砖茶型氟铝联合应用对大鼠牙齿和骨代谢生化指标的影响

目的 观察砖茶型氟铝联合中毒对大鼠牙齿和骨代谢相关血清学指标的影响,以定量评估氟和铝对牙齿和骨代谢的损伤作用。方法 5周龄SPF级Wistar大鼠70只,随机分为7组,正常对照组,氟组,铝组,氟铝组,高剂量高氟高铝砖茶组,低剂量高氟高铝砖茶组,低氟低铝砖茶组。定期观察大鼠牙齿状态,使用酶联免疫吸附实验检测大鼠血清PTH、OT、CT水平,并且进行血清Ca、P、ALP的检测。结果 氟组、氟铝组、高剂量高氟高铝砖茶组均出现不同程度的氟斑牙;铝组除个别出现牙釉质光泽度消失外未见氟斑牙表现;低氟低铝砖茶组仅出现一例轻度氟斑牙。氟组、氟铝组、高剂量高氟高铝砖茶组的血清PTH、OT都高于对照组(P<0.01),且氟组最高;而铝组PTH,OT,ALP均较对照组降低(P<0.01);氟铝组和所有砖茶组的血清Ca水平都低于对照组(P<0.01),且低剂量高氟高铝砖茶组最低;氟组,氟铝组和高剂量高氟高铝砖茶组血清CT均比对照组低(P<0.01),以氟铝组最低,但铝组则呈显著性升高(P<0.01)。结论 本次研究的剂量条件下单氟对牙齿的损伤作用较明显,氟铝联合作用时表现为拮抗作用,且OT、PTH、CT和ALP能间接的反应骨骼的损伤情况。     

砖茶型氟铝联合中毒大鼠脑氧化应激水平变化

目的 通过对长期大量饮用砖茶大鼠脑组织丙二醛、谷胱甘肽过氧化物酶、谷胱甘肽S转移酶和总超氧化物歧化酶含量的检测，探讨砖茶型氟铝联合中毒发生的病理生理学机制，并为长期饮用砖茶地区氟铝联合中毒的预防提供参考。方法 5周龄SPF级Wistar大鼠70只，雌雄各半，随机分为7组，分别为正常对照组、氟组、铝组、氟铝组、高剂量高氟高铝砖茶组、低剂量高氟高铝砖茶组、低氟低铝砖茶组。12个月时处死大鼠，通过检测大鼠脑组织MDA含量及T-SOD、GSH-PX、GST的活力，来评价其氧化应激水平。结果 各化学剂量组大鼠脑组织T-SOD、GSH-PX、GST均显著低于对照组（P<0.01），氟组和铝组MDA均显著高与对照组（P<0.01），高剂量高氟高铝砖茶组GSH-PX（P<0.01），GST（P<0.01）均显著高于对照组，低氟低铝砖茶组T-SOD（P<0.01）、GSH-PX（P<0.01）均显著高于对照组。结论 氟和铝之间可能存在拮抗作用，低氟低铝砖茶组具有很好的抗氧化作用，可提倡饮用低氟低铝砖茶。     

大鼠摄入铝、氟及其不同比例混合物后血和骨中铝氟含量分析

将大鼠分为对照、单纯氟、单纯高、中、低剂量铝及高、中、低剂量铝加氟混合等8个组,分别以拌饲喂养12周,取全血或血清及肱骨,作氟、铝、钙、磷定量分析。结果显示:单纯氟组的血和骨氟均升高,以骨氟为甚,超过对照组十多倍;单铝组的血和骨铝与投量不平行,中铝组反比高铝组高;铝氟混合各组的血和骨氟均下降,血氟可恢复至正常水平,但骨氟则否,显示铝拮抗氟的吸收。若以血和骨铝为指标,则低、中剂量铝是氟拮抗铝的吸收,高剂量铝则氟增强铝的吸收;实验中血清磷均升高,血清钙不受干扰,骨钙、骨磷则在单纯氟组、高铝组及高铝加氟组均下降。作者对铝吸收与投铝量不平行及氟对吸收的双相影响的机理进行了讨论。     

砖茶型氟铝联合中毒的实验研究

目的:复制砖茶型氟铝联合中毒动物模型。方法:通过检测动物骨、肝、肾、脑组织和血清中的氟、铝含量观察氟和铝在体内代谢的情况。结果:至实验期末各实验组动物体重均显著低于对照组(P<0.05),其中砖茶组动物体重降低更加显著(P<0.01);砖茶组动物各组织中氟、铝含量与对照组比较极显著增高(P<0.01)。结论:长期大量饮用砖茶水可导致砖茶型氟铝联合中毒;高氟可促进铝吸收并蓄积在骨组织中。     

