齐墩果酸对雄性小鼠化学性肝损伤的作用研究

目的：探讨齐墩果酸对雄性小鼠化学性肝损伤的抑制作用。方法30只健康雄性小鼠按体重随机分成3组,分别为对照组、模型组和同时给药组。同时给药组注射四氯化碳( CCl4)的同时给予齐墩果酸灌胃,模型组注射CCl4的同时给予豆油灌胃,对照组注射生理盐水的同时给予豆油灌胃。检测脏器系数、血液和肝组织匀浆中超氧化物歧化酶( SOD)和丙二醛( MDA)的含量。结果 CCl4腹腔注射以后主要引起肝脏的化学性损伤,齐墩果酸未能有效抑制CCl4引起的血液和肝组织匀浆中SOD及MDA含量的改变。结论腹腔注射CCl4的同时给予齐墩果酸灌胃,不能有效抑制CCl4引起的雄性小鼠肝损伤。     

齐墩果酸对小鼠急性肝脏氧化损伤治疗作用研究

目的建立肝脏急性氧化损伤模型,观察齐墩果酸对小鼠肝脏氧化损伤的治疗作用。方法 3 m L/kg四氯化碳(CCl4)腹腔注射,24 h后给予齐墩果酸灌胃,干预2 d后处死小鼠。检测肝脏脏器系数、病理改变、超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果齐墩果酸未拮抗CCl4引起的SOD含量降低以及MDA含量升高(P>0.05),也未能减轻小鼠肝组织的损伤程度。结论 CCl4诱导小鼠急性肝脏氧化损伤以后,再给予齐墩果酸治疗,效果不佳。     

加味血府逐瘀汤对D-Ga1N所致肝损伤小鼠超氧化物歧化酶和丙二醛的影响

目的：观察加味血府逐瘀汤对 D-氨基半乳糖氨(D-GalN)所致肝损伤小鼠超氧化物歧化酶(SOD)和 丙二醛(MDA)的影响。方法：NIH 小鼠60只，随机分5组，分别为正常对照组，模型组，加味血府逐瘀汤高剂量组、 低剂量组和齐墩果酸片治疗组；加味血府逐瘀汤高、低剂量组和齐墩果酸片组小鼠分别灌胃给药，每天1次，连续4 天，末次给药1h 后，除正常对照组外，其余各组小鼠均腹腔注射 D-GalN800mg/kg，24h 后采血，检测血清 SOD 活 性、MDA 含量和谷丙转氨酶(ALT)活性，并观察肝组织病理学变化。结果：加味血府逐瘀汤高剂量组小鼠血清 SOD 的活性明显高于模型组(P＜0． 01) ，MDA 含量和 ALT 活性均明显低于模型组(P＜0． 01) ；与齐墩果酸片组比 较，加味血府逐瘀汤高剂量组 SOD 活性、MDA 含量和 ALT 活性差异均无显著性意义(P＞0． 05) ；加味血府逐瘀汤 高剂量组可明显减轻肝组织病理改变，与模型组比较差异有显著性意义(P＜0． 05) 。结论：加味血府逐瘀汤可提高 D-GalN 所致肝损伤小鼠 SOD 活性和降低 MDA 含量，并可明显减轻肝组织病理改变。     

玉米黄质对化学诱导小鼠急性肝损伤的预防研究

目的:研究玉米黄质对四氯化碳(CCl4)所致小鼠急性化学性肝损伤的保护作用。方法:雄性昆明种小鼠60只,随机分为正常对照组、急性化学性肝损伤模型组、联苯双酯阳性对照组以及玉米黄质低、中、高剂量组,测定并比较各组小鼠肝脏系数,血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性与丙二醛(MDA)含量;测定肝组织中SOD、GSH-Px活性、MDA含量以及组织病理系数。结果:玉米黄质各剂量组均能升高急性化学性肝损伤小鼠血清与肝组织SOD、GSH-Px活性(P<0.01),降低血清ALT、AST活性(P<0.01)和降低血清与肝组织MDA含量(P<0.01),并能不同程度地改善肝脏病理损伤。结论:玉米黄质对CCl4所致急性化学性肝损伤具有预防性保护作用。     

