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1.
Li M  You S  Ge W  Ma S  Ma N  Zhao C 《中华医学杂志(英文版)》2003,116(11):1655-1661
Objectives To assess the feasibility and efficacy of eliciting leukemia-specific T-cell responses in syngeneic mice in vitro and in vivo using dendritic cells (DCs) pulsed with total RNA from leukemia cells.Methods DCs generated from bone marrow culture in vitro in the presence of combined cytokines were pulsed with cellular total RNA isolated from cultured L615 cells by cationic lipid 1,2-dioleoyloxy-3-(trimethylammonium) propane (DOTAP). T-cell responses were evaluated by in vitro proliferation, and cytotoxicity assay. And in vivo immune protection and proghosis of mice with leukemia were studied.Results DCs pulsed with total RNA isolated from cultured L615 cells (DCs/RNA) were remarkably effective in stimulating L615-specific T-cell response in vitro, but did not cross-react with other leukemia cells from syngeneic mice. Vaccination of naive mice with viable DCs/RNA vaccine was able to partly protect from challenge with a lethal dose of live L615 cells, leading to low leukemia incidence and overall survival prolongation. Statistically significant survival was also observed in a low lethal dose of L615-bearing mice that received treatment using viable DCs∕RNA vaccine alone, suggesting that systemic administration of IL-2 could enhance the anti-tumor efficacy of leukemia RNA/DCs vaccine.Conclusions These data support the use of DCs/RNA vaccine as a feasible and effective route to elicit leukemia immunity against unidentified leukemia-associated antigens for treatment of leukemia-bearing animals.  相似文献   

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Objective To observe the immunotherapeutic effects of dendritic cells vaccine pulsed with tumor cell lystate on mice with pancreatic carcinoma. Methods Dendritic cells (MTSC4) were pulsed with tumor cells lysate. The immune preventative and immnotherapeutic effects of DC vaccines on mice with pancreatic carcinoma were assessed. Results After vaccination of the DC vaccines,mice remained tumor-free for at least 25 days in DCs vaccines group,but in other groups the subcutaneous implantation tumorigenesis were found beginning 3 to 9 days. CTL stimulated by DC vaccines effected cytolytic activity against pancreatic carcinoma cells. The survival period was obviously prolonged in DCs vaccines group (56 ±9)d than in other groups P<0.01) and tumors (1.4 ±0.8)g in DCs vaccines group were significantly smaller than that in other groups (P < 0. 05). Conclusion Tumor cell lysate-pulsed dendrtic cells vaccines can induce a specific and effective immune response against pancreatic carcinoma cell implanted in mice. 4  相似文献   

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This study examined the effects of a recombinant adenovirus AdTEN-EGFP on the proliferation of A549 cells, a human lung carcinoma cell line, in vitro and on the growth of the implanted tumors in the nude mice in vivo, explored the underlying mechanisms and evaluated the in vitro transfection efficiency of Ad-PTEN-EGFP into A549 cells. The expression of Ad-PTEN-EGFP in the A549 cells was determined. The proliferation and the apoptosis rates of the A549 cells with Ad-PTEN-EGFP transfection or not was detected by MTT and flow cytometry. Ad-PTEN-EGFP at different doses was injected intratumorally to the tumor-bearing mice induced by the A549 cells. Tumor sizes were measured on an alternate day. After all the mice were sacrificed, the implanted tumors were removed for routine histological examination, weight test, HE staining and immunohistochemical staining. The expressions of Bax, P16 and P53 in the tumor tissues and those of caspase-3, CD34 and VEGF in the mouse sera were detected. Tumor cell apoptosis was measured by TUNEL method. The results showed that the vitality of the A549 cells after transfection with Ad-PTEN-EGFP declined. The expression of green fluorescent protein was observed under fluorescent microscope. The transfection rate was in excess of 50%. The mRNA and protein expression of PTEN in the transfected cells was confirmed. The proliferation rate of the transfected cells was significantly decreased when compared with that of the non-transfected cells (P〈0.05). The number of the apoptosi's cells was increased in the transfected cells (P〈0.05). The models of implanted tumors were successfully estab- lished by injection of the A549 cells in the flank of Balb/c nude mice. Administration of Ad-PTEN-EGFP to the tumor-bearing nude mice resulted in a suppression of tumor growth. There were statistically significant differences in the tumor weight and tumor volume between the Ad-PTEN-EGFP-treated group and the control groups (P〈0.05). In contrast to those in the control groups, tumor tissues in the Ad-PTEN-EGFP-treated group were shown to have typical extensive vacuolar degeneration and massive hemorrhagic necrosis. Apoptotic bodies were also observed in the tumor cells. The expressions of Bax, caspase-3 and P16 were increased (P〈0.05) while those of CD34, VEGF and P53 decreased (P〈0.05) in the Ad-PTEN-EGFP-treated group. It is concluded that Ad-PTEN-EGFP could induce the apoptosis of the A549 cells and inhibit their proliferation. And it could also substantially suppress the tumor growth in the tumor-bearing nude mice and induce apoptosis of the tumor cells as well. These findings carry significant implications for adenovirus vector-based PTEN gene therapies for lung cancers.  相似文献   

