Peripheral blood and bone marrow lymphocyte subsets in patients with multiple myeloma

Summary A panel of monoclonal antibodies (McAb) from the Wu series was used to characterize lymphocyte subsets of peripheral blood (PB) from 9 patients with multiple myeloma (MM) by means of indirect immunofluorescence assay. In 7 of these patients bone marrow (BM) cells were also studied. Abnormal distribution of T lymphocyte subsets was observed in these patients, and the alterations were quite similar in both PB and BM. A significant increase in the proportion of WUT ₈ ⁺ cells (P<0.00l) and a reduced WuT₄/WuT₈ ratio (P<0.001) were found in patient group. In both PB and BM of patients, WuT ₁₀ ⁺ cell counts were significantly higher than normal (P<0.00l). There was a good correlation between BM WuT ₁₀ ⁺ cells and BM myeloma cells (r= 0.76, P<0.05), whereas no correlation was found between PB WuT ₁₀ ⁺ cells and BM myeloma cells. The significance of abnormal T-cell subset distribution and elevated WuT ₁₀ ⁺ cells in MM is discussed.     

中西医结合治疗对Graves病患者外周血T、B淋巴细胞亚群的影响

目的：研究中西医结合治疗对Graves病患者外周血T、B淋巴细胞亚群及部分协同刺激分子表达的影响。方法：运用流式细胞术测定20例Graves病初发患者、11例他巴唑治疗患者（西药组）、16例他巴唑合用中药五岳抗甲丸治疗患者（中西医结合组）及16例正常对照外周血淋巴细胞CD19、CD40、CD80、CD3、CD4、CD8、CD28等分子的表达水平。结果：Graves病初发患者CD19⁺、CD40⁺、CD19⁺ CD40⁺、CD80⁺细胞比率高于正常对照组(P＜0.01或P＜0.05)，CD3⁺CD8⁺细胞比率降低(P＜0.05)，CD3⁺CD4⁺/CD3⁺CD8⁺比值高于正常对照(P＜0.05)；西药组患者CD80+细胞恢复正常，CD3⁺CD4⁺细胞比率及CD3⁺CD4⁺/CD3⁺CD8⁺比值高于正常对照(P＜0.05，P＜0.01)，而CD3⁺CD8⁺及CD8⁺CD28⁺细胞比率低于正常对照(P＜0.01，P＜0.05)；除CD80+细胞比率高于正常对照外，中西医结合组所测各种分子的表达同正常对照组比较，差异无统计学意义；同西药组比较，中西医结合组CD3⁺CD4⁺细胞降低(P＜0.01)，CD3⁺CD8⁺及CD8⁺CD28⁺细胞比率升高(P＜0.01，P＜0.05)， CD3⁺CD4⁺/CD3⁺CD8⁺比值降至正常水平(P＜0.01)。结论：Graves病患者体液免疫处于较高水平，存在细胞免疫调节失衡，中西医结合治疗较单纯西药治疗更有利于患者免疫状态的恢复。     

男男同性恋人群HIV-1早期感染者T细胞活化水平分析

目的 评价人类免疫缺陷病毒（human immunodeficiency virus,HIV）早期感染者T细胞活化水平,寻找HIV早期感染者疾病进展的预测指标.方法 以2010年1月至2010年12月间首都医科大学附属北京佑安医院男男同性恋（men who have sex with men,MSM）队列筛查出的25例HIV早期感染者为研究对象.取冻存的HIV早期感染者和未感染者的外周血单个核细胞（peripheral blood mononuclear cells,PBMCs）,流式细胞术检测CD4＋T细胞、CD8＋T细胞表面活化标志CD38 和人类白细胞抗原DR（human leukocyte antigen DR,HLA-DR）的表达.Pearson相关法分析HIV早期感染者CD4＋T细胞、CD8＋T细胞活化与CD4细胞数、血浆病毒载量的相关性.结果 与未感染者相比较,HIV早期感染者HLA-DR＋CD4＋T细胞、CD38＋HLA-DR＋CD4＋T细胞的比例显著增加（P〈0.05）,而CD38＋CD4＋T细胞的比例无明显变化.HIV早期感染者CD38＋CD8＋T细胞、HLA-DR＋CD8＋T细胞和CD38＋HLA-DR＋CD8＋T细胞的比例较未感染者均显著增加（P〈0.05）.然而,感染时间不同的HIV早期感染者之间相比,CD4＋T细胞和CD8＋T细胞的活化水平差异无统计学意义.此外,CD38＋ CD8＋T细胞、CD38＋HLA-DR＋CD8＋T细胞的比例与CD4细胞数呈显著负相关,而与血浆病毒载量呈显著正相关.结论 CD38＋ CD8＋T细胞、CD38＋HLA-DR＋CD8＋T细胞的比例有可能作为HIV早期感染者疾病进展预测的指标.     