加味血府逐瘀汤对D-Ga1N所致肝损伤小鼠超氧化物歧化酶和丙二醛的影响

目的：观察加味血府逐瘀汤对D-氮基半乳糖氨（D-Ga1N）所致肝损伤小鼠超氧化物歧化酶（SOD）和丙二醛（MDA）的影响.方法：NIH小鼠60只,随机分5组,分别为正常对照组,模型组,加味血府逐瘀汤高剂量组、低剂量组和齐墩果酸片治疗组;加味血府逐瘀汤高、低剂量组和齐墩果酸片组小鼠分别灌胃给药,每天1次,连续4天,末次给药1h后,除正常对照组外,其余各组小鼠均腹腔注射D-Ga1N800mg/kg,24h后采血,检测血清SOD活性、MDA含量和谷丙转氨酶（ALT）活性,并观察肝组织病理学变化.结果：加味血府逐瘀汤高剂量组小鼠血清SOD的活性明显高于模型组（P＜0.01）,MDA含量和ALT活性均明显低于模型组（P＜0.01）;与齐墩果酸片组比较,加味血府逐瘀汤高剂量组SOD活性、MDA含量和ALT活性差异均无显著性意义（P＞0.05）;加味血府逐瘀汤高剂量组可明显减轻肝组织病理改变,与模型组比较差异有显著性意义（P＜0.05）.结论：加味血府逐瘀汤可提高D-Ga1N所致肝损伤小鼠SOD活性和降低MDA含量,并可明显减轻肝组织病理改变.     

泡桐花总黄酮对小鼠肝损伤的预防保护作用

目的探讨泡桐花总黄酮对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法 50只小鼠随机分为对照组、CCl4模型组、水飞蓟宾对照组、CCl4+黄酮低剂量组和CCl_4+黄酮高剂量组,每组10只。对照组和CCl_4模型组小鼠给予质量分数1%羧甲基纤维素钠(CMC-Na)10 m L·kg-1混悬液灌胃,水飞蓟宾对照组小鼠给予100 mg·kg~(-1)水飞蓟宾CMC-Na混悬液灌胃,CCl_4+黄酮低剂量组和CCl_4+黄酮高剂量组小鼠分别给予泡桐花总黄酮CMC-Na混悬液(100、200 mg·kg-1)灌胃,连续7 d,末次灌胃给药2 h后,对照组小鼠腹腔注射色拉油0.10 m L·kg-1,其余4组小鼠腹腔注射0.012 mol·L-1CCl4溶液10 m L·kg-1(色拉油配制)造成小鼠急性肝损伤,16 h(禁食不禁水12 h)后摘眼球采血,分离血清,检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平及肝脏组织中丙二醛(MDA)和过氧化物歧化酶(SOD)水平。结果与对照组比较,CCl_4模型组小鼠ALT、AST、MDA水平显著升高,SOD水平降低(P<0.01);与CCl4模型组比较,水飞蓟宾对照组、CCl4+黄酮低剂量组和CCl_4+黄酮高剂量组小鼠ALT、AST、MDA水平显著降低,SOD水平升高(P<0.05);CCl4+黄酮低剂量组小鼠ALT、AST、MDA、SOD水平与CCl4+黄酮高剂量组比较差异无统计学意义(P>0.05),CCl_4+黄酮低、高剂量组与水飞蓟宾对照组比较,差异无统计学意义(P>0.05)。结论泡桐花总黄酮对CCl4造成的急性肝损伤有明显的保护作用。     

