首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 76 毫秒
1.
 【目的】探讨冠心病患者血循环妊娠相关血浆蛋白A(PAPP—A)水平与内皮功能的关系。【方法】64例冠心病患者,其中稳定型心绞痛组34例,急性冠脉综合征组30例,分别检测其相关内皮功能,包括肱动脉血流介导的血管舒张反应(FMD)和高敏C-反应蛋白(hs—CRP)、内皮素(ET)、一氧化氮(NO)水平,并用酶联免疫法检测血清PAPP—A。【结果】稳定型心绞痛组患者同急性冠脉综合征组相比PAPP—A[(6×10^-3±5×10^-1U/L与(19×10^-3±13×10^-3)U/L,P〈0.05]、hs-CRP[(0.5±0.3)mg/L与(3.6±2.2)mg/L,P〈0.01]、NO[(57±4)μmol/L与(45±5)μmol/L,P〈0.05]和FMD(6.0%±0.8%与3-3%±1.2%,P〈0.05)的差异有统计学意义。逐步选择多重回归分析,按α=0.10水准,发现PAPP—A与hs—CRP和FMD存在直线关系,Lnhs—CRP的偏相关系数为0.333(95%置信区间:0.138-0.527,P〈0.01),FMD的偏相关系数为-0.623(95%CI:-1.144—0.102,P〈0.051,其中常数项为5.570。高PAPP—A(〉11.094×10^-3U/L)患者hs—CRP明显增高[(5.3±4.2)mg/L与(1.3±0.6)mg/L,P〈0.01),FMD则降低(3.3%±2.4%与6.2%±3.6%,P〈0.05)。【结论】PAPP-A可作为间接评估冠心病患者血管内皮功能的指标之一。  相似文献   

2.
目的探讨扩张型心肌病(DCM)患者超敏C反应蛋白(hs—CRP)水平与心功能的关系以及临床意义。方法比较hs—CRP正常组与hs—CRP升高组间NYHA心功能分级、左心室射血分数(LVEF)、左心室舒张末内径(LVEDD)的差异。分析不同NYHA分级患者的hs—CRP水平,并对hs—CRP与LVEF、LVEDD进行相关性分析。结果hs—CRP正常组NYHA分级为2.06±0.90级,LVEF为(38.58±5.98)%,LVEDD为58.94±6.14mm,hs—CRP升高组NYHA分级为2.89±0.88级,LVEF为(33.46±6.55)%,LVEDD为66.87±9.34mm,两组相比差异有显著性(P〈0.05)。NYHAI级组hs—CRP为2.66±1.80mg/L,Ⅱ级组为5.02±3.27mg/L,II级组为5.25±3.18mg/L,IV级组为8.84±2.60mg/L,Ⅱ级组、Ⅲ级组hs—CRP水平明显高于I级组(P〈0.05),Ⅳ级组明显高于前3组(P〈0.001)。hs—CRP水平与LVEF呈负相关(r=-0.438,P〈0.00t),与LVEDD正相关(r=0.346,P〈0.01)。结论hs—CRP水平与DCM患者心功能改变相关,hs—CRP可作为DCM患者心功能恶化的预测指标。  相似文献   

3.
【目的】观察骨形态发生蛋白-4(BMP-4)对人卵巢组织体外培养中始基卵泡存活和生长发育的影响。【方法】34例卵巢组织切成2mm×2mm×1mm皮质片,每例标本的皮质片均随机分成3组:研究组(BMP-4组)、对照组和非培养组,培养15d后行组织学检查、雌孕激素测定和凋亡分析。【结果】培养结束后对照组和研究组始基卵泡比例分别为58%±35%和48%±40%,低于非培养组(91%±9%),P〈0.05;窦前卵泡比例分别为52%±40%和42%±35%,高于非培养组(9%±9%),P〈0.05。研究组间质细胞凋亡指数和培养液孕激素浓度分别为(0.16±0.08)μg/L和(3.6±2.0)μg/L,低于对照组,对照组分别为(0.41±0.16)μg/L和(6±4)μg/L(P〈0.05)。【结论】BMP-4可以促进始基卵泡向窦前卵泡转化,降低细胞凋亡率,抑制孕激素分泌,是卵泡生长发育过程中的促进因子。  相似文献   