氟铝联合对体外培养人胚成骨细胞生长发育的影响

目的：探讨氟铝联合对体外培养人胚成骨细胞生长发育的影响。方法：用无血清体外细胞培养方法，将成骨细胞分成空白对照组、低铝组、高铝组、低铝低氟组、高铝低氟组、高铝高氟组共6组，观察氟铝共存对其生长发育的影响。结果：加入氟铝后第14天和第16天，高铝组及高铝低氟组的细胞存活数均显著低于对照组、低铝组和低铝低氟组（P＜0．05）。高铝组的细胞变化坏死率明显高于对照组、低铝组及低铝低氟组（P＜0．05），高铝组加入不同剂量氟可抑制细胞的变性坏死率，但仍明显高于对照组、低铝组和低铝低氟组（P＜0．05），而低于高铝组（P＜0．05）。结论：高剂量铝可抑制体外培养的人胚成骨细胞的生长发育并对其产生一定的毒作用，在高铝情况下，加入一定量氟可抑制铝对成骨细胞的毒作用。     

不同比例铝氟混合物联合作用的急性实验研究

作者用9种不同比例的铝与氟混合物对雄性Wistar大鼠急性染毒,以常用的4种检验联合作用的方法,评价其联合作用的性质。结果显示,铝与氟之间的联合作用主要表现为铝对氟的拮抗作用。拮抗作用范围Al:F为0.8:0.2～0.1:0.9之间,最适拮抗作用Al:F为0.3:0.7～0.1:0.9之间,铝含量低于或等于5％,高于或等于90％时,无拮抗作用。     

氟铝联合中毒研究进展

氟和铝广泛存在于自然界中,都是人体所必需的微量元素之一。以往的研究发现,氟过量或铝过量均会引起不同程度的氟中毒或是铝中毒。在氟铝之间又存在相互作用,氟铝联合中毒是1984年由贵州省六盘水市卫生防疫站发现的一种新的中毒形式。它解释了许多关于"单纯"氟中毒和"单纯"铝中毒的不足之处,同时也为更好的临床诊治提供了理论依据。为进一步做好氟铝联合中毒的研究工作,本文就近20年来国内外众多学者做的氟铝联合中毒予以综述。     

氟铝联合对体外人胚大脑神经细胞生长发育的影响

目的：探讨氟铝联合对体外人胚大脑神经细胞生长发育的影响。方法：采用体外人胚大脑神经细胞,分为６ 组观察氟铝共存对其生长发育的影响。结果：大脑神经细胞的存活数高剂量铝组低于低剂量铝组和对照组,有显著性差别（Ｐ＜ ０．０５）。结论：高剂量铝组可抑制人胚大脑神经细胞的生长发育,加入不同剂量氟并未对铝的作用产生明显的拮抗或协同作用     

氟、砷及氟砷联合作用对小鼠肝、脑、肾氧化损伤的实验研究

目的　探讨氟、砷及氟砷联合作用对肝、脑、肾损害的机理。方法　用高氟、高砷、高氟高砷饲料分别饲养小鼠 12mo ,分别检测肝、脑、肾的LPO和GSH含量和SOD及GSH Px活性。结果　与对照组比较 ,氟组、砷组及氟砷联合组GSH含量均显著降低 ;各组SOD活力显著降低 ;除脑组织GSH PX降低不明显外 ,其余各组均显著降低。结论　氟、砷及氟砷联合作用均可使小鼠肝、脑、肾LPO增强 ,抗氧化能力降低 ,而且发现氟砷联合拮抗作用并不明显。     

地方性氟中毒病区高铝氟负荷骨变形儿童血清Ca^2＋、P^5＋、Mg^2＋、Fe^2＋、Zn^2＋含量检测分析

目的探讨地方性氟中毒病区内高铝氟负荷骨变形儿童的血清生化变化特征。方法对水城县纸厂乡氟病村内骨变形儿童18例及同龄对照,采空腹静脉血分离血清,用东芝120全自动生化检测分析仪及其配套试剂,同批检测白蛋白,离子态钙（ca”）、磷（P^5＋）、镁（Mg^2＋）、铁（Fe^3＋）、锌（zn^2＋）及白蛋白含量。结果骨变形儿童头发、尿铝增高2～3倍,尿氟增高246倍,血清ca^2＋（2.25±0.14）、Mg^2＋ G0.86范围o6～13mmol／L,Fe^3＋（13.65±3．84）、zn^2＋（9．95±4.58）umol／L,分别是非病区对照组的92．2％、72.3％、68.4％、66.5％,差异有统计学意义（P〈0.05～〈0.01）;磷离子（1.50±0.22）mmol／L是非病区对照组的104％,血清白蛋白（4714±240g／L为101％,差异无统计学意义,但P^5＋超过正常参考值上限。结论本组高铝氟负荷儿童的血清ca^2＋、Mg^2＋、Fe^2＋、zn^2＋水平低下和血清P^5＋异常,是铝氟联合作用使机体形成的非营养不良性钙镁铁锌生物有效利用不良,此与铝氟病的相同是新发现的地方性铝氟病患者。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…