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1.
【目的】探讨不同碘营养摄人对哺乳期子代大鼠小脑浦肯野细胞蛋白-2的影响。【方法】按体质量将4~6周龄健康Wistar雌性大鼠随机分为4组:重度缺碘组、轻度缺碘组、正常碘组和碘过量组。每组大鼠食用低碘鼠粮,饮用含不同IK浓度的自来水,雄鼠按正常组条件饲养,8周后交配,取哺乳期14和28d的仔鼠作为研究对象。砷铈催化分光光度法检测仔鼠的尿碘水平,竞争结合放射免疫分析方法检测甲状腺激素水平,实时荧光定量PCR检测浦肯野细胞蛋白-2mRNA的表达水平,蛋白免疫印迹杂交和免疫组织化学方法检测其蛋白表达水平。【结果】2d龄的子鼠,在重度碘缺乏时,表现为低尿碘和甲状腺功能减退,血TF4水平明显低于正常组,浦肯野细胞蛋白-2的mRNA和蛋白表达也明显低于正常组(P〈0.05);轻度碘缺乏和碘过量时,TT4水平接近于正常组,浦肯野细胞蛋白-2的mRNA和蛋白表达与正常组相比,有轻度落后趋势,但无显著差异。【结论】哺乳期母鼠严重碘缺乏时,严重影响仔鼠小脑浦肯野细胞蛋白-2的表达,轻度碘缺乏和碘过量影响程度较低,提示哺乳期妇女碘营养状况不可忽视。  相似文献   

2.
目的探讨单纯碘过量对雌性大鼠血清甲状腺激素(T3、T4、FT3、FT4和TSH)水平的影响。方法选择成年雌性大鼠40只,随机分为4个组(每组10只):适碘组(NI)、高碘3个组(饮用水加入不同剂量的碘化钾(KI)),大鼠饲养3个月处死后取血离心检测血清甲状腺激素浓度水平。检测结果 EPI6.0双录入,分析用SPSS19.5统计软件。结果高碘组雌性大鼠血清T3、FT3、T4和FT4浓度与适碘组比较有所降低,但差异无统计学意义(P0.05),随着KIO3浓度的升高,各组血清T3、FT3、T4和FT4水平也随之降低;高碘组雌性大鼠血清TSH浓度与适碘组比较有所升高,差异有统计学意义(P0.05)。结论单纯的碘过量是甲状腺疾病的可能致病因素之一,所以因地制宜根据碘缺乏病的监测结果,及时调整盐碘浓度对于防治甲状腺疾病的发生具有重要意义。  相似文献   

3.
目的 探讨不同碘摄入水平对子代大鼠神经发育行为的影响。方法 断乳2周的SPF级雌性Wistar大鼠150只,按体质量随机分成5组,分别是碘缺乏1组(severe iodine deficiency,SID)、碘缺乏2组(mild iodine deficiency,MID)、适碘对照组(normal iodine,NI)、碘过量1组(mild iodine excess,MIE)和碘过量2组(severe iodine excess,SIE),各组饮水碘酸钾浓度依次为0、50、183、2283和11617μg/L,干预3个月,对子代进行Morris水迷宫实验、平衡木实验、钢丝夹持实验、转棒实验。结果 5个剂量组子代大鼠平衡木通过时间及在平衡木上发生打滑次数、在钢丝上的悬挂时间比较差异有统计学意义(F=2.640,χ2=11.873,F=3.178,P<0.05),均表现出适碘对照组子代大鼠具有较强的运动能力;定位巡航实验发现,第2天和第3天各剂量组子代大鼠逃避潜伏期时间均比NI组长(P<0.05)。空间探索实验显示,SID组和SIE组较NI组有效区域运动时间有所延长(P<0.05),各组均比NI组穿过平台的次数少(P<0.05)。结论 孕期不同程度的碘缺乏和碘过量均会损伤子代大鼠的学习记忆及运动能力。  相似文献   

