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1.
 [目的]探讨内皮祖细胞修复动脉内皮损伤的作用.[方法]建立大鼠颈总动脉内皮损伤模型,16只大鼠随机等分为内皮祖细胞移植组和对照组.体外诱导大鼠外周血单个核细胞为内皮祖细胞.在动脉损伤后,将5-溴脱氧尿苷标记的内皮祖细胞注入损伤动脉段,14 d后取损伤动脉段和对侧正常动脉段.采用病理学方法、免疫组织化学和图像分析技术评价内皮祖细胞的修复效果.[结果]内皮祖细胞移植组的损伤动脉段5-溴脱氧尿苷免疫组化为阳性.内皮祖细胞移植组损伤动脉段的新生内膜厚度较对照组明显减轻(43.5±5.5)μm vs(90.7±12.7)μm,P<0.05;内皮祖细胞移植组的再内皮化程度明显高于对照组77.8%±0.1%vs52.2%±0.1%,P<0.05.[结论]内皮祖细胞具有修复动脉内皮细胞损伤的作用,内皮祖细胞可作为心血管疾病细胞移植疗法的种子细胞.  相似文献   

2.
摘要: 目的:观察针刺太冲穴对自发性高血压大鼠(SHR)收缩压、血浆内皮素(ET-1)及血清NO含量的影响,以对其降压机制作初步探讨。方法:选用9周龄SHR28只,随机分为模型组、针刺太冲、针刺冲阳和针刺非经穴组(均n=7),并以同体重正常血压SD大鼠(n=7)作为正常组。经过连续针刺治疗7天后,测量收缩压,并测定血浆ET-1和血清NO的水平。结果:(1)模型组血压显著高于正常组(P<0.01)。经针刺治疗7天后,针刺太冲组大鼠收缩压显著低于模型组[(140.81±4.47)mmHg vs (166.58±9.05)mmHg,P<0.01],但仍高于正常组[(140.81±4.47)mmHg vs (99.17±9.24)mmHg,P<0.01]。(2)针刺太冲组大鼠针刺第7天收缩压显著低于第1天[(140.81±4.47)mmHg vs (154. 56±10.59)mmHg,P<0.01]。(3)针刺太冲能显著降低SHR血浆ET-1水平[(93.19±4.39) pg/mL vs (105.82±6.82)pg/mL,P<0.05]和升高血清NO的水平[(45.01±8.1)umol/L vs (28.28±4.86)umol/L,P<0.01]。结论 针刺太冲穴能降低SHR收缩压,但未能使其收缩压恢复至正常水平,其降压机制可能与降低SHR血浆ET-1和升高血清NO的含量有关。  相似文献   

3.
目的观察同种异体心肌细胞移植对心肌梗死大鼠心肌的修复作用和心功能的影响。方法选取110只雄性SD大鼠用于制作心肌梗死模型,术后2周共存活53只,死亡57只。从53只建模成功的SD大鼠中随机选取30只分为3组(每组10只):心肌细胞移植组(A组)、培养液基质注射组(B组)和单纯心肌梗死组(C组)。A组将原代培养的乳鼠心肌细胞移植到心肌梗死区和梗死边缘区;B组采用与A组同样方法在心肌梗死区和梗死边缘区注射细胞培养液基质;C组不做任何处理。2周后观察A组移植细胞存活情况。采用Langendorff装置检测各组左心室收缩压(LVSP)、左心室舒张末压(LVEDP)、左心室内压最大上升速率(+dp/dtmax)、左心室内压最大下降速率(-dp/dtmax)和左心室做功(LVW)。结果A组与B组及C组相比,LVSP分别从(47.9±4.6)mmHg和(47.6±3.9)mmHg升高到(52.0±3.0)mmHg(P〈0.05或P〈0.01);LVEDP分别从(18.8±2.7)mmHg和(24.7±5.8)mmHg降低到(15.8±1.5)mmHg(P〈0.05或P〈0.01);+dp/dtmax分别从(1 900±198)mmHg/s和(1 737±347)mmHg/s升高到(2 260±337)mmHg/s(P〈0.05)。3组间-dp/dtmax和LVW比较差异无显著性(P〉0.05)。A组大鼠的心肌中均发现移入的乳鼠心肌细胞,经5-溴脱氧尿核苷(BrdU)标记的免疫组化染色和抗心肌肌钙蛋白I抗体(anti-cTn I)标记的免疫组化染色证明移入的细胞是心肌细胞。结论异体移植的乳鼠心肌细胞可在心肌梗死区存活,并能改善心功能,尤其是改善左心室收缩功能。  相似文献   

