川楝子油对雄性大鼠的抗生育作用

目的：研究川楝子油对雄性大鼠的抗生育作用。方法：实验组ＳＤ大鼠２０只，川楝子油两侧附睾尾部注射，１００μｌ／侧。１０天后，将上述大鼠分别与有生育力的雌鼠进行交配研究、ＦＣＭ检查、组织学研究、睾丸酮测定。结果：用药组及对照组大鼠生育率分别为１３．３％和９０％。用药组５周时组织学检查附睾及输精管，均无梗阻改变；睾丸形态正常；附睾出现了轻度炎症。６周时睾丸曲细精管口径缩小。ＦＣＭ表明川楝子油可抑制睾丸生     

十一酸睾丸素与达那唑合用对雄性大鼠的抗生育作用

给雄性大鼠肌注十一酸睾丸素,同时用达那唑灌胃,观察睾丸及附睾的形态与功能变化,以评价其抗生育作用。给药35d.睾丸及曲细精管径缩小,附睾尾精子活力显著降低,睾丸内睾丸素及附睾内蛋白质与磷脂含量均明显下降;给药75d,生精过程受抑,严重少精或无精,交配雌鼠生育率0％。停药100d后,上述变化均恢复正常,附睾管腔精子充盈,生育率100％。表明TU与DZ合用具有可逆性抗生育作用,其作用机理主要是由于降低睾丸内睾丸素水平,从而使精子生成及存活受抑。     

乙胺嘧啶对雄性大鼠抗生育作用的实验研究

目的 :研究乙胺嘧啶的抗生育作用及其可逆性。方法 :通过给SD雄性大鼠灌服不同剂量乙胺嘧啶 5 0d后 ,观察其睾丸和附睾质量及组织学形态、附睾尾部精子活率和畸形率、血清睾酮含量、雄鼠的致孕率变化。结果 :15 0mg·(kg·d) - 1 组变化最明显 ,睾丸质量和脏器系数下降 ,睾丸曲细精管生精细胞排列疏松和结构紊乱 ,附睾上皮细胞肿胀和纤毛脱落 ,致孕率显著下降 ,实验各组附睾尾部精子存活率和畸形率均有下降 ,但血清睾酮含量没有显著变化。结论 :乙胺嘧啶对睾丸生精过程和附睾上皮功能有影响 ,但不影响血清睾酮的分泌     

川楝子油体外杀精子研究

采用Ｓａｎｄｅｒ－ｃｒａｍｅｒ法评价了川楝子油体外杀精子效果。川楝子油在２０ｓ内可使精子丧失活力，而川楝子油－环己酮复合物对精子的影响随其浓度增加而增强。精子复活试验表明对精子的上述影响为不可恢复性的。     

黑木耳多糖对小鼠的抗生育作用

前已报道黑木耳多糖具有多种生物活性和药理作用,如促进核酸、蛋白质生物合成和免疫功能,抗衰老、防治心血管疾病和细胞保护作用等,本文报道其抗生育作用。     

穿心莲抗生育作用的实验研究

目的研究穿心莲的抗生育作用。方法采用体内外给药的方法,观察穿心莲对大鼠精子、胚胎及小鼠精子的影响。结果穿心莲可使胚胎发育异常,精子数量和存活率降低。结论穿心莲对精子发育有明显影响。     

九里香的抗生育作用

九里香有抗炎、抗生育和治疗跌打损伤等功效,福建省莆田县民间验方用于引产。本文对九里香的抗生育作用进行了初步研究。观察了九里香不同药用部分、不同给药途径对小鼠各妊娠阶段的抗生育作用,及其对小鼠在体和离体子宫及前列腺素E_2合用的抗早孕作     

Evaluation of Antifertility Effect and Recovery of the Seed Oil Constituents of Iranian Species of Melia Azadrach L. in Male Rats

