ROCK2蛋白在食管鳞状细胞癌中的表达及其临床意义

目的 研究Rho相关的蛋白激酶2(ROCK2)在食管鳞癌组织中的表达水平及其临床意义.方法 收集1990至2001年间在中国医学科学院肿瘤医院接受手术治疗的原发食管癌病例,免疫组织化学方法检测了组织芯片中118例食管鳞癌组织及其配对的癌旁正常食管黏膜中ROCK2的表达水平.将病例按年龄、性别、肿瘤部位、吸烟与否、分化程度、T分期、淋巴结转移及TNM分期进行分组,比较不同组别中ROCK2表达水平的差异.结果 ROCK2在118例食管鳞癌组织中的高表达率为45.76%(54/118).在年龄＜61岁组,ROCK2高表达率为55.74%(34/61),高于年龄＞6l岁组的35.09%(20/57),两者差异具有统计学意义(x2=5.062,P=0.024).高分化病例组中ROCK2高表达率为58.70%(27/46),高于中、低分化病例组的37.50%(27/22),差异具有统计学意义(x2=5.080,P=0.024).结论 ROCK2在食管鳞癌组织中的表达升高与发病年龄及肿瘤的分化程度相关.

Abstract：

Objective To investigate the expression and relationship of Rho-associated protein kinase 2 (ROCK2) and clinical characteristics in esophageal squamous cell carcinoma (ESCC). Methods Immunohistochemistry was performed to assay the expression of ROCK2 in tumor tissues and adjacent normal epithelium from 118 ESCC patients in tissue microarray. The relationship between ROCK2 level and clinicopathologic profiles such as age, gender, location, smoking, differentiation degree, T stage, lymph node metastasis and TNM stage were analyzed. Results The ROCK2 expression was up-regulated in 54 of 118 (45. 76% ) ESCC tissues. The up-regulated expression of ROCK2 was observed in 55.74% (34/61) ESCC tissues of patients under 61 years old. And it was significantly higher than that in 35.09% (20/57) of patients over 61 years old ( x2 = 5.062, P = 0. 024 ). In addition, the rate of up-regulation of ROCK2 was significantly higher in high-grade differentiation group (58. 70%, 27/46 ) than that in moderate-grade and low-grade differentiation group ( 37.50%, 27/72 ) ( x2 = 5.080, P = 0. 024 ). Conclusion The upregulated expression of ROCK2 is correlated with patient age and differentiation grade of ESCC.     

Notch1在食管鳞癌中的表达及其与毛细血管新生的关系

目的 研究Notch1在食管鳞癌中的表达与临床病理特征的关系及其与食管鳞癌新生毛细血管的关系.方法 通过免疫组化方法检测40例食管鳞癌和8例正常食管组织的Notch1和血管内皮生长因子(VEGF)、毛细血管密度(MVD)的表达情况并行相关性分析.结果 Notch1在食管鳞癌组织中表达明显低于正常对照组(P<0.05),在不同的分化程度中有差异(P<0.05),但在肿瘤浸润深度和淋巴结转移与否中无明显差异(P>0.05).VEGF和MVD在食管鳞癌组织中表达明显高于正常对照组(P<0.05),在不同分化程度、浸润深度和淋巴结转移与否中各组间均有显著性差异(P<0.05).相关性分析显示Notch1与VEGF为负相关关系.结论 Notch1在食管鳞癌中可能为抑癌基因;在早中期食管鳞癌中可能是主要影响肿瘤细胞的分化;其的异常低表达可能是引起VEGF和MVD异常高表达的原因之一.Notch信号通路在食管鳞癌新生毛细血管中可能有关键作用.

