HPLC法测定白花蛇舌草中槲皮素和山奈素的含量

目的:建立高效液相色谱法测定白花蛇舌草中槲皮素和山奈素含量的方法.方法:采用伊利特-C18柱(4.6mm×250mm 5μm),流动相为乙腈-甲醇-0.2%磷酸(体积比10∶45∶60),流速为1 ml/min,检测波长为370 nm.结果:槲皮素在4.20～25.20μg(r=0.9999),山奈素在0.97～11.64 μg(r=0.9997) 的范围内与峰面积呈良好线性关系.平均加样回收率分别为100.48%,97.85%,RSD分别为1.01%(n=3),1.36%(n＝3).结论:本法快速、简便、准确,可用于分别测定白花蛇舌草中槲皮素和山奈素的含量,为评价白花蛇舌草的内在质量提供科学依据.     

RP-HPLC法测定仙鹤草药材中槲皮素的含量

目的 建立反相高效液相色谱法测定仙鹤草药材中槲皮素含量的方法.方法 采用Phenomenex Gemini-C18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-甲醇-0.2%磷酸(体积比10∶45∶60),流速为l ml/min,检测波长为368 nm,柱温30 ℃.结果 槲皮素在0.098～1.746 μg范围内线性良好(r=0.9999);平均加样回收率(n=6)为99.9%,RSD为1.4%;不同批号的仙鹤草药材中槲皮素的含量为10.6～12.5 μg/g.结论 本方法简便、准确,重复性好,适用于仙鹤草药材的质量控制.     

炎宁颗粒质量标准提高研究

目的:提高炎宁颗粒的质量标准。方法:采用TLC法对制剂中的白花蛇舌草进行定性鉴别;用RPHPLC法对制剂中的槲皮素和木犀草素进行含量测定。色谱柱:AgilentHCC18(250mm×4.6mm,5μm);流动相:甲醇∶0.4%磷酸水溶液(48∶52);检测波长:350nm。结果:TLC法能对白花蛇舌草进行专属性定性分析;槲皮素和木犀草素分别在0.141~0.705μg(r=0.9994)和0.101~0.505μg(r=0.9995)范围内线性关系良好,槲皮素和木犀草素的加样回收率分别是98.51%(RSD为1.82%)和98.30%(RSD为1.40%)。结论:本文所建立的质量控制方法可准确地进行定性和定量检测,方法可靠,重复性好,对进一步完善炎宁颗粒的内在质量控制标准具有重要的参考价值。     

HPLC法测定白花蛇舌草注射液中去乙酰基车叶草苷酸和鸡屎藤次苷

目的:建立白花蛇舌草注射液中去乙酰基车叶草苷酸和鸡屎藤次苷的测定方法。方法: 采用HPLC法测定,色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-乙腈-0.1%冰醋酸(3∶2∶95),检测波长230 nm,体积流量1.0 mL/min。结果:去乙酰基车叶草苷酸和鸡屎藤次苷的浓度分别在7.50～90.0 μg/mL(r=0.999 7)和6.00～72.0 μg/mL(r=0.999 9)线性关系良好;平均加样回收率分别为98.1%、99.0%,RSD分别为1.2%、1.1%。结论:本法快速、简便、准确, 可用于同时测定白花蛇舌草注射液中上述两种环烯醚萜类成分。     

反相高效液相色谱法测定人字草中槲皮素和木犀草素的含量

目的建立人字草中槲皮素和木犀草素含量测定方法。方法采用反相高效液相色谱(RP-HPLC)法,色谱柱为Zorbax SB-C18柱(4.6mm×250mm,5μm);检测波长360nm,流动相为甲醇-0.2%磷酸(50∶50),柱温30℃,流速1.0mL/min。结果槲皮素、木犀草素的回归方程分别为Y=2545.36X+86.37,Y=3261.65X+61.29;r分别为0.9995和0.9991;质量浓度线性范围分别是5.28-26.4μg/mL,4.12-20.6μg/mL;槲皮素、木犀草素的加样回收率分别为101.5%和99.6%,RSD分别为1.2%和0.83%;样品分别含槲皮素、木犀草素2.33mg/g,0.316mg/g。结论该方法适合同时测定人字草中槲皮素和木犀草素的含量,方法简便可行,重复性好,结果可靠。     

