癌药敏试验系统加以改进,建立了更为简便有效的试验系统。经对84例临床肺癌患者活标本测试,成功率达85%。将肺癌细胞对长春新碱(VCR),丝裂霉素(MMC),顺铂(DDP)和阿霉素(ADM)的敏感界限,以1/10血液峰值浓度下细胞存活率表示,并分别定为50%、40%、30%和30%时,测试结果与文献报告的肺癌临床单药化疗有效率近似。本法与干细胞法作平行试验测试10个肺癌细胞系对4种药物的敏感性,两法符合率为83%。测试4例肺癌活检标本,两法符合第为100%。作者认为本试验系统具有临床推广应用价值。