4.
Wang PH  Yu DM  Chu YJ  Song ZQ  Wang JC  Xu J  Ding M  Tian YF  Song D 《中华医学杂志》2007,87(26):1828-1831
目的回顾性分析249例糖尿病足(DF)深部感染病人的临床特点,并对影响伤口愈合的因素进行相关分析。方法根据病人是否截肢和伤口愈合情况,将249例DF深部感染病人分为未截肢愈合组(A组),截肢后愈合组(B组),截肢后未愈合组(c组),对其临床资料、实验室参数及足部伤口的特征进行比较和分析。结果A组病人为107例,B组病人为114例,C组病人为28例。A组病人年龄低于B组,B组年龄低于c组(59±12)岁、(67±11)岁,(72±9)岁。(P〈0.01),超敏C反应蛋白(hs—CRP)分别为A组:(18±5)mg/L,B组(13±5)mg/L,C组(7±6)mg/L(P〈0.01),A组血浆白蛋白高于B、C组分别为(32±7)g/L、(29±5)g/L,(28±3)g,(P〈0.01)。B组比A组病人糖尿病病程更长分别为(17±11)年、(10±6)年(P〈0.05),B组比A组病人更常见可探及骨质和存在脓性分泌物、坏死、骨暴露及恶臭味、水肿(P〈0.01或P〈0.05)。B组体温[(38.1±1.1)℃,(P〈0.05)],血白细胞计数[(10±3)×10^9/L,P〈0.05)],hs—CRP[(13±5)mg/L,P〈0.05)]均高于C组[(37.4±0.8)℃、(8±2)×10^9/L、(7±6)mg/L]。严重下肢缺血在A、B、c组分别为7%、37%、77%,差异有统计学意义(P〈0.05)。多因素Logistic回归分析,hs-CRP、血白蛋白为创面愈合的保护因素,高龄、脓性分泌物、可探及骨质、骨暴露、水肿、恶臭味、坏死、下肢严重缺血为创面愈合的危险因素。结论所有糖尿病足深部感染病人都需要外科的治疗,即使接受一个糖尿病足多学科团队的综合治疗,仍有部分的病人需要截肢。  相似文献   

5.
目的探讨血清脂蛋白(a)[Lp(a)]、纤维蛋白原(Fig)和超敏C反应蛋白(hs—CRP)与冠状动脉粥样硬化程度的关系,评价三者对冠心病的临床诊断价值。方法对358例疑诊冠心病而接受冠状动脉造影的患者同期检测血清Lp(a)、Fig和hs—CRP,并检测三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL—C)、低密度脂蛋白胆固醇(LDL—C)、尿素氮(BUN)和肌酐(Cr)。根据冠状动脉狭窄情况分为冠状动脉正常组(冠状动脉无狭窄,29例)、冠状动脉粥样硬化组(冠状动脉狭窄〈50%,91例)、冠状动脉单支病变组(仅有一支冠状动脉分支狭窄≥50%,103例)和多支病变组(有一支以上冠状动脉分支狭窄1〉50%,135例),并在每组内分高LDL—C亚组和正常LDL—C亚组。结果各组间Lp(a)和Fig水平依次递增,多支病变组和单支病变组较正常组分别升高了40.0%、22.3%(P〈0.05)和43.8%、25.5%(P〈0.05);且多支病变组与单支病变组比较差异亦有统计学意义(P〈0.05)。hs—CRP水平多支病变组[(0.94±0.83)mg/L]和单支病变组[(0.62±0.77)mg/L]与正常组[(0.16±0.43)mg/L]间差异均有统计学意义(P〈0.05);同时,多支病变组较单支病变组升高了50.9%(P〈0.05)。在剔除了LDL—C影响后亚组分析中,也得出类似结果。单因素Logistic回归分析表明血清Lp(a)、Fig和hs—CRP均为影响冠状动脉病变的因素,其中hs—CRP的OR值最大,OR为1.112,OR的95%CI(1.022—1.188)。进一步多因素Logistic回归分析表明,Lp(a)、Fig、hs—CRP、LDL—C和血糖均入选回归模型。hs—CRP与Lp(a)、Fig均呈正相关(r值分别为1.136和1.127,P〈0.01)。结论(1)随着冠状动脉病变程度加重,Lp(a)、Fig和hs—CRP水平显著增高。(2)hs—CRP对冠心病的预测价值大于Lp(a)和Fig,但Lp(a)和Fig与hs—CRP均呈正相关性。  相似文献   

