银川市某医院体检人群EV71型和ECHO病毒感染率的调查

目的了解银川市不同年龄组人群肠道病毒71型(EV71型)和埃克病毒(ECHO)感染率情况及IgG抗体阳性率分布情况。方法采集宁夏银川市某医院204份不同年龄组体检人群血清,用间接酶联免疫吸附试验(ELISA)检测血清中的EV71型和ECHO IgG抗体。结果 204例受检人群血清中EV71型和ECHO IgG阳性率分别为32.4%和15.1%,其中1岁以内婴幼儿EV71抗体阳性率低,1岁～组EV71 IgG抗体阳性率最高达48.3%,但与其他组比较差异无统计学意义。1岁以内婴幼儿ECHO抗体阳性率低,随着年龄增长,抗体阳性率增高,15～65岁组ECHO IgG抗体阳性率最高,为22.3%,与其他组比较差异无统计学意义。结论普通人群中可能存在EV71型和ECHO隐性感染者,成人EV71隐性感染率较低,儿童是EV71的主要感染者,15岁以下儿童为ECHO感染的高危人群,人群通过自然感染获得免疫保护。     

登革热疫点22年后的血清流行病学调查

目的了解曾发生登革热流行的旧疫区健康人群抗登革病毒的抗体水平及分布。方法在1986年曾爆发Ⅱ型登革热疫情的黄埔区鱼珠街一带,对2周内无临床症状的健康人群横断面采样,收集血清,ELISA法检测血清中抗登革病毒IgG抗体,并和非疫点健康人群抗登革病毒IgG抗体阳性率进行比较。RT—PCR法检测抗体阳性者血清中登革病毒。结果登革热疫点健康人群抗登革病毒IgG抗体阳性率为23.3％,非疫点健康人群抗登革病毒IgG抗体阳性率为3．8％,两者差异有统计学意义（P〈0．01）;登革热疫点人群抗体阳性率与年龄成正相关,0～20、21～30、31—40、41～50、51—60、61～70和70岁以上各年龄组的抗体阳性率分别为3．8％、5．8％、9．1％、26．5％、27．7％、32．5％和35．1％。30岁以下年龄组与31～40岁年龄组、31～d0岁年龄组与41～60岁年龄组、41-60岁年龄组与61岁年龄组间的抗登革病毒IgG抗体阳性率差异分别具有统计学意义（P〈0．05）。登革热疫点和非疫点15岁以下中小学生滤纸血样标本中均未检测到抗登革病毒IgG抗体。RT—PCR法未检测到抗体阳性者血清中有登革病毒。结论登革热疫点人群抗登革病毒IgG阳性率明显高于非疫点人群,疫点人群抗体阳性率随年龄增长而上升,提示登革热疫点在登革热疫情后,登革病毒可能在蚊媒体内存在低密度的循环。     

龙岗区2003年人群乙脑抗体水平监测结果分析

目的 了解龙岗区健康人群乙脑抗体水平，为有效预防乙脑提供科学依据。方法：采用横断面研究，按整群抽样的方法，于2003年7～9月在龙岗区的2所幼儿园、2所学校及从业人员中采集不同年龄人群血清标本，应用间接法ELISA检测乙脑抗体。结果共采集不同年龄人群血清标本246份，乙脑抗体阳性17人，抗体阳性率为6．9％，其中，〈10岁年龄组的抗体阳性率最高，为22．2％，其他年龄组的抗体阳性率为1．2％．2．5％之间，各年龄组抗体阳性率差异有统计学意义（P〈0．001）；男性抗体阳性率为8．7％，女性抗体阳性率为5．6％，差异没有统计学意义（P〉0．05）。结论龙岗区健康人群乙脑抗体阳性率较低，要警惕乙脑流行。建议提高乙脑疫苗免疫接种率；开展乙脑监测工作；加大防蚊、灭蚊力度。     

丹阳市人群麻疹免疫状况调查分析

目的调查丹阳市不同人群的麻疹抗体水平，为麻疹防制工作提供依据。方法采用ELISA法对随机抽取的998份健康人群血清进行麻疹抗体水平检测。结果人群麻疹IgG抗体阳性率和GMT处于中等水平，分别为91．68％和1：730．7；〈8月龄儿童的抗体水平最低，抗体阳性率和GMT分别为30．77％，1：6．42；其次为15岁-年龄组和10岁-年龄组，抗体阳性率和GMT分别为95．73％，1：634．2和97．69％，1：804．5；麻疹抗体阳性率在男女性别和城乡间差别无显著性。结论丹阳市健康人群对麻疹有一定的免疫力，近期不会发生大面积的麻疹流行，但是应重视麻疹的预防与控制，对15岁碑龄组和10岁。年龄组人群进行麻疹疫苗的加强免疫，提高抗体水平。     

潮汕地区EV71流行特征分析

目的了解潮汕地区儿童肠道病毒71型（EV71）血清流行病学、病原学及临床特征。方法收集不同年龄健康儿童血清,采用微量中和试验方法检测其血清EV71中和抗体的阳性率和几何平均滴度（geometric mean titeGMT）。筛选HFMD患者328例,采用荧光定量RT-PCR检测肠道病毒核酸,并收集相关临床资料综合分析。结果在各年龄组中,0-6个月儿童中和抗体阳性率为78.4%,几何平均滴度为1：36.6;7-12个月血清抗体阳性率则显著下降,为28.4%,GMT为1：8.9。从1岁开始血清抗体阳性率和GMT均有所升高,在2-4岁组阳性率继续升高,而在5-14岁组,阳性率急剧升高为79.3%,GMT为1：71.9。以上各年龄组男女性别间的差别均无统计学意义（P〉0.05）。在所监测的328例HFMD病例中,EV71是主要病原体,是引起重症HFMD的主要病因。结论 EV71在潮汕地区流行情况严重,7-12个月儿童EV71中和抗体水平显著低于其他年龄段的儿童,是EV71感染的高危群体。中和抗体水平偏低可能是引起重症HFMD和死亡病例的主要原因。     

