白介素1对鼠甲状腺细胞钙离子的作用

目的：利用激光扫描共聚焦显微镜（ＬＳＣＭ）观察ＩＬ－１β及ＴＳＨ对鼠甲状腺ＦＲＴＬ－５细胞内Ｃａ２＋的影响，以进一步了解ＩＬ－１β对甲状腺细胞的作用及机制。方法：将待测ＦＲＴＬ－５细胞以Ｆｌｕｏ－３负载，以ＬＳＣＭ扫描测定细胞内Ｃａ２＋，分别加入不同浓度的ＴＳＨ或ＩＬ－１，观察细胞内Ｃａ２＋变化。结果：（１）ＴＳＨ可使细胞内Ｃａ２＋的水平上升，与ＴＳＨ浓度有关。（２）ＩＬ－１β可降低ＦＲＴＬ－５细胞内基础Ｃａ２＋和ＴＳＨ刺激的细胞内Ｃａ２＋的水平。结论：ＴＳＨ对甲状腺细胞磷酸肌醇／钙离子系统有刺激作用，Ｃａ２＋作为第二信使可介导ＴＳＨ的效应。Ｉ－１β可以通过抑制甲状腺细胞磷酸肌醇／钙离子系统而发挥其抑制效应     

树突状细胞刺激γδT细胞扩增诱导干细胞移植时GVL研究

目的：研究γδＴ细胞的体外生存期,抗白血病活性及可能引起的移植物抗宿主反应（ＧＶＨＤ）,为自体及异体移植后输注γδＴ细胞诱导移植物抗白血病反应作前期基础研究。方法：对移植患者采集物用ＧＭ－ＣＳＦ＋ＩＬ－４进行培养,使其分化和扩增为树突状细胞（ＤＣ）;然后用ＤＣ和细胞因子ＩＬ－２刺激其ＣＤ４＾＋Ｔ细胞扩增后,协同ＤＣ共同刺激γδＴ细胞扩增,进而用ＭＴＴ比色法和ＣＦＵ－ＧＭ集落培养研究其功能。结果：γ     

GM—CSF诱导红白血病细胞向树突状细胞分化及其抗原提呈功能的研究

目的探讨粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）诱导小鼠红白血病细胞分化过程中，树突状细胞的产生及其抗原提呈功能的变化。方法采用流式细胞仪分析、电镜技术和４小时５１Ｃｒ释放法等，进一步观察了ＧＭ－ＣＳＦ的诱导分化作用。结果１００ｎｇ／ｍｌＧＭ－ＣＳＦ处理３天后的ＦＢＬ－３细胞，树突状细胞的特异性标志３３Ｄ１和ＮＬＤＣ１４５的表达阳性率显著升高；同时，ＭＨＣ－Ⅱ、Ｂ７－１、Ｂ７－２、Ｉ－ＣＡＭ－１、ＶＣＡＭ－１和ＣＤ４０表达水平均增加；扫描电镜显示ＧＭ－ＣＳＦ处理的ＦＢＬ－３细胞，表面出现许多树突状突起，透射电镜下可见细胞浆内有丰富的线粒体，核呈分叶状。同时，能够显著刺激同种异体Ｔ淋巴细胞增殖，促进ＩＬ－２的产生；可显著提高细胞毒性Ｔ淋巴细胞（ＣＴＬ）细胞对ＦＢＬ－３细胞的特异性杀伤活性。结论ＧＭ－ＣＳＦ诱导的红白血病细胞可向树突状细胞分化，本身具有抗原提呈功能。     

P物质对大鼠脾细胞免疫功能的影响

目的：研究Ｐ物质（ＳＰ）对脾细胞免疫功能的调节作用。方法：采用３Ｈ－ＴｄＲ掺入法、放射免疫测定和流式细胞仪等方法,检测了ＳＰ对体外培养的大鼠脾细胞的增殖和分泌ＩＬ－２、ＩＬ－６、ＴＮＦ功能及Ｔ细胞表型表达的影响。结果：ＳＰ能明显刺激脾细胞的增殖,其中浓度为１×１０－ ４ｍ ｍ ｏｌ／Ｌ时作用最强;ＳＰ能促进Ｔ细胞ＣＤ分子的表达,使ＣＤ４＋ ／ＣＤ８＋ 升高;ＳＰ能显著促进脾细胞分泌ＩＬ－２,对脾细胞分泌ＩＬ－６具有抑制作用,而对分泌ＴＮＦ无作用。结论：证实了ＳＰ是具有广泛的免疫调节作用的一种神经肽。     

不同细胞因子对培养人外周血树突状细胞的影响

目的建立从人外周血分离、纯化、培养、扩增树突状细胞（ＤＣ）前体的方法，研究细胞因子对ＤＣ体外增殖、分化成熟的影响。方法人外周血经血细胞分离仪及Ｆｉｃｏｌ、Ｐｅｒｃｏｌ等不连续密度梯度离心获得的含ＤＣ前体细胞组分用重组人粒细胞／巨噬细胞集落刺激因子（ｈｒＧＭ－ＣＳＦ）培养或用ＧＭ－ＣＳＦ及白细胞介素－４（ＩＬ－４）联合培养；光镜及电镜观察及ＡＢＣ法免疫细胞化学染色。结果分离的ＤＣ前体经１周左右时间培养，细胞数量可扩增２０～３０倍，纯度达９０％以上，ＧＭ－ＣＳＦ与ＩＬ－４联合培养所得到的ＤＣ数量约为用ＧＭ－ＣＳＦ培养的１．５倍。ＤＣ具有典型的树枝状或裙褶状突起，并表达高水平的ＨＬＡ－ＤＲ，其ＣＤ１４、ＣＤ１９、ＣＤ３的表达均为阴性。结论用ＧＭ－ＣＳＦ和ＩＬ－４联合作用更能促进ＤＣ的体外扩增及分化。     

