促性腺激素及雌二醇对小鼠卵母细胞体外成熟的影响

目的:探讨取卵前给予孕马血清(PMSG)或人绒毛膜促性腺激素(HCG)以及在培养液中添加不同浓度雌二醇对小鼠未成熟卵母细胞体外成熟(IVM)及胚胎发育的影响。方法:将雌性小鼠随机分成5组,即24 h PMSG组(取卵前24 h给小鼠腹腔注射PMSG)、48 h PMSG组(取卵前48 h给小鼠腹腔注射PMSG)1、6 h HCG组(取卵前16 h给小鼠腹腔注射HCG)、PMSG+HCG组(给小鼠腹腔注射PMSG 48 h后腹腔注射HCG 16 h取卵)和未用药组。取未成熟卵丘-卵母细胞复合体(COCs)置入含不同浓度E2(0,0.5,1.0,2.0 mg/L)培养液中培养24 h,并行卵细胞胞质内单精子注射(ICSI)及胚胎培养。比较各组之间卵母细胞成熟率、受精率和卵裂率。结果:①24 h PMSG组和48 h PMSG组COCs的卵母细胞成熟率明显高于未用药组或16 hHCG组,差异有统计学意义(P<0.05)。PMSG+HCG组的受精率和卵裂率明显高于其他4组(P<0.01)。②与对照组比较,1.0 mg/L E2组和1.5 mg/L E2组中COCs的卵母细胞受精率和卵裂率明显增高(P<0.05)。各浓度17β-E2组间以及与对照组比较DO的成熟率和受精率无显著差异(P>0.05),但极少数受精卵发生卵裂。结论:①取卵前给予小鼠PMSG或者联合给与HCG均可促进卵母细胞体外成熟,但不能提高其早期胚胎发育能力;取卵前给予小鼠HCG不能提高卵母细胞体外成熟及早期胚胎发育能力。②在培养液中添加一定浓度E2可促进未成熟卵母细胞胞质成熟,但不能促进核成熟。     

不同成熟时期山羊卵母细胞冷冻保存研究

目的比较山羊卵母细胞在不同成熟时期解冻后体外成熟、体外受精及胚胎发育能力。方法收集山羊生殖泡（GV）期未成熟卵和成熟卵,分5组行开放式拉细麦管（OPS）法玻璃化冷冻,Ⅰ组：直接冷冻GV期卵;Ⅱ组：GV期卵在体外培养（IVC）8h后冷冻;Ⅲ组：GV期卵在IVC16h后冷冻;Ⅳ组：GV期卵体外培养成熟（IVM）后冷冻;Ⅴ组：直接冷冻体内成熟卵。解冻后比较各组卵受精率、卵裂率及8细胞胚胎形成率。结果Ⅰ组（GV期卵）冷冻解冻后存活率达56.8%,显著高于Ⅱ组（33.3%）、Ⅲ组（31.6%）、Ⅳ组（28.9%）及Ⅴ组（30.4%）,差异有高度统计学意义（均P〈0.01）。各组体外受精率、卵裂率及8细胞胚胎形成率比较,差异无统计学意义（P〉0.05）。结论采用OPS法玻璃化冷冻山羊GV期卵较冷冻其他成熟时期卵效果好。     

不同体外受精周期所获生殖泡期卵母细胞体外 成熟后结果比较

 【目的】 研究对比体外受精-单精子卵胞浆内注射(IVF-ICSI)周期所获生殖泡期(GV)不成熟卵母细胞以及体外成熟(IVM)周期所获GV期卵母细胞体外培养成熟后的胚胎发育情况&#65377;【方法】 163个IVF-ICSI周期中所获987个成熟卵为Ⅰ组,所获GV期卵进行体外培养后成熟的132个卵为Ⅱ组, 另有37个IVM周期中所获GV期卵体外培养后成熟的235个卵为Ⅲ组&#65377;对3组均进行ICSI,并对其受精率&#65380;卵裂率及胚胎发育进行比较&#65377;【结果】 Ⅰ组的受精率&#65380;卵裂率以及优质胚胎率均显著高于Ⅱ组及Ⅲ组(分别为84.9%,98.1%和61.6%;72.0%,90.5%和22.1%; 75.3%,94.4%和25.1%)&#65377;Ⅰ组的胚胎卵裂球数目及胚胎形态评分均显著优于Ⅱ组及Ⅲ组(P < 0.05);Ⅱ组与Ⅲ组相比差异无统计学意义&#65377; 【结论】 体内成熟卵形成的胚胎形态好于生殖泡期卵体外培养成熟者,IVF-ICSI周期的生殖泡期不成熟卵形成的胚胎形态学上与IVM周期的类似&#65377;     

