Clinical evaluation of Deep Anterior Lamellar Keratoplasty (DALK) for stromal corneal opacities

Background

Corneal scars are commonly formed following many diseases of the eye like trauma, inflammation and infections. They lead to permanent diminution of vision which can be managed by Penetrating Keratoplasty (PK). PK is removing diseased as well as healthy tissues and is associated with many post-operative complications. Deep Anterior Lamellar Keratoplasty (DALK) is a relatively newer procedure which replaces only the diseased stroma, leaving the original corneal endothelium intact. This procedure is associated with lesser incidence of post-operative complications.

Methods

The study was conducted at a large tertiary care centre. 10 patients with stromal corneal scars were subjected to DALK and results were analysed after 06 months. Poor quality donor corneal tissue of B− and C grade was used in all cases.

Results

7 out of 10 patients (70%) undergoing DALK had post-operative visual acuity of 6/24 or better. 03 patients who did not have adequate recovery of visual acuity were due to over-riding of the graft in 01 case (10%), fungal keratitis in 01 case (10%) and interface haze in 01 case (10%).

Conclusion

DALK is a promising new technique for management of superficial corneal stromal scars using poor quality donor corneal tissue. Initial results are encouraging with minimal complications.     

AC/PC Intraocular Lens Exchange with Penetrating Keratoplasty in Pseudophakic Bullous Keratopathy

Background

Evaluation of penetrating keratoplasty in cases of pseudophakic bullous keratopathy with AC/PC IOL exchange.

Methods

This retrospective study included 120 cases of pseudophakic bullous keratopathy managed over 9 years at three tertiary care eye centres followed up for 4 years. Cases were taken up for penetrating keratoplasty along within adjuvant procedures like IOL explantation and Secondary Posterior Chamber IOL implantation over the frill of posterior capsule.

Results

Lens exchange with Penetrating Keratoplasty (PK) was done in 93 and PK without lens exchange in 27 cases. 25% required systemic steroids for 2-3 weeks. Re-grafting was performed in 5% and 85% attained moderate visual acuity.

Conclusion

Intra ocular lens exchange and Posterior chamber IOL are suitable for penetrating keratoplasty in terms of optical clarity, graft survival and visual outcome.Key Words: PBK, IOL exchange (AC/PC)     

Current techniques of lamellar keratoplasty for keratoconus

In recent years, there has been a considerable interest regarding the concept of lamellar keratoplasty (LK), which contributed in spreading the use of this procedure in the treatment of keratoconus. This is a new frontier in corneal surgery that minimizes trauma on the recipient patient since it works on a “closed bulb”. The LK surgery, in fact, aims to selectively replace diseased corneal stroma, leaving the healthy endothelium. The main advantage of LK is to avoid major causes of failure of penetrating keratoplasty as immunological rejection, and the late mismatch in the transplanted cornea, thus increasing the life of transplantation. In the last decade, several techniques of LK have been proposed, depending on how the anterior portion of the recipient cornea is removed. This article, through a literary research reviews the various emerging techniques of anterior lamellar surgery for the management of keratoconus, analyzing their indications, visual outcomes, and rate of complications.Keratoplasty has developed rapidly in the past 10 years and penetrating keratoplasty (PK), a procedure consisting of full-thickness replacement of the cornea, has been the dominant procedure for more than half a century.1-3 However, in recent years, cases in which corneal disease does not involve the endothelium, lamellar technique has rapidly replaced penetrating grafts with better clinical results.4-5 This technique aims to selectively replace diseased corneal stroma in a way to minimize unnecessary replacement of the unaffected healthy endothelial layer. Thus, by retaining the patient’s own endothelium, the risk of endothelial rejection, a major cause of graft failure in PK is almost eliminated, and endothelial cell density is preserved.4-6 Consequently, there is no need for long-term immunosuppressive therapy with corticosteroids, decreasing the risk of cataract, glaucoma, and infections. Fasolo et al7 in their corneal transplant epidemiologic study have reported the first results on corneal graft survival in Italy. They estimate the graft survival to be 95% for PK, and 93% for anterior lamellar keratoplasty (LK) after one year, showing a decrease in the survival rate along the considered period. Indeed, in the LK group, they observed a stable 3-year survival rate of 93%, whereas patients who underwent PK showed a decrease of the graft survival rate after one year. From the results of a recent meta-analysis conducted by Liu et al8 on the efficacy and safety of LK versus PK, it appears that in terms of spherical equivalent, central corneal thickness, and astigmatism there are no significant differences between the 2 procedures. Nevertheless, fewer complications occur in the LK group, in which the corneal endothelial density is higher than in the PK group.8 An additional advantage of LK compared with PK is that sutures can be removed earlier, and visual recovery occurs sooner. Furthermore, since LK is an extra-ocular procedure, it lacks the risk associated with open eye surgery, such as expulsive hemorrhage, endophthalmitis, and iris/lens damage.9 The purpose of this article is to analyze the current techniques of LK surgery in the treatment of keratoconus, reviewing updated available literature.