6.
目的:观察血糖升高对老年急性心肌梗死(AMI)患者临床和预后的影响。方法:选取老年AMI患者223例,根据入院第一次随机血糖分为3组,A组〈7.8mmol/L,B组7.8~11.0mmol/L,C组≥11.0mmol/L。A组为血糖正常组,B、C组为血糖升高组。结果:入院随机血糖高的患者,平均年龄较大[(68.8±7.5)岁比(71.6±7.3)岁、(74.3±7.1)岁,P〈0.05],C组与A组相比血甘油三酯[(1.57±0.83)mmol/L比(1.31±0.63)mmol/L,P〈0.051、尿酸[(360.2±172.3)μmmol/L比(273±86.3)μmmol/L,P〈0.05]、肌酐[(125.2±90.5)μmmol/L比(85.7±23.6)μmmol/L,P〈0.05]浓度较高。与A组比较,B、C组复合壁心肌梗死多见(38.9%比59.7%、66.7%,P〈0.05)。C组与A组相比左室射血分数[(43.5±6.37)%比(58.5±8.36)%,P〈0.05]较低。与A组比较,B、c组患者溶栓再通率低(54.8%比40.6%、28.6%,P〈0.05),冠脉造影提示多支血管病变多见(51.6%比73.7%、69.2%,P〈0.05),C组比A组并发心力衰竭多见(35.4%比15.9%,P〈0.05),住院病死率(20.8%比4.5%.P〈0.05)增加。结论:老年A加患者入院随机帆糖升高.提示病情蘑、梗死面积大、并发症多、预后蓁及病死率高。  相似文献   

7.
刘荣静  习浩 《循证医学》2009,9(5):309-313
目的通过对不同血清标记物组合早期诊断急性冠脉综合征的价值进行比较,探讨妊娠相关血浆蛋白-A(PAPP-A)在提高组合诊断效能方面的作用。方法检测急性冠脉综合征患者、急性冠脉综合征疑似患者PAPP—A、超敏C反应蛋白(hs—CRP)和肌钙蛋白I(cTnI)水平,绘制3种标记物用于诊断急性冠脉综合征的受试者特征曲线(ROC曲线),再根据ROC曲线确定三者诊断急性冠脉综合征的诊断阈值,并对确立的标记物组合“PAPP—A+cTnI”、“cTnI+hs—CRP”和“PAPP—A+cTnI+hs—CRP”的诊断准确度进行比较。结果三种标记物诊断急性冠脉综合征的ROC曲线下面积分别为:PAPP—A0.978(95%C10.956~1.000),cTnI 0.873(95%C10.797—0.948),hs—CRP 0.841(95%C10.761~0.920);依此确定的三种标记物用于诊断急性冠脉综合征的诊断阈值为:PAPP.A17.225μg/mL,hs—CRP6.215mg/L,cTnI 1.030ng/mL。PAPP—A、hs—CRP、cTnI、“PAPP—A+cTnI”、“cTnI+hs—CRP”和“PAPP—A+cTnI+hs。CRP”的敏感度依次为0.889、0.733、0.689、0.956、0.956和0.956;特异度为0.956、0.778、1.000、0.956、0.778和0.756;诊断正确率为0.922、0.778、0.833、0.956、0.878和0.856。“PAPP—A+cTnI”较之于单独的标记物及其它组合,具有较高的诊断正确率(0.956)、较高的诊断特异度(0.956)、诊断敏感度(0.956)和阳性预测值(0.956)。结论组合“PAPP—A+cTnI”对急性冠脉综合征的综合诊断价值较高,对于急性冠脉综合征的早期诊断具有更高的诊断价值。  相似文献   

8.
目的探讨帕罗西汀对应激抑郁模型大鼠脑区蛋白激酶PKA、PKC和CaMKⅡ活力的影响。方法将成年雄性SD大鼠随机分为6组:对照组(Ⅰ)、抑郁模型组(Ⅱ)、抑郁模型+给药1次组(Ⅲ)、抑郁模型+给药1周组(Ⅳ)、抑郁模型+给药2周组(Ⅴ)和抑郁模型+给药4周组(Ⅵ)。抑郁模型为强迫大鼠游泳4周。采用同位素法检测蛋白激酶PKA、PKC和CaMKⅡ的活力。结果(1)在海马,Ⅱ、Ⅲ、Ⅳ及Ⅴ组大鼠PKA[分别为(3.92±0.23)×10^-2(3.68±0.092)×10^-2,(3.56±0.11)10^-2,和(3.52±0.18)10^-2]和CaMKⅡ[分别为(12.89±0.31)10^-2,(15.08±2.07)10^-2,(16.32±2.87)10^-2,和(17.00±1.52)10^-2】活力明显低于Ⅰ组[PKA(5.63±0.41)10^-2;CaMKII(48.91±1.86)10^-2]和Ⅵ组『PKA(4.92±0.36)10^-2;CaMKII(46.74±1.34)10^-2(P〈0.01或P〈0.05);Ⅱ组大鼠PKC的活力[(0.55±0.017)×10^-2明显低于对照组[(1.48±0.27)10^-2(P〈0.01),各用药组大鼠海马PKC活力与对照组比较差异无统计学意义(P〉0.05)(2)在前额叶皮质,Ⅱ、Ⅲ、Ⅳ组大鼠PKA活力[分别为(0.9±0.027)10^-2(0.92±0.081)10^-2(0.92±0.028)10^-2与对照组[(0.99±0.072)10^-2]比较差异无统计学意义(P〉0.05);而Ⅴ【(1.14±0.045)10^-2]和Ⅵ[(1.27±0.040)10^-2]组的PKA活性则显著高于其它四组(P〈0.01);Ⅱ和Ⅲ组的PKC活性[分别为(0.15±0.013)10^-2(0.14±0.007)10^-2)]均显著高于对照组[(0.099±0.0007)10^-2]和其它用药组(P〈0.01),Ⅳ组PKC活性【(0.11±0.0006)10^-2】与Ⅰ组比较差异无统计学意义(P〉0.05),Ⅴ和Ⅵ组PKC活性[分别为(0.077±0.0005)10^-2,(0.03±0.00017)10^-2]显著低于Ⅰ组(P〈0.01);模型组[(6.84±0.22)10^-2]和各用药组[分别为(6.68±0.23)10^-2,(6.89±0.15)×10^-2(6.55±0.14)10^-2,(6.53±0.13)10^-2]的CaMKII活性显著低于对照组[(16.57±0.19)10^-2(P〈0.01)。结论帕罗西汀长期用药逆转慢性应激所致大鼠海马PKA、PKC和CaMKⅡ活力降低,而对前额叶皮质PKA、PKC和CaMKⅡ活力改变的作用复杂。  相似文献   

