高血压患者红细胞Ca2+摄入的特点及对非洛地平的反应

目的：探讨原发性高血压（ＥＨ）和继发性高血压（ＳＨ）细胞膜对Ｃａ＾２＋调节的特点及钙拮抗剂对其影响。方法：３８例高血压病人分为ＥＨ组（２０例）和ＳＨ组（１８例），ＳＨ组又为肾血管性（ＲＨ，５例）、肾实质性（ＰＨ，７例）和内分泌性（ＥＨＴ，６例）高血压３个亚组。分别设对照组。用液态闪烁计数法测定红细胞＾４５Ｃａ＾２＋摄入；采用放射免疫分析法测定肾素活性（ＰＲＡ）、血管紧张素Ⅱ（ＡⅡ）。ＥＨ组和ＳＨ组     

原发性高血压患者红细胞[Ca2+]i浓度变化及影响因素

目的观察原发性高血压患者红细胞[Ca2+]i,多巴胺β羟化酶及ATP含量变化并分析其结果. 方法测定35例高血压患者的红细胞[Ca2+]i、ATP、血清多巴胺β羟化酶活性、血糖及血浆胰岛素含量,并以30例健康成年人为对照. 结果高血压患者的红细胞[Ca2+]i、ATP、多巴胺β羟化酶均明显高于对照组(P<0.05,P<0.01),但血糖与胰岛素未见明显变化. 结论高血压患者血清多巴胺β羟化酶活性增强伴随ATP与[Ca2+]i升高.     

经穴Ca2+浓度的分布及针刺对Ca2+影响的实验研究

在技术方法方面,研制成功了可用于在体测量的Ca²⁺选择性针型电极,可对活体细胞外Ca²⁺进行连续动态的在体测定,实时提供Ca²⁺活度变化的信息;改良了同轴复合式推挽灌流管,以平行并外式推挽灌流管代替之,可刺入外围组织观察细胞外液中成分。     

Wnt/Ca2+信号通路的生理及病理生理学研究进展

<正>1982年,美国加利福尼亚大学NUSSE等^[1]将分别来自果蝇的Wingless蛋白和小鼠的Int蛋白的具有同源性、富含半胱氨酸残基的2种分泌型糖蛋白合称为Wnt蛋白。Wnt信号通路的启动主要依靠Wnt配体蛋白、Wnt受体及相关配件。目前,Wnt配体蛋白在人类和哺乳动物中已发现有19种,Wnt受体主要由卷曲蛋白(Frizzled)家族和非Frizzled家族类蛋白构成。     

白虎汤加减方中Ca2+浓度测定

目的：测定白虎汤加减方中Ca²⁺浓度，为中国剂改提供一定依据。办法：在白虎汤加减方中，改变石膏用量，用EDTA滴定法测其水煎液Ca²⁺浓度。结果：表明Ca²⁺浓度与方剂组成有关系。4.结论：在方剂的剂量有一定的根据，临床和剂改中不应轻易变动。     

The effects of local anesthetics on intracellular Ca2+ release from ryanodine-sensitive Ca2+ stores in gerbil hippocampal neurons

Objective To examine the effects of procaine and lidocaine on intracellular Ca(2+) release from sarcoplasmic reticulum ryanodine-sensitive Ca(2+) stores. Methods The experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils. Levels of intracellular Ca(2+) concentration in the slices were measured by microfluorometry. The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds. Then, the medium was switched to physiological medium. After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min. Following incubation, the slice was superfused with physiological medium until the end of the experiment. The effects of procaine and lidocanin (100 μmol/L) on caffeine-evoked Ca(2+) release were evaluated by adding them to the medium after high K(+) medium perfusion. Results Caffeine induced a marked increase in intracellular Ca(2+) concentration which was then decreased 12% upon the addition of procaine (P&lt;0.05); however, lidocaine, did not induce a similar inhibitory reaction.Conclusion Procaine inhibits ryanodine-receptor mediated Ca(2+) release from intracellular Ca(2+) stores, while lidocaine may inhibit Ca(2+) release through other mechanisms.     

ATP2A2通过钙离子浓度变化参与肿瘤发生机制的研究进展

ATP2A2是ATP2As基因家族的成员,编码肌浆(内质)网钙转运ATP酶中的一种,即SERCA2b。由于该酶的主要功能为将钙离子从胞质转运至肌浆(内质)网内,因而它在控制肿瘤生长、分化、血管生成、转移和凋亡的钙离子相关信号通路中发挥着重要作用。近期研究已在一些类型的肿瘤中明确了ATP2A2表达水平的具体变化,在探究ATP2A2是如何参与肿瘤形成以及它是否可作为肿瘤标记物和治疗靶点方面迈出了第一步。本文对ATP2A2参与肿瘤发生的可能机制的相关研究进行了总结,认为其表达异常可引起细胞胞质内和内质网间钙离子稳态的破坏,从而造成细胞的恶性增殖并促进肿瘤的迁移和血管形成等。本文还对该基因的研究和应用前景进行了展望。     