HPLC法测定不同产地白花蛇舌草中熊果酸的含量

目的:建立白花蛇舌草中熊果酸的含量测定方法,并检测不同产地白花蛇舌草中熊果酸的含量。方法:采用高效液相色谱法测定。色谱柱为Alltech RP-C18(4.6 mm×250 mm,5μm);流动相为甲醇-水(90∶10);检测波长是210nm;流速为1.0 mL/min;柱温为30℃。结果:熊果酸的线性范围在10.916～152.824μg/mL之间,r=0.9997,线性关系良好。平均回收率为98.48%,RSD为1.27%。结论:本方法简便、准确、重现性好,可作为该药材的质量控制方法。     

高效液相色谱法同时测定心迪软胶囊中3种黄酮苷元的含量

目的建立心迪软胶囊的质量标准.方法采用高效液相色谱法对心迪软胶囊中的槲皮素、山柰素和异鼠李素同时进行含量测定: Diamonsil (钻石) C18 (250 mm×4.6 mm,5 μm),乙腈-水-磷酸(30∶70∶0.1)为流动相,流速1 mL/min,检测波长370 nm,柱温40℃.结果槲皮素在0.4118～1.6475 μg的范围内线性关系良好,r=0.9998, 平均回收率为96.81%,RSD为0.92%(n=6);山柰素在0.0208～0.0832 μg的范围内线性关系良好,r=0.9998,平均回收率为96.92%,RSD为2.02%(n=6);异鼠李素在0.1829～0.7316 μg的范围内线性关系良好,r=0.9999,平均回收率为97.06%,RSD为1.63%(n=6).结论高效液相色谱法简便、灵敏、准确,能有效地控制心迪软胶囊的质量.     

HPLC法测定苦参药材中苦参酮、降苦参酮的含量

目的 建立测定苦参药材中 2种黄酮类成分苦参酮和降苦参酮含量的高效液相色谱方法.方法 采用 Phenomenex Gemini C18色谱柱 ( 250 mm×4.6 mm,5 μm ),以乙腈-水 ( 50∶ 50 )为流动相,流速 1.0 mL /min,检测波长 290 nm.结果 苦参酮和降苦参酮的线性范围分别为 0.318 6～1.593 0 μg (r=0.999 8),0.112 8～0.564 0 μg (r=0.999 9 );苦参药材平均加样回收率 (n=6) 分别为100.56% (RSD=0.93%),96.66%(RSD=0.85%).结论 3批样品测定结果表明,该方法简便、准确、重现性良好,可用于苦参药材苦参酮和降苦参酮 2种黄酮类成分的含量测定.     

薄层扫描法测定三种中药材中熊果酸的含量

采用薄层扫描法对枇杷叶、白花蛇舌草、女贞子中熊果酸的含量进行了测定,其薄层扫描测定条件为:展开剂中环己烷-氯仿-乙酸乙酯(20∶5∶8);显色剂用10%H2SO4乙酸溶液。结果表明:在三种药材中,白花蛇舌草熊果酸含量最高,达0.896%;枇杷叶稍低,为0.843%;女贞子最低,为0.436%。该测定方法简便、快速,测定结果准确、可靠。     

RP-HPLC法测定槐米中槲皮素的含量

目的　建立 RP- HPL C法测定槐米中槲皮素的含量。方法　使用 Shim- pack CL C- ODS柱 (6 .0 m m× 15 0mm,5 μm) ,以甲醇 - 0 .4 %磷酸溶液 (7∶ 3)为流动相 ,375 nm波长 ,测定槐米水解液中槲皮素的含量。结果　该方法的线性为 10 .0 3～ 10 0 .3ng,r=0 .9997;平均回收率为 99.0 6 % ,RSD=2 .0 7% (n=9)。结论　该方法测定样品分离效果佳 ,灵敏度高 ,重现性好 ,结果准确可靠。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.