不同方法确定动情周期及动情期生殖器官结构

目的 为确定最佳合笼时间,对两种常见鉴定小鼠动情周期方法进行比较,并确定处于动情期的子宫内膜和输卯管的形态结构,为动物生殖发育及胚胎研究提供基础技术指导.方法 对43只昆明小鼠分别应用阴道脱落细胞检查法、宫颈黏液结晶法,确认小鼠动情周期,与子宫、输卵管和卵巢做成石蜡切片,HE染色后镜下观察的结果进行统计分析比较,同时对处于动情期的小鼠子宫内膜和输卵管进行HE染色.结果 阴道脱落细胞检查法所确定小鼠动情周期与子宫镜检所见确定的动情周期中,在动情期的判定上吻合率较高;小鼠的阴道脱落细胞在动情周期各个时期有不同的形态特点,处于动情期的小鼠的子宫内膜和输卵管的形态与处于非动情期的小鼠有明显变化.结论 阴道脱落细胞染色检查确定动情期比宫颈黏液结晶法更为可靠;小鼠激素水平的变化使阴道上皮细胞发生周期性变化,动情期小鼠输卵管和子宫内膜发生相应改变分别为受精和着床做准备.     

NOD/SCID雌性小鼠动情周期的观察

目的：观察NOD/SCID小鼠动情周期及卵巢切除术后阴道涂片的变化。方法：连续观察9天NOD/SCID鼠,每日进行两次阴道涂片,计算动情周期时间及发生率。卵巢切除后,阴道涂片并观察阴道涂片变化。结果：NOD/SCID小鼠动情周期为4-6天。有规律动情的小鼠占80%。阴道口状态与动情周期无明显相关。卵巢切除术后,阴道涂片呈动情后期或动情间期改变。结论：采用阴道脱落细胞涂片法可判断NOD/SCID雌性小鼠的动情周期及特点,NOD/SCID雌性小鼠卵巢切除手术有效。     

宫腔注射无水乙醇对小鼠动情周期的影响

目的探讨宫腔内注射无水乙醇对小鼠动情周期的影响。方法利用阴道脱落细胞染色法选择动情期规律的小鼠20只,宫腔注射无水乙醇,术后第一个动情周期内每日测定其动情周期,随机择10只在第二个动情周期每2 h测一次,观察动情周期的改变程度。结果实验小鼠宫腔注射无水乙醇后动情周期整体发生大幅度延长,且动情期、动情前期等各期时间均发生了不同程度的紊乱。结论本实验通过观测无水乙醇损伤子宫后小鼠动情周期发生紊乱的现象,为临床无水乙醇的使用提供一定的实验基础。     

小鼠生物净化中的动情周期判定及超数排卵

目的 准确判定小鼠动情周期的变化,并在动情周期的不同阶段诱导超数排卵,探索最佳的超排时间,以提高生物净化的成功率.方法 C57BL/6J小鼠每日08：00至13：00,取阴道脱落细胞进行涂片,观察脱落细胞中白细胞、有核细胞和角质化细胞的比例及变化的规律,确定小鼠的动情周期.据判定的动情周期的各阶段,腹腔注射外源激素分别诱导超数排卵后,于输卵管中取卵母细胞计数,并统计分析.结果 根据阴道涂片确定小鼠动情前期（包括过渡期）、动情期（Ⅰ、Ⅱ期）、动情后期和间情期.4～6周龄C57BL/6J小鼠诱导超数排卵的最佳时期是动情前期,平均可获得23.20枚卵母细胞,显著高于其他实验组（P＜0.001）.结论 据阴道涂片观察,C57BL/6J小鼠单个动情周期持续4～10d,动情周期内不同时间段超排效果也不同.     

电针对血管性痴呆小鼠海马神经元c-fos表达的影响

目的观察电针对血管性痴呆(VD)小鼠海马神经元凋亡及相关基因cfos表达的影响。方法以反复脑缺血再灌注法复制VD小鼠模型,电针肾俞、膈俞和百会穴,对照组灌胃喜得镇,分别于治疗1d和7d后,以FCM法检测小鼠海马细胞凋亡率、cfos表达。结果术后1d,模型小鼠海马细胞凋亡率(6.86±1.10)较假手术组(3.59±0.70)明显增高(P<0.01),术后7d(13.49±1.72)较术后1d还高(P<0.01)。术后1d,模型小鼠海马细胞cfos表达(1.08±0.33)较假手术组(1.00±0.25)有升高趋势(P>0.05),7d时(2.65±0.29)则显著升高(与术后1d和假手术组比较,均P<0.01)。电针在两个时点均可上调小鼠海马细胞cfos的表达(2.26±0.31,3.76±0.41),降低细胞凋亡率(3.68±1.48,6.36±1.52),与模型组相比均差异有显著性(P<0.01)。结论电针具有抑制细胞凋亡、保护神经元的作用,这可能是电针改善VD小鼠智能的作用机理之一。     