Lamellar keratoplastyamellar with augmented thickness

Recent years have brought on a sea of change in the field of corneal transplantation with PK being phased into newer anterior LK techniques. In keratoconus, the aim of surgery is to augment a thin and steep cornea, and this can be achieved using a thick donor lamellar of normal curvature, thus tectonically strengthening the cornea, which reduces irregular astigmatism and subsequent ectasia, and reducing corneal steepness, and concomitant high myopia.Thanks to the advent of new surgical devices, such as advanced microkeratome instrumentation, Excimer laser, and femtosecond laser, the results of lamellar techniques have been encouraging, with rapid visual rehabilitation and reduced risk of immune-mediated transplant rejection (Figure 1).10 With the increased availability of automated microkeratome for refractive procedures, such as Excimer laser in situ keratomileusis (LASIK), the use of microkeratome has increasingly been applied for dissection in LK. In the past, several authors11-13 have ventured in the LK surgery experiencing different techniques with the aid of microkeratome. Bilgihan et al14 described the “stromal sandwich technique”, consisting in the transplantation of a stromal button from a donor cornea under a corneal flap created in the host cornea. Busin et al15 described a more complex procedures suturing in the host bed a thicker and smaller lamella under tension, thus flattening the cone, restoring a normal corneal shape. Their surgery was not aimed at simply removing the central diseased corneal tissue and exchanging it with a healthy graft, but even at the remodeling of the ectatic cornea. Busin et al16 in 2012, trying to achieve a final corneal shape as similar as possible to the physiologic curvature of the donor cornea, introduced a modification of the microkeratome-assisted LK technique, including a full-thickness trephination of the residual bed before suturing the donor graft in place. They postulated that the recipient’s residual stroma can preserve a “keratoconus memory”, so through the disruption of the recipient’s architecture, they achieved a better postoperative refractive error, and spectacle-corrected visual acuity.Open in a separate windowFigure 1Forms of anterior lamellar keratoplasty (ALK) procedures available for keratoconus.To simplify and standardize LK, Excimer laser ablation has been used to prepare the recipient bed, with encouraging results. Excimer laser lamellar keratoplasty (ELLK) of augmented thickness is a procedure in which a deep plano excimer laser ablation is performed on the host cornea and a donor lamellar button, with or without an excimer laser refractive ablation on the posterior surface, is sutured into the recipient bed.17 According to Serdarevic et al18 the overriding advantage of using an ELLK is the laser’s ability to remove tissue with a microscopic precision that is unattainable with other procedures. They assert that the laser does not interfere with wound-healing processes, including cell migration and proliferation, and production of new tissue. In 1992, Kubota et al19 examined the depth of ablation of the recipient bed with different counts of oscillations of an excimer laser beam to determine the correlation between planned and real depth. Their results showed that an excimer laser achieved a precise cut in terms of diameter, site, and in particular thickness, indicating its utility in reproducible corneal photo ablation in LK. Buratto et al17 in their keratoconic eyes series, treated with laser LK of augmented thickness, reported better results compared with those treated with PK after 18 months. They found that this technique accelerated epithelialization, facilitated suturing the donor button to the recipient cornea, and produced a considerable flattening effect. Bilgihan et al20 obtained similar results after treating 5 keratoconus patients with ELLK. Excimer laser-assisted dissection was found to be a reproducible technique that requires short surgical time in a subsequent study of 2009, in which anatomical and functional results were evaluated for 41 patients with keratoconus after ELLK. The procedure consisted of a mechanical deepithelialization and a phototherapeutic keratectomy (PTK) using an excimer laser with a 7.0 mm round stainless