HPLC测定龙血竭胶囊中二氢查耳酮（龙血素A）的含量

目的：建立HPLC法测定龙血竭胶囊中龙血素A的含量。方法：以C18反相键合硅胶为固定相,1％冰醋酸－甲醇（34：66）为流动相,检测波长为280nm,用外标法定量。结果：龙血素A在0．11－4．39μg范围有良好线性关系,r=0.9998,方法回收率为98．28％,RSD为1．97％。结论：本方法是测定龙血竭胶囊中龙血素A含量的可靠、简便的定量方法。     

高效液相色谱法测定龙血竭滴丸中龙血素B的含量

目的:建立测定龙血竭滴丸中龙血素B含量的测定方法--高效液相色谱法(HPLC).方法:采用反相高效液相色谱法,Diamonsil-C18色谱柱,流动相为乙腈-0.4%磷酸溶液(45∶ 55),检测波长为275 nm,流速为1.0 ml/min,柱温为30 ℃.结果:龙血素B在8.28～99.36 ng范围内与峰面积呈线性关系(r＝0.999 6),该制剂中龙血素B的平均回收率为99.03%,RSD为2.09%(n=5).结论:HPLC法简便,准确,可靠,可用于该龙血竭滴丸的质量控制.     

剑叶龙血树根、茎、叶中血竭成分分析

目的通过对比剑叶龙血树根、茎、叶成分与龙血竭成分间的异同及对剑叶龙血树根、茎、叶中龙血素A、B的测定,寻找龙血竭原料的可能来源。方法薄层色谱法及高效液相色谱技术。结果剑叶龙血树根、茎、叶中均含有微量的龙血素A,分别为:58、53、15μg/g;叶中还含有微量的龙血素B,为2μg/g,而根、茎样品中未能检测到龙血素B。结论从色谱峰的积分面积及保留时间来看,剑叶龙血树根、茎、叶成分与血竭原料有少许的相关性,但差异显著;根、茎、叶3者间的化学组成成分也存在着显著差异,剑叶龙血树根、茎、叶中龙血素量都较低,不能直接用作生产血竭的原料。     

HPLC同时测定龙血竭及其提取物中5个有效成分的含量

目的观察HPLC同时测定龙血竭及其提取物中龙血素A,龙血素B,7,4’-二羟基黄酮,紫檀茋,白藜芦醇的含量的效果,探讨其优势,为研究提供科学依据。方法采用Kromasil 100-5 C18（250mm×4.6mm,5μm）色谱柱;流动相乙腈-1%冰醋酸;流速1.2mL/min;柱温40℃;检测波长龙血素A和龙血素B为270nm,7,4’-二羟基黄酮、紫檀茋和白藜芦醇为300nm。结果 5个有效成分在35min内均形成了基线分离,线性关系良好（r≥0.9997）。龙血竭中龙血素A,龙血素B,7,4’-二羟基黄酮,紫檀茋,白藜芦醇的平均回收率分别为101.9%,95.81%,96.29%,101.7%,102.7%,RSD分别为0.21%,1.4%,0.39%,0.57%,0.33%;龙血竭提取物中龙血素A,龙血素B,7,4’-二羟基黄酮,紫檀茋,白藜芦醇的平均回收率分别为101.2%,97.23%,96.09%,101.0%,102.3%,RSD分别为1.5%,0.91%,1.3%,1.4%,1.3%。结论采用HPLC同时测定法效率高、操作简便、准确率高,重复性和稳定性良好,大大提高了龙血竭及其提取物的研究效率,对其学科发展有重要意义。     

龙血竭中龙血素A与龙血素B在大鼠体内的组织分布研究

目的研究龙血竭中龙血素A、龙血素B在大鼠体内的组织分布情况。方法按100 g大鼠给予龙血竭250 mg,灌胃给药,用HPLC法测定不同时间点的组织浓度。结果龙血素A、B在肝脏及肾脏浓度最高,然后依次是肺、脾、心、脑。结论龙血素A、B在大鼠体内主要分布于血流量大的脏器。     