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Objective: To observe the effect of anciclovir (ACV) treatment on tumors induced by inoculation of TK gene-transfected human pulmonary adenocarcinoma A549 cells in nude mice. Methods: A recombinant plasmid containing TK gene was constructed and transfected into A549 cells by electroporation. The sensitivity of the transgenic cells (A549-TK) to ACV was examined by MTT assay in vitro and for in vivo observation, inoculation of A549-TK and A-549 cells into nude mice was separately performed to induce tumor growth, the response of which to ACV treatment was observed, and the tumor tissues were pathologically examined. Results: A recombinant plasmid containing TK gene was successfully constructed and transfected into A549 cells. The sensitivity of A549-TK cells to ACV was 43 times higher than that of A549 cells. The tumors induced by A549-TK cells showed no significant increase in size after ACV treatment (P>0. 05) , and light microscopy revealed local tissue necrosis, karyoklasis, and nuclei disappearance.  相似文献   

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To compare the difference in tumor immunity and autoimmunity elicited by adenovirus(Ad) encoding human or murine tyrosinase-related protein 2(AdhTRP2 or AdmTRP2),and to find the most effective way to induce immunity by AdhTRP2 or AdmTRP2,C57BL/6 mice were im-munized with AdhTRP2 or AdmTRP2 intramuscularly at different doses of 105,106,107 and 108 separately(10 mice for each dose).Two weeks after the immunization,in vivo CTL assay and in-tracellular staining(ICS) of IFN-γ were carried out to analyze the dose-effect relationship.Tumor growth and vitiligo(as an sign of autoimmunity) were observed until 3 months after challenge with 105 B16F10 tumor cells.The results showed that Ad encoding AdmTrp2 induced weak tumor im-mune response.Similar immunization with AdhTrp-2 elicited stronger protective immunity.CTL activity and IFN-γ-produced CD8 T cells were directly proportional to dose of AdhTrp2 or AdmTrp2.Moreover,AdhTrp2 group showed tumor rejection in 100% of challenged mice till the end of 3rd month while 60% of mice immunized with AdmTrp2 were protected against tumor.In the whole process of this experiment,no vitiligo was observed in mice immunized either with AdhTrp2 or AdmTrp2.It is concluded that anti-melanoma responses induced by genetic vaccina-tion expressing xenoantigens breaks immune tolerance effectively and is able to elicit strong anti-gen-specific cytotoxic T cell response without vitiligo.  相似文献   

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Summary: The specific anti-tumor immune response induced by mouse bone marrow dendritic cells (DCs) lransfected with recombinant adenovirus carrying mutant k-ras genes was investighted. DCs were generated from mouse bone marrow in the presence of rmGM-CSF (3.3 ng/mL) and rmIL-4 (1.3 ng/mL) and detected by FACS, and then transfecled with the recombinant adenovirus encoding mutant k ras gene. The efficacy of transfection and T cell stimulating activity of DCs were detected. CTL activity of the mice vaccinated with DCs was observed. The resuhs showed thai DCs had dendritic veiled morphology. BmDCs highly expressed B7-1(80%), B7-2(77%), MHC Ⅱ (70%), CDllc (65%), CD40 (70%) and CD54 (96%) with FACS, and no significant difference in the expression was observed before and after the transfection (P〈0.05). The DCs transfeeled by mutant k-ras gene could significantly stimulate lymphoeytes proliferation as compared with those transfeeted by Ad e or non-modified DCs (P〈0.05). DC vaccine transfected by mutant k-ras gene could induce CTL activity against Lewis lung cancer, but not against B16. The specific eytotoxicity against Lewis lung cancer in Ad-k-ras/12-transdueed DC group was signifieantly higher than those in the control, vector and non transfeeted DCs groups (P〈0.05). It was concluded that special antitumor response could be induced by DCs transfected with recombinant adenovirus carrying mutant k-ras genes.  相似文献   