4.
目的 探讨大鼠急性心肌梗死(AMI)后心功能变化和病理演变规律及其对心肌梗死干细胞移植治疗的影响.方法 雄性Wistar大鼠40只随机分为假手术0周组(SH 0周组,n=1 0)、MI 2周组(MI 2周组,n=10)、MI 4周组(MI 4周组,n=1 0)、MI 8周组(MI 8周组,n=10).结扎冠状动脉左前降支制作大鼠急性MI模型,分别于术后观察期满,用超声评价心脏结构和左心导管检测血流动力学改变,并取心脏组织行HE染色,观察心肌病理改变.假手术组仅开胸并在左前降支下过线.结果 MI2周后与假手术0周组相比,大鼠左心室舒张末压明显增高(LVEDP 1 9.75±7.1 8 mmHg v s 2.1 0±1.26 mmHg,P<0.01),左心室内压力最大上升速率明显减慢( dp/dtmax 4.11±0.74 mmHg/ms vs 6.20±0.70 mmHg/ms,P<0.01),最大下降速率亦显著减慢(-dp/dtmax 3.54±0.55 mmHg/ms v s 5.82±0.54 mmHg/ms,P<0.01).MI 4周时上述改变更明显,但MI 8周时心功能未进一步恶化.超声见心脏结构变化与血流动力学改变相一致.HE染色观察梗死后12 h梗死区弥漫的中性多形核细胞浸润,在2周时达到高峰,4周后基本消退.结论本研究显示大鼠梗死后其炎症反应高峰2周时出现,4周后基本完成梗死后修复过程,其演变规律可能对AMI干细胞治疗效果有影响.  相似文献   

5.
[目的]探讨急性心肌梗死早期溶栓治疗效果与QT间期离散度(QTd)的关系.[方法]226例急性心肌梗死患者分为溶栓成功组(63例);溶栓失败组(52例);非溶栓组(111例).所有研究对象分别在人院当时(平均7.1h±1.6h),发病后第2天、第1周末及第2周末记录标准12导联心电图.QT间期的测量从QRS波起点至T波终点,同一导联测量3个心动周期,取其平均值.不同导联最大QT值减去最小QT值为QTd值.[结果]3组QTd入院当时无差异(P>0.05),但起病后第2日、第1周末及第2周末溶栓成功组QTd[分别为(48±17)ms,(43±15)ms,(36±14)ms]较溶栓失败组[分别为(65±18)ms,(55±16)ms,(47±15)ms]和非溶栓组[分别为(67±16)ms,(54±14)ms,(49±13)ms]小,P<0.01;后2组比较,P>0.05.[结论]急性心肌梗死成功的早期溶栓治疗能使QTd值减小,提示急性心肌梗死早期梗死相关冠状动脉再通能提高心肌电稳定性.  相似文献   