人乳牙牙髓干细胞对干燥综合征患者CD4+T细胞的免疫抑制效应

目的 探讨在体外人乳牙牙髓干细胞(stem cells from human exfoliated deciduous teeth, SHED)及其联合IL-6抗体对干燥综合征患者(Sjgren syndrome, SS)外周血活化的CD4⁺T细胞的免疫抑制效应。方法 培养并鉴定SHED;流式细胞术检测健康者和SS患者外周血CD4⁺T细胞中Th1、Th2、Th17、Treg及Tfh的比例;将健康者和SS患者PBMC各自单独培养或与SHED共培养,ELISA法检测培养上清液中IL-6浓度,在各组中添加或不添加IL-6抗体,72h后流式细胞术检测CD4⁺T细胞增殖情况。结果 SS患者外周血CD4⁺T细胞中Th17及Tfh比例较健康者升高(P<0.05);健康者与SS患者共培养组的Th17比例显著降低(P<0.001),SS患者共培养组的Treg比例有所升高(P<0.05);在SS患者共培养组中添加IL-6抗体,可显著降低CD4⁺T细胞中Th2的比例(P<0.05)。结论 SHED可抑制SS患者CD4⁺T细胞的增殖,降低SS患者CD4⁺T细胞中Th17的比例且增加Treg的比例;IL-6抗体可降低与SHED共培养的SS患者PBMC中CD4⁺T细胞Th2亚型比例。     

Changes in the level of IL-2, T cell subsets and the function of T suppressor cells in patients with schistosomiasis japonica

Summary The level of interleukin-2 (IL-2) produced by peripheral blood mononuclear cells in vitro, T cell subsets and the function of T suppressor cells (Ts) in patients with schistosomiasis japonica were investigated. It was found that the level of IL-2 induced by schistosomal antigens was significantly high and positively related to the percentage of CD4⁺ cells. In the groups of chronic and advanced schistosomiasis japonica, the level of IL-2 induced by schistosomal antigens was markedly lower than that in the group of acute schistosomiasis japonica, but significantly higher than that in the group of normal controls. The IL-2 level was negatively related to the percentage of CD8⁺ T cells and the ratio of CD4⁺/CD8⁺. The level of IL-2 induced by PHA was greatly reduced in the group of advanced schistosomiasis japonica. The changes in the level of IL-2 and its relationship with T cell subsets and the function of Ts are also discussed.     

甲状腺相关性眼病患者外周血CD4+、CD8+T细胞百分比及细胞表面程序性死亡蛋白1表达的改变及其意义

目的 观察甲状腺相关性眼病(TAO)患者外周血单个核细胞(PBMC)中CD4+、CD8+T细胞百分比及细胞表面程序性死亡蛋白1(PD-1)的表达水平,探索这两者变化在TAO发病机制及疾病诊断中的意义.方法 收集21例TAO患者、21例Graves病(GD)无眼病患者及20例健康对照者资料,采用流式细胞术检测所有受试者PBMC中CD4+、CD8+T细胞百分比及其表面PD-1的表达率,比较组间差异,分析其与患者病程、甲状腺功能、甲状腺相关抗体及TAO疾病活动性和严重程度之间的关系.结果 (1) TAO患者PBMC中CD4+、CD8+T细胞百分比分别为(35.9±14.5)％、(22.2±8.4)％,GD患者分别为(33.1±13.0)％、(18.6±9.2)％,均高于健康对照者[(17.4±7.4)％、(7.7±4.8)％,P＜0.01].(2) TAO及GD患者PBMC中CD4+T细胞表面PD-1表达率分别为(8.3±6.4)％、(28.0±26.6)％,均低于健康对照者[(52.2±28.6)％,P＜0.01.P＜0.05],且TAO患者低于GD患者(P＜0.05);TAO患者PBMC中CD8+T细胞表面PD-1表达率低于健康对照者r(12.7±13.4)％vs(38.2±24.2)％,P＜0.01].(3) TAO患者PBMC中CD8+T细胞百分比与病程呈正相关(r=0.478,P＜0.05).(4) TAO患者活动期组与非活动期组、轻度组与中重度组间CD4+、CD8+T细胞百分比及其表面PD-1表达率差异均无统计学意义(P＞0.05).结论 TAO患者PBMC中CD4+、CD8+T细胞百分比及细胞表面PD-1表达存在异常,可能参与TAO的自身免疫过程.     

