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1.
米非司酮配伍米索前列醇用于终止8—26周妊娠的临床观察   总被引:1,自引:0,他引:1  
本文50例孕8-26周妇女用米非司酮配伍米索前例醇终止妊娠,完全流产率94%,不全流产率6%,未发生大出血及感染。胎物排出平均时间为6h53min,其中以孕14^+-20周者最乱,与孕8-14周和孕20^+-26周相比较有显著性差异。90%病例流、引产 量〉100ml,妊娠产物排出后患者阴道出血持续时间〈2周者占84%。  相似文献   

2.
弓形虫感染孕鼠后胎鼠脑病变动态定位研究   总被引:3,自引:1,他引:2  
经孕鼠尾静脉注射弓形虫NT强毒株滋养体孵育液后分批处死,观察胎鼠脑动病理变化。结果表明,胎鼠脑病变主要为神经细胞变性、坏死、数量减少,胶质细胞明显增生。孕鼠感染后2小时,免疫组化即可在胎鼠脑组织内发现弓形虫。提示,先天性弓形虫感染的新生铆出现的中枢神经系统发育异常,是由于弓形虫对大脑神经细胞的损害所致;免疫组化显示组织、细胞内的弓形虫是一个简便、准确的诊断方法。  相似文献   

3.
NK细胞,LAK细胞抗弓形虫作用机理的研究   总被引:1,自引:0,他引:1  
观察IL-2在小鼠抗弓形虫机理中的作用。方法:应用^3H-尿嘧啶(^3H-U)特异性标记弓形虫在小鼠腹腔巨噬细胞(Mouseperitonealmacrophage,MPM)内的增殖实验及^125I-尿嘧啶核苷(^125U-UdR)标记的细胞毒实验,观察IL-2的抗弓虫作用。结果IL-2对MPM的抗弓形虫作用及对IFN-γ诱导的MPM抗弓形虫作用无明显影响;正常小鼠NK细胞杀伤弓形虫感染靶细胞的能  相似文献   

4.
探讨β2-糖蛋白I在体内的分布。方法:经昆明系小鼠为实验模型,将纯化的小鼠β2-GPⅠ用Na^125I标记注入到小鼠体内,80min后取一些重要脏器做放射性计数测定。结果:Na^125-0I,β2-GP-Ⅰ放射性计数在血浆中为215.01±16.45Bq.g^-1,在肾脏中为51.57±6.30bQ.G^-1,在肾脏中为49.38±5.14Bq.g^-1,在肺中为11.96±1.52Bq.g^-1  相似文献   

5.
目的建立经口感染弓形虫RH株速殖子垂直传播小鼠模型。方法将54只妊娠0天的BALB/c孕鼠随机分为6组,于妊娠第8天,分别用0,1000,2000,4000,8000,16000个弓形虫速殖子灌胃感染,妊娠第18天处死,记录活胎率,测定母鼠肝、胎盘和胎仔脑组织弓形虫抗原。结果经ELISA检测弓形虫循环抗原证实,孕鼠死亡率与胎盘和胚胎感染率均随感染剂量的增加而升高,而活胎率下降。1000,2000组的胎盘、胚胎未发生感染,4000组的胚胎感染率5.13%,16000组的胚胎感染率20.00%,但孕鼠的健康状况差、死亡率高,均不适宜建立弓形虫垂直传播动物模型。综合分析表明,8000组孕鼠既有较高的存活率,又可保证胎盘和胚胎较高的感染率。结论每只孕鼠灌胃感染8000个速殖子为建立弓形虫垂直传播小鼠模型的适宜剂量。  相似文献   

6.
为探讨高兹放活性血管活性肠肽(^125I-VIP)在正常小鼠体内的分布特性,取纯系昆明种小鼠30只,随机分为6组,每组5只,在尾静脉注入^125I-VIP0.2ml(74kBq)后180分钟内的不同时相,将小鼠断头处死,收集血液、肺、肝、肠和肾,称重并测定其放射性,最后换算为每克组织的ID%。另取纯系昆明种小鼠30只,分组同上,尾静脉分别注入含10、20和40μgVIP的^125I-VIP溶液,于  相似文献   