6.
 目的 探讨骨髓单个核细胞植入对心肌梗死后心脏功能的影响。 方法 以冷冻损伤的方法建立新西兰种家兔心肌梗死模型,分为骨髓单个核细胞组(mononuclear bone marrow cells,MN-BMCs组)和对照组,各8只。4周后, MN-BMCs组中将提取分离的MN-BMCs,植入心肌梗死及其边缘区,对照组于心肌梗死及其边缘区注射相应体积的IMDM完全培养液。以超声心动图分析细胞植入前后心脏功能改善情况。 结果 MN-BMCs植入心肌梗死及其边缘区可以改善局部及整体心脏功能:细胞移植4周后两组之间比较,MN-BMCs组局部心脏功能明显优于对照组,表现为MN-BMCs组比对照组心肌梗死区室壁厚度增加,(4.81±0.55)mm vs 3.94±0.54)mm,P<0.05;收缩期和舒张期心肌梗死区室壁运动速度明显增加,分别为(8.20±0.77)cm/s vs 7.00±0.56)cm/s,P<0.05,(8.51±0.93) cm/s vs (6.75 ±0.57)cm/s,P<0.05。细胞移植4周后,MN-BMCs组整体心脏功能亦优于对照组,表现为MN-BMCs组比对照组心腔减小,LVDD分别为 (14.89±1.36)mm vs (16.24±1.31)mm,P<0.05,LVSD分别为(10.48±1.32)mm vs (12.29±1.48)mm,P<0.05;MN-BMCs组左心室收缩功能明显改善,左室射血分数及缩短分数提高,LVEF分别为(65.25±5.73)% vs (57.00±5.81)%,P<0.05,LVFS分别为 (30.25±3.73)% vs (24.88±3.95)%,P<0.05。 结论 骨髓单个核细胞植入心肌梗死及其边缘区可以改善心肌梗死后心脏功能。  相似文献   

7.
目的 探讨诱导多能干细胞来源的心肌细胞用于心肌梗死大鼠模型心肌修复的有效性,并阐明其改善心脏功能的机制。方法 体外培养诱导多能干细胞并诱导分化为心肌细胞;心肌梗死模型SD大鼠随机分为心肌梗死组和细胞移植两组,正常SD大鼠为对照组,每组10只。建模成功后,心肌梗死组和细胞移植组分别在心肌梗死边界附近的3个点注射30 μL的生理盐水和分化心肌细胞,对照组行开胸手术,不结扎冠状动脉;在第7、14和28天时通过心脏彩超和有创血流动力学测量心功能;第28天时RT-PCR检测组织衰老指标,Masson染色法评估梗死面积的大小和免疫组化检测梗死边缘区毛细血管密度。结果 28天时,与心肌梗死组相比,细胞移植组左室射血分数(LVEF)、左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)、左室舒张末期压力(LVEDP)及最大正负左室内压力变化速率(±dP/dt)明显改善(均P<0.05);细胞移植组的梗死面积明显小于心肌梗死组,而毛细血管生成明显多于心肌梗死组(P<0.05);心肌梗死组的基质金属蛋白酶-3(MMP-3)、内皮细胞型纤溶酶原激活物抑制因子(PAI-1)和白细胞介素-6(IL-6)的mRNA表达量明显升高(P<0.05)。结论 移植诱导多能干细胞分化的心肌细胞可通过旁分泌作用改善心肌梗死大鼠模型的心脏功能。  相似文献   

8.
大鼠骨髓单个核细胞移植治疗心肌梗死   总被引:2,自引:2,他引:0  
目的:探讨大鼠骨髓单个核细胞(mononuclear bone marrow cells,MBMCs)移植方法对梗死心肌的治疗效果.方法:无菌条件下取成年雄性近交系Wistar大鼠股骨和胫骨,常规抽取骨髓,分离制备MBMCs悬液.将成年雄性近交系Wistar大鼠46只随机分为空白对照组(组Ⅰ,n=10)、梗死对照组(组Ⅱ,n=18)、细胞移植组(组Ⅲ,n=18).用结扎冠状动脉左前降支的方法建立大鼠心肌梗死模型,在建模后2周分别将10μl MBMCs(6×106)悬液和等量磷酸盐缓冲液(PBS)植入组Ⅱ和组Ⅲ的梗死周边区.用心脏超声观察细胞移植后4周的心脏功能改变,用免疫荧光和免疫组化及电镜观察移植细胞在心肌梗死区及梗死周边区的存活、增殖和分化情况.结果:MBMCs移植4周后,移植组超声检查左室功能重建指标如左室射血分数(EF)、左室短轴缩短率(FS)、左室前壁厚度(LVAWT)均较梗死对照组改善明显(P<0.05);移植组血流动力学检查指标如左室收缩压(LVSP)、左室舒张末压(LVEDP)和左室舒张压力时间变化最大速率(±dp/dtmax)也优于梗死对照组(P<0.05).MBMCs移植6周后,大鼠心肌梗死区有移植细胞存活、增殖,并分化为有心肌细胞特征的细胞.结论:实验证实用MBMCs移植治疗心肌梗死,可以明显改善梗死后心脏功能.这一效果可能与MBMCs移植相关的心肌再生和新生血管形成有关.  相似文献   

