抗人大肠癌免疫磁性白蛋白微球的抗癌效果

目的:观察自制丝裂霉素C免疫磁性白蛋白微球(MMC-IMAMS)体外及体内抗大肠癌效果。方法:将丝裂霉素C免疫磁性白蛋白微球(MMC-IMAMS)与丝裂霉素C磁性白蛋白微球(MMC-MAMS)、单纯丝裂霉素C(MMC)比较,MTT法分别检测其体外对大肠癌细胞株LoVo的杀伤作用,然后通过移植人大肠癌裸鼠模型检测体内的肿瘤抑制率。结果:体外实验丝裂霉素免疫磁性白蛋白微球较单纯丝裂霉素磁性白蛋白微球有更强的肿瘤细胞抑制效率;裸鼠模型体内实验丝裂霉素免疫磁性白蛋白微球组肿瘤抑制率较丝裂霉素磁性白蛋白微球组和单纯丝裂霉素组具有显著差异。且丝裂霉素免疫磁性白蛋白微球是安全的,在体外磁场作用下可较长时间内沉积于靶区。结论:丝裂霉素免疫磁性白蛋白微球安全性好,靶向性更强大,体内体外均有更强的抗癌效果。     

抗癌药阿霉素磁性蛋白微球的制备及其细胞毒试验

利用加热固化蛋白质原理制备出阿霉素磁性蛋白微球，比色法分析其细胞毒作用。结果显示阿霉素磁性蛋白微球具有磁性药物微球的形态学特征， 体外抑制肿瘤细胞生长的能力与阿霉素相似 （Ｐ＞ ０．０５）， 提示阿霉素磁性蛋白微球是抗癌药物阿霉素的一种新剂型，该药物剂型的改变未影响其体外药理作用，与磁场联用发挥靶向高效低毒的抗癌作用     

5-氟尿嘧啶磁性白蛋白微球对胰腺磁靶向性实验研究

目的探讨磁导向下5-氟尿嘧啶磁性白蛋白微球对胰腺的磁靶向性。方法取Wistar大鼠12只,分为两组,一组为5-Fu组,另一组为5-氟尿嘧啶磁性白蛋白微球组;分别经供胰动脉注入5-Fu和5-氟尿嘧啶磁性白蛋白微球(按5-Fu 8mg/kg),在该组的大鼠胰腺表面加一表面强度为4250GS的磁场,30min后,取其胰腺。用HPLC法检测其药物浓度。结果5-氟尿嘧啶磁性白蛋白微球组胰腺中的药物浓度(57.95±6.39μg/ml)是5-Fu组(16.58±3.19μg/ml)的3.50倍,明显高于5-Fu组(P<0.01)。结论磁性白蛋白微球作为一种新的药物载体经供胰动脉注入后在磁导向下对胰腺具有良好的磁靶向性。     

As2O3磁性Fe3O4白蛋白微球的制备与表征

目的 :制备用于肿瘤热化疗和逆转多药耐药的As2O3磁性Fe3O4白蛋白微球并表征。方法:化学共沉淀法制备Fe3O4磁性纳米粒,运用透射电镜、X射线衍射分析进行表征,溶血实验及MTT试验进行毒理学评定。去溶剂化交联法制备As2O3磁性Fe3O4白蛋白微球,运用透射电镜和能谱仪进行表征,在交变磁场作用下进行体外升温试验,体外释药方法研究其释药速率。结果:Fe3O4磁性纳米粒近似球形,粒径约20 nm,无溶血作用,细胞毒性为1级。As2O3磁性Fe3O4白蛋白微球近似球形,大小均匀,粒径约193.4 nm,其不同浓度的磁流体在交变磁场下可升温至39.5~58.0℃并保持恒定。体外释药实验证实As2O3磁性Fe3O4白蛋白微球具有明显的缓释功能。结论:Fe3O4磁性纳米粒作为药物载体具有良好的生物相容性。用去溶剂化交联法可以成功制备出As2O3磁性Fe3O4白蛋白微球,As2O3磁性Fe3O4白蛋白微球释药速率缓慢,为研究肿瘤热化疗和逆转多药耐药提供理论和实验基础。     

纳米磁性靶向药囊对人肝癌裸鼠移植瘤bcl-2/bax蛋白表达的影响

目的观察纳米磁性5-Fu药囊靶向治疗人肝癌裸鼠移植瘤对肿瘤细胞凋亡bcl-2及bax蛋白表达的影响。方法透射电镜观察各组移植瘤形态学特征，免疫组化SABC法测定bcl-2和bax蛋白表达水平。结果电镜观察可见B组（5-Fu）和D组（纳米磁性5-Fu药囊加内磁场）肿瘤组织大量癌细胞凋亡。B和D组bcl-2蛋白表达水平低于A组（生理盐水对照）、C组（纳米磁性5-Fu药囊）和E组（纳米磁小体加内磁场）（P〈0．01），D组bcl-2蛋白表达水平低于B组（P〈0．01）；B组和D组bax蛋白表达水平高于其他三组（P〈0．01），D组bax蛋白表达水平高于B组（P〈0．01）。结论纳米磁性5-Fu药囊靶向治疗能够降低bcl-2蛋白表达，增加bax蛋白表达，促进人肝癌裸鼠移植瘤细胞凋亡，提高5-Fu抗肿瘤作用。     