Abstract：

Objective To observe Notch1 expression in esophageal squamous cell carcinoma (ESCC) and investigate its relation with microvascular angiogenesis in the tumor. Methods Tissue slices of 40 cases ESCC (cancer group) and 8 cases normal esophagus tissues (normal group) were obtained to analyze the expression of Notch 1 and vascular endothelial growth factor (VEGF) using immunohistochemistry and estimate the micro vessel density (MVD) in the tumor. Results Notch 1 expression was significantly lower in the cancer group than in the normal group (P<0.05). In the cancer group, Notch 1 expression was higher in highly differentiated than in poorly differentiated tumors (P<0.05) regardless of tumor infiltration or lymph nodes metastasis (P>0.05). VEGF expression and MVD were significantly higher in cancer group than in normal group, and showed significant differences between tumors with different differentiation degrees, infiltration and lymph node metastasis (P<0.05). Correlation analysis showed that Notchl expression was inversely correlated to VEGF expression. Conclusion Notch1 may be an anti-oncogene in ESCC and affects cell differentiation in early stage of the malignancy. Abnormally low expression of Notchl in ESCC may be one of the upstream factors to induce high expression of VEGF and increased MVD. The Notchl pathway might play a key role in microvascular angiogenesis in ESCC.     

抑癌基因WWOX在食管鳞状细胞癌中的表达及意义

目的 探讨食管鳞状细胞癌(ESCC)组织中WWOX mRNA和蛋白的表达及其意义.方法 应用RT-PCR和免疫组化SP法检测50例ESCC组织、19例癌旁不典型增生组织及50例正常食管黏膜组织中WWOXmRNA和蛋白的表达.结果 WWOX在癌组织、癌旁不典型增生组织及正常黏膜组织中mRNA的相对表达量分别为0.81±0.08、0.84±0.05和0.90±0.05,组间比较差异有统计学意义(F=45.736,P<0.05);不同分化程度、TNM分期、浸润深度及有无淋巴结转移的ESCC组织之间WWOX mRNA相对表达量差异均有统计学意义(P均<0.05).ESCC组织及癌旁不典型增生组织中WWOX蛋白表达均低于正常黏膜组织,蛋白阳性表达率分别为42.0%(21/50)、64.8%(13/19)和84.0%(42/50),组间比较差异有统计学意义(x2=20.002,P<0.01);ESCC组织中WWOX蛋白表达与癌组织的分化程度、TNM分期、浸润深度及有无淋巴结转移密切相关(P<0.05).结论 ESCC组织中WWOX mRNA和蛋白表达均降低,其低表达可能与ESCC发生发展有关.检测WWOX mRNA及蛋白的表达可望成为食管鳞癌早期诊断和预后的分子指标之一. Abstract： Objective To explore the expression of WWOX mRNA and protein inesophageal squamous cell carcinoma (ESCC) tisuss. Methods The expression of WWOX was detected by RTPCR and SP immunohistochemical methods in 50 cases of ESCC tissue, 19 cases of mucosa adjacent to cancer, and 50 cases of normal esophageal mucosa. Results The relative content of WWOX mRNA depressed by turns in cancer tissue, mucosa adjacent to cancer and normal mucosa tissue which were 0.81 ± 0.08,0.84 ± 0.05,0.90 ± 0.05, respectively. And there was significant difference in group comparison (F = 45. 736, P < 0.05). The expression of WWOX mRNA was closely correlated with the tumor grade, TNM grade, infiltrative depth and lymphatic metastasis in ESCC tissue (P < 0.05). The positive rate of WWOX protein expression in cancer tissue and mucosa adjacent to cancer were lower than that of normal mucosa tissue which were 42.0% (21/50), 64.8 % (13/19), 84.0% (42/50) respectively. And there was a significant difference in group comparison(P < 0.05). The expression of WWOX protein was closely correlated with the tumor grade, TNM grade,infiltrative depth and lymphatic metastasis in ESCC tiissue (P <0.05). Conclusions The expression of WWOX mRNA and protein decreased in ESCC tissue, which suggest the WWOX gene may be related to oncogenesis of ESCC tissue. Combined detection of the expression of WWOX mRNA and protein may be used in early diagnosis and prognosis judgement of ESCC tissue.     

