Bone Density and Mechanical Properties in Femoral Bone of Swim Loaded Aged Mice

INTRODUCTIONTheincidenceofosteoPorosishasbeenrapidlyincreasinginJapanwiththein-creaseintheagedPOpulation(Orimo,l996).Sinceosteoporosisisoneofthecausesofpoorqualityoflife(QOL)inagedindividualsandalsoacauseofincreasesinmedi-calexpenditures,itisimPOrtanttopreventitsincidence.Itisknownthatweightless-nessorbedrestisassociatedwithboneloss(Luekenetal.,1993).PhysicalexerciseisrePOrtedtobeeffectiveforpreventionofosteoPOrosisinhumans(Aloiaetal.,o895-3988/98CN11-28l6copyright@l998bycirMl978;…     

Effect of Reduqing (热毒清) on Endotoxin-Induced Production of Tissue Factor and Cytokines in Human Whole Blood

lipopolysaccharide,monocytes,mononuclearcellsReduqinginjection(RDQ,originallynamedAnti--inflammatoryAgentNo.6)isamodernChineseherbalpreparationmodifiedfromtherepresentativedecoctionWuweiXiaoduYin(Eapi6$ts).ItconsistsofextractedmaterialsfromFlosLonicerae,Foliumlsatidis,HerbaTaraxaci,andHerbaHouttuyniae,andbelongstothe"heat--clearinganddetoxifying"categoryofChinesemedicine.Inthepasttwodecades,RDQInfectionhadbeenusedinanumberofChinesehospitalsforthetreatmentofacuteabdomenandotheracuteinf…     

EXPERIMENTAL WORK and RESEARCH Effect of Reduqing(热毒清) on TNF-α,IL-1,IL-6,IL-8 and PAF Levels in Endotoxin-Induced DIC Model of Rabbits

ＥＸＰＥＲＩＭＥＮＴＡＬＷＯＲＫａｎｄＲＥＳＥＡＲＣＨＥｆｆｅｃｔｏｆＲｅｄｕｑｉｎｇｏｎＴＮＦ－α，ＩＬ－１，ＩＬ－６，ＩＬ－８ａｎｄＰＡＦＬｅｖｅｌｓｉｎＥｎｄｏｔｏｘｉｎ－ＩｎｄｕｃｅｄＤＩＯＭｏｄｅｌｏｆＲａｂｂｉｔｓ￥ＬＩＭｉｎｇ－ｚｈｅｎ...     

Effects of Modified Sanhuang Decoction (加味三黄汤) Enema on Serum Tumor Necrosis Factor-α and Colonic Mucosa Interleukin-1β, Interleukin-6 Levels in Ulcerative Colitis Rats

Objective:To investigate the effects of Modified Sanhuang Decoction(加味三黄汤,MSD)enema on the serum tumor necrosis factor alpha(TNF-α)and colonic mucosa interleukin-1β(IL-1β),interleukin-6(IL-6)levels in experimental ulcerative colitis(UC)rats.Methods:Forty-five male Wistar rats were randomly divided into 4 groups:normal group(n=12),model group(n=11),salazosulfapyridine(SASP)group(n=11)and MSD group(n=11).The UC model was induced by 2,4,6-trinitrobenzene sulfonic acid(TNBS)/ethanol solution.Rats in the normal group and model group were clystered with 0.9%normal saline,while in the SASP group and MSD group were clystered with SASP and MSD enema,respectively.After drug administration(10 mL/kg body weight,for 7 days),colonic gross changes and colonic mucosa histology were observed,serum TNF-αand colonic mucosa IL-1β,IL-6 levels were tested by enzyme linked immunosorbent assay and radioimmunoassay,respectively.Results:As compared with the normal group,the experimental UC rats,the colonic mucosal damage index scores(CMDIs),histopathological scores(HS)and the serum TNF-a and colonic mucosa IL-1β,IL-6 levels significantly increased(P0.05 or P0.01).In the MSD and SASP groups,the ulcer area significantly reduced,and edema disappeared.The CMDIs,HS,the serum TNF-a and colonic mucosa IL-1β,IL-6 levels in the MSD and SASP groups significantly decreased(P0.05 or P0.01)compared with the model group.The CMDIs in the MSD group were lower than that in the SASP group(P0.05),but there were no significant differences in HS,serum TNF-αor colonic mucosa IL-1β,IL-6 levels between the MSD and SASP groups.Conclusion:MSD enema can improve colonic mucosa impairment and decrease serum TNF-αand colonic mucosa IL-1β,IL-6 levels in experimental UC.     