7.
朱燕飞 《四川医学》1999,20(5):464-465
我院自1995年1月至1998年7月对自愿接受无痛分娩的孕足月、单胎、头位、初产妇66例,分娩时使用连续硬膜外阻滞,效果良好。报道分析如下。1 临床资料1.1 对象:孕足月、单胎、头位、初产妇,骨盆内测量各径线无异常,无明显头盆不称,自愿接受经阴道无痛分娩者,共计66例。1.2 方法:于临产后宫口开大3~4cm,活跃早期行硬膜外穿刺,穿刺点选择L2~3或L3~4椎间隙并向上置管在硬膜外腔保留导管3.5cm。1.3 药物:0.125%的布比卡因加芬太尼100μg布比卡因和芬太尼混合液,首次给药8m…  相似文献   

8.
孕妇和胎儿弓形虫感染抗体检测的研究   总被引:1,自引:0,他引:1  
目的为了解银川地区孕妇和胎儿弓形虫感染状况及与职业、民族和孕产次之间的关系,以保障孕妇和胎儿的身心健康。方法用间接血凝试验对279例孕妇静脉血和胎儿脐血配对及33例孕妇静脉血进行检测。结果303名孕妇,弓形虫抗体阳性21人,阳性率为6.9%;279份胎血中阳性6份,阳性率为2.2%;279份母血和胎血配对检测中母血阳性而胎血阴性15人,占5.4%。干部,工人,农民,无固定职业四组弓形虫抗体检测各组阳性率分别为5.9%、7.5%、8.3%和9.7%,差异无显著性(P>0.05)。回汉族孕妇弓形虫抗体阳性率分别为5.6%和8.9%,差异无显著性(P>0.05)。孕妇第一产次弓形虫抗体阳性明显高于第二、三产次;弓形虫抗体阳性均出现在第一产次胎儿脐血中。结论不同职业和民族的孕妇均有弓形虫的感染,母体感染后可经胎盘血液传递给胎儿使其感染,第一产次孕妇感染后使胎儿受染机会大于第二、三产次。对优生、优育,围产期保健工作应给予高度重视。  相似文献   

9.
碱性成纤维细胞生长因子对胎盘屏障通透性的实验研究   总被引:1,自引:0,他引:1  
目的:探讨碱性成纤维细胞生长因子(bFGF)能否通过胎盘屏障而对胎儿发挥其神经营养作用。方法:怀孕16d(孕中期)Wistar孕鼠,用^125I标记的bFGF(^125I-bFGF)经腹腔注射,30min后取孕鼠血清及胎鼠脑、心、肝、脾、肺,检测和比较各组织bFGF的放射活性,并以同一注射剂量的^125I-bFGF在肝脏的分布含量为参照,比较胎鼠脑、新生鼠脑和成年鼠脑与肝脏含量比。结果:(1)胎鼠体内有^125I-bFGF的分布,且随外源性^125I-bFGF注射剂量增加而升高。(2)在安全剂量范围(5-500ng/g)内,外源bFGF剂量增加到一定值(150ng/g)时,胎盘屏障通透性明显提高。(3)注射相同剂量^125I-bFGF,与胎鼠其他器官相比,胎鼠脑组织内^125I-bFGF含量很少。(4)以肝组织^125I-bFGF含量作参照,与新生鼠脑及成年鼠脑相比,外源性^125I-bFGF在胎鼠脑含量最高。结论:bFGF能够部分通过胎盘屏障进入胎脑。  相似文献   

10.
用Bolton-Hunter试剂联结法标记胆囊收缩素(CCK8),得^125I-BH-CCK8,其比放射性为3.4TBq/mmol,放射化学纯度大于96%,取其与自制的大鼠大脑皮质细胞膜进行受体放射分析,发现标记配体与大鼠大脑皮质CCK受体的结合具有温度和时间依赖性,可饱和性,可逆性及特异性,经Scatchrad分析获大鼠大脑皮质细胞膜CCK受体Kd值为1.098nmol/L,Bmax为197.5  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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