中性粒细胞明胶酶相关蛋白及基质金属蛋白酶10在非小细胞肺癌中的表达及临床意义

目的 观察中性粒细胞明胶酶相关蛋白(NGAL)和基质金属蛋白酶10(MMP-10)在非小细胞肺癌(NSCLC)中的表达,探讨其与NSCLC浸润、转移的关系.方法 采用免疫组织化学SP法检测70例非小细胞肺癌组织及30例癌旁肺组织标本中NGAL和MMP-10的表达,并分析它们的表达与相关临床参数的关系.结果 NSCLC组织中NGAL和MMP-10的表达分别为67.1%、70.0%,并与肿瘤直径、TNM分期、淋巴结转移明显相关(P<0.05),而与患者性别、年龄无关.NGAL和MMP-10的表达呈正相关(r=0.376,P=0.001).结论 NSCLC组织中NGAL和MMP-10表达明显增加.NGAL和MMP-10可能在NSCLC的发生、发展中起一定作用. Abstract： Objective To observe the expression of neutrophil gelatinase associated lipocalin (NGAL) and matrix metalloproteinase 10(MMP10) in human non-small cell lung cancer(NSCLC) and to investigate their correlations with the infiltration and metastasis of NSCLC.Methods Immunohistochemistry method was applied to investigate the expression of NGAL and MMP-10 in specimens of 70 NSCLC tissues and 30 paracancerous tissues.The relationship between the expression of NGAL and MMP-10 in the NSCLC tissue and related clinical parameters were analyzed. Results The positive expression rates of NGAL and MMP-10 in NSCLC tissues were 67.1% and 70.0%,respectively. The expression of NGAL and MMP-10 in NSCLC tissues was correlated with the diameter of the mass, the pathological grading, the lymph node metastasis, but not correlated with sex, age and smoking. There was a positive correlation between NGAL and MMP-10 expression in lung squamous cell cancer tissues (r=0.376, P=0.001).Conclusions Higher expression of NGAL and MMP-10 may play an important role in carcinogenesis and development of NSCLC.     

Expression of Pinl and Ki67 in Cervical Cancer and Their Significance

In order to investigate the expression levels of Pinl mRNA and protein in cervical cancer and its association with Ki67 and their clinical significance, amplification of Pinl gene was examined by RT-PCR, and the expression of both Pinl and Ki67 protein was detected by immunohistochemistry in cervical cancer tissues. It was shown that the expression levels of Pinl were higher in cervical cancer than in normal cervical tissues （P〈0.05）. The expression of Pinl protein was increased progressively along with the disease process from normal cervix to CIN and to cervical cancer （P〈0.05）. No significant difference in the Pinl expression was found between disease stages （FIGO）, pathological grades or pelvic lymph node metastasis status （P〉0. 05）. The expression of Pin1 was significantly higher in adenocarcinoma than in squamous carcinoma of the uterine cervix （P〈0.05）. In cervical cancer, the overexpression of Pinl was positively correlated with that of Ki67 （P〈 0. 05）. These results suggested that the overexpression of Pinl was closely related with cancer cell proliferation or progression of cervical cancer and contributed to oncogenesis. Pinl may serve as a potential marker for cervical cancer diagnosis.     