Changes in Ca2+-ATPase in a guinea pig endolymphatic hydrops model

ObjectiveTo investigate the localization of Ca(2+)-ATPase (Ca(2+) pump) in the cochlear and its change afterendolymphatic hydrops, and to study the relationship between compound action potential (CAP) threshold and the Ca(2+)-ATPase activety.MethodsThe left endolymphatic sac was ablated to induce endolymphatic hydrops in fourteen healthy guinea pigs with normal action potential thresholds measured after asliver ball electrode placed on the round window.Ca(2+)-ATPase activity was studied cytochemically using a lead citrate reaction in control and hydropic ears.The reaction product was lead phosphate particles as an expression of Ca(2+) -ATPase activity, observed with an eletron microscope.ResultsCa(2+)-ATPase activity is mainly found on the endolymphatic surface of Reissner’s membrane, the stereocilia and cuticular plate of inner and outer hair cells, and along the infolded plasma membrane ofstrial marginal cells.CAP thresholds of filtered click are increased and Ca(2+)-ATPase activity significantly decreased after endolymphatic hydrops in the mentioned locations.ConclusionsCAP thresholds are increased and Ca(2+)-ATPase activity are significantly decreased in the cochlea after endolymphatic hydrops.These results suggest that there is a negative correlation between them.      

2-甲基-3-羟基蒽醌诱导人乳腺癌MCF-7细胞凋亡的机制

为探讨2-甲基-3-羟基蒽醌抗肿瘤作用及其机制,本研究采用锥虫蓝法检测细胞活力,流式细胞仪检测细胞周期变化、细胞凋亡率、线粒体膜电位及细胞内游离钙的变化,Western blot方法检测凋亡相关蛋白caspase-4、caspase-7、caspase-9、Bcl-2、Bax、JNK、细胞色素C的表达。结果发现：2-甲基-3-羟基蒽醌时间依赖性地抑制乳腺癌细胞的生长,升高细胞内游离钙含量,降低线粒体膜电位并诱导其凋亡;药物上调Bax并下调Bcl-2蛋白的表达;诱导caspase-4、caspase-7、caspase-9、calpain的活化及细胞色素C的释放。结果提示2-甲基-3-羟基蒽醌可能通过Ca²⁺/calpain/caspase-4途径诱导人乳腺癌MCF-7细胞凋亡。     

葛根素参与的可注射钙离子敏感型水凝胶生物相容性研究

[目的] 将用于关节炎治疗的钙离子敏感型葛根素-海藻酸钙可注射水凝胶进行体内生物相容性评价研究。[方法] 制备不同处方配比的葛根素-海藻酸钙水凝胶,通过大鼠皮下注射埋植实验,考察葛根素-海藻酸钙水凝胶的体内降解情况;同时通过实时定量逆转录-聚合酶链反应（RT-PCR）和酶联免疫吸附（ELISA）分别测定注射水凝胶周围组织炎性相关因子白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的基因表达和蛋白分泌变化;通过组织切片苏木精-伊红（HE）染色观察不同埋植时间点水凝胶周围组织病理变化。[结果] 不同观察时间点各实验组凝胶直径无下降趋势,凝胶体内降解速率缓慢;与假手术组比较,葛根素-海藻酸钙凝胶周围组织中炎性因子IL-1β、IL-6和TNF-α蛋白浓度显著降低,而IL-1β、IL-6和TNF-α的mRNA表达无显著变化;组织切片HE染色结果表明在皮下结缔组织层产生短期物理损伤炎症反应,长期埋植后葛根素-海藻酸钙凝胶周围组织无炎症反应,具有良好的生物相容性。[结论] 研究中构建的可注射葛根素-海藻酸钙凝胶,体内降解缓慢,生物相容性良好,有望成为治疗骨关节炎的良好药物及细胞载体。     

葛根素参与的可注射钙离子敏感凝胶构建和性质研究

[目的]构建一种葛根素(Pu)参与的海藻酸钙水凝胶,具有可注射性和钙离子敏感性,可用于控释药物或负载间充质干细胞,用于骨关节炎治疗。[方法]以海藻酸钠(SA)、氯化钙(CaCl_2)和Pu为原料制备不同处方凝胶,黏度计实验考察处方原料对凝胶黏度的影响,通过流变学方法筛选最佳处方,并考察Pu对凝胶强度的影响,观察冻干凝胶的微观结构,通过溶胀实验、体外降解实验和药物释放实验考察凝胶溶胀率、体外稳定性和药物缓释性能。[结果]通过物理搅拌得到均一透明凝胶,处方中原料对凝胶强度影响大小顺序为CaCl_2SAPu,筛选最佳处方为0.2%Pu+0.6%SA+6 mmol/L CaCl_2,扫描电微镜下凝胶呈现三维网孔结构,表征实验表明Pu对凝胶强度有促进作用,Pu使凝胶溶胀率由75倍减小至45倍,体外初始降解时间由12 d增加至24 d,该凝胶对粉防己碱具有良好的缓释作用。[结论]Pu参与海藻酸钙凝胶构建,增加胶凝强度并改善凝胶溶胀和体外降解性质,该凝胶强度适宜并呈三维网孔结构,对粉防己碱有缓释作用,是良好的可注射型药物或干细胞载体用于骨关节炎的治疗。