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Objective To investigate the antitumor immune efficiency of mouse dendritic cells (mDCs) by using adenovirus-mediated tumor necrosis factor-alpha (AdV-TNF-α) gene transfer.Methods MDCs infected with AdV-TNF-α and AdV-pLpA (no gene insert) at 100 multiplicity of infection (MOI) were analyzed by RNase protection assay for their cytokine secretion. Mixed lymphocyte reactions were also performed to analyze their capacity for alloantigen-presentation. C57BL/6 mice were challenged with R3LL tumor cells (Lewis lung carcinoma line) 10 days after vaccination with different engineered DCs and regular DCs as well.Results Compared to AdV-pLpA and mock-infected DCs, AdV-TNF-α-infected DCs displayed up-regulated expression of alpha tumor necrosis factor, interleukin-12 (IL-12), interleukin-18 (IL-18) and granulocyte macrophage colony stimulation factor (GM-CSF), and indicated stronger allogeneic T cell proliferative responses. Furthermore, vaccination of mice with dendritic cell tumor necrosis factor-alpha (DCTNF-α) pulsed with Mut1 peptide induced more efficient tumor-specific cytotoxic T lymphocyte (CTL) cytotoxicity against R3LL tumor cells in vitro and with efficient antitumor immunity in vivo. Conclusion This type of engineered DCs could be applied in clinical settings of DC-based cancer vaccines  相似文献   

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Objective To study the effect of dendritic cells (DCs) transfected with sCD40-EGFP on T-cell proliferation and the cytotoxic T lymphocyte cytotoxic activity. Methods The expressing vector pEGFP-N1 /sCD40 was transfected into DCs via lipofectamine reagent mediation. Peripheral blood mononuclear cells (PBMCs) from Balb/c mice as reaction cells and DCs from sCD40 transfection group,  相似文献   

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Dendritic cells (DCs), as the most potent antigen presenting cells in vivo, are central to generating specific immune responses and can be used as important vectors in antitumour immunity. DCs loaded with tumour antigens can induce specific antitumour response in cytotoxic T lymphocytes (CTL) . P-glycoprotein ( P-gp), a product of mdrl gene, widely overexpressed in many multidrug resistant (MDR) tumour cells, is a main mechanism that makes tumour cells escape from death induced by chemotherapeutic agents.  相似文献   

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目的:建立表达EB病毒LMP2A小鼠移植瘤模型,为树突状细胞治疗EB病毒相关肿瘤的动物试验奠定基础。方法:将EB病毒LMP2A基因克隆至逆转录质粒pLXSN中,重组质粒用脂质体Clonfectin转入BALB/c小鼠来源的骨髓瘤细胞SP2/0,经G418筛选获得阳性克隆,亚克隆得到EB病毒LMP2A表达株并经PCR、RT—PCR、IFA、流式细胞仪鉴定。腹股沟皮下接种该细胞入BALB/c小鼠得到移植瘤模型,两周后切除肿瘤组织并作苏木精-伊红(H—E)染色切片。结果:构建出含EB病毒LMP2A基因的重组逆转录质粒,获得表达EB病毒LMP2A的SP2/0细胞,该细胞在BALB/c小鼠可生长成肿块。结论:成功地构建表达EB病毒潜伏膜蛋白2A(LMP2A)的小鼠移植瘤模型。  相似文献   

