微流控免疫芯片快速检测法的建立及其检测TSH和T4的效果

目的 在已制备的微流控芯片系统上建立基于顺磁微球的免疫检测方法，并将此方法用于检测促甲状腺激素（TSH）和甲状腺素（T4）。方法 采用数字激光刻蚀技术制备微流控芯片，以luminol-H2O2为发光体系，用双抗夹心法测定TSH，用免疫竞争法测定T4。结果 该检测系统可以在25min内完成检测，所需的标本和抗体试剂量为10山，TSH检测范围为0～50μIU／ml，批内CV平均为4．9％，批间CV平均为4．9％，平均回收率为93．74％；T4检测范围为9．375～300ng／ml，批内CV平均为4．9％，批间CV平均为4．7％，平均回收率为91．27％。结论 本方法检测TSH和T4具有操作简便、节约时间、节省试剂、稳定性好、准确度高的优点。     

微流控芯片用于甲胎蛋白化学发光免疫分析的研究

目的：建立一种可用于免疫分析的微流控芯片系统。方法：采用激光直接加工法制备微流控芯片，建立基于超顺磁珠的化学发光微流控免疫分析系统，用于甲胎蛋白（AFP）的测定。结果：芯片系统可在20min内完成AFP的分析，所需要的标本量和试剂量为5μl，线性范围为1～800ng／ml，批内变异系数（CV）平均为6，3％，批间CV平均为7．8％，回收率为94．3％。结论：微流控芯片系统是一种快速、耗费小、可重复使用的AFP测定方法。     

总T3快速检测微流控芯片的制作及应用研究

目的 建立一种可以用于免疫分析的微流控芯片系统。方法 采用激光直接加工法制备微流控芯片，建立由流体控制系统、免疫反应系统和信号处理系统构成的芯片系统，用免疫竞争法测定总T3。结果 芯片系统可以在20min内完成总T3的分析，所需要的标本量和试剂量为5μl。线性范围为1～10ng／ml，批内CV平均为7．2％。批间CV平均为10．7％，回收率为108．5％。结论 微流控芯片系统用于检测总T3具有快速、灵敏、稳定、可重复使用的特点。     

微流控芯片用于AFP快速检测的研究

目的建立一种可以用于免疫分析的微流控芯片系统。方法采用激光直接加工法制备微流控芯片，建立由流体拉制系统、免疫反应系统和信号处理系统构成的芯片系统，用于甲胎蛋白的测定。结果芯片系统可以在20min内完成AFP的分析，所需要的标本量和试荆量为5μl，线性范围为1-800ng／ml，批内CV平均为4．8％，批间CV平均为7．0％，回收率为96．3％。结论微流控芯片系统是一种快速、耗费小、可重复使用的AFP测定方法。     

毛细管电泳与微流控芯片电泳快速检测粪便中腺病毒的方法建立

目的 建立粪便中腺病毒的PCR-毛细管电泳-激光诱导荧光和PCR-微流控芯片电泳-激光诱导荧光快速检测方法。方法 采用试剂盒法提取粪便中腺病毒DNA,选择腺病毒六邻体基因保守区域进行PCR扩增反应。PCR产物分别用高灵敏度的SYBR Gold和SYBR Orange核酸荧光染料进行标记,在优化的毛细管电泳和微流控芯片电泳条件下分离,采用激光诱导荧光检测特异性PCR扩增产物。结果 腺病毒扩增产物在优化的毛细管电泳和微流控芯片电泳条件下可分别于9 min和6 min内完成检测。将PCR扩增产物测序,并与NCBI基因库中核酸序列比对,结果表明所选用的扩增序列特异性良好。商品化DNA Marker毛细管电泳和微流控芯片电泳对目标DNA片段bp值求得的日内精密度分别为1.14%～1.34%和1.18%～1.48%,日间精密度分别为1.27%～2.76%和2.85%～4.06%。毛细管电泳-激光诱导荧光法和微流控芯片电泳-激光诱导荧光法检测腺病毒的灵敏度分别为2.33×10² copies/mL和2.33×10³ copies/mL。两种方法用于实际粪便样品测定,准确度高。结论 本研究建立的PCR-毛细管电泳-激光诱导荧光和PCR-微流控芯片电泳-激光诱导荧光检测方法能够快速、灵敏、准确地检测出粪便中的腺病毒。     

