化痔灵片对小鼠实验性疼痛的镇痛作用

目的 观察化痔灵片对小鼠实验性疼痛的镇痛作用。方法 采用小鼠腹腔注射醋酸致痛法及小鼠热板法疼痛模型，观察化痔灵片对小鼠扭体反应次数及热痛阈值的影响。结果 化痔灵片3g／kg、10g／kg灌胃给药5d使小鼠腹腔注射0．7％（体积分数）醋酸的扭体反应次数较盐水组明显减少（P〈0．05），对扭体反应次数的抑制率分别为38．4％和46．6％；使小鼠热板法痛阈较药前显著提高（P〈0．05），药后痛阈与盐水组比较显著提高（P〈0．05）。结论 化痔灵片能显著减少小鼠腹腔注射醋酸的扭体反应次数，提高小鼠热板痛阈，提示其具有一定的镇痛作用。     

荆芥内酯聚乳酸乙醇酸纳米粒的抗炎、镇痛及解热作用

目的:研究荆芥内酯聚乳酸乙醇酸纳米粒(SCH-PLGA-NP)的初步药效学作用.方法:用二甲苯致小鼠耳廓肿胀及腹腔注射0.6%醋酸致小鼠腹腔染料渗出的方法制作急性炎症模型,观察SCH-PLGA-NP对急性炎症的影响;用醋酸致痛扭体法及热板法观察镇痛作用;用10%的干酵母混悬液致大鼠发热模型,观察SCH-PLGA-NP对酵母致大鼠发热体温的影响.结果:SCH-PLGA-NP对小鼠耳廓肿胀度和腹腔毛细血管通透性的增加有明显的抑制作用;SCH-PLGA-NP能明显减少小鼠的扭体反应数;在给药后(热板法)15,30,60 min均能显著延长小鼠疼痛反应的潜伏期,痛阙值提高;SCH-PLGA-NP对酵母致大鼠发热有明显的降低作用.结论:SCH-PLGA-NP具有较好的抗急性炎症、镇痛和解热作用,且抗炎、镇痛和解热效果明显优于游离荆芥内酯.     

大高良姜根茎提取物对小鼠的镇痛作用

目的：评价大高良姜（Alpinia galanga）根茎的乙醇提取物对小鼠的镇痛作用并推断其可能的机制。方法：使用3个实验模型分别测定大高良姜根茎提取物对白化病小鼠（体质量25～30g）的镇痛作用。热板实验中,30只小鼠被分为5组,每组6只,分别给予口服3种剂量的大高良姜根茎乙醇提取物、皮下注射吗啡以及口服2%的阿拉伯树胶,记录给药30、60、90和120min后的热板实验反应时间。纳洛酮预处理热板实验中,另外30只小鼠在给予上述药物30min之前皮下注射纳洛酮,给药种类及方式同前,热板实验反应时间记录方式同前。扭体实验中,通过腹腔注射醋酸诱导小鼠产生扭体反应,随后30只小鼠被分为5组,每组6只,分别给予口服3种剂量的大高良姜根茎乙醇提取物、阿司匹林及2%的阿拉伯树胶,每只小鼠观察15min,记录扭体次数。结果：各个时间记录点的数据表明,与对照组相比,3种剂量的大高良姜根茎乙醇提取物均显著延长了热板实验中小鼠的反应时间（P〈0.05,P〈0.01）。经纳洛酮预处理后,实验组及阳性对照药吗啡组小鼠的热板实验反应时间较对应的前一实验中未经纳洛酮处理各组小鼠显著延长（P〈0.05,P〈0.01）。扭体实验中,与对照组相比,3种剂量的大高良姜根茎乙醇提取物均减少了实验小鼠的扭体次数（P〈0.01）。结论：本实验证实了大高良姜根茎提取物的镇痛作用,验证了其在民族医学中治疗各种原因引起的疼痛的应用。其镇痛作用的机制可能是有效成分作用于中枢或外周神经系统     