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目的 构建EB病毒(Epstein-Barr virus, EBV)EBNA1和LMP1融合基因的重组腺病毒,探究重组腺病毒的免疫学作用。方法 以质粒pCXWB-EBNA1和pMV261-LMP1为模板,通过PCR扩增EBNA1和LMP1基因,并通过linker以重叠延伸PCR的方式构建融合基因EBNA1-LMP1,将其与腺病毒穿梭质粒pDC316-mCMV-EGFP通过双酶切、连接构建重组质粒EBNA1-LMP1-pDC316-mCMV-EGFP,并通过PCR、酶切和基因测序进行鉴定。将重组质粒EBNA1-LMP1-pDC316-mCMV-EGFP与包装质粒pBHGlox(delta)E1,3Cre转染293T细胞以获得重组腺病毒;通过Western blot检测融合基因EBNA1-LMP1在293T细胞中的表达;用重组腺病毒免疫C57BL/6J小鼠,测定小鼠体内CD4+T细胞、CD8+T细胞和细胞因子TNF-α、IFN-γ的比例,观察重组腺病毒的免疫效果。结果 通过PCR获得基因EBNA1(717 bp)和LMP1(1 161 bp),通过重叠延伸PCR获得EBNA1-LMP1 (1 923 bp)融合基因,经PCR、双酶切以及基因测序证实融合基因EBNA1-LMP1已成功插入腺病毒穿梭载体pDC316-mCMV-EGFP;Western blot表明融合基因在293T细胞中表达蛋白EBNA1和LMP1,大小约70 000。动物实验显示,重组腺病毒免疫组的脾CD4+T细胞、CD8+T细胞占总细胞的54.2%和39.2%;小鼠眼球血中重组腺病毒免疫组CD4+T淋巴细胞和CD8+T淋巴细胞占总细胞的54.2%和39.2%;而重组腺病毒免疫组的脾TNF-α和IFN-γ占总细胞因子的0.72%和7.63%,均高于对照组,差异均具有统计学意义(P<0.05)。结论 本实验成功获得了能稳定表达融合蛋白的重组腺病毒,动物实验显示重组腺病毒能有效刺激小鼠体内T淋巴细胞、B淋巴细胞的增殖和细胞因子TNF-α、IFN-γ的分泌。  相似文献   

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目的 研究人癌抗原重组痘苗病毒(rV-CEA)转染上周血树突状细胞(DC)后能否在体外诱导CEA特异性细胞性T淋巴细胞免疫。方法 将rV-CEA转染外周血单核细胞来源的DC后用于激发同源的T细胞,检测其对T细胞的增殖作用以及对CEA分泌性肿瘤细胞的杀伤活性,并与未经rV-CES转染的DC激发的T细胞进行比较。结果 经rV-CEA转染的DC激活的T细胞对CEA分泌性肿瘤细胞具有特异性杀伤作用。结论 rV-CEA转染的DC可以诱导CEA特异性T细胞活性。  相似文献   

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Objective To investigate the antitumor effects of intrasplenically transplanted interleukin-18 (IL-18) gene-modified hepatocytes on murine implanted liver carcinoma. Methods Embryonic murine hepatocyte cell line (BNL-CL2) was transfected with a recombinant adenovirus encoding IL-18 and used as delivery cells for IL-18 gene transfer. Two cell lines, BNL-LacZ and BNL-CL2, were used as controls. One week after intrasplenic injection of C26 cells (colon carcinoma line), tumor-bearing syngeneic mice underwent the intrasplenic transplantation of IL-18 gene-modified hepatocyte cell line and were divided into treatment group (BNL IL-18) and control groups (BNL-LacZ and BNL-CL2 ). Two weeks later, the serum levels of IL-18, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in the implanted liver carcinoma-bearing mice were assayed, the cytotoxicity of murine splenic cytotoxic T-lymphocytes (CTLs) was measured, and the morphology of the hepatic tumors was studied to evaluate the antitumor effects of the approach. Results In the treatment group, the serum levels of IL-18, IFN-γ, TNF-α and NO increased significantly. The splenic CTL activity increased markedly (P&lt;0.01) , accompanied by a substantial decrease in tumor volume and the percentage of tumor area and prolonged survival of liver carcinomo-being mice. Conclusions In vivo IL-18 expression by ex vivo manipulated cells with IL-18 recombinant adenovirus is able to exert potent antitumor effects by inducing a predominantly T-cell-helper type 1 (Th1) immune response. Intrasplenic transplantation of adenovirus-mediated IL-18 gene-modified hepatocytes could be used as a targeting treatment for implanted liver carcinoma.  相似文献   