一种基于微流控免疫磁珠快速检测登革热抗原的方法

目的建立一种灵敏、快速、方便的检测登革热抗原新方法,为登革热的临床诊断提供帮助。方法建立了一种基于微流控免疫磁珠快速检测登革热抗原检测方法。使用Solidwork软件设计微流控芯片,采用机械加工法和化学封接方式制备微流控芯片;利用化学偶联反应制备登革热抗体免疫磁珠;在微流控芯片载体上,以HRPTMB-H_2O_2为显色体系,利用双抗体夹心法原理检测登革热NS1抗原;最后,同时应用该方法与传统ELISA试剂盒对57例临床样品进行检测,分析该方法的正确度,并对两种方法的优缺点进行比较。结果微流控免疫磁珠方法整个检测过程仅需20 min,反应体系只需磁珠量10μg和样品量10μL;验证实验中,该方法可以将阴性和阳性明显区分开,阳性样本有显色,阴性、空白样本均无显色;性能上,该新型ELISA方法与传统ELISA方法结果阴阳性符合率达到100%。结论该新型ELISA检测方法具有简便、快速、节省试剂和所需样本少、灵敏度高、稳定性好、准确性高等优点,可以用于登革热抗原检测。     

微流控芯片电泳免疫法检测β_2微球蛋白

目的:建立一种基于微流控芯片电泳技术的快速微量蛋白检测方法。方法:用过量FITC-β2-MG抗体,与分析体系中β2-MG发生非竞争性免疫反应,FITC-β2-MG抗体和免疫反应形成的荧光免疫复合物通过微流控芯片电泳实现分离,激光诱导荧光检测荧光信号。结果:分别考察了电泳缓冲体系、pH值等对芯片电泳行为的影响,结果表明pH8.33、45mmol/LTBE缓冲液分离效果较好,在此基础上实现了β2-MG标准品的分析,建立了微流控芯片电泳免疫分析方法。结论:微流控芯片电泳免疫分析方法可在2min内完成一次样品的电泳分析,且试剂和样品消耗少,灵敏度高,改善了常规免疫分析的不足,显示出较好的应用前景。     

血清促甲状腺素免疫检测技术

血清促甲状腺素（TSH）是反映甲状腺功能最敏感的指标。血清TSH测定方法有多种,从最初的放射免疫分析法和免疫放射分析法到临床逐渐普及的免疫化学发光分析法、电化学发光免疫法和时间分辨荧光免疫法等,乃至近年出现的微流控芯片技术,血清TSH检测的灵敏度显著提高。文章就临床较为常用的血清TSH检测技术及存在的问题作一综述。     

血清促甲状腺素免疫检测技术

血清促甲状腺素(TSH)是反映甲状腺功能最敏感的指标.血清TSH测定方法 有多种,从最初的放射免疫分析法和免疫放射分析法到临床逐渐普及的免疫化学发光分析法、电化学发光免疫法和时间分辨荧光免疫法等,乃至近年出现的微流控芯片技术,血清TSH检测的灵敏度显著提高.文章就临床较为常用的血清TSH检测技术及存在的问题作一综述.     

RGD多肽功能化的微流控芯片识别/捕获肿瘤细胞的研究

目的 探讨经RGD多肽功能化后的微流控芯片系统识别/捕获肿瘤细胞的效果.方法 采用化学共价连接的方法固定RGD多肽,建立由流体控制系统、免疫反应系统构成的细胞识别芯片系统,用于细胞的筛选/捕获;选用不同密度的细胞进行进样试验,考察不同细胞密度对通道内细胞数量的影响,并讨论不同的孵育时间对细胞捕获的影响.结果 随着进样细胞密度的增加,进入通道内的细胞明显增多;此功能化后的芯片系统在15 min内对A549的捕获率为90%,在20min内可完全将细胞捕获于管壁.结论 经RGD多肽修饰的微流控芯片系统可在短时间内对A549细胞进行有效的筛选,且在细胞数量很低时,也能对其进行捕获.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…