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1.
目的:观察辛伐他汀对急性心肌梗死后大鼠心室重塑及心功能的影响,并探讨其可能的机制.方法:结扎大鼠冠状动脉前降支造成心肌梗死模型,给予辛伐他汀干预.8周后,测定心脏重量指数、血流动力学指标、心肌及血液中总-氧化氮合酶(NOS)、诱导型一氧化氮合酶(iNOS)活性,并与假手术组比较.结果:心肌梗死组大鼠右室重量指数(RVWI)、左室重量指数(LVWI)升高(P<0.01),外周动脉压(APS)、舒张压(APD)均降低,心率增快(P<0.01).左室收缩压(LVSP)和心室收缩期室内压上升速率( dp/dtmax),心室舒张期室内压下降速率(-dp/dtmax)下降(P<0.01),左室舒张末压(LVEDP)升高(P<0.01).血清和心肌NOS、iNOS活性下降(P<0.01).辛伐他汀干预组较心肌梗死组(M组)RVWI、LVWI下降(P<0.01),APS、APD、LVSP、 dp/dtmax、-dp/dtmax均回升(P<0.01),LVEDP回落(P<0.01);血清和心肌NOS、iNOS活性升高.结论:辛伐他汀减轻左室重塑,改善心功能.  相似文献   

2.
袁侨英  覃数 《重庆医学》2004,33(11):1664-1665
目的观察辛伐他汀抑制急性心肌梗死后心肌氧化损伤的作用,并探讨其可能的机制.方法结扎大鼠冠状动脉前降支造成心肌梗死模型,给予辛伐他汀干预. 8周后,测定血流动力学指标、心肌及血液中总超氧化物歧化酶(SOD)、铜锌-超氧化物歧化酶(CuZn-SOD)活性;血液过氧化氢(H2O2)含量,并与假手术组比较. 结果心肌梗死组(M组)大鼠外周动脉压(APS)、舒张压(APD)均降低,心率增快(P<0.01).LVSP和 dp/dtmax,-dp/dtmax下降(P<0.01), LVEDP升高(P<0.01);心肌、血液中SOD及CuZn-SOD活性下降,H2O2含量升高(P<0.01).辛伐他汀干预组较M组APS、APD、LVSP、 dp/dtmax、-dp/dtmax均回升(P<0.01),LVEDP回落(P<0.01);血清和心肌SOD及CuZn-SOD活性升高,H2O2含量下降.结论辛伐他汀改善急性心肌梗死后心功能,可能与其提高SOD活性抗氧化损伤有关.  相似文献   

3.
目的 观察肝细胞生长因子(HGF)抑制急性心肌梗死后心肌氧化损伤的作用,并探讨其可能的机制.方法 结扎犬冠状动脉前降支制造心肌梗死模型,给予心肌内注射HGF裸质粒干预.4周后,测定HGF在心肌的表达,测量心脏质量指数、心肌及血液中总超氧化物歧化酶(SOD)、铜锌-超氧化物歧化酶(CuZn-SOD)活性,血液过氧化氢(H2O2)含量,并与假手术组比较.结果 心肌梗死组(M组)大鼠右室心脏质量指数(RVWI)、左室心脏质量指数(LVWI)升高(P<0.01),心肌、血液中SOD及CuZn-SOD活性下降,H2O2含量升高(P<0.01).HGF组较M组RVWI、LVWI下降(P<0.01),血清和心肌SOD及CuZn-SOD活性升高,H2O2含量下降.结论 HGF改善急性心肌梗死后心室重塑和心功能,可能与其提高SOD活性抗氧化损伤有关.  相似文献   

4.
张进  佘强 《重庆医科大学学报》2008,33(11):1349-1352
目的:研究氯沙坦(Losanan)干预对急性心梗(Acute myocardial infarction,AMI)大鼠左室非梗死区(Left ventficular non-infaretion zone,LVNIZ)蛋白激酶C(Protein kinase C,PKC)、ERK1/ERK2、p-ERK1/ERK2及心室重塑(Left ventricular remodeling,LVRM)的影响.方法:36只雄性Wistar心肌梗死大鼠随机分为:AMI组(n=30)(按照术后1、3、7、14、28 d5个时间点分为5组)及Losartan组(n=6)(灌胃给药,20 mg/(kg·d),持续4周);另设Sham 7 d组(n=6)作为空白对照.多导生物信号分析系统检测左室舒张末压(Left ventricalar end-1diastolic pressure,LVEDP);心脏取出后测定左室重量指数(Left ventricular weight index,LVWI);放射免疫法测定血浆及非梗死区心肌组织血管紧张素Ⅱ(AngⅡ)水平;Western-blot检测PKC、ERK1/ERK2、p-ERK1/ERK2的表达.结果:AMI-28d组LVEDP、LVWI及LVNIZ AngⅡ水平显著升高(P<0.0与Sham相比较);大鼠心肌梗死24 hA非梗死区PKC、ERKl/ERK2、p-ERK1/ERK2的表达均增强,4周后达到高峰(P<0.01与Sham相比较);直线相关分析显示,AMI-28d后非梗死区PKC与AngⅡ正相关(P<0.05).p-ERK1与PKC、LVEDP、LVWI呈显著正相关(P<0.01).与AMI-28 d组相比,Losartan组LVRM明显减轻,PKC、ERK1/ERK2、p-ERK1/ERK2的表达显著降低(P<0.01).结论:心肌梗死后心脏局部组织AngⅡ可激活PKC-ERK1/ERK2信号通路,Losartan改善LVRM的机制可能为Losartan阻断了AngⅡ的有害作用,进而阻断了PKC-ERK1/ERK2信号通路介导的心室重塑.  相似文献   