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目的构建EBV LMP2-LMP1Δ融合基因,初步观察融合基因体内诱导EBV特异性细胞免疫应答的效果。方法 (1)应用PCR方法构建包含EBV-LMP2全长和去除致癌基因的EBV-LMP1Δ融合基因,并将融合基因插入到pcDNA3.1(+)-his真核表达载体中,构建重组表达质粒pcDNA-LMP2-LMP1Δ,Western blot和免疫荧光方法检测融合蛋白的表达。(2)使用pcDNA-LMP2-LMP1Δ及携带LMP1Δ和LMP2基因的重组腺病毒单独或联合免疫Balb/c小鼠,末次免疫1周后应用IFN-γELISPOT方法检测小鼠脾淋巴细胞中EBV特异性CTL水平。结果 (1)真核表达质粒pcDNA-LMP2-LMP1Δ能够在293细胞中表达LMP2-LMP1Δ融合蛋白,融合蛋白分子量大小正确,具有免疫原性。(2)重组质粒pcDNA-LMP2-LMP1Δ免疫小鼠能够诱导出EBV特异性的CTL,但诱导的CTL水平很低,1×106小鼠脾淋巴细胞中平均斑点数只有12个,远远低于LMP1Δ、LMP2重组腺病毒平均495个斑点数的免疫结果。但使用pcDNA-LMP2-LMP1Δ和重组腺病毒联合免疫,与只使用重组腺病毒相比,诱导的EBV特异性CTL水平能够显著提高,1×106小鼠脾淋巴细胞中平均斑点数可达1 001个(P〈0.01)。结论构建的EBV LMP2-LMP1Δ融合基因能够有效表达LMP2-LMP1Δ融合蛋白,并能够在小鼠体内诱导出EBV特异性的CTL反应,与重组腺病毒联合使用,可以提高特异性CTL应答水平。  相似文献   

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Background Survivin is a rather specific gene in tumor tissue.We transfected dendritic cells (DCs) with recombinant adenovirus (Ad) containing survivin gene and granulocyte-macrophage colony-stimulating factor (GM-CSF) gene and tested the inducing effect of the transfected DCs on cytotoxic T lymphocytes (CTL) to kill leukemic cells.Methods After derived from the peripheral,DCs was assayed by mixed leukocyte reaction (MLR) tests.Lactate dehydrogenase (LDH) release test was used to evaluate cytotoxicity of CTL.Results Expression of survivin in transfected DCs was confirmed by Western blotting analysis.GM-CSF expression was confirmed by enzyme-linked immunosorbent assay (ELISA).In MLR assay,DCs coinfected with Ad-survivin and Ad-GM-CSF induced higher allogeneic lymphocyte reaction than control DCs at ratios of 1:5,1:10,1:50 and 1:100.DCs coinfected with Ad-survivin and Ad-GM-CSF had much higher activity of CTL to HL-60 cells than DCs infected with Ad-survivin only,Ad-GM-CSF only,or control DCs.Levels of interleukin-12 (IL-12) and interferon gamma (IFN-y) in lymphocyte supernatants containing DCs coinfected with Ad-survivin and Ad-GM-CSF were significantly higher than those in the control group.Conclusion DCs coinfected with Ad-survivin and Ad-GM-CSF induce much higher anti-leukemic response in vitro than those infected with either factor.Therefore,adenovirus vectors containing survivin and GM-CSF genes may be promising vaccine candidates for leukemia therapy.  相似文献   

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目的:研究小鼠结肠癌细胞CT-26RNA体外转染mIL-12基因修饰的树突细胞(DC),观察其诱导特异性细胞毒性T淋巴细胞(CTL)能力。方法:小鼠骨髓细胞体外经rmGM-CSF、rmIL-4诱导培养获取DC,流式细胞仪检测其纯度;293细胞扩增携带mIL-12基因的重组腺病毒,体外转染DC;Trizol法提取CT-26细胞总RNA,应用TransMessenger体外转染mIL-12基因修饰的DC;ELISA法检测细胞上清及小鼠血液中mIL-12,LDH释放法检测小鼠体内特异性CTL活性。结果:小鼠骨髓细胞经诱导培养后,获得大量高纯度的DC,流式细胞仪检测CD11c^+的DC〉90%;携带mIL-12基因重组腺病毒转染的DC细胞高表达mIL-12;CT-26细胞总RNA体外转染mIL-12基因修饰的DC后,回输小鼠,能明显提高小鼠体内mIL-12的水平,并可诱导体内生成较高水平的CTL活性,而以该RNA转染Ad-LacZ修饰DC后的对照组及RNA转染DC的对照组,可诱导机体生成中等水平的特异性CTL活性,DC、PBS对照组则均无此作用。结论:小鼠肠癌CT-26细胞总RNA转染mIL-12基因修饰的DC后,免疫接种小鼠,可提高小鼠体内mIL-12的水平,并能有效诱导机体产生较高水平的特异性CTL活性。  相似文献   