9.
唐洁  苏彬  陈涛  米杰  张健  齐向前 《武警医学院学报》2013,(11):957-960,F0003
【目的】观察急性心肌梗死犬经冠脉移植自体骨髓单个核细胞后对心功能及血管生成的影响。【方法】结扎冠状动脉前降支建立犬急性心肌梗死模型,CM—DiI标记体外分离得到的骨髓单个核细胞bonemarrowmononuclearcell,BM—MNCs),分为移植组(n=10)和对照组(n=6),于心肌梗死后2h分别经冠状动脉内移植骨髓单个核细胞和生理盐水,冠状动脉结扎后2h及6周时分别测定血流动力学指标(心排m量、左心室收缩压、左心室舒张末压)。移植后6周,观察CM—Dil标记的BM—MNCs在心肌内的分布,检测心肌组织不同部位的毛细血管密度。【结果】经冠脉自体骨髓单个核细胞移植后6周,梗死边缘区和梗死中心区均可见CM—DiI标记的细胞;血流动力学指标显示,移植组左室舒张末压比对照组显著降低[(5.1±3.07)mmHgVS(11.67±3.42)mmHg,P〈0.01,1mmHg=0.1333kPa],移植组心输出量比对照组显著增加[(3.1±0.89)L/minvs(2.39±0.43)L/rain,P〈0.05];BM—MNCs移植后6周,移植组有明显的血管新生,移植组梗死边缘区血管数量明显高于对照组[(19.32±2.47)vs(9.47±1.28),P〈0.01],梗死区血管数量移植组与对照组无显著差异[(3.44±0.51)VS(3.07±03),P〉0.05]。【结论】经冠脉移植自体骨髓单个核细胞后,可改善急性心肌梗死后心功能,促进梗死边缘区血管生成。  相似文献   

10.
目的:建立运动诱发肺动脉高压(exercise induced PH,EiPH)大鼠模型,早期明确诊断,及时给予治疗,评估大鼠PH进展、肺血管重塑情况及生存率。方法:雄性Sprague Dawely(SD)大鼠60只,体质量200~220 g,大鼠注射野百合碱(monocrotaline,MCT)(60 mg/kg)后,分别在第1、3、5、7、9天检测大鼠静息状态下平均肺动脉压力(mean pulmonary artery pressure,mPAP)及运动状态下峰值mPAP(maximum mPAP,mPAPmax)。选取大鼠静息mPAP<25 mmHg且运动状态下mPAPmax>30 mmHg定义为EiPH组;将大鼠静息mPAP≥25 mmHg随机分为静息PH(rest PH,rPH)组和PH组;注射生理盐水的大鼠为对照组。EiPH组与rPH组大鼠分别给予等量他达那非灌胃2周。各组大鼠于实验终点完成超声心动图检查,改良右心导管测压,留取心肺组织。结果:MCT注射后第7天,大鼠运动状态下mPAPmax较对照组明显升高[(38.5±2.7) mmHg vs. (27.24±0.46) mmHg,P=0.000]。MCT注射后第35天,rPH+T组大鼠静息mPAP升高[(26.67±0.11) mmHg vs. (35.23±0.47) mmHg,P=0.000]、右室肥厚指数增加(0.34±0.01 vs. 0.46±0.02,P=0.004)、肺动脉中膜厚度百分比增加(44.4% vs. 55.6%,P=0.008)。结论:本研究成功建立EiPH大鼠模型。他达那非干预实验结果表明,早期阶段给予PH大鼠治疗的降压及抑制肺血管重构效果优于同等剂量药物的晚期用药。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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