steel mask placed on the cornea to create a vertical and regular edge of the ablation. The goal of a minimum estimated residual corneal bed was 200 µm. A 2.5 mm stromal pocket was created around the circumference of the ablation floor in order to receive the donor lamella, obtained by means of a microkeratome, and then secured in the recipient bed with 16 interrupted 10-0 nylon sutures. The uncorrected distance visual acuity (UDVA) and the corrected distance visual acuity (CDVA) were significantly better during all follow-up examinations than preoperatively, thus showing that ELLK is as efficacious as PK for the surgical treatment of moderate to advanced keratoconus.21 However, in some cases the mechanical effects of the recipient’s original keratoconus persisted, especially in eyes with advanced and decentered ectasia.20 The introduction of a new-generation excimer laser with a comprehensive surgical planning application specific for laser lamellar transplantations, allowed the surgeon to create custom ablations for both the receiving bed and the lamella. In this way it is possible to plan different ablation depths in the same cornea as a function of corneal thickness differentials. Studies demonstrated that the custom technique provided a satisfactory increase in corneal thickness, restoring structural and optical integrity to the tissue (Figure 2).22Open in a separate windowFigure 2Biomicroscopic examination one month after custom excimer laser-assisted lamellar keratoplasty in a 31-year-old keratoconus patient. Sixteen interrupted 10-0 nylon sutures and a clear cornea are visible.To reduce postoperative refractive errors in patients who had previously undergone LK for keratoconus, various techniques have been proposed, like excimer laser photorefractive keratectomy (PRK) and LASIK. However, a case of corneal ectasia was reported, following both excimer laser PRK and prior excimer laser-assisted LK for keratoconus, which was then successfully treated by corneal collagen crosslinking (CXL). This technique appeared to stabilize and partially reverse keratectasia in the 2-year postoperative follow-up period.23 Recently, the results of using combined treatment of customized PRK and prophylactic CXL in a group of patients with high ametropia and irregular astigmatism after ELLK have been reported. The mean correction of the spherical equivalent refractive error was greatly reduced, with an improvement in UDVA in all patients. Thus, demonstrating that the combination of these 2 treatments was safe and effective.24The femtosecond laser provides a novel approach for corneal transplantation due to its accuracy and predictability. It uses a type of near infrared light with a wavelength of 1053 nm, which yields the shortest pulse obtainable and can focus on quite a limited area, smaller than a hair’s breadth.25,26 Thanks to these features, femtosecond laser cutting yields a smooth stromal interface, increasing the safety and precision of corneal transplantation.27 Both the donor graft and the recipient corneal lenticule are created using femtosecond laser, the host lenticule smaller in diameter than the donor and sutured with 10/0 nylon.28 Compared with mechanical microkeratome, the femtosecond laser presents a cutting accuracy twice as high, with a standard deviation of depth of cutting to be 12-18 µm, against the 20-60 µm with the microkeratome.29-31 Femtosecond laser LK can be performed with or without sutures, allowing for early visual rehabilitation inducing less astigmatism and avoiding other suture-related complications.32-36 With the aim to provide a more stable grafted cornea it is possible to increase the touch area, facilitating the cicatrisation between the donor and recipient cornea by increasing the side cut angle.36 If the graft was not sutured to the recipient cornea a bandage soft contact lens for 3-12 days was used, with no case of graft dehiscence.37 However, Chan et al38 noted the variability in stromal thickness in eyes with advanced keratoconus may limit the ability of the femtosecond laser to produce a uniform lamellar plane while leaving a minimal amount of residual corneal tissue. As well, given the potential risk of creating a descemet membrane perforation, they found it more safe to perform a manual dissection of the posterior lamella.