4.
目的:研究富碘中药复方及碘过量对于碘缺乏NOD.H-2h4小鼠心、肾2型脱碘酶(D2)mRNA表达的影响。方法:选雌性8周龄NOD.H-2h4小鼠,给予双蒸水及低碘饲料喂养90d后制成碘缺乏自身免疫性甲状腺炎易感的甲状腺肿模型。随机分为正常对照组(NC)、低碘组(LI)、碘过量组(IE)(碘酸钾配制的含碘1900μg/L的去离子水)、富碘中药复方组(HIE)(海藻15g、昆布15g、海带7.5g,碘含量1900.36μg/L)。给予处理因素90d后处死。应用放射免疫RIA法测小鼠血清总T3(TT3)、总T4(TT4)。IRMA法测定小鼠血清促甲状腺激素(TSH)。光镜观察甲状腺组织细胞形态。应用实时定量RT-PCR法测定小鼠心、肾D2mRNA的表达情况。结果:给予处理因素90d后,各组间血清TT3,TT4及TSH值比较均未见明显差异(P>0.05)。NC、LI、IE与HIE组小鼠自身免疫性甲状腺炎的发生率分别为:2/10、0/10、9/10、3/10。小鼠心D2mRNA的表达,LI组(1.76±0.34)与NC组(1.02±0.25)相比有明显的升高趋势(P<0.01),IE组(1.42±0.33)的表达显著高于HIE组(0.76±0.32)的表达(P<0.05);小鼠肾D2mRNA的表达,IE组(4.07±0.88)较HIE组(1.91±0.62)升高趋势明显(P<0.01)。结论:摄碘量相同的情况下,单纯碘过量可致NOD.H-2h4小鼠心、肾D2表达增加,富碘中药复方亦可致小鼠肾D2表达增加,小鼠心中未见此变化。  相似文献   

5.
目的 探讨母体壬基酚暴露对后代学习记忆的影响.方法 受试雌鼠与雄鼠同笼,见阴栓确定受孕后分组试验:将SD大鼠随机分为4组(对照组、50mg/kg、100mg/kg、200mg/kg壬基酚组),每组6~7只,妊娠第9~15天灌胃壬基酚.用Morris水迷宫和跳台的方法观察壬基酚对仔鼠学习记忆的影响;海马组织透射电镜观察其病理变化.结果 NP200mg/kg组仔鼠水迷宫中逃避潜伏期延长(61.14±5.92)s,错误次数增加(4.57±1.13)次;跳台实验中反应时间延长(37.5±6.3)s,步下潜伏期缩短(97.8±11.0)s,错误次数增加(3.0±1.4)次,阴性对照组分别为(35.85±4.29)s,(2.57±0.97)次,(27.1±3.8)s,(172.0±89.2)s,(0.9±0.7)次)],与对照组比较,差异具有显著性(P<0.05).NP200mg/kg剂量组仔鼠海马组织电镜下见线粒体肿胀呈空泡样变,染色质浓缩成块状聚集在核周围.结论 壬基酚官内暴露可导致仔鼠空间学习记忆障碍和海马超微结构改变.  相似文献   

6.
目的 探讨慢性碘超足量和碘过量对Wistar大鼠甲状腺细胞死亡和细胞周期的影响.方法 将Wistar大鼠按日摄碘量分为对照组(碘适量组,4μg/d)、1.5倍组(碘超足量组,6μg/d)、3倍组(3倍碘过量组,12μg/d)和6倍组(6倍碘过量组,24μg/d).各组大鼠分别于实验0、1、2、4、8个月处死,采用砷铈催化分光光度法测定尿碘浓度,应用碘化丙锭染色流式细胞术测定细胞死亡率和细胞周期.结果 3倍组和6倍组在4个月和8个月时,甲状腺细胞死亡率升高(17.4%vs 9.8%,P<0.05),S期细胞比例升高(5%~6%vs 3%,P<0.05),G_0G_1期细胞数显著下降(64%~67%vs 80%,P<0.05).尿碘浓度与细胞死亡率、S%显著正相关(r=0.673,r=0.505,P均<0.01),与G_0G_1%显著负相关(r=-0.639,P<0.01).结论 慢性碘过量增加甲状腺细胞的死亡率,并影响G_0G_1期和S期细胞比例.  相似文献   