辣椒丙酮温浸提取物镇痛作用研究

【目的】研究丙酮温浸提取工艺得到的辣椒提取物灌胃给药后的镇痛作用。【方法】以阿司匹林为阳性对照药，采用热板法、热水缩尾法和扭体法3种疼痛模型，观察辣椒提取物对小鼠疼痛反应潜伏期和小鼠扭体次数的影响。【结果】辣椒丙酮温浸提取物灌胃给药后能明显延长小鼠的疼痛反应潜伏期，减少小鼠扭体次数，且作用呈剂量相关性。【结论】辣椒丙酮温浸提取物具有显的镇痛作用。     

祛风息痛丸对小鼠实验性疼痛的镇痛作用

目的 观察祛风息痛丸的镇痛作用,为其临床应用提供一定的实验依据. 方法 制备小鼠实验性疼痛模型,观察祛风息痛丸的镇痛作用.①扭体法:取昆明种小鼠100只,随机分为5组,每组20只,雌雄各半.对照组灌胃给予生理盐水10 ml/kg;祛风息痛丸低、中、高剂量组分别灌胃祛风息痛丸0.36,1.08,3.24 g/kg.阳性对照组灌胃阿司匹林0.4 g/kg.每Et用药1次,10 d后观察各组动物腹腔注射醋酸后5-20 min内的扭体反应次数.②热板法将小鼠放在(55±1)℃的热板上,以小鼠舔后足为疼痛反应指标,观察小鼠的热痛阈值.选用痛觉阈值在5-30 s的雌性小鼠50只,随机分为5组,每组10只,分组与给药方法与①同.给药10 d后记录各组小鼠热板法痛阈值,比较各组间的差异. 结果 ①扭体法实验结果显示,祛风息痛丸0.36,1.08,3.24 g/kg灌胃10 d使腹腔注射醋酸所致的小鼠扭体反应次数较对照组明显减少,对扭体反应次数的抑制率分别为25.4%,30.0%和43.4%(P<0.05).②热板法实验结果显示,给药前各组小鼠热痛阈值无统计学差异(P>0.05);给药10 d后祛风息痛丸各剂量组小鼠热痛阈值均较给药前显著提高(P<0.05),与对照组比较,祛风息痛丸组给药后热痛阈值显著延长(P<0.05). 结论 祛风息痛丸对小鼠实验性疼痛具有良好的镇痛作用.     

竹叶莲镇痛作用的实验研究

目的 探讨竹叶莲提取物的镇痛作用.方法 采用小鼠热板法、小鼠醋酸扭体法,观察不同剂量的竹叶莲水提物对小鼠灌胃给药的镇痛作用.结果 竹叶莲水提物高、低剂量均可使热板所致小鼠舔足反应潜伏期明显延长（P＜0.01）,舔足潜伏期延长率最高达66.42％;可使醋酸所致小鼠扭体潜伏期明显延长（P＜0.05）,扭体次数明显减少（P＜0.01）,扭体抑制度最高达39.84％.结论 竹叶莲具有明显的镇痛作用.     

囊泡谷氨酸转运体抑制剂芝加哥天蓝6B的镇痛作用

目的 研究抑制囊泡谷氨酸转运体(vesicular glutamate transporters,VGLUT)功能对动物疼痛行为的影响。方法分别观察选择性VGLUT抑制剂芝加哥天蓝6B(Chicago sky blue 6B,CSB6B)对小鼠热板潜伏期、醋酸扭体次数和福尔马林所致舔足时间的影响。结果 腹腔注射CSB6B不影响小鼠热板潜伏期。小鼠醋酸扭体实验中,与溶剂对照组相比,CSB6B 2.5mg/kg腹腔注射30 min后可使小鼠扭体次数从26.50±2.97显著下降到8.22±1.90(P<0.01),腹腔注射60 min后可使小鼠扭体次数显著下降到9.60±1.84(P<0.01)。在福尔马林实验中,与溶剂对照组相比,CSB6B不影响小鼠的Ⅰ相舔足时间,CSB6B 0.5 mg/kg腹腔注射30 min后可使小鼠的Ⅱ相舔足时间从(139.40±21.02)s显著下降到(75.10±19.45)s(P<0.05),而注射2 h后对小鼠舔足时间没有显著影响。结论 抑制VGLUT功能可显著抑制炎性疼痛,提示VGLUT参与了炎性疼痛的调控,可能成为疼痛治疗的新靶点。     