Deep anterior lamellar keratoplasty (DALK)

The author who first introduced the concept of “deep anterior lamellar keratoplasty” as a dissection of host tissue close to the descemet’s membrane (DM) was Anwar in 1972.39 He noted that this procedure led to the formation of a smooth and transparent recipient bed, with a functional outcome similar to that of PK. He also explained the importance of removing the layer endothelium-descemet from the donor edge to avoid an inflammatory reaction and an irregular interface.Over the years, several surgical techniques have been studied and performed in order to obtain DM baring (Figure 1). When eliminating as much recipient tissue as possible, it is unclear if full stromal removal provides better results than cases, in which a small portion of the posterior stroma is left in place. However, despite the confused nomenclature, some surgeons refer to pre-descemetic DALK when LK enables the removal of 3 quarters or more of stroma to the deeper layers. This denotes that at least some posterior stromal layers are retained, and baring of DM is not achieved. According to Sarnicola et al,40 this could represent an advantage during the procedure of dissection of the host tissue, because he found that reaching a pre-descemetic level, the residual amount of stroma left in place prevented the microperforations from becoming macroperforations.

Pre-descemetic DALK

Current pre-descemetic DALK surgical techniques involve manual or microkeratome-assisted dissection near the DM. In 1997, Sugita and Kondo41 showed that there are no differences in visual acuity using a manual dissection technique that leaves a small amount of stroma, in place of the complete stromal dissection. Marchini et al42 intentionally left a minimal stromal thickness of 50 µm in order to reduce the risk of DM rupture. They used a vivo confocal microscopy to evaluate interface parameters (depth and reflectivity) of keratocyte and endothelial cell density over a 12-month follow-up, confirming Sugita and Kondo’s observation. Ardjomand et al43 compared visual function after DALK and after PK in 32 eyes with keratoconus and correlated it with corneal thickness. They demonstrated that eyes with a recipient corneal bed thickness of less than 20 µm had visual acuity similar to eyes with a PK, whereas those with a recipient thickness of greater than 80 µm had a significantly reduced visual acuity. Rama et al44 used a manual dissection technique guided by a calibrated knife incision based on ultrasonic pachymetry values. They treated 288 eyes and, according to Ardjomand et al,43 showed that eyes with lower values of recipient residue thickness are associated with better visual acuity. This could be explained by the fact that the residual bed thickness can determine the stiffness and corresponding resistance against the compressive forces of the donor graft. So it’s been hypothesized that thick residual recipient bed, together with a steep preoperative cornea and greater axial length, contribute to postoperative myopia in DALK.45 These results are consistent with several clinical studies46-48 available in literature comparing the visual outcome of descemetic and pre-descemetic DALK. However, pre-descemetic dissection is also indicated in cases of previous hydrops due to the risk that the DM will rupture at the hydrops scar. Chew et al49 describe a case of a boy with keratoconus and resolved hydrops who underwent bilateral manual DALK without baring of the DM. It demonstrated that good spectacle-corrected visual acuity can be achieved despite leaving a thin residual layer of the stroma unexcised. Consistent with this result, Anwar50 demonstrates the efficacy of a planned near-descemet dissection of the DALK in 22 patients with post-hydrops corneal scarring irregularity or corneal thinning because any surface irregularities are likely to be translated onto the final lamellar bed.50-51