包头市2006年流感病毒抗体监测结果

目的：了解包头市2006年流感抗体水平,为科学预防流感提供依据。方法：采用微量半加敏红细胞抑制试验,对包头医学院第一附属医院检验科采血室收集的454份空腹静脉血清进行流感抗体检测,对包头市人群流感抗体水平进行分析。结果：检测人群H5N1阳性率为62．6％,以5～15岁（78．3％）和15～25岁（73．3％）年龄组较高;H9N2阳性率为36．0％,以25～60岁年龄组最高;A1／上海／7／1999（HIN1）亚型阳性率为38．2％,以5～15岁年龄组最高;B／浙江／2／2001（Victoria）阳性率为71．0％,以5～15岁年龄组最高。结论：2006年包头市H5N1型、B／浙江／2／2001（Vic—toria）型流感抗体水平较高,不易引起相应病毒型流感的大流行,婴幼儿和老年人抗体水平相对较低,建议在流行期前进行预防接种。     

清远市无SARS病例地区人群SARS冠状病毒抗体血清流行病学调查

目的探讨清远市无SAILS病例地区人群SAILS冠状病毒（SARS-CoV）抗体水平。方法采集本地区各县区不同年龄、不同职业的人群血液，用酶联免疫法检测血清中SARS-CoV抗体IgG。结果共检测血清样本1484份，SAILS—CoV抗体IgG总阳性率为0．61％（9／1484），其中0—6岁和13—18岁年龄组SAILS．CoY抗体IgG均为阴性，7—12岁年龄组的阳性率为1．82％（8／439），19岁以上年龄组的阳性率为0．10％（1／1009）。在9份SAILS—CoV抗体IgG阳性的血清样本中，1份为饮食服务业从业人员，阳性率为0．39％（1／257）；其余8份为学生，阳性率为1．70％（8／471）。结论本地区人群SAILS-CoV抗体水平比较低，低龄组0—12岁人群SAILS-CoV抗体IgG阳性率高于高龄组13岁以上组人群的阳性率。提示SAILS-CoV可能存在隐性感染、隐性传播和隐性感染聚集性。     

2010-2017年天津市滨海新区健康人群人肠道病毒71型血清中和抗体动态变化研究

目的:了解天津市滨海新区健康人群人肠道病毒71型（EV-A71）中和抗体动态变化,为手足口病防控方案提供依据。方法:采集2010-2017年天津市滨海新区健康人的血清学标本368份,进行EV-A71中和抗体测定,并对监测结果进行统计学分析。结果:EV-A71中和抗体阳性率为79.35%;不同年份间的抗体阳性率差异有统计学意义（χ2 =6.695,P=0.01）,呈周期性变化;不同年龄组人群的抗体阳性率差异有统计学意义（χ2 =46.531,P<0.01）,5岁及以下年龄组抗体阳性率最低为59.26%;5岁以下各年龄组抗体阳性率无统计学差异（χ2 =1.481,P=0.830）;不同性别间抗体阳性率差异无统计学意义（χ2 =3.480,P=0.062）;以健康人群EV-A71中和抗体阳性率作为应变量,对健康人群的卫生习惯及环境进行Logistic回归分析,发现与没去过医院的相比,半年内去过医院患病风险更大（OR=2.291,95%CI:1.488～4.946）。结论:天津市滨海新区人群EV-A71中和抗体水平较高,易感人群为5岁及以下年龄组儿童,继续加强和完善EV-A71流行监测系统是防控手足口病的重要环节。     

2006年宁夏监测点农村健康人群乙脑免疫水平监测结果分析

目的了解2006年宁夏乙脑监测地区农村健康人群乙脑免疫水平。方法在宁夏2个监测点分别随机抽取6个乡，每乡分为7个年龄组，每个年龄组抽5名调查对象，于2006年5月和11月进行调查并采集血清标本，应用间接法ELISA检测乙脑IgG抗体。结果监测点人群5月份抗体阳性率为16．43％，11月份抗体阳性率为20．71％。不同年龄组人群乙脑抗体阳性率差异有显著性，有免疫史人群的抗体阳性率为13．66％。结论宁夏监测点农村健康人群乙脑抗体阳性率较低，应警惕乙脑流行。要进一步抓好乙脑疫苗免疫接种工作，提高疫苗接种率和接种质量，提高人群免疫水平。     

高邮市2008年正常人群流脑A群、C群抗体监测与分析

目的通过开展流脑流行前期人群抗体水平监测,全面了解和客观评价我市正常人群流脑A群和c群免疫水平,为制定相应的防治措施提供数据资料。方法对高邮市三垛镇0～、1岁～、3岁～、5岁-、7岁-、15岁～、20岁～、40岁以上8个年龄组320名健康人群,每组随机抽取不少于40人,采集血清。采用ELISA法检测人群血清中的A群和C群流脑抗体,并进行统计分析。结果调查的320人中,A群流脑抗体阳性率为74．06％,GMT为1：12．55;C群流脑抗体阳性率62．81％,GMT为1：7．16。3岁以上年龄组的A群、C群流脑抗体阳性率较为满意;3岁以下年龄组流脑A群抗体阳性率较低。结论高邮市三垛镇3岁以上年龄组A群、C群流脑抗体免疫水平较好;0～、1岁～年龄组A群流脑抗体免疫水平不高,经分析其免疫史,与部分儿童初免二针未完成接种有关。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.