Objective To evaluate the fertility effects of seed extract of Melia azadarach L. treatment effects on fertility indices in male Wistar rats.
Methods The seed oil extract had been prepared according to conventional methods. The rats were randomly divided into three study groups. Groups A and B received graded doses of 50 mg/kg and 100 mg/kg body weight of the extract oil, respectively on daily basis for 60 d. Animals in control group received I ml of maize oil. At the end of 60 d and 3 months treatment period, 6 animals per group were randomly selected and fertility was evaluated with mating test. GSI （gonadosomatic index） sperm motility, sperm viability, ESR （epididymal sperm reserves）, DSP（daily sperm production） and testosterone concentration were also assessed.
Results In the first stage, a significant reduction infertility indices especially in higher dose was observed compared with the control. During the next stage, the significant increase in fertility indices are the indication of reasonable recovery and reversibility of extract activity.
Conclusion The seed oil of Melia azadarach L. has antifertility activity, but its effects is reversible.     

烹调油烟对大鼠精子的形态学影响

雄性ｗｉｓｔａｒ大鼠吸入烹调油烟后，通过对其精子形态学进行观察，发现吸入烹调油烟后的雄性大鼠精子数量减少，活动率降低，活动度变差，畸形率增加，且畸形精子类型多为无钩型和香蕉型。提示：吸入烹调油烟对大鼠精子具有毒作用，可能是通过遗传损伤、直接损伤作用等多种途径干扰生精细胞的分化与代谢，从而导致精子形态学改变。     

Antifertility Effects of Orally Administration of Low Dose Gossypol Acetic Acid Combined with Methyltestosterone Plus Ethinyl Estradiol on Male Rat

Objective To investigate the feasibility and optimal regimen of orally administration of low dose gossypol acetic acid （GA） combined with methyltestosterone （MT） plus ethinyl estradiol （EE） for contraception in males.
Methods Wistar male rats were randomly assigned into four groups, 20 in each group. Animals in group A or B were administered daily with 1% methyl cellulose or GA （12 mg/kg） suspended in 1% methyl cellulose, respectively. Rats in group C or D took firstly GA 12 mg/kg＋MT 20 mg/kg＋EE 0.1 mg/kg or MT 20 mg/kg＋EE 0.1 mg/kg, in a
suspension with 1% methyl cellulose, via gastric intubation. After the infertilities were initiated（6 weeks for group C, 8 weeks for group D）, GA was served alone while MT＋EE were withdrawn in rats of groups C and D. The treatment was ceased after 18 weeks and some males from group C were permitted to recover. Fertility testing, 10 males per group, was served for determining infertility or restoration of fertility in treated rats. Examinations of histology and biochemistry in treated rats were used to examine the morphologic influences on sperm, testis, epididymides and viscera, and biochemical changes in blood. The growth and development of F1 generation of the rats would also be tested in a series of behavioral tests.
Results Ten rats from group C were infertile at week 6 after treatment, and the fulfilled infertility was maintained with low-dose GA （12 mg/kg） only daily. Six weeks after cessation of treatment, all of treated males recovered their fertility. However, 8 of 10 rats from group D were in sterility at 6th week of treatment and all at 8th week of treatment, but the infertility could not be kept with the similar dose GA alone later on. Moreover, no adverse effects were found in our present experiments.
Conclusion Administration of oral low dose GA combined with MT and EE as loading dose could successfully induce infertility in short term, whereafter the efficacy could completely be maintained by similar low dose of GA alone for lon     

抗生育药对大鼠培养颗粒细胞纤维蛋白溶酶原激活因子和孕酮分泌的影响

采用纤维蛋白铺盖和放射免疫方法研究了米非司酮(RU486)和18-甲基炔诺酮(NG)对大鼠培养颗粒细胞纤维蛋白溶酶原激活因子和孕酮分泌的作用,结果显示RU486和NG能明显抑制培养细胞孕酮分泌,同时可拮抗入绒毛膜促性腺激素促进孕酮分泌的作用。但它们对颗粒细胞和卵母细胞分泌纤维蛋白溶酶原激活因子(PA)的作用没有明显的作用,提示RU486和NG发挥抗生育作用主要是通过影响内分泌,而不是通过影响PA分泌过程而实现的。     