D-阿洛糖对急性化学性肝损伤小鼠的保护作用研究

目的观察D-阿洛糖（D-aHose）对四氯化碳（ccl4）致小鼠急性化学性肝损伤的保护作用。方法建立小鼠急性肝损伤模型,检测D-阿洛糖预处理组和模型组、对照组的脏器系数和生化指标结果D-阿洛糖能降低肝脏系数,对肾脏、脾脏系数无明显影响;D-阿洛糖能显著降低肝损伤小鼠血清ALT、AST水平,降低肝组织匀浆MDA含量,稳定肝组织SOD、GSH-Px、GSH水平,呈现剂量依赖性。结论D-阿洛糖对CCl4致小鼠急性肝损伤有保护作用。     

水药酢浆草对四氯化碳致小鼠肝损伤的保护性研究

目的 探讨水药酢浆草对四氯化碳CCl4诱导的小鼠肝损伤的保护作用。方法 将40只小鼠随机分为5组,正常组、CCl4模型组、酢浆草低、中、高剂量组,每组8只。正常组和CCl4模型组给予生理氯化钠溶液,酢浆草低、中、高剂量组给分别给100、300及600 mg/kg酢浆草灌胃,连续5 d。于末次给药2 h后,除正常组外,其余各组小鼠给予0.3% CCl4花生油溶液稀释、按0.02 ml/g的剂量皮下注射,在中毒后16 h取血和肝脏组织,分别检测各组小鼠肝功能相关的生化指标。结果 与CCl4模型组小鼠比较,不同剂量酢浆草治疗组小鼠肝脏指数、血清ALT、AST及TNF-α水平不同程度降低（P <0.05）;肝组织中CAT、GSH及SOD水平不同程度升高（P <0.05）,MDA水平不同程度降低（P <0.05）。结论 水药酢浆草对CCl4所致小鼠肝损伤具有明显的保护作用,其机制可能与酢浆草降低氧化酶活性和抗脂质过氧化作用有关。     

肉苁蓉多糖对CCl4所致小鼠急性肝损伤的保护作用

目的：研究肉苁蓉多糖对CCl4所致小鼠急性肝损伤的保护作用。方法：采用CCl4急性肝损伤模型,通过小鼠灌胃给予高、低剂量的肉苁蓉多糖（400 mg／kg、100 mg／kg ）,并以联苯双酯（150 mg／kg ）为阳性对照药,检测小鼠血清的丙氨酸氨基转移酶（ALT）、天冬氨酸转氨酶（AST）及肝脏中丙二醛（MDA）、超氧化物歧化酶（SOD）的含量。结果：肉苁蓉多糖高剂量组小鼠的血清ALT、AST与CCl4模型组相比显著降低（ P＜0．05）,低剂量组ALT、AST与CCl4模型组相比差异无统计学意义,各剂量组MDA含量与模型组相比显著降低（ P ＜0．05）、SOD的活性显著增高（ P ＜0．05）。结论：肉苁蓉多糖对小鼠急性肝损伤具有一定的保护作用。     

葡萄籽提取物对化学性肝损伤的抗氧化保护作用

目的研究葡萄籽提取物(GSE)对四氯化碳(CCl4)诱导肝损伤的抗氧化保护作用。方法建立CCl4诱导肝损伤的大鼠模型,测定正常对照组、CCl4诱导组(CCl4组)、GSE低剂量组(20mg/kg·d)、GSE中剂量组(40mg/kg·d)和GSE高剂量组(120mg/kg·d)共5个组肝脏中总抗氧化能力(TAOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平和血清SOD、TAOC水平,称量肝重和体重,比较各组大鼠肝体比值、甘油三酯(TG)的改变。结果各组大鼠肝脏中TAOC值和SOD值差异无统计学意义;CCl4组和GSE低、中剂量组的肝脏中GSH-Px值高于对照组和GSE高剂量组;CCl4组肝脏MDA值组显著高于正常对照组及GSE低、中、高3个剂量组。GSE高剂量组的血清TAOC值显著高于其余4个组;高剂量组、中剂量组血清中SOD显著高于其余3组。5个组的肝体比值无统计学意义;CCl4组的肝脏中TG值高于正常对照组和GSE中剂量组。结论葡萄籽提取物可降低大鼠肝脏中TG和MDA含量,提高机体总抗氧化力,明显改善肝脏病理改变。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…