7.
目的:对不同碘过量水平大鼠的脾脏进行形态学研究,探讨碘过量对机体免疫功能的影响。方法:断乳1个月Wistar大鼠24只,雌雄各半,随机分为4组:适碘组(NI)、10倍碘组(10 HI)、50倍碘组(50 HI)和100倍碘组(100HI)。给以不同浓度碘化钾水喂养3个月后,处死取脾脏,对脾脏进行光镜结构观察,并对大鼠脾脏脾小体、脾小体生发中心、动脉周鞘和边缘区的体密度进行测量。结果:低水平碘过量(10 HI)大鼠脾脏未见明显形态学改变,高水平碘过量(50 HI和100 HI)大鼠脾脏脾小体、脾小体生发中心、动脉周鞘和边缘区的体密度明显增高,即呈现功能活跃的表现。结论:高水平碘过量可引起大鼠免疫功能增强。  相似文献   

8.
目的探讨高碘高氟对儿童甲状腺肿与氟斑牙的影响及特点。方法对黄泛区水源性高碘高氟和高氟适碘区(研究组)、低氟适碘区(对照组)全部8~10岁儿童共3053名进行甲状腺肿、氟斑牙检测。结果甲状腺肿检出率:高碘高氟组(15.41%)高于高氟适碘组(10.02%),2=14.678,P<0.01)。两组均高于适碘低氟组(5.19%),分别为2=48.729,P<0.01;2=15.599,P<0.01。氟斑牙检出率:研究组间高碘高氟组(52.84%)与高氟适碘组(49.05%)无差异,2=3.155,P>0.05,但均高于对照组(5.68%),分别为2=463.237,P<0.01;2=432.845,P<0.01。结论高氟高碘对甲状腺肿的发生具有叠加、协同效应;对氟斑牙的形成,高碘与高氟无叠加协同效应。  相似文献   

9.
地方克汀病的病因和发病机制至今尚未完全阐明,一般认为是由于胚胎期缺碘、甲状腺激素不足使中枢神经系统、骨骼系统等发育障碍的结果,但在实验室中用缺碘方法复制地方克汀病动物模型还未获完全成功。我们曾在大鼠中进行实验,给已怀孕的母鼠饲以低碘饮食、并于仔鼠出生后仍继续饲以低碘饮食,观察到胚胎发育后期及新生期缺碘对出生30天后仔鼠的中枢神经系统功能和形态均有一定影响,但不十分严重。本研究在原有基础上进行实验,目的在于阐明整个胚胎期缺碘对脑发育的影响。材料和方法一、动物分组及处理采用同系Wistar大鼠,按过去胚胎期和哺乳期缺碘实验的条件进行。分两  相似文献   

10.
高胆固醇血症大鼠心室肌细胞动作电位时程的变化   总被引:1,自引:0,他引:1  
目的:观察高胆固醇血症大鼠心室肌细胞动作电位(APD)时程的变化.方法:应用全细胞膜片钳技术记录高胆固醇大鼠心室肌细胞动作电位的变化.结果:高胆固醇血症组血清TC含量从1.34 ± 0.22mmol/L增至7.58±1.55mmol/L(P<0.01 n=5).高胆固醇血症大鼠单个心室肌细胞APD50( 116.2±15.4ms)明显高于正常组大鼠APD5070.8±18.1ms (n=5,p<0.01), 高胆固醇血症组APD90(144.0±16.50ms)明显高于正常组(95.1±16.2ms)(n=5, p<0.01);结论:高胆固醇血症大鼠动作电位时程明显延长,动作电位时程延长易发生早后除极和迟后除极.  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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