9.
慢性乙肝患者拉米夫定耐药后出现肝功能衰竭的危险因素   总被引:1,自引:0,他引:1  
【目的】探讨慢性乙型肝炎患者拉米夫定耐药后出现肝功能衰竭的危险因素。【方法】拉米夫定耐药后导致病情加重的慢性乙型肝炎患者56例纳入研究.记录拉米夫定治疗前病程、诊断、拉米夫定疗程,检测并记录临床耐药时的肝功能、乙肝两对半、HBVDNA定量、YMDD变异及前C区变异。按病情加重后的诊断分为肝功能衰竭组和慢性乙型肝炎组进行比较。【结果】肝功能衰竭组患者年龄(45±13)岁,大于慢性乙型肝炎组(37±131岁(P〈0.05);肝功能衰竭组临床耐药时HBV DNA载量高于慢性乙型肝炎组[(2.8×10^8+4.9×10^8)拷贝/mL vs(3.1×10^6±2.9×10^6)拷贝/mL;P〈0.05];肝功能衰竭组出现HBeAg/Anti—HBe血清学转换(54.6%)高于慢性乙型肝炎组(18.4%,P〈0.05)。年龄和拉米夫定治疗前诊断为肝硬化都是耐药后出现肝功能衰竭的独立危险因素。【结论】拉米夫定治疗前年龄大、诊断为肝硬化,临床耐药时病毒载量高,耐药后出现HBeAg/Anti—HBe血清学转换可能是拉米夫定治疗耐药后出现肝功能衰竭的危险因素。有肝硬化基础患者一旦发生耐药变异,应及时使用能治疗耐药变异的阿德福韦或恩替卡韦。  相似文献   

10.
Zeng SG  Liu JC  He M  Huang QR 《中华医学杂志》2007,87(23):1637-1640
目的 探讨油茶皂苷C(CS—c)预处理对培养乳鼠心肌细胞缺氧/复氧(A/R)损伤的保护作用及作用机制。方法 采用培养3—4d的SD大鼠心肌细胞,随机分为5组(每组8孔):正常对照组,单纯缺影复氧组(A/R),缺氧预适应组(AP),油茶皂苷C预处理(CS—C),格列苯脲预处理组(Glib)。采用心肌细胞缺氧/复氧损伤模型,各组于复氧1h时进行以下指标检测:(1)心肌细胞存活率;(2)培养液中乳酸脱氢酶(LDH)含量;(3)透射电镜观察心肌细胞超微结构改变。结果 与对照组比较,A/R组LDH含量(57.8U/L±6.4U/L)高于对照组(12.3U/L±1.7U/L),差异有统计学意义(P〈0.01),心肌细胞存活率(51.0%±1.9%)低于对照组(92.0%±2.0%),差异有统计学意义(P〈0.01),细胞超微结构损害明显;CS—C预处理组LDH含量(39.8U/L±3.9U/L)、心肌细胞存活率(76.4%±3.5%)低于A/R组(P〈0.01),其结果与AP组(分别为32.4U/L±5.2U/L,78.0%±2.0%,P〉0.05)相似,Glib组LDH含量(55.8U/L±5.0U/L)高于CS—C预处理组、心肌细胞存活率(54.1%±3.7%)低于CS-C预处理组(P〈0.05)。结论 CS—C预处理抗乳鼠心肌细胞缺氧/复氧损伤作用与缺氧预适应作用相似,其保护作用可能与KATP^+,通道开放有关。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号