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目的:制备4种尤文肉瘤树突状细胞(dendritic cell,DC)疫苗,并比较和分析其在体内外抗肿瘤免疫反应中的效果.方法:从健康供者体中分离和培养外周血单核细胞,利用粒-巨噬细胞集落刺激因子(granulocyte-macrophage colony-stimulating factor, GM-CSF)和白细胞介素(interleukin-4,IL-4)将单核细胞诱生为DC,并对其表型进行流式细胞仪分析,分别制备4种DC疫苗:(1)融合瘤,将DC与A673细胞进行电融合,并通过流式细胞仪检测荧光双染色融合瘤,以确定其融合率;(2)负载肿瘤裂解物DC,将DC与A673细胞反复冻融物共培养;(3)EWS/FLI1修饰DC,用编码EWS/FLI1的腺病毒转染DC;(4)未作处理的成熟的DC.将各种DC疫苗与组织相容性白细胞抗原(histocompatibility leukocyte antigen,HLA)相配的CD8 T细胞共培养,用ELISA试剂盒检测刺激增殖细胞毒性T淋巴细胞(cytotoxicity T lymphocyte,CTL)分泌干扰素γ(interferon γ,IFN-γ)的量,用51Cr细胞杀伤试剂盒检测CTL在不同比例下对A673细胞的杀伤作用.通过腹腔注射人外周血淋巴细胞(peripheral blood lymphocyte,PBL),皮下接种A673细胞,构建重建人免疫系统的SCID鼠尤文肉瘤模型,应用ELISA试剂盒检测小鼠外周血中IgG的量,以确定免疫重建效果,并接种不同的DC疫苗,检测其对小鼠成瘤的影响.结果:流式细胞术检测出从外周血单核细胞诱生出的CD83、CD80、CD86及HLA-DR高表达的成熟DC.DCs/A673融合细胞的电融合效率达到16.32%.各种DC疫苗都可以诱导出有效的抗肿瘤免疫反应,IFN-γ分泌检测显示,融合瘤组较其他DC疫苗组产生CTL水平高,其后依次为肿瘤裂解物负载组、EWS/FLI1修饰组和未作处理组.而在体外杀伤A673实验中,融合瘤组杀伤效率最高,肿瘤裂解物负载组次之,EWS/FLI1修饰组与未作处理组最低,且后两组间差异无统计学意义.在抑制肿瘤生长的体内实验中,融合瘤组小鼠肿瘤最小,EWS/FLI1修饰组、肿瘤裂解物负载组和未作处理组之间差别不大.结论:尤文肉瘤DC疫苗可以诱导有效的抗肿瘤免疫反应,将各种尤文肉瘤DC疫苗进行体内外综合比较时发现,融合瘤是最佳的尤文肉瘤免疫疫苗.  相似文献   

19.
ObjectiveTo evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2 (LMP2) modified dendritic cells (DCs) that boosts specific responses of cytotoxic T lymphocytes (CTLs) to LMP2 before and after intradermal injection in patents with nasopharyngeal carcinoma (NPC).MethodsDCs were derived from peripheral blood monocytes of patents with NPC. We prepared LMP2-DCs infected by recombinant adenovirus vector expressing LMP2 (rAd-LMP2). NPC patents were immunized with 2 x 10 LMP2-DCs by intradermal injection at week 0 and after the second and fourth weeks. Specific responses to LMP2 were detected by enzyme-linked immunospot (ELISPOT) assay at week 0 and at the fifth and eighth weeks. Local clinicians performed the follow-up and tracking of patients.ResultsWe demonstrated that DCs derived from monocytes displayed typical DC morphologies; the expression of LMP2 in the LMP2-DCs vaccine was confirmed by immunocytochemical assay. Twenty-nine patients with NPC were enrolled in this clinical trial. The LMP2-DCs vaccine was well tolerated in all of the patients. Boosted responses to LMP2 peptide sub-pools were observed in 18 of the 29 patients with NPC. The follow-up data of 29 immunized patients from April, 2010 to April 2015 indicated a five-year survival rate of 94.4% in responders and 45.5% in non-responders.ConclusionIn this pilot study, we demonstrated that the LMP2-DCs vaccine is safe and effective in patients with NPC. Specific CTLs responses to LMP2 play a certain role in controlling and preventing the recurrence and metastasis of NPC, which warrants further clinical testing.  相似文献   

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