复方丹参注射液对CIA大鼠滑膜细胞IL-1β mRNA表达的影响

目的：研究复方丹参注射液对Ⅱ型胶原蛋白诱导性大鼠关节炎模型（CIA）滑膜细胞IL－1βmRNA表达的影响。方法：用Ⅱ型胶原蛋白诱导大鼠关节炎模型，以逆转录－多聚酶链反应（RT－PCR）半定量法测定模型大鼠滑膜细胞IL－1βmRNA的表达。结果：复方丹参大剂量可以明显下调CIA大鼠滑膜细胞IL－1βmRNA的表达水平。结论：复方丹参治疗类风湿性关节炎的机制可能与下调滑膜细胞IL－1βmRNA表达的水平有关。     

青蒿琥酯对胶原诱导性关节炎大鼠滑膜细胞增殖及TNF-α和IL-1β分泌的影响

摘要：目的：研究青蒿琥酯对胶原诱导性关节炎大鼠滑膜细胞增殖和细胞因子分泌的影响,探讨青蒿琥酯对类风湿关节炎治疗作用的可能机制。方法：制备胶原诱导性关节炎(Collagen Induced Arthritis, CIA)大鼠模型,取其滑膜组织进行原代培养,MTT法检测药物对滑膜细胞增殖的影响,ELISA检测药物对滑膜细胞分泌IL-1β和TNF-α等炎细胞因子的影响。结果：与对照组比较, 青蒿琥酯对滑膜细胞增殖有显著抑制作用(P<0.05);与对照组比较,青蒿琥酯各组滑膜细胞分泌IL-1β和TNF-α水平均明显下降(P<0.05)。结论：青蒿琥酯能显著抑制CIA大鼠滑膜细胞的增殖,并能下调滑膜细胞分泌TNF-α和IL-1β水平。     

桂枝芍药知母汤对Ⅱ型胶原诱导性关节炎大鼠IFN-γ及IL-4水平的影响

目的研究桂枝芍药知母汤对Ⅱ型胶原诱导性关节炎大鼠的治疗作用,并探讨其作用机制。方法采用牛Ⅱ型胶原诱导的关节炎模型(Collagen-induced arthritis,CIA),灌胃给药21 d后观察大鼠体重变化、足肿胀度;采用ELISA法测定大鼠滑膜和血清中的白细胞介素4(IL-4)水平,小鼠巨噬细胞NO2-释放法测定大鼠滑膜和血清中干扰素γ(IFN-γ)活性。结果桂枝芍药知母汤能改善CIA大鼠体重变化,降低原发性和继发性足肿胀度,下调CIA大鼠滑膜和血清IFN-γ、IL-4水平。结论CIA大鼠滑膜中IFN-γ、IL-4水平均升高,血清中以IFN-γ升高为主。桂枝芍药知母汤抗风湿作用,可能通过下调CIA大鼠滑膜和血清IFN-γ、IL-4水平,从而调节Th1/Th2平衡。     

热痹康汤对胶原诱导性关节炎大鼠血清IL-1β及IL-4的影响

目的：观察热痹康汤对胶原诱导性关节炎大鼠血清白介素-1β（IL-1β）及白介素-4（IL-4）的影响,以初步揭示热痹康汤治疗类风湿关节炎的机理,并为其在临床上治疗类风湿关节炎提供实验依据。方法：采用牛Ⅱ型胶原蛋白诱导胶原诱导性关节炎（CIA）大鼠模型,以甲氨蝶呤作对照,观察热痹康汤对各组大鼠的关节肿胀指数以及血清IL-1β、IL-4水平的影响。结果：热痹康汤能降低CIA大鼠血清IL-1β的水平,升高IL-4的水平,作用与甲氨蝶呤相当。结论：热痹康汤能下调CIA大鼠血清IL-1β水平、提高IL-4的水平,是其治疗类风湿关节炎的作用机理之一。     

复方丹参注射液治疗风湿性疾病的研究

目的观察复方丹参注射液对风湿性疾病的疗效及其机理。方法用复方丹参注射液20ml加入液体中静滴,1次/d,20天1疗程,对124例类风湿性关节炎(RA)、60例强直性脊柱炎(AS)和60例骨性关节炎(OA)患者进行治疗,从显效率和总有效率、症状体征的改善、激素使用率、不良反应发生率和问卷调查等方面观察临床疗效;用大鼠CIA模型,从关节病理、自由基、血粘度、细胞凋亡、细胞因子表达等角度研究疗效机理。结果临床研究发现以该药为主,配合中医辨证治疗,总疗效优于对照组,其中RA显效率、AS显效率和总有效率有显著性差异,对症状体征的缓解明显优于对照组,激素使用率和使用量以及不良反应发生率均明显低于对照组,问卷调查显示疼痛缓解和生存质量均优于对照组;实验研究发现能减轻关节局部炎症反应,升高血清SOD含量和SOD/MDA比值,降低全血粘度,诱导滑膜细胞凋亡,抑制IL-1β、TNF-α及PGE2等炎症介质的生成,下调IL-1βmRNA、IL-6mRNA表达水平,并可下调RA患者滑膜细胞的VEGFmRNA表达。结论复方丹参注射液治疗风湿性疾病有良好的疗效。     