Protective effects of bactericidal/permeability-increasing protein against hemorrhagic shock and its mechanism

Morbilityandmortalityofgram--negativesepsisremainatahighleveldespitecontinuousapplicationofnew,broad--spectrumandpowerfulantibiotics,whichhasbeenconsideredtoberelatedtobacterialendotoxinwhichcannotbeneutralizedbyantibioticsllj.Therefore,tolookforaneffectiveagenttoantagonizeendotoxinactivitieshasbeenahottopicinthefieldofsurgery.Bactericidal/permeability--increasingprotein(BPI)isarecentlyfoundproteinwhichhasbothbactericidalandendotoxin--binding/neutralizingeffects[Zj.IthasbeendemonstratedthatB…     

let-7/miR-98家族对IL-6的调节作用

目的探索微小RNA对IL-6的调节作用。方法通过生物信息学预测发现let-7/miR-98家族可以调控IL-6基因,采用聚合酶链反应(RT-PCR)和酶联免疫法检测其对IL-6基因表达的影响,采用定点突变和双荧光报告基因系统来验证let-7/miR-98家族对IL-6基因的直接调节作用。结果 miR-98可以抑制IL-6蛋白的表达,miR-98对IL-6 mRNA也有抑制作用,let-7/miR-98可直接调节IL-6基因3’UTR。结论 let-7/miR-98家族可以直接调节IL-6基因的表达,提示let-7/miR-98家族可能成为干预IL-6参与的相关疾病的一种新药物。     

CHEN KEJI(陈可冀)──Outstanding Cardiologist and Gerontologist, A Pioneer of Integrative Medicine

ProfessorChenKen,M.D.,67,hasover40yearsofclinicalexperienceinChinesemedicineandintegratedtraditionalandwesternmedicine.Healsohasadistinguishedclinicalresearchbackgroundincardiology,geriatrics,QingRoyalmedicineandanti--agingherbalmedicines.HehasreceivedseveralnationalMinistryofHealthacademicawardsinChinaincludingthosefortheCoronaryHeartProjectNo.11andaromaticformulafortreatingcoronaryheartdisease,tetramethylpyrazinefortreatingacutecerebralthrombosis,hygenaminefortreatingsicksinussyndrome(…     

Effects of vitamin E on the proliferation and collagen synthesis of rat hepatic stellate cells treated with IL-2 or TNF-α

Objective To study the effects of vitamin E on the proliferation and collagen synthesis of rat hepatic stellate cells treated with interleukin-2 (IL-2 ) or tumor necrosis factor-α (TNF-α). Methods Hepatic stellate cells were isolated from male Sprague-Dawley rats by using modified Friedman’s method. Using the isolated cells cultured and treated with IL-2 or TNF-α, we studied the effects of vitamin E on their proliferation and collagen synthesis through an (3)H-thymidine and (3)H-proline incorporation assay, as well as through observation of these cells under a contrary phase microscope. Results Adding IL-2 increased the both proliferation and collagen synthesis of hepatic stellate cells. Their proliferation was also increased by the addition of TNF-α, although it decreased collagen synthesis. Vitamin E had marked inhibitory effects on the ability of cells treated with IL-2 or TNF-α to reproduce or synthesize collagen. Conclusion Vitamin E can inhibit the proliferation and collagen synthesis of hepatic stellate cells. It is possible that vitamin E affects liver fibrosis through these activities.     

Effect of IFN-γ, IL-4 on proliferation and synthesis of hyaluronic acid and collagen in cultured human retroorbital fibroblasts in vitro

Objective To investigate the cellular immune mechanism of thyroid associated ophthalmopathy (TAO) and provide a basis for treating TAO with cytokine or anti-cytokine agents, we determined whether interferon (IFN)-γ and interleukin (IL)-4, representative cytokines of Th1 and Th2 cells, may have some effect on the development and progression of TAO.Method Retroorbital fibroblasts (RF) proliferation and the synthesis of hyaluronic acid (HA) and type Ⅳ collagen were measured with liquid scintillation and radioimmunoassay. Results IFN-γ stimulated RF proliferation and HA synthesis and had a significant inhibitory effect on type Ⅳ collagen synthesis. IL-4 stimulated proliferation and type Ⅳ collagen synthesis in RF and had inhibitory effect on HA synthesis. When IFN-γ (100U/ml) and IL-4 (1μg/L) were incubated together with RF, they antagonized their respective stimulatory or inhibitory effect on the proliferation and synthesis of HA and type Ⅳ collagen. Thyrotropin (TSH) stimulated RF proliferation and type Ⅳ collagen synthesis in a dose-dependent manner, while only at high dose (100U/L and 200U/L), stimulated RF to synthesize HA. Conclusions IFN-γ, a representative cytokine of Th1 cells, is responsible for the inflammatory process of TAO; whereas IL-4, a representative cytokine of Th2 cells, has some effect on the repair process. IL-4 could antagonize the inflammatory effect of IFN-γ on RF. TSH may have aggravating effect on the pathogenesis of TAO.     

Imaging analysis on the collagen distribution pattern in hypovascular zone of normal supraspinatus tendon

Of the conshtuent muscles of mtator cuff, the suPmpinatus tendon Proves to be a more impoltant one,and tends to subject to l.,ions[1--5]. Many,qulhors POintedout that, in the ~ of I -- I .5 cm close to the insenionof M. suPlaSPinatus, there edests a noticeable hpocularity or relative avasctilallty zone, which is just the anastomosis area of suPI'aSCapulmp ~, subscapuleq arteryand anterior hUmend ci~ex artery, and ProneS tO degenerative ie,io.[6]. The tendon has a collagen pattern oftype…