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1.
目的建立反相高效液相色谱法测定人血清中茶碱浓度。方法血清样品加入咖啡因为内标,10%三氯醋酸去蛋白,采用Ultimate XB-C18(250 mm×4.6 mm,5μm)色谱柱分离,流动相为甲醇∶水=30∶70,流速为1.0 mL/min,检测波长为273 nm,柱温为30℃。结果茶碱在1.76~44.00 mg/L浓度范围线性关系良好,r=0.999 6,血清最低检测限为0.44 mg/L,日内精密度和日间精密度良好,回收率较高。结论本法操作简便,结果准确可靠。可满足临床快速监测的需要。  相似文献   

2.
目的:建立测定大鼠血浆多索茶碱血药浓度的高效毛细管电泳法.方法:样品用体积分数30%三氯醋酸沉淀蛋白,电解缓冲液为25 mmol/L磷酸盐缓冲液-50 mmol/L SDS(体积比为20:80,pH=7.0),分离通道为50cm×50 μm未涂层毛细管,紫外检测波长为273 nm,分离电压12 kV,采用峰面积内标法定量.结果:采用本法时,多索茶碱血浆浓度在2.5~80.0 mg/L范围内线性关系良好,标准曲线回归方程为y=-8.40×10-3 0.13X(r=0.998 1,P<0.05),方法回收率在允许范围内,日内和日间变异分别小于3.4%和7.5%.结论:本法稳定可靠、操作简单、分析速度快、分离效果好.  相似文献   

3.
目的:建立同时测定人血清中茶碱和多索茶碱浓度的反相高效液相色谱方法。方法:采用内标法,咖啡因为内标,分析柱为WondaSilCl8,流动相为甲醇-水-三乙胺-冰醋酸(35:65:0.01:0.01),流速为1.0mL/min,柱温:30℃,检测波长为273nm;血清样品经12%高氯酸直接沉淀蛋白离心后取样进行色谱分析。结果:样品与内标的峰面积比(R)同样品浓度(C)间线性均良好,线性范围均在2.5-40mg/L,回归方程分别为:茶碱C=3.746R+0.4436,r=0.9997,多索茶碱C=10.24R-4.506,r=0.9999;平均回收率为:茶碱99.89%(RSD=1.07%,n=5),多索茶碱99.60%(RSD=0.81%,n=5)。结论:该方法简单方便,快速,准确  相似文献   

4.
目的 建立能够同时测多索茶碱、苯妥英钠、卡马西平和苯巴比妥这4种药血药浓度的反相高效液相色谱法.方法 采用日本岛津LC-20AT高效液相色谱仪,以ODS-C18柱(150 mm×4.6 mm×5μm)为色谱柱,流动相为甲醇-水(50:50),流速为0.8 mL/min,柱温为20℃,检测波长210 nm,进样量20 μL.结果 多索茶碱、苯妥英钠、苯巴比妥和卡马西平线性关系良好,线性范围分别为2.5~30.0 μg/mL(r=0.999 5),2.5~30.0 μg/mL(r=0.999 4),2.5~50.0 μg/mL(r=0.999 7),1.0~20.0 μg/mL(r=0.999 7),方法回收率均>95%,日间的精密度和日内的精密度均<6%(n=5).结论 该方法迅速、准确、灵敏,4种药物能够被良好分离,且没有出现被测药物之间相互干扰,可用于临床监测血药浓度.  相似文献   

5.
HPLC法测定氨茶碱注射液中茶碱的含量   总被引:1,自引:0,他引:1  
目的:建立HPLC法测定氨茶碱注射液中茶碱的含量。方法:采用Kromonsil C18(4.6mm×150mm,5μm)色谱柱;流动相:甲醇-0.01mol/LKH2PO4(30:70)(三乙胺调节pH=6.5);流速:1.0ml/min;检测波长:271nm;结果:茶碱在9.98~199.68μg/ml范围内线性关系良好(r=0.9999),高、中、低3种浓度平均加样回收率为98.93%,RSD=1.47%(n=9)。结论:本法简便、准确,分离效果佳,可用于测定氨茶碱注射液中茶碱的含量。  相似文献   