雷公藤总甙对大鼠生精作用及主要脏器的影响

给大鼠每日喂服雷公藤总甙(TⅡ)10mg/kg 7、13周和20mg/kg 4、10周,结果其精子发生及曲细精管晚期精子细胞碱性核蛋白的替代和转换均受到明显抑制;附睾尾精子数目明显减少,残存精子畸变,头部膨大,头颈断裂,颈部断端高度卷曲;精子线粒体酶反应颗粒减少。心、肝及肾多种酶活性与对照组比较无明显差别,但3种组织的SDH及肾小管中的ACP显著增强,近曲小管刷状缘的ALP活性轻微减弱,提示3种主要器官的轻微改变是机体对TⅡ毒性的一种适应性反应。     

高效液相色谱法测定金铃子滴丸中延胡索乙素的含量

目的建立测定金铃子滴丸中延胡索乙素含量的高效液相色谱法。方法采用HPLC法测定延胡索乙素含量,色谱柱为依利特C18(4.6mm×200mm,5μm);流动相为甲醇∶0.1%磷酸溶液(三乙胺调pH值至6.0)(55∶45);检测波长为280nm,流速为1.0mL·min-1,柱温为30℃,进样量10μL。结果延胡索乙素在0.01～0.20mg.mL-1范围内质量浓度与峰面积呈良好的线性关系,相关系数r=0.9999(n=7);平均回收率为100.25%,RSD为1.71%(n=6);重复性实验的相对标准偏差RSD为1.87%(n=6)。结论该测定方法重复性好、灵敏度高并且简便、准确,可作为金铃子滴丸中延胡索乙素含量的测定方法。     

雷公藤单体（T4）与棉酚抗生育机理及毒性作用的比较

喂服雷公藤单体(T_4)0.05mg/kg/d6周后,大鼠生精作用及晚期精子细胞碱性核蛋白转换与替代受到抑制程度均较喂服棉酚组明显。实验组附睾上皮腔缘部γ-GT,ALP出现成簇的阳性沉淀。附睾尾部精子头尾断裂,断端卷曲,尾部纵行粗纤维脱位,线粒体LDH,LDH-X,MDH受到抑制。T_4对肾脏、肝脏各种酶虽有轻度影响但没有引起酶活性明显的变化。T_4对内脏的影响较棉酚轻,但致雄性不育的效果比较明显。本研究提示T_4是一种有发展前途的男性抗生育药物。     

大叶牛奶菜抗生育成分的研究

经过药物筛选发现大叶牛奶菜对SD大鼠具有抗生育作用,从其甲醇提取物中分得大叶牛奶菜甙甲(Marsdckoiside A,1)及大叶牛奶菜甙乙(Marsdekoiside B,2),根据波谱分析和水解产物与标准品对照确定了它们的结构,证明二者都是新化合物。动物实验甲具有抗生育活性。     

烹调油烟对大鼠体内VitC及过氧化脂质的影响

本文通过大鼠连续吸入烹调油烟染毒，观察不同时间烹调油烟对大鼠体内血液中ＶｉｔＣ、ＭＤＡ及肺组织ＭＤＡ含量的变化。结果表明：烹调油烟可使ＶｉｔＣ含量降低、且随染毒时间延长血清中ＶｉｔＣ含量明显降低，与阴性对照比较ＶｉｔＣ含量的降低具有高度显著性（Ｐ＜０．０１）。烹调油烟染毒组ＶｉｔＣ含量降低的同时，可见血清及肺组织中ＭＤＡ含量增加，两者ＭＤＡ含量增加与阴性对照组比较其差别均具有高度显著性（Ｐ＜０．０１）。