龙血竭缓释滴丸在家兔体内药动学研究

目的:以龙血素B为检测指标,研究龙血竭在体内药动学参数。方法:以龙血素B为对照品,采用HPLC法测定两组家兔血浆中龙血素B的浓度。同时选择3P97软件进行处理。结果:与市售的广西龙血竭粉末相比,自制龙血竭缓释滴丸的达峰时间明显推迟,且达峰浓度明显降低(P0.05);但两制剂的药时曲线下面积(AUC),总清除率(CL)以及迟滞时间(Lag time)无显著差异(P0.05),相对生物利用度F=AUCT·DR/(AUCR·DT)×100%=101.53%。自制的广西龙血竭缓释滴丸符合权重W=1/C2的单室模型分布,具有较好的缓释效果且吸收程度与普通广西龙血竭粉末等效。结论:建立了用高效液相色谱法测龙血竭缓释滴丸中龙血素B含量的方法,得出了以龙血素B含量为主的缓释滴丸在家兔体内的药动学参数,为进一步深入研究两者剂型的生物等效性和药效学考察提供了实验依据。     

HPLC测定龙血竭胶囊中龙血素B的含量

目的:建立龙血竭胶囊的含量测定方法.方法:运用高效液相色谱法(HPLC)对龙血竭胶囊中龙血素B含量进行测定.色谱柱:Kromasil C18柱(4.6mm×200mm,5 μ),填充剂为十八烷基硅烷键合硅胶.流动相:乙腈-冰醋酸(1→90)溶液(40:60);流速:1.0ml/min;检测波长:270nm;柱温;室温.结果:龙血素B的量在0.18～0.90 μg之间呈良好线性关系,平均加样回收率101.5%,RSD=0.76%.结论:所建立的方法可靠,可用于龙血竭胶囊的质量控制.     

龙血竭中龙血素A在大鼠体内的药动学研究

目的考察龙血竭中龙血素A在大鼠体内的药动学行为。方法按100 g大鼠给予龙血竭250 mg灌胃给药,测定不同时间点的血药浓度,并以3P97软件计算其药动学参数。结果龙血素A的Ka为（1.46±0.19）h-1,t1/2（Ka）为（0.48±0.06）h,t1/2（K）为（2.59±0.67）h,tmax为（2.00±0.55）h,Cmax为（1.13±0.24）μg.mL-1,AUC为（2.84±0.68）μg.h.mL-1。结论龙血素A在大鼠体内符合线性动力学一室开放模型,吸收较快。     

HPLC法测定龙血竭中龙血素B的含量

目的建立龙血竭中龙血素B的含量测定方法。方法采用超声提取,HPLC测定法,用C18柱,以乙腈-1%醋酸溶液为流动相梯度洗脱,检测波长:278nm。结果龙血素B在3.93μg/ml-78.06μg/ml范围内,呈良好线性(r=0.9998),加样回收率平均为97.8%,重复性RSD%为0.4%。结论采用超声提取HPLC法测定龙血竭中龙血素B方法可行。     

RP-HPLC测定龙血竭中新化合物剑叶龙血素C含量

【目的】建立龙血竭中新化合物-剑叶龙血素C的分离与测定含量方法。【方法】采用PhenomenexC18(250 mm×4.6 mm,5μm)色谱柱,甲醇-水(75:25,v/v)为流动相,流速为1 m/min,检测波长为295nm,柱温50℃;用外标法定量。【结果】剑叶龙血素C在27.25~327 ng范围有良好线性关系,r=0.9997,理论塔板数以剑叶龙血素C计为4500;方法回收率为96.90%,RSD为1.2%。【结论】本方法是测定龙血竭中剑叶龙血素含量的准确的定量方法。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.