转化生长因子β1基因修饰的小鼠树突状细胞在移植体内的迁移？…

目的 观察β型转化生长因子（ＴＧＦβ１）基因修饰的Ｂ１０小鼠骨髓树突关细胞（ＤＣ）在Ｃ３Ｈ小鼠体内的迁移和存活,以探讨ＤＣ生物学特性与移植耐受性的关系。方法 从Ｂ１０小鼠骨髓分离ＤＣ前体细胞,在粒细胞－巨噬细胞集落刺激因子＋β型转化生长因子（ＧＭ－ＣＳＦ＋ＴＧＦβ）条件下培养增殖（ＧＣ１）并转导Ａｄ－ＬａｃＺ（Ｄｃ２）或Ａｄ－ＴＧＦβ１（ＤＣ３）,每组５×１０＾５个细胞注入Ｇ３Ｈ小鼠后掌皮下,分别     

造血生长因子对长期培养中骨髓造血细胞增殖和分化的影响

应用骨髓长期培养法研究了ＨＧＦｓ联合应用对ＬＴＢＭＣ中造血干／祖细胞的增殖、分化的影响，结果显示，在ＬＴＢＭＣ中，含有ＨＧＦｓ（ＩＬ－２＋ＩＬ－６＋Ｇ－ＣＳＦ＋ＧＭ－ＣＳＦ＋Ｅｐｏ）的扩增组与对照组相比，ＨＧＦｓ能显著地扩增ＨＳＣｓ，在第一周，ＣＦＵ－ＧＭ，ＣＦＵ－Ｅ和ＢＦＵ－Ｅ总数分别扩增１８．０９－１６．５９倍、１０．２６－８．４８和１４．９９－１２．７８倍，而累积扩增倍数分别达３６．１９－６     

抗人粒细胞集落刺激因子单克隆抗体的研制

应用杂交瘤技术，用重组人Ｇ－ＣＳＦ（ｒｈＧ－ＣＳＦ）免疫Ｂａｌｂ／ｃ小鼠，然后将其脾脏细胞与小鼠骨髓瘤细胞ＮＳ－１融合，成功地获得了多种能够分泌抗ｒｈＧ－ＣＳＦ单克隆抗体（ＭｃＡｂ）的杂交癌细胞。对其中的２株杂交瘤细胞２Ａ６和２Ｃ２分泌ＭｃＡｂ的研究表明，它们能够特异性地和ｒｈＧ－ＣＳＦ结合，而与重组的人ＧＭ－ＣＳＦ、ＩＬ－ＣＳＦ、ＩＬ－２、ＩＬ－２α和ＴＮＦ细胞因子无交叉反应，为抗Ｇ－ＣＳＦ特异性ＭｃＡｂ。这些杂交瘤细胞经反复冻存和复苏后，仍能稳定地分泌ＭｃＡｂ。     

人外周血树突状细胞的诱导及其生物学特性的研究

目的：建立体外诱导和扩增人外周血树突状细胞（ＤＣ） 的方法,并分析其生物学特性。方法：造血动员后外周血单个核细胞经贴壁去除悬浮细胞,加入细胞因子（ＩＬ－４、ＧＭ－ ＣＳＦ和ＴＮＦ－α）培养８ 天。用流式细胞仪分析细胞的表型,经ＥＬＩＳＡ法测定其培养上清中的ＩＬ－ １２ ,并将诱导的树突状细胞与脐带血原始Ｔ（ｎａｉｖｅ Ｔ） 细胞混合培养,用３Ｈ－ ＴｄＲ掺入法测定细胞的增殖指数。结果：贴壁的造血动员后外周血单个核细胞在体外经细胞因子诱导培养后,高表达人树突状细胞分化抗原ＣＤ１ａ（８９ ．１％ ）、ＣＤ４０（９９ ．８％ ）、ＣＤ８０（９５ ．１％ ）、ＣＤ８３（４５ ．７％ ）、ＨＬＡ－ ＤＲ（９７．６％ ）,同时所获的ＤＣ能分泌ＩＬ－１２ 和可有效地激活脐带血原始Ｔ细胞并促其增殖（ＳＩ＝ ６．９２） 。结论：采用造血动员的外周血经体外贴壁去除非粘附细胞,再经过细胞因子序贯培养,无需纯化ＣＤ３４ ＋ 细胞,能获得高纯度（ ＞８０％ ）和具有功能性的ＤＣ细胞。     

脑胶质瘤浸润淋巴细胞的分离、培养与表型及体外抗肿瘤活性测定

经重组白细胞介素２（ｒＩＬ－２）和胸腺因子Ｄ（ＴＦＤ）培养前后的人脑胶质瘤浸润淋巴细胞（ＧＩＬ）的表型及抗自体瘤细胞活性变化。（１）ＧＩＬ可被分离，培养后可增值；（２）新鲜ＧＩＬ主要为Ｔ淋巴细胞，其中ＣＤ＋８细胞多于ＣＤ４细胞，随培养时间延长呈相反变化；（３）新鲜ＧＩＬ活性很弱，培养后很强；（４）ＴＦＤ可能只有增强ｒＩＬ－２的激活作用。提示ＧＩＬ可望作为理想的效应细胞用于脑胶质瘤的治疗。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…