Prognostic Significance of Tumor-infiltrating CD8+ or CD3+ T Lymphocytes and Interleukin-2 Expression in Radically Resected Non-small Cell Lung Cancer

Background:Altered immunoresponse is associated with tumorigenesis and cancer progression.This study assessed the levels of tumor-infiltrating CD3+ or CD8+ T lymphocytes and interleukin-2 (IL-2) protei...     

Expression of costimulatory molecules B7/CD28 in systemic lupus erythematosus

Summary The expression of the constimulatory molecules B7/CD28 in peripheral blood mononuclear cells (PBMC) of the patients with systemic lupus erythematosus (SLE) and its relation to the pathogenesis of SLE were studied. The expression of the costimulatory molecules in PBMC in 30 patients with active SLE and 20 cases of healthy controls was detected by using the techniques of immunofluorescence and flow cytometer. The result showed that the expression percentage of CD28', CD4' CD28 in T cells of PBMC from the patients with SLE decreased significantly as compared with that in healthy control group, while the expression percentage of CD80⁺, CD19⁺ CD80⁺ in B cells was significantly increased than that in healthy control group (P<0.01). It suggested that the abnormal expression of costimulatory molecules B7/CD28 played a role in the pathogenesis of SLE. HU Shaoxian, female, born in 1962, Associate Professor This project was supported by a grant from Hubei Provincial Natural Sciences Foundation of China (No. 2000j053).     

慢性丙型肝炎患者DNT细胞及T细胞亚群的研究

目的探讨外周血中CD3+CD4-CD8-(double negative T,DNT)细胞及T淋巴细胞亚群在慢性丙型肝炎(chronichepatitis C,CHC)患者中的表达及意义。方法利用流式细胞仪对92例CHC患者、93例正常健康人外周血中的DNT细胞及T细胞亚群进行检测,并对比分析两者的差异。结果 CHC患者DNT细胞百分比(8.57%±6.16%)高于正常对照组(6.78%±4.50%)(P<0.05),其绝对数在两者间比较,差异无统计学意义(P>0.05)。CHC患者CD4+%,CD4+/CD8+比值低于正常对照组(P值均<0.001),CD8+%高于正常对照组(P<0.001)。结论 CHC患者DNT细胞百分比显著升高及T淋巴细胞亚群比例改变,可能是丙型肝炎病毒感染慢性化的重要原因之一。     

自体树突状细胞疫苗联合细胞因子诱导的杀伤细胞治疗胆系肿瘤的临床疗效观察

目的 观察自体树突状细胞(DC)疫苗联合细胞因子诱导的杀伤细胞(CIK细胞)治疗胆系肿瘤的临床疗效。 方法 采集患者外周血单个核细胞,采用多种肿瘤抗原刺激后进行扩增和培养,收获成熟DC制成疫苗进行皮内注射,同时联合自体CIK细胞静脉回输,观察85例胆系肿瘤患者治疗前后的临床疗效、免疫功能、不良反应及生存时间。 结果 85例胆系肿瘤患者经自体DC-CIK细胞治疗后,临床症状较前明显改善(P <0.05);临床疗效显著,完全缓解2例(2.3%)、部分缓解14例(16.5%)、疾病稳定54例(63.5%)及疾病进展15例(17.6%);免疫功能提高,治疗后淋巴细胞亚群中CD3⁺、CD3⁺CD4⁺、CD3⁺CD8⁺、CD3⁺CD56⁺及CD4⁺/CD8⁺比值升高,而调节性T细胞降低(P <0.05)。至随访结束,58例患者死亡,2例失访,25例存活,中位生存期为16.5个月(95% CI:12.1~20.9个月)。 结论 自体DC-CIK细胞治疗是胆系肿瘤患者一种安全有效的治疗方法,不良反应少,但相关结论仍有待进一步大样本随机对照研究证实。