阿司匹林磁性壳聚糖微球的制备及其性能

目的 以阿司匹林为药物模型,制备阿司匹林磁性壳聚糖微球(AMCM),研究其体外释放度与体内靶向性.方法 利用壳聚糖为骨架材料,以Fe3O4作为磁性内核,戊二醛作为交联剂,固载阿司匹林,研制AMCM,采用动态透析法及分光光度法进行体外释药实验;设定纯种兔为一靶区,外加磁场,经兔耳缘静脉注射AMCM,观察AMCM在体内分布情况,并观察外加磁场强度对微球在靶区定位的影响.结果 制备的AMCM成球性能好,无粘连,粒径1～8 μm,载药率0.922(W/W),包封率82.4%,具有显著的缓释阿司匹林作用,经外加磁场下的AMCM有较好的靶向分布性且与磁场强度相平行.结论 自制的AMCM具有显著的体外缓释与体内靶向作用.     

平阳霉素磁性微球的制备及特性检测

目的 :制备平阳霉素磁性微球 ,对其特性进行检测并评价其靶向性。方法 :采用冷冻凝聚法制备包裹超微Fe3O4 和平阳霉素的明胶微球 ,测定微球的直径 ;应用液相高效色谱仪、原子吸收分光光度仪、药物溶出仪等测定微球各成分含量、释放率及在外加磁场作用下微球在大鼠体内的分布情况。结果 :微球呈球形 ,平均直径 32 .2 3μm各成分含量为 (W/W ) :Fe3O4 11.7%,PYM3 .8%,明胶 84.5 %。 3h内平阳霉素释放率 6 3 .1%。Fe3O4 在大鼠靶向部位分布明显高于非靶向部位。结论 :平阳霉素磁性微球具有较好的靶向性和缓释性 ,是治疗海绵状血管瘤较为理想的剂型。     

平阳霉素磁性微球的制备及特性检测

目的：制备平阳霉素磁性微球，对其特性进行检测并评价其靶向性。方法：采用冷冻凝聚法制备包裹超微Fe203和平阳霉素的明胶微球，测定微球的直径；应用液相高效色谱仪、原于吸收分光光度仪、药物溶出仪等到定微球各成分含量、释放率及在外加磁场作用下微球在大鼠体内的分布情况。结果：微球呈球形，平均直径32．23μm各成分含量为(W／W)：Fe30411．7％，PYM3．8％，明胶84．5％。3h内平阳霉素释放率63．1％。Fe3O4在大鼠靶向部位分布明显高于非靶向部位。结论：平阳霉素磁性微球具有较好的靶向性和缓释性，是治疗海绵状血管瘤较为理想的剂型。     

抗癌药阿霉素磁性蛋白微球的制备及其细胞毒试验

利用加热固化蛋白质原理制备出阿霉素磁性蛋白微球,比色法分析其毒作用。结果显示阿霉素磁性蛋白微球肯有磁性药物微球的形态学特征,体外抑制肿瘤细胞生长的能力与阿霉素相似（Ｐ〈０．０５）,提示阿霉素磁性蛋白微球是抗癌药物阿霉素的一种新剂型,该药物剂型的改变未影响其体外药理作用,与磁声联用发挥靶向高效低毒的抗癌作用。     

2种聚乙二醇不饱和酸单酯共聚合包被Fe3O4制备磁性微球及其表征

目的：用两种聚乙二醇（Polyethylene glycol,PEG）不饱和酸单酯经自由基共聚包被纳米Fe3O4磁芯制备磁性微球,考察其功能化后特异性和非特异性结合作用。方法：水解沉淀法制备纳米Fe3O4磁芯;用PEG-350单甲醚丙烯酸酯和PEG-1540单顺丁烯酸单酯为单体,在正庚烷-水组成的油包水体系自由基共聚制备磁性微球;将微球表面来自PEG-1540的羟基经甲磺酸酯活化、连接乙二胺后再用活化生物素修饰。透射电镜观察其外貌;测定与碱性磷酸酶标记链亲和素（Streptavidin labeled by alkaline phosphate,AP-SAV）的特异性结合、与4-硝基-1-萘酚苯甲酸酯等的非特异性结合。结果：所得Fe3O4粒径60 nm左右,生物素修饰后粒径接近200 nm。此磁性微球可从混合蛋白中选择性分离碱性磷酸酶酶标记的链亲和素,结合容量约51 ng蛋白/g磁性微球;4-硝基-1-萘酚苯甲酸酯在浓度很低时同此磁性微球的结合低于检测限,在浓度高达64 ?滋mol/L时仍无显著结合。结论：用两种PEG不饱和酸酯共聚包被Fe3O4可制备表面亲水磁性微球,功能化后有特异相互作用且非特异性作用很弱,但还需提高此磁性微球功能化后的特异性结合容量。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.