Descemetic DALK

In 1972 Anwar39 first introduced his layer-by-layer manual dissection technique and despite have gone more than 40 years, it is still performed in some cases, such as pre-existing corneal perforation, strong stroma to DM adhesion, or inadequate visualization. It consists of performing a partial trephination of 70-80% of corneal thickness, followed by a limbal paracentesis incision used to evacuate the aqueous, or to inject air and fluid inside the anterior chamber. The corneal stroma is removed in layer using a bevel-up crescent knife, but the dissection of deeper layers becomes more difficult as DM is approached.52 Subsequently in 1984, Archila53 introduced a new technique of dissection with intrastromal air injection, which is considered the predecessor of other techniques of maximum depth dissection. It provided the injection of air into the corneal stroma until it becomes opaque in such a way to create a deep plane of dissection. After a partial trephination, the wound is deepened with a sharp crescent or a blunt spatula down to the DM, which appears as a clear dark area. The manual dissection of the stroma can be repeated as long as micro bubbles are visible to be sure that there is still a layer of stroma that protects the DM against the perforation. A full-thickness donor button including the DM and endothelium is then positioned in the recipient bed with interrupted sutures. Corneal emphysema provides good contrast, but the baring of DM is still a problem.53In 1997 Sugita and Kondo41 used a technique combining air and fluid injection. A saline solution is injected in a small depression, which is created in the deeper stroma after a partial trephination. This procedure helps to achieve a cleavage plane over DM so that the loosened tissue can be removed in thin layers with forceps and scissors. The DM is recognized from its shiny and smooth appearance. This was later supported by Panda and Singh,54 who compared the efficacy of 3 adjunctive agents to facilitate recipient bed intralamellar dissection (air, hydroxypropylmethylcellulose, and balanced salt solution) demonstrating that the hydro delamination with saline solution is the easiest technique to perform.In the development of DALK, a research line other than the techniques described up to now was disclosed by Tsubota et al55 in 1998. His “divide-and-conquer technique” differs from the others because the DM’s deep dissection is not performed by injecting any substances, and it is practiced completely manually. It originally derives from the application of cataract phacoemulsification technique to the deep LK. The corneal stroma is divided into 4 quadrants to facilitate lamellar dissection at approximately 70% deep, until the DM is exposed in the central area. This way it increases the repeatability and the standardization of the procedure.55 Manche et al56 described a technique, in which viscoelastic was forced into a previously made stromal pocket using a 25-gauge cannula, hoping the viscous material would dissect the DM from the overlying stroma. In the same year, Melles et al57 proposed a variation of this technique by adding the injection of air into the anterior chamber before the stromal dissection with viscoelastic to optimally highlight the cannula’s position, which contained the substance in the pre-descemetic plan. Over the years, this technique was performed, and is still proposed, by several authors43,58-60 with encouraging results.After his first attempt of baring the DM in 2002, Anwar39 described the “big-bubble technique,” as a faster and more reliable way of separating the stroma from the DM. In this surgical technique, the cornea is trephined approximately 60-80% deep using a suction trephine and a 27 or 30-gauge needle. Attached to an air-filled syringe, it is inserted into the deep stroma through the bottom of the trephination groove. The separation of DM from the corneal stroma, characterized by a circular area with a dense white border, is caused by forceful injection of air. A keratectomy, anterior to the big-bubble, is carefully performed so as not to accidentally breech the bubble. Then, the bubble is pierced near the center of the cornea, and an opening in the anterior wall of the air-pocket is formed. The residual layers of stroma are firstly lifted with an iris spatula, then severed with a blade, and excised with scissors (Figures ​Figures33 & 4).61 Similar results have been reported from other case series62-67 of the big-bubble DALK technique for keratoconus. Ghanem et al68 pointed out that bubble formation is the key to decrease the risk of perforation in DALK, especially when a pachymetry-guided intrastromal air injection (pachy-bubble) is performed. However, Yao69 asserting that the many procedures developed for performing DALK are time-consuming and technically difficult, introduced their technique of stromal hooking with viscoelastic detaching process. It consists of creating a pocket in the recipient bed by means of a 3-quarter trephination, followed by a peeling of the remnant stroma to approach DM along the trephined margin in the area between 11 o’clock and one o’clock by the aid of a golf-shaped knife. In this area, the residual stromal fibers are hooked and lifted by forceps with a tip of concaved teeth for the consequent exposure of the DM. A 27-gauge cannula connected to a syringe containing viscoelastic is inserted in the pocket between the stroma and the DM, and the injection of viscoelastic material allows to get the detachment process. In cases where the primary exposure has not exactly reached the layer of the DM, a secondary hooking-detaching procedure can be performed.69 The same procedure was subsequently performed on 75 keratoconus eyes, which showed fewer postoperative complications compared with those who underwent PK.4Open in a separate windowFigure 3Biomicroscopic examination showed a clear and well integrated lamellar graft in a 35-year-old keratoconus patient who had descemetic deep anterior lamellar keratoplasty (big-bubble technique) 6 months previously. Two double-running 12 bites sutures are present.Open in a separate windowFigure 4Anterior segment Fourier-domain optical coherence tomography image 2 years after deep anterior lamellar keratoplasty in a 22-year-old keratoconus patient. A healthy epithelium, the edge of the graft and the interface between donor and recipient cornea (arrows) are notable.Recently, a distinct layer of corneal collagen, the Dua’s layer has been described, beyond the last row of keratocytes, which is thin but tough, and seems to provide a cleavage plane during the DM baring procedure. This observation suggests that the big-bubble cleaves off a distinct layer at the posterior surface of the corneal stroma, which is not residual stroma.70 Farid and Steinert71 in 2009, described a new approach combining big-bubble DALK with the femtosecond laser zigzag incision, in which the matching and interlocking donor-host wound increases surface area, and create a customized donor-host match. In a zigzag incision, the laser creates an angled posterior cut, a lamellar ring cut in midstroma, and an angled anterior cut, each of which intersects. According to Farid and Steinert,71 this type of wound allows rapid healing and good biomechanical stability. Furthermore, the laser cut allows an exact cut depth within 70 µm of the DM, allowing more precise placement of the air needle. Buzzonetti et al72,73 confirmed that the femtosecond laser could standardize the big-bubble technique in DALK, reducing the risk of intraoperative complications and allowing good refractive outcomes. Hoping to achieve higher success in attaining the big-bubble and lower rates of DM perforation, Tan and Mehta74 further modified Anwar’s original technique. Their innovation was to precede the formation of the bubble by the manual removal of the anterior half of the stroma after partial trephination. This way, they had the advantage of reducing the risk of perforation. When immediately entering with the needle into the deeper layers of the stroma, they introduced the needle more centrally, thus allowing for a more centralized bubble.     