乙二醇、丙二醇对胎儿卵巢冻存效果的影响

目的比较1.5mol/L的乙二醇和丙二醇两种低温保护剂对胎儿卵巢组织的保存效果。方法采用“一步降温法”对24～28周水囊引产的胎儿卵巢进行冻存,将解冻后的胎儿卵巢组织移植于去势裸鼠的双肾被膜下,分别于移植后2、4及8周取材,通过电镜观察及移植后的组织学、受体鼠血清雌激素水平测定和阴道脱落细胞涂片判断组织细胞活性。结果乙二醇对卵泡形态结构的保存优于丙二醇,后者在卵巢的部分区域可见不可逆性的冷冻损伤;乙二醇组动情周期恢复率为33.3%,移植后动情周期开始天数为(15.00±5.0)d,而丙二醇组动情周期恢复率为16.7%,移植后动情周期开始天数(23.50±6.26)d。移植2周后乙二醇组与丙二醇组的移植物存活率分别为83.3%、66.7%,两组受体鼠血清雌激素水平之间的差异具有统计学意义(P<0.05),异种移植8周后两组移植物的存活率分别为58.3%、33.3%。结论以1.5mol/L的乙二醇作低温保护剂行快速冷冻效果优于1.5mol/L的丙二醇。     

不同剂量顺铂诱导小鼠卵巢功能早衰程度的差异

目的比较不同剂量顺铂诱导小鼠卵巢功能早衰(POF)的差异,提供适宜的POF研究模型。方法 6～8周雌性ICR小鼠腹腔注射不同剂量顺铂,观察一般情况、动情周期和卵巢组织病理学变化,计算小鼠死亡率。结果顺铂按首日给予3mg/kg,再连续6d腹腔注射2mg/kg,小鼠除体重减轻外,一般状况尚可,卵巢损伤相对较轻,停药后14d,50%小鼠动情周期恢复正常,显微镜下可见较多不同发育时期的卵泡及成熟卵泡,小鼠死亡率10%;连续7d给予顺铂3.0mg/kg,小鼠出现精神萎靡、食欲下降、动情周期紊乱,停药后14d动情周期未恢复,且小鼠死亡率达70%,卵巢组织中可见较多闭锁卵泡,基本见不到发育成熟的卵泡。3d给予顺铂3mg/kg,再给予顺铂2.0mg/kg 4d,小鼠的一般状况、动情周期与组织病理学变化介于前两个方案之间,20%的小鼠死亡。结论小鼠前3d和后4d分别给予3mg/kg和2mg/kg顺铂腹腔注射,在高存活率基础上能更好地形成POF病变,是最为可行的诱导POF方案。     

小鼠阴道涂片技术及其染色方法的研究

目的比较研究棉签法、生理盐水冲洗法进行阴道涂片,HE染色法、瑞氏染色法、巴氏染色法染色对C57BL/6小鼠阴道涂片细胞组织染色的效果,为小鼠动情周期的确定寻找便捷、有效的检验方法。方法对10只C57BL/6雌性小鼠分两笼饲养,每日10∶00用棉签法、生理盐水法进行阴道涂片,连续15 d,并使用HE染色法、瑞氏染色法、巴氏染色法,观察记录。结果连续实验生理盐水法优于棉签法,HE染色染料易于获得;瑞氏染色分型清楚,染色佳;巴氏染色形态清晰,分色明显,透明度好。结论大样本量旨在判断动情周期的发生与改变实验应采用瑞氏染色法,可辅以巴氏染色以增强实验动物数据资料的可靠性。     

豫医无毛小鼠寿命观察

目的 :通过对豫医无毛小鼠寿命的观察 ,探讨突变基因对无毛小鼠寿命的影响。方法 :在正常生产繁殖状态下 ,随机选取无毛小鼠雌雄各 50只 ,杂合子有毛小鼠雌雄各 2 5只 ,饲养条件、营养状况、实验方法等均相同 ,观察每只小鼠自然衰老的过程 ,并计算出各自的寿命。结果 :纯合子无毛小鼠平均寿命雌性为 (30 3 .3± 35 .0 )d、雄性为 (2 61 .6± 41 .2 )d ,杂合子有毛小鼠平均寿命雌性为 (382 .7± 35 .7)d、雄性为 (353 .0± 37.2 )d。纯合子无毛小鼠与杂合子同性别有毛小鼠寿命相比差异有统计学意义 (P <0 .0 1 )。结论 :突变基因可能对无毛小鼠的寿命有影响 ,豫医无毛小鼠可作为衰老模型供科研应用     

β-巯基乙醇对培养胎儿卵巢组织异种移植效果的研究

目的探讨β-巯基乙醇(β-ME)对体外培养的卵巢组织的保护作用,寻找提高胎儿卵巢组织异种移植存活率的有效方法。方法取中晚期引产的死亡女胎,分别进行新鲜移植(对照组)或将卵巢组织在不含β-ME(培养对照组,β-ME0)、含100μMβ-ME(培养组,β-ME100)的培养液中新鲜培养3d后移植到裸鼠体内,从动情周期的恢复情况、组织学变化等方面比较各组移植物的活力。结果(1)β-ME0、β-ME100及对照组裸鼠动情周期的恢复率分别是62.5%、87.5%和33.3%,β-ME100组与对照组比较差异有统计学意义(P<0.05)。β-ME0、β-ME100组和对照组移植后动情周期开始天数为(25.20±4.49)d、(23.5±5.79)d和(12.75±1.5)d,两培养组均明显长于对照组(P<0.05);(2)异种移植后的组织学观察可见到存活移植物内有不同发育阶段的卵泡;卵泡计数β-ME100组(16.4±3.7815个/高倍视野)与对照组(4.0±2.8000个/高倍视野)比较差异有统计学意义,P<0.05。结论胎儿卵巢组织异种移植后卵泡可继续发育,经培养可提高移植物的活力,β-ME有利于提高移植物的存活率,促进卵泡的发育。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.