大果木姜子镇痛作用活性部位筛选

目的研究大果木姜子镇痛作用的成分和机制。方法昆明种小鼠随机分为正常对照组、安慰剂组、阿司匹林组、曲马多组和大果木姜子石油醚、二氯甲烷、正丁醇提取部位的高、中、低剂量组。除正常组外,各组灌胃给药,安慰剂组灌服生理盐水。①扭体法:给药后45min腹腔注射0.6%醋酸0.2mL/g,观察记录给药后15min内小鼠扭体次数。②热板法:雌性小鼠,灌胃给药2h后,以热板法测定小鼠的痛觉反应时间。③甲醛法:给药2h后,小鼠左后足底注射20μL2.5%福尔马林,测定小鼠5～10min、20～30min的痛觉反应。结果①大果木姜子各提取部位对醋酸诱发小鼠扭体反应有显著的镇痛作用,与安慰剂组比较,石油醚提取部位对小鼠的扭体次数减少明显(P0.01)。②大果木姜子石油醚提取部位、二氯甲烷提取部位对热板法致痛有显著的镇痛作用,与安慰剂组比较,能显著提高小鼠的痛阈值(P0.01)。③大果木姜子石油醚提取部位对甲醛致痛有显著的镇痛作用,能明显减少小鼠5～10min(I相)和20～30min(II相)的痛觉反应时间,与安慰剂组比较,镇痛效果有显著性差异(P0.05或P0.01)。结论大果木姜子脂溶性成分具有镇痛作用,其镇痛作用机制可能与中枢机制有关。     

皱皮木瓜注射液镇痛抗炎作用的实验研究

目的 观察皱皮木瓜注射液(MGI)的镇痛抗炎作用.方法 采用热板法测定MGI对小鼠镇痛阈值的影响,扭体法测定MGI 对小鼠扭体反应的潜伏期和扭体次数的影响;观察醋酸致炎后MGI对小鼠腹腔毛细血管通透性的影响;采用蛋清致大鼠足肿胀关节炎模型,观察MGI对大鼠足肿胀的影响.结果 MGI对小鼠热板法和扭体法疼痛模型均有显著镇痛作用;0.80g/kg对热板法90min时的痛阈提高率达49.5%;对醋酸致小鼠扭体疼痛痛阈提高率达88.7 %;对小鼠毛细血管通透性增高和大鼠蛋清性足肿胀均有显著的抑制作用.结论 MGI具有肯定的抗炎镇痛作用.     

仙人掌提取物对小鼠镇痛作用的实验研究

目的　观察仙人掌提取物对小鼠的镇痛作用。方法　采用扭体法和温浴法分别对小鼠腹腔注射仙人掌提取物(仙人掌组 )、阿斯匹林 (阿司匹林组 )及等容量生理盐水 (生理盐水组 ) 1ml,仙人掌组又分别予 0 .5ml、1ml不同剂量给药 ,比较各组扭体数和痛阈潜伏期的变化。结果　以仙人掌提取物对扭体法致痛的小鼠镇痛作用明显优于阿斯匹林及生理盐水 ,χ2 分别为 4.8,15 .0 ,P <0 .0 5或 0 .0 0 1,温浴法给药后不同时间痛阈潜伏期也明显长于阿斯匹林组和生理盐水组 ,P <0 .0 5或 0 .0 1。结论　仙人掌提取物对小鼠具有显著的镇痛作用     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…