Stapled hemorrhoidopexy – Initial experience from a general surgery center

Background

Stapled hemorrhoidopexy is gaining popularity as a relatively painless alternative to conventional excisional hemorrhoidectomy. The initial experience from a service hospital is presented along with review of literature.

Methods

40 cases were managed by stapled hemorrhoidopexy (SH) over a period of two and half years. The primary outcome measures assessed were the analgesic requirement in the post-operative period and the time taken to resume Activities of Daily Living (ADL). Additional outcome measures studied were; presence of bleeding per rectum, prolapse of mass per rectum, incontinence to flatus/stool, post-operative urinary retention, requirement of a repeat procedure, presence of post-operative anal stenosis, and residual external haemorrhoids at 1 week, 1, 3 and 6 months and 1 year after surgery.

Results

40 patients with grade II, III and IV hemorrhoids underwent SH under spinal anesthesia. In the first 24 h 17 patients required a single dose of Injection Diclofenac Sodium while 19 patients required two doses and 4 patients had to be given three doses. 14 patients (35%) achieved Katz Index of Independence in Activities of Daily Living score of 6 on the first post-operative day and another 17 (42.5%) on the second post-operative day. By the fourth post-operative day all patients had achieved a score of 6. One patient had a rectal perforation as a complication of the procedure and another required a second procedure for excision of thrombosed external hemorrhoids.

Conclusion

Stapled hemorrhoidopexy is associated with less post-operative pain and early resumption of ADL. Although the procedure appears simple to perform, it can be associated with serious complications and still cannot be considered the standard of care for the operative treatment of internal hemorrhoids.     

Why do obstetric patients go to the ICU? A 3-year-study

Background

Pregnant women are at risk to develop complications due to illness related to pregnancy or due to aggravation of pre-existing disease. These patients also require critical care and ICU admissions in some cases. To determine the current spectrum of diseases in an obstetric population resulting in admission to the intensive care unit (ICU) at a tertiary care hospital.

Methods

A retrospective case series study and analysis of data from obstetric patients admitted for critical care management.

Results

0.26% of the total obstetric patients admitted to the hospital required ICU admissions. 46% of patients were admitted to ICU for ventilator support. Pre-eclampsia and obstetrical hemorrhage were the common diagnosis for these patients.

Conclusion

Critically ill obstetric patients require a team approach of the obstetrician, anesthesiologist and intensive care specialist for the optimal care of these patients.     

Liver transplantation in Indian Armed Forces—initial experience

Background

This study retrospectively analyses the initial experience of liver transplantation (LT) in the Indian Armed Forces.

Methods

Fifty-three patients underwent LT at Army Hospital (R&R) Delhi Cantt. between March 2007 and March 2011. Of these 35 patients underwent deceased donor liver transplantation (DDLT) and living donor liver transplantation (LDLT) was carried out in 18 patients. The surgical techniques, complications and mortality were analysed.

Results

A high consent rate of 35.9% for organ donation was achieved by the Armed Forces Organ Retrieval and Transplantation Authority (AORTA). Biliary complications occurred in five patients (9.4%). However, most of them could be managed by endoscopic interventions. Hepatic artery thrombosis (HAT) occurred in five patients (9.4%). Of these, two DDLT grafts were revascularised following HAT, by creating extra-anatomic arterial conduits with excellent outcome. The overall mortality was 18.8% (n = 10). There was no significant difference in the overall complications or mortality in patients undergoing DDLT or LDLT.

Conclusion

The overall survival and morbidity in this study is comparable to those from other centres. Urgent revascularisation of grafts following HAT should be attempted as it can salvage grafts with satisfactory outcome. There is a reduction in the incidence of biliary complications with refinements in surgical techniques.     

Perinatal outcomes among children born by assisted reproductive techniques—a hospital-based case control study

Background

Widespread use of assisted reproductive techniques (ART) has raised major concern about the outcome of resulting pregnancies, as well as about the health of the newborn children. The ART conceived pregnancies have an increased risk for prematurity and low birth weight (LBW). The risk of congenital malformations among children conceived via ART is contemplated to be high. The present case control study was conducted with an aim of analysing perinatal outcomes of children born by ART in the Indian context.