食管鳞癌组织中缺氧诱导因子-1α和6-氧-甲基鸟嘌呤-DNA甲基转移酶蛋白的表达与预后的关系

目的 研究食管鳞状细胞癌(ESCC)中缺氧诱导因子-1α(HIF-1α)、6-氧-甲基鸟嘌呤-DNA甲基转移酶(MGMT)蛋白的表达及其与食管癌病理特征的关系.方法 采用免疫组织化学(SP)法检测58例食管鳞癌组织中HIF-1α、MGMT蛋白的表达,并分析其与临床病理各因素之间的关系.结果 58例患者的ESCC组织中HIF-1α、MGMT蛋白均有表达,阳性表达率分别为51.4%、39.6%,MGMT 蛋白的表达与食管鳞癌的分化程度密切相关(P<0.05),而与性别、年龄、分期及淋巴结转移无关.HIF-1α蛋白的表达与性别、年龄、分期、分化程度以及淋巴结转移均无关(P均>0.05).HIF-1α与MGMT 蛋白表达无明显相关关系(P>0.05).MGMT阳性表达患者生存时间(OS)较阴性组明显延长(P<0.05),而HIF-1α蛋白表达与OS无明显相关(P>0.05).结论 HIF-1α蛋白在食管鳞癌组织中呈高表达,MGMT与其分化程度相关,两者检测对判断预后有重要意义. Abstract： Objective To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) and O~6-methylguanine DNA methytransferase(MGMT) in esophageal squamous cell carcinoma(ESCC) tissue and its relation with the clinicopathalogy features. Methods The expression of HIF-1α, MGMT in 58 cases of ESCC were detected by immnohistochemical staining. Results The positive rates of HIF-1α,MGMT in ESCC tissue were 51.4%,39.6%, respectively. The positive expression rates of HIF-1α and MGMT were not correlated with the degree of sex,age,clinical stage and differentiation(P>0.05), but MGMT expression was correlated with differentiation and overall survival time (P<0.05). The expression of HIF-1α was not correlated with that of MGMT (P>0.05), and HIF-1α was not correlated with overall survival time. Conclusions HIF-1α, MGMT play important roles in esophageal carcinoma,MGMT was correlated with the differentiation of esophageal carcinoma, respectively, which may be used as important indexes to evaluate the prognosis of esophageal carcinoma.     

Expression of PC cell-derived growth factor and vascular endothelial growth factor in esophageal squamous cell carcinoma and their clinicopathologic significance

Background Esophageal carcinoma is a common kind of malignant tumor and about 90% of which is esophageal squamous cell carcinoma （ESCC）, and it has a high incidence in China. In recent years it has been argued that angiogenesis plays a key role in tumor growth and metastasis and it is a complex process influenced by many factors. This study was aimed to investigate the expression of PC cell-derived growth factor （PCDGF or progranulin） and vascular endothelial growth factor （VEGF） in ESCC tissue and their relationship with clinical pathological parameters of ESCC, clarify the role of PCDGF and VEGF in the angiogenesis of ESCC.
Methods The expression of PCDGF and VEGF in 50 surgical specimens from patients with ESCC and 20 with normal esophageal mucosa were detected by immunohistochemistry. The vascular endothelial cells in tumor tissues were labeled by antibody to CD105 for counting microvessel density （MVD）.
Results The expression of PCDGF and VEGF in ESCC was significantly higher than that in normal mucosa. Expression correlated with the depth of tumor invasion, lymph node metastasis, and TNM （classification tumor, nodes, metastasis）. In ESCC, both the expression level of PCDGF and VEGF had a positive correlation with MVD and the expression of PCDGF had a significant correlation with the expression of VEGF.
Conclusions These results show that both of PCDGF and VEGE have higher expression in ESCC, which indicate that they have a close relationship with angiogenesis. They may be involved in tumor growth, infiltration and metastasis through promoting tumor angiogenesis and may be an important index reflecting biological behavior and prognosis of ESCC.     