5.
目的:探讨苯那普利、厄贝沙坦及两者联合用药对心衰大鼠心脏指数及血流动力学的影响。方法:采用缩窄大鼠腹主动脉造成压力负荷性心肌肥厚致心力衰竭的大鼠模型。随机分为5组:假手术组、模型组、苯那普利组、厄贝沙坦组及联合用药组,连续给药8周,检测心脏指数(HMI、LVMI)及血流动力学参数:LVSP,LVEDP,dp/dtmax,-dp/dtmax;放免法检测血浆和心肌AngⅡ的含量。结果:模型组HMI、LVMI均显著升高(P<0.01),各治疗组显著下降(P<0.01),联合组较单用明显(P<0.05);与模型组相比,苯那普利组及联合用药组AngⅡ的含量显著降低(P<0.01),而厄贝沙坦组AngⅡ含量增高;与假手术组相比,模型组LVEDP显著升高(P<0.01),各治疗组均有不同程度的下降(P<0.01),且联合用药组较单用组明显(P<0.05);模型组LVSP、dp/dtmax明显下降(P<0.01),治疗后,各组有不同程度上升(P<0.01)。结论:联合应用苯那普利和厄贝沙坦对改善心衰大鼠心脏指数及血流动力学具有协同作用。  相似文献   

6.
辛伐他汀抑制大鼠急性心肌梗死后炎症因子的实验研究   总被引:1,自引:0,他引:1  
目的:在大鼠结扎前降支致心肌梗死的模型上观察辛伐他汀抑制心肌梗死后炎症因子的作用,探讨辛伐他汀改善心室重塑和心功能的机制。方法:结扎大鼠冠状动脉前降支造成心肌梗死模型,给予辛伐他汀干预;8周后,测定心脏重量指数、心肌梗死区与非梗死区心肌羟脯氨酸含量和胶原含量、检测不同分组大鼠心肌组织中TNF-α、IL-6和IL-10水平差异,并与假手术组比较。结果:心肌梗死组(M组)大鼠右室重量指数[RVWI右室心脏重量指数=右室心脏重量(mg)/体重(g)]为0.803±0.010、左室重量指数[LVWI左室心脏重量指数=左室心脏重(mg)量/体重(g)]为3.26±0.05,较假手术组(S组)升高(p<0.01)。心肌梗死组(M组)心肌组织中羟脯氨酸、胶原含量(mg/g)、TNF-α和IL-6(pg/ml)表达升高(分别为5.528±0.59、40.58±4.31、20.83±5.79、109.88±22.68)(p<0.01),IL-10(pg/ml)表达降低(25.17±5.52)(p<0.05)。辛伐他汀干预组较M组RVWI、LVWI(mg/g)下降(0.690±0.011、2.42±0.04)(p<0.01),心肌中羟脯氨酸、胶原含量(mg/g)及TNF-α、IL-6(pg/ml)表达下降(分别为3.40±0.78、24.95±5.74、21.15±6.01、156.48±32.82)(p<0.01),IL-10(pg/ml)表达升高(24.88±5.44)(p<0.01)。结论:辛伐他汀可减少急性心肌梗死后有害炎症因子表达,并抑制胶原形成及心肌纤维化,可能与其改善心室重塑和心功能有明显关系。  相似文献   

7.
廖蓉 《重庆医学》2014,(27):3596-3598
目的:建立大鼠心肌梗死的模型,观察不同剂量辛伐他汀抑制心肌梗死后氧化应激的作用,探讨不同剂量辛伐他汀改善大鼠心室重塑和心功能的潜在机制。方法结扎大鼠冠状动脉前降支,形成急性心肌梗死模型,给予不同剂量辛伐他汀(20、40、60 mg · kg -1· d-1)干预。4周后,测定心脏心室重塑的各项指标,检测不同分组的大鼠心肌中总超氧化物歧化酶(SOD )、铜锌-超氧化物歧化酶(CuZn-SOD)活性、血液H2 O2水平,并与假手术组比较。结果心肌梗死组大鼠心室重塑明显,左室心脏重量指数(LVWI)升高,心率增快,血流动力学紊乱(P<0.05)。心肌中SOD、CuZn-SOD和血液中 H2O2水平升高(P<0.05)。不同剂量辛伐他汀干预组均较心肌梗死组LVWI下降(P<0.05),心肌中SOD、CuZn-SOD和血液中 H2 O2水平下降,心率减慢,血流动力学改善(P<0.05),尤其以高剂量他汀干预组(60 mg · kg -1· d-1)更明显。结论辛伐他汀减少急性心肌梗死后氧化应激表达,可能是改善心室重塑和心功能的机制之一,并与剂量有一定的正相关性。  相似文献   