电针对胶原性关节炎大鼠的镇痛效应和抗体、IL-1β水平的影响

目的：观察电针对胶原诱导性关节炎大鼠痛阈、β－内啡肽、抗Ⅱ型胶原抗体及白介素－1β水平（IL－1β）等的影响，以探索电针镇痛与免疫调节的双重作用及其相关性。方法：Wistar雄性大鼠经牛Ⅱ型胶原皮内注射接种诱导胶原性关节炎动物模型，在造模后第3天起在“足三里”穴进行电针刺激，连续19天。采用辐射热甩尾法测定大鼠痛阈，Wood氏法评估关节炎指数，酶联免疫吸附实验（ELISA）检测关节炎大鼠血清抗Ⅱ型胶原抗体水平和脾脏白介素－1β含量，放射免疫分析（RIA）法检测大鼠脑组织与脾脏中－β内啡肽水平。结果：电针能明显提高胶原诱导性关节炎大鼠的痛阈，对不同时期胶原免疫大鼠的痛阈有显著的即时升高效应，并能相应地增加大鼠脑组织和脾脏中β－内啡肽的含量。电针不仅能有效地减少造模大鼠的肢体累及率，更明显地降低大鼠的关节炎指数和血清中抗Ⅱ型胶原抗体水平。同时电针能显著抑制胶原诱导的大鼠脾组织中IL－1β的水平。结论：本研究说明，电针在对疼痛和免疫失调相兼疾病的治疗中不仅能镇痛，同时进行相应的免疫调节，针刺镇痛与免疫调节存在一定的相关性。     

原花青素对CIA大鼠足爪组织中NF-κB/p65蛋白和血清中某些促炎性细胞因子表达的影响

目的研究原花青素对Ⅱ型胶原诱导的类风湿性关节炎(CIA)大鼠足爪组织中核转录因子NF-κB/p65蛋白表达的抑制作用和血清中炎性细胞因子IL-1β、TNF-α等水平的影响。方法建立Ⅱ型胶原诱导的CIA大鼠模型,免疫组化法检测足爪组织中NF-κB/p65蛋白的表达,ELISA法检测CIA大鼠血清中IL-1β和TNF-α的含量。结果原花青素能剂量依赖性地下调NF-κB/p65蛋白的表达,降低CIA大鼠血清中TNF-α和IL-1β的水平。结论原花青素对CIA大鼠炎症的抑制作用可能与其下调NF-κB/p65蛋白表达、降低促炎性细胞因子TNF-α、IL-1β的水平有关。     

青藤碱对Ⅱ型胶原诱导关节炎大鼠滑膜炎症的抑制作用及其机理探讨

[目的]研究青藤碱对于胶原诱导型关节炎（CLA）大鼠滑膜及表达IL－6mRNA的影响。[方法]采用CIA大鼠动物模型，HE常规病理切片，观察青藤碱对滑膜的炎性浸润、巨噬A型细胞增生、纤维组织增生及原代培养滑膜细胞表达IL－6mRNA的影响。[结果]青藤碱可明显减轻滑膜的炎性浸润、巨噬A细胞和纤维组织增生，减少滑膜细胞IL－6mRNA的表达。[结论]青藤碱可以通过抑制滑膜细胞恶性增殖及IL－6基因的表达来阻断滑膜炎的进程。     

原花青素对CLA大鼠足爪组织中NF-κB／p6S蛋白和血清中某些促炎性细胞因子表达的影响

目的研究原花青素对Ⅱ型胶原诱导的类风湿性关节炎（CIA）大鼠足爪组织中核转录因子NF-κB／p65蛋白表达的抑制作用和血清中炎性细胞因子IL-1β、TNF-α等水平的影响。方法建立Ⅱ型胶原诱导的CIA大鼠模型，免疫组化法检测足爪组织中NF-κB／p65蛋白的表达，ELISA法检测CIA大鼠血清中IL-1β和TNF-α的含量。结果原花青素能剂量依赖性地下调NF-κB／p65蛋白的表达，降低CIA大鼠血清中TNF-α和IL-1β的水平。结论原花青素对CIA大鼠炎症的抑制作用可能与其下调NF—KB／p65蛋白表达、降低促炎性细胞因子TNF-α、IL-1β的水平有关。     

金雀异黄素对Ⅱ型胶原诱导型关节炎大鼠成纤维样滑膜细胞分泌白细胞介素1β和肿瘤坏死因子α的影响

目的：观察金雀异黄素（genistein,Gen）对Ⅱ型胶原诱导型关节炎（collagen-induced arthritis,CIA）大鼠膝关节成纤维样滑膜细胞（fibroblast-like synoviocyte,FLS）分泌炎症因子白细胞介素1β（interleukin-1β,IL-1β）和肿瘤坏死因子α（tumor necrosis factor-α,TNF-α）的影响。方法：采用Ⅱ型胶原注射建立CIA动物模型,用关节炎指数（arthritis index,AI）分析法评估CIA模型是否建立成功。胶原酶消化法分离培养原代FLS,用流式法检测滑膜细胞血管细胞黏附分子-1（vascular cell ad-hension molecule-1,VCAM-1）的表达。在FLS培养液中加入不同浓度的Gen,用酶联免疫吸附测定法检测FLS培养上清中IL-1β和TNF-α的含量。结果：Ⅱ型胶原致敏后3d,大鼠开始出现关节肿胀,AI逐渐增高,至造模后第3周最为明显,与空白对照组比较差异有统计学意义。大鼠滑膜细胞培养至第4代,检测其VCAM-1的表达高达85.5%,提示所培养的细胞为FLS,即B型滑膜细胞。不同浓度的Gen（100、200、400μmol/L）作用于FLS细胞后,细胞培养上清中TNF-α和IL-1β的分泌不同程度地受到抑制,且抑制作用呈剂量依赖性。结论：金雀异黄素能抑制FLS分泌TNF-α和IL-1β,且抑制作用呈剂量依赖性,这可能是其抗CIA大鼠滑膜炎的作用机制之一。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.