Vasculogenic mimicry and aberrant expression of HIF-lα/E-cad are associated with worse prognosis of esophageal squamous cell carcinoma

This study aims to find good markers for predicting the prognosis of patients with esophageal squamous cell carcinoma (ESCC). Vasculogenic mimicry (VM) and the expression of hypoxia inducible factor-lα (HIF-lα)/E-cad protein in ESCC were investigated by immunostaining. The association between VM, HIF-lα/E-cad and clinicopathologic characteristics and 5-year-survival rate of patients with ESCC was analyzed. A total of 160 ESCC specimens were involved in this study and 28 specimens of normal esophageal mucosa served as controls. VM channels were identified in 78 (48.75%) of the 160 ESCC specimens and none of the normal esophageal mucosa was found to have VM. The rates of high-expression of HIF-lα and E-cad in ESCC were 43.75% and 38.75%, while the rates in control were 17.86% and 71.43%, respectively (P<0.05 for all). VM and the expression levels of HIF-lα and E-cad were significantly related to lymph node metastasis, serosa infiltration, PTNM staging and 5-year-survival rates of patients with ESCC (P<0.05 for all). VM was positively correlated with HIF-lα but negatively with E-cad, and HIF-lα was negatively correlated with E-cad (P<0.001 for all). The 5-year-survival rate of patients with ESCC was 6.41% (5/78) in VM group and 65% (52/82) in non-VM group, 7.14% (5/70) in high HIF-lα expression group and 57.78% (52/90) in low HIF-lα expression group. Oppositely, the 5-year-survival rate in high E-cad expression group was 80.65% (50/62) and that in low E-cad expression group was 7.37% (7/98) (P<0.05 for all). Cox multifactor regression analysis indicated that lymph node metastasis, PTNM stage, VM and expression levels of HIF-lα and E-cad were independent risk factors of patients with ESCC (P<0.05 for all). Combined detection of VM, HIF-lα and E-cad plays an important role in predicting the invasion, metastasis and prognosis of patients with ESCC.     

Mir-106b表达与食管鳞状细胞癌的关系

目的研究微小RNA106b（mir-106b）在食管鳞状细胞癌组织中的表达情况,并进一步分析其与肿瘤的临床病理学特征及 预后的相关性。方法收集200例2001~2007年中国医学科学院接收的食管鳞状细胞癌患者手术切除获得的肿瘤组织和相对应 的癌旁组织,采用qRT-PCR方法检测mir-106b在组织中的相对表达水平,并通过northern blot进一步验证其表达情况。应用统 计学方法分析mir-106b表达量与肿瘤临床病理学特征及预后的相关性。结果相对于癌旁组织,食管鳞状细胞癌肿瘤组织中 mir-106b的表达水平显著升高,统计学数据显示,mir-106b的表达量与淋巴结转移、肿瘤分期及吸烟有相关性（P<0.05）。并且, mir-106b表达水平较低的患者的生存率（60个月）显著高于mir-106b表达水平较高的患者（37个月,P=0.024）。另外,Cox回归 分析显示,mir-106b的表达量、区域淋巴结转移及吸烟均为是食管鳞状细胞癌患者独立预后因子（P<0.05）。结论mir-106b在 食管鳞状细胞癌患者肿瘤组织中高表达,且与淋巴结转移及预后不良密切相关,可用作ESCC的诊断及预后生物标志物。     

Shh与MMP2在人食管鳞癌组织中的表达及意义

目的探讨音猬因子（Shh）与基质金属蛋白酶2（MMP2）在人食管鳞癌组织中的表达以及与临床病理参数之间的关系。方
法采用免疫组化SP法检测Shh与MMP2在48例人食管鳞癌组织以及44例癌旁组织中的表达情况。结果Shh的阳性表达主
要定位在细胞质和胞核,在食管鳞癌组织中的阳性表达率为75%明显高于癌旁组织（20.45%）,且与患者的TNM分期有关;而
MMP2的阳性表达主要分布在胞质,其在食管鳞癌组织中的阳性表达率为68.75%,明显高于癌旁组织中的22.72%,与淋巴结转
移与否,TNM分期有关,且均具有统计学意义（P<0.01）。48例人食管鳞癌组织中Shh蛋白与MMP2蛋白的表达呈正相关（R=
0.037,P=0.019）。结论Shh蛋白的表达可能参与到食管鳞癌的侵袭与转移。
     