Methods

The case control study was conducted in the Army Hospital (Research and Referral), New Delhi. It included 82 cases and 164 controls. The data was collected on maternal and newborn characteristics among cases and controls. Perinatal outcomes were compared between ART conceived and spontaneously pregnant women in terms of birth weight, preterm, multiple pregnancies, and neonatal complications.

Results

Significant difference was observed in terms of the preterm delivery, birth weight, neonatal complications between the cases and the controls. The adjusted odds ratio (OR) was 21.6 (95% confidence interval [CI] 4.3, 112.9) for preterm delivery; 6.0 (95% CI 1.1, 43.8) for multiple pregnancy and 3.2 (95% CI 1.5, 7.0) for caesarean section. The risk of LBW and neonatal complications was heavily confounded by preterm delivery and multiple pregnancies, the adjusted OR being 0.9 and 1.0, respectively.

Conclusion

Increased risk of preterm delivery, multiple pregnancies and caesarean section was associated with ART. The LBW was confounded by preterm delivery and multiple pregnancies.     

Chinese medicine formula HB01 for treating exudative age-related macular degeneration: a 6 consecutive months of clinical observation study

Objective

To evaluate the clinical efficacy of Chinese medicine formula HB01 on exudative age-related macular degeneration (AMD) patients who received HB01 for 6 months by analyzing relative indexes.

A study of haematological and bone marrow changes in symptomatic patients with human immune deficiency virus infection with special mention of functional iron deficiency,anaemia of critically ill and haemophagocytic lymphohistiocytosis

Background

Haematological abnormalities are among the most common complications of HIV. These involve all lineages of blood cells. Bone marrow studies form integral part of complete workup of the HIV positive patients specially when they present as case of pyrexia of unknown origin (PUO), refractory anaemia and pancytopenia.

Method

55 HIV infected symptomatic patient requiring bone marrow examination were included in the study. Relevant clinical history, baseline haematological investigations including full blood count, CD4 cell counts using flow cytometry were recorded.

Results

Median ANC values in males were found to be significantly lower than females (p = 0.046). CD4 cell count statistically significantly correlated with age, TLC, ANC & platelet count. Anaemia was present in 45 patients and out of which 66.66% patients had normocytic normochromic anaemia. Iron deficiency anaemia was present in (37.77%) patients and anaemia of chronic disease in (62.22%) patients. 2 patients had anaemia of the critically ill.Two patients had non-Hodgkin's lymphoma (NHL) and showed lymphoma deposit in the bone marrow. Gelatinous degeneration was seen in 3 patients. Ill formed epithelioid cell granulomas were seen in 7 cases, and 2 cases were positive for acid fast bacilli (AFB). Haemophagocytosis was seen in 8 cases; two cases later diagnosed as a case of infection induced HLH. Leishmania donovani (LD) bodies seen in 2 cases.

Conclusions

Bone marrow study is an important investigation in HIV infected symptomatic patients with peripheral haematological abnormalities.     

Perforator flap: A novel method for providing skin cover to lower limb defects

Background

Soft tissue defects in the lower limb pose a formidable challenge due to lack of reliable local flap options. Due to thin non-expendable soft tissues and predisposition to massive edema formation, even small defects become problematic. Perforator flaps represent the latest trends in soft tissue coverage. The aim of this study was to evaluate the effectiveness of perforator flaps as coverage of soft tissue defects in lower limbs.

Material and methods

A series of patients with soft tissue defects of various etiologies in the lower limb were treated using perforator flap at a tertiary care service hospital. Six were free flaps and fourteen pedicled flaps. Doppler ultrasound was used to identify recipient and donor vessels pre-operatively.

Results

Only one flap was lost due to venous congestion. In another case of carcinoma penis there was delayed healing due to persistent lymphorrhoea as a result of post-operative status following lymph node dissection and radiotherapy. Fifteen donor sites required split skin grafting, as they could not be closed directly.

Conclusion

Perforator flaps are a reliable option for closure of soft tissue defects of lower limb irrespective of size, location and depth. There is minimal donor site morbidity. It has the advantage of rapid dissection, flap elevation and reliable skin territory. As no special equipment is required it can be replicated in smaller centers also.