8.
目的:评价鹿红颗粒对心肌梗死后心力衰竭(CHF)模型大鼠血流动力学及血浆血管紧张素Ⅱ(AngⅡ)和醛固酮(ALD)的影响。方法:60只SD大鼠随机分配至模型组、假手术组、雅施达组及鹿红颗粒高、中、低剂量组,结扎左冠状动脉的方法制备心肌梗死后心力衰竭模型。药物干预8周后用电生理仪记录血流动力学变化,包括左室收缩压(LVSP)、左室舒张末压(LVEDP)、左室内压最大上升和下降速率(±dp/dtmax);放射免疫分析法检测AngⅡ及ALD。结果:雅施达、鹿红颗粒高、中剂量均能有效升高LVSP、降低LVEDP、升高+d P/dtmax及-d P/dtmax(P0.01),且鹿红颗粒高、中剂量组在改善血流动力学指标上优于雅施达组(P0.01),而高、中剂量组之间无显著差异(P0.05);雅施达与鹿红颗粒高、中剂量均能有效降低模型大鼠血浆AngⅡ和ALD的水平(P0.01),而鹿红颗粒高、中剂量略差于雅施达(P0.01),高、中剂量组之间无显著差异(P0.05)。结论:鹿红颗粒能显著改善心力衰竭大鼠心脏血流动力学参数,降低心力衰竭大鼠血浆AngⅡ和ALD水平,从而改善心功能,延缓CHF病程进展。  相似文献   

9.
葛根素对压力超负荷兔心力衰竭及神经内分泌激素的影响   总被引:4,自引:0,他引:4  
目的 :探讨葛根素对压力超负荷兔心力衰竭及其对心钠素 (ANP)和血管紧张素Ⅱ (AngⅡ )的影响。 方法 :将兔随机分为假手术组、手术对照组和葛根素组 ,采用腹主动脉次全结扎 ,建造兔心力衰竭模型 ;经颈动脉插管 ,利用BL -4 2 0E生物机能实验系统记录平均动脉压 (MAP)、心率 (HR)、左室收缩峰压 (LVSP)、左室舒张末压 (LVEDP)、最大上升速率 ( +dp/dtmax)和最大下降速率 ( -dp/dtmax)等血流动力学参数 ;放免法测定血浆ANP和AngⅡ水平。 结果 :( 1)手术对照组与假手术组相比 ,血流动力学参数明显异常 (P <0 .0 0 1) ;( 2 )葛根素组与手术对照组相比 ,HR减慢 ,±dp/dtmax明显上升 ,LVEDP明显下降 (P <0 .0 0 1) ;( 3 )葛根素组与手术对照组相比 ,血浆ANP浓度明显下降(P <0 .0 0 1) ,AngⅡ水平亦显著降低 (P <0 .0 5 )。 结论 :葛根素能减缓压力超负荷兔心力衰竭的发生 ,其机理可能是减少了ANP和AngⅡ的释放  相似文献   

10.
《右江医学》2017,(1):15-19
目的探讨龙血竭对急性心肌梗死家兔左心功能的保护作用。方法将30只家兔随机分为三组,假手术组、心肌梗死组、龙血竭干预组,每组10只。采用左冠状动脉前降支结扎法,建立急性心肌梗死模型。观察HE染色的心肌组织结构变化,测量心率、左心室收缩末压(LVSP)、左心室舒张末压(LVEDP)、左心室内压最大上升速率(+dp/dtmax)、左心室内压最大下降速率(-dp/dtmax)。结果龙血竭干预组心肌组织结构明显好于心肌梗死组;心肌梗死组和龙血竭干预组其心率较假手术组明显减慢,龙血竭干预组心率减慢更明显(P<0.05或0.01)。心肌梗死组、龙血竭干预组与假手术组比较,其LVSP均明显下降、LVEDP均明显升高,而心肌梗死组与龙血竭干预组比较,其LVSP下降更明显、LVEDP升高更明显(P<0.05或0.01)。心肌梗死组和龙血竭干预组其+dp/dtmax和-dp/dtmax均较假手术组明显下降,且心肌梗死组较龙血竭干预组下降更明显,差异有统计学意义(P<0.01)。结论龙血竭对急性心肌梗死家兔左心功能具有较好的保护作用。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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