转录因子FoxO1在食管鳞癌组织中的表达及其临床意义

目的检测FoxO1在食管鳞状细胞癌组织中的表达水平,分析其与食管鳞状细胞癌各临床病理因素的关系。方法应用实时荧光定量PCR SYBR Green荧光染料法,检测42例手术切除的食管鳞癌组织及配对的正常食管黏膜组织中FoxO1 mRNA的表达;免疫组化S-P法检测FoxO1蛋白的表达及组织学定位。结果正常食管黏膜组织中FoxO1mRNA及蛋白均呈低表达,而食管鳞癌组织中FoxO1 mRNA及蛋白表达显著升高(P<0.01)。高分化食管鳞癌组织的相对表达量显著高于中低分化食管鳞癌组织(P<0.01)。食管鳞癌组织中FoxO1 mRNA及蛋白表达与患者性别、年龄、肿瘤发生部位、TNM分期及有无淋巴结转移无明显相关性(P>0.05)。结论 FoxO1在正常食管黏膜组织和食管鳞癌组织中的表达存在明显差异,其表达水平与肿瘤分化程度密切相关。     

EIF5A2和Ki67在肝内胆管癌组织中的表达及临床意义

  目的  分析真核翻译起始因子5A2（eukaryotic translation initiation factor 5A2,EIF5A2）和Ki67在肝内胆管癌组织中表达的临床意义及两者的相关性。  方法  收集成都市第七人民医院肝胆外科、昆明医科大学第二附属医院肝胆胰外科2014年9月至2021年12月118例肝内胆管癌患者术后石蜡切片,采用免疫组化的方法检测EIF5A2和Ki67表达,收集临床病理资料,分析EIF5A2、Ki67高表达与不同病理特征的关系及两者的相关性。  结果  肝内胆管癌组织中EIF5A2高表达60例（50.85%）,Ki67高表达81例（68.64%）,EIF5A2高表达与患者年龄、性别、肿瘤直径、肿瘤数量无关（P > 0.05）,与TNM分期和淋巴转移有关（P < 0.05）;肝内胆管癌组织中Ki67高表达与患者年龄、性别、肿瘤数量无关（P > 0.05）,与TNM分期、淋巴转移、肿瘤直径有关（P < 0.05）,EIF5A2与Ki-67的表达呈正相关（r = 0.285,P = 0.002）。  结论  肝内胆管癌中EIF5A2和Ki-67的高表达与高TNM分期和淋巴转移有关,两者表达呈正相关,有助于预判肝内胆管癌患者的临床分期及预后。     

晋东南地区食管鳞癌中 HLA-G 分子的表达及意义

目的：：研究人类白细胞抗原-G(Human Leukocyte Antigen G,HLA-G)在晋东南地区食管鳞状细胞癌(ESCC)中的表达及意义。方法：利用免疫组织化学 SP 法检测66例 ESCC 及25例癌旁组织中 HLA-G 分子的表达。结果：ESCC 中 HLA-G 分子的表达率为62.1%(41/66),明显高出癌旁食管黏膜鳞状上皮表达率32.0%(8/25)(P <0.05)；HLA-G 分子的表达与 ESCC 浸润深度和淋巴结转移关系密切(P <0.05),与患者性别、年龄、肿瘤大小、分化程度和 ESCC 的 TNM 分期无明显相关性(P >0.05)。结论：HLA-G 分子在 ESCC 中表达升高,且与 ESCC 的浸润和转移密切相关,可能在晋东南地区食管鳞癌的发生发展中发挥重要作用。     