6.
介绍高效液相色谱测定血清茶碱浓度的方法。血清内茶碱用氯仿/异丙醇(95/5,含内标剂β—羟乙基茶碱)提取,蒸干,用甲醇重溶后在Shim—pack CLC-ODS柱(6mm×15cm)上,以醋酸盐缓冲液(10mmol/L,pH4.0)/乙腈/甲醇(83/9/8)作流动相,流速1ml/分进行分析。从茶碱和内标剂峰面积比定量。本法的分析回收率平均为100.1%(范围95,8%~106.8%)。批内CV分别为2.6%(x=4.125mg/L)、1.7%(x=8.73mg/L)和1.0%(x=19.967mg/L),批间CV2.8%(x=8.91mg/L)。血清茶碱浓度在0~40mg/L范围内呈线性,回归方程y=0.0483x-0.0053。检测下限为0.3mg/L(信号/噪声=3)。常用解热镇痛药和常用镇静药无干扰。  相似文献   

7.
目的 对比多索荼碱与氨茶碱治疗支气管哮喘的临床疗效.方法 选取我院2014年1月-2015年收治的78例支气管哮喘患者,随机分成2组,A组用多索茶碱治疗,B组用氨茶碱治疗,治疗1周后,比较2组患者的治疗有效率.结果 多索茶碱治疗组的有效率为94.9%,氨荼碱治疗组的有效率为71.8%,组问差异具有统计学意义(P<0.05).结论 多索茶碱治疗支气管哮喘的疗效高于氨荼碱.  相似文献   

8.
HPLC法测定茶碱血药浓度及其临床应用   总被引:2,自引:1,他引:2  
目的 建立血清中茶碱的HPLC测定方法 ,开展茶碱血药浓度临床监测.方法 采用15%的高氯酸处理血样,色谱柱为Shim-pack VP-ODS(150 x4.6mm,5 μm),流动相为甲醇:水=28:72,检测波长为274 nm.流速1mL/min,内标为咖啡因.结果 茶碱标准曲线回归方程为Y=1.12742X-0.0173523,r=0.9997(n=5),线性范围为1~40 μg/mL.茶碱5、15、30 μg/mL提取回收率分别为75.1%、73.9%、74.5%,方法 回收率为102.3%、99.0%、99.8%,日内RSD(相对标准差)分别为5.8%、2.8%、3.5%,日问RSD分别为4.1%、3.4%、2.6%.结论 本测定方法 简便、快速、准确,适合茶碱临床血药浓度监测.  相似文献   

9.
目的:建立以高效液相色谱法测定生附子中乌头碱及次乌头碱的含量的方法。方法:采用色谱柱为:Welchrom-C18,5.L,4.6×250mm,流动相:甲醇:水:三乙胺(72:28:0.037),流速:1.0mL/min,柱温30℃,检测波长分别为235、233nm。结果:乌头碱在0.00376~0.0188mg.mL-1范围内线性关系良好,r=0.9999,平均加样回收率为96.10%;RSD=1.06%;次乌头碱在0.01818~0.04242mg范围内线性关系良好,r=0.9999;100%、120%、80%加样平均回收率为100.5%,RSD值为1.53%。结论:该方法简便、准确、重复性好,可用于附子中乌头碱及次乌头碱的含量测定。  相似文献   

10.
HPLC法测定生物样品中茶碱的含量   总被引:1,自引:0,他引:1  
目的探讨用反相高效液相色谱法测定家兔血清中茶碱的浓度。方法采用C18柱,流动相为甲醇-水(35:65),检测波长275nm,柱温为室温,测定茶碱的血药浓度。血清中的茶碱用氯仿-异丙醇(95:5)提取。结果茶碱浓度在14.93~51.6μg/ml范围内与峰面积线性关系良好,线性方程为Y=6441.5x-76922,r=0.9947。结论用本法对6只家兔进行了血药浓度监测。该法取样量少,快速、灵敏、准确,可用于常规茶碱血药浓度的监测和进行药代动力学研究。  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

14.
FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…  相似文献   

15.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

16.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

17.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

18.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

19.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

20.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

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