程序性死亡配体-1 在食管鳞癌中的表达 及临床意义

目的 分析程序性死亡配体-1（PD-L1）在食管鳞癌中的表达及其临床意义。方法 采用免疫组织 化学染色、Western blot 检测PD-L1 在50 例食管鳞癌患者的癌组织及其相应癌旁组织中的表达,并分析PD-L1 表达与食管鳞癌临床病理学特征及预后的相关性。结果 PD-L1 在食管鳞癌组织中阳性表达率为42.0%（21/50） 高于癌旁组织22.0%（11/50）,差异有统计学意义（P <0.05）。PD-L1 蛋白的表达水平与患者肿瘤浸润深度及临 床分期相关（P <0.05）。Kaplan-Meier 生存分析表明PD-L1 的阳性组较阴性组预后较差,PD-L1 阳性组和阴性 组患者30 个月生存率分别为38.1% 和86.2%,差异有统计学意义（P <0.05）。多变量COX 回归分析结果显示 PD-L1 是食管癌患者的预后因素。结论 PD-L1 蛋白高表达在食管鳞癌的发生发展过程中具有重要作用,可能 成为食管鳞癌预后的评价指标,并为食管鳞癌的治疗提供新的靶点。     

细胞核相关抗原（Ki67）和p53 表达 对非小细胞肺癌患者预后的意义

目的 检测非小细胞肺癌患者肿瘤组织中细胞核相关抗原Ki-67（Ki67）和p53 的表达水平,探 讨其对患者预后（生存率）判断的意义。方法 用免疫组织化学染色半定量检测非小细胞肺癌患者肿瘤组织 中的Ki67 和p53 表达水平。对患者进行随访,分析Ki67 和p53 表达水平与患者生存时间和生存率的关系。 结果 相关性分析结果表明,Ki67 阳性细胞率与非小细胞肺癌患者生存时间具有较弱的相关性（r =-0.200, P =0.030）,p53 阳性细胞率与非小细胞肺癌患者生存时间无相关性（r =0.003,P =0.050）。单因素分析结果提示, Ki67 和p53 共表达以及原发肿瘤局部淋巴结远处转移（TNM）分期对患者生存期有影响,差异有统计学意义 （P =0.000）。当Ki67 和p53 均低表达时,患者的中位生存时间及1 ～ 5 年生存率均高于平均水平。预后影响因 素Cox 回归分析表明,TNM 分期越低,患者中位生存时间越长,死亡风险越低。结论 TNM 分期是影响肺癌 预后的指标,TNM 分期越高,患者死亡风险越大。Ki67 和p53 均低表达时,中位生存时间及1 ～ 5 年生存率 均高于平均水平,提示Ki67 和p53 联合检测可为非小细胞肺癌早期诊断及预后判断提供辅助依据。     

C-Jun结合蛋白在肝癌细胞组织中的高表达及其意义

目的 探讨C-Jun结合蛋白(JAB1)在肝癌细胞(HCC)组织中的表达及临床病理意义.方法 收集76例HCC及癌旁组织,10例肝血管瘤旁肝组织的临床病理档案资料,采用免疫组织化学方法检测JAB1蛋白质在这些组织中的表达,并与增殖细胞核抗原(Ki67)的表达相比较.应用统计学方法对结果进行分析.结果 HCC组织中JAB1蛋白质表达的阳性率(68.85%)显著高于癌旁组织(38.72%)(P＜0.001)和血管瘤旁肝组织(34.36%)(P＜0.001).JAB1的表达与肝癌组织分化程度,血清AFP值及转移有关(P=0.000,P=0.015及P=0.000),但与患者的年龄、性别、肿瘤组织学类型、肿瘤大小等无关.HCC组织中Ki67表达的阳性率为41.45%,明显高于癌旁组织2.11%(P＜0.1301)和血管瘤旁肝组织2.01%(P＜0.001).相关性分析表明HCC组织中JAB1蛋白质表达与Ki67表达呈正相关(r=0.6192,P＜0.001).结论 JAB1在HCC组织中呈高表达,且显著高于非癌肝组织,与HCC的发生发展有关;JAB1可作为HCC细胞增生程度的潜在临床病理标记.