三种不同来源人未成熟卵母细胞的体外成熟

目的：探讨三种不同来源的人未成熟卵母细胞体外成熟（IVM）的差异。方法：共获得未成熟卵母细胞1055枚,包括卵丘复合物（OCC）613枚和裸卵（DO）442枚。其中OCC分为自然周期组、孕晚期组和IVM纽;DO分为自然周期组、孕晚期组和超促排卵纽。所有未成熟卵母细胞在IVM培养体系体外培养成熟后,卵母细胞胞浆内单精子注射（ICSI）受精序贯培养,除IVM组行第2或第3天胚胎移植外,其余均培养至囊胚阶段。分别比较OCC和DO各组未成熟卵母细胞的成熟率、成熟卵母细胞的受精率、卵裂率;自然周期组和孕晚期组的囊胚形成率。结果：OCC：自然周期组、孕晚期组及IVM组的未成熟卵母细胞体外成熟率分别为79．5％、74．3％和82．2％,孕晚期组与IVM周期组相比具有统计学差异;3组成熟卵母细胞受精率、卵裂率及自然周期组和孕晚期组的囊胚形成率相比无统计学差异,IVM组的移植周期妊娠率为20％。DO：自然周期组、孕晚期组及超促排卵组未成熟卵母细胞体外成熟率超促排卵组最高（86．0％）,明显高于自然周期组（74．5％）和孕晚期组（72．7％）,（P〈0．05和〈0．01）。3组未成熟卵母细胞的成熟率、成熟卵母细胞的受精率、卵裂率、囊胚形成率相比未见统计学差异。结论：孕晚期来源的未成熟卵母细胞在体外同样具有发育潜能;孕晚期的卵母细胞可作为一种供卵来源。     

不成熟卵母细胞的体外成熟培养及玻璃化冷冻

目的 比较显微授精治疗周期中剩余的生发泡(GV)期不成熟卵母细胞的体外成熟培养及玻璃化冷冻效率.方法 将显微授精(ICSI)周期剩余的GV期卵母细胞随机分为3组:①在GV期玻璃化冷冻,解冻后进行体外成熟培养;②体外成熟培养至MII期后行玻璃化冷冻;③对照组:直接进行体外成熟培养(IVM).观察体外培养成熟卵母细胞及不同成熟阶段卵母细胞冷冻后的受精和发育潜力.结果 对照组的成熟率为71.6%(53/74),其中16枚行显微授精(ICSI),受精率为87.5%(14/16),卵裂率为85.7%(12/14),囊胚形成率为14.3%(1/7);组1与组2的存活率分别为97.5%(39/40)和87.5%(35/40),差异没有显著性;但是组1的成熟率28.2%(11/39)显著低于(P<0.01)对照组的成熟率71.6%(53/74);组2的受精率74.1%(20/27)与对照组的受精率87.5%(14/16)相比,差异没有显著性.结论 ICSI周期剩余的GV期卵母细胞能在体外培养成熟并具有良好的发育潜力,将GV期卵母细胞体外成熟培养至MⅡ期更适合于玻璃化冷冻保存.     

表皮生长因子促进小鼠未成熟卵母细胞体外成熟的实验研究

目的 了解表皮生长因子(epidermal growth factor,EGF)对小鼠未成熟卵母细胞体外成熟、受精及胚胎发育的影响.方法 将生殖泡(germinal vesicle,GV)期卵母细胞分别在EGF浓度为0(对照)、0.5、1、2.5、5、10 ng/ml的培养液中进行体外培养,观察生殖泡破裂(germinal vesicle breakdown,GVBD)和第一极体(the first polar body,PB1)排出的情况.将达到MⅡ期的卵母细胞进行体外受精,观察原核和胚胎发育情况.结果 EGF浓度为1、2.5、5、10 ng/ml组GVBD发生率高于对照组;EGF浓度2.5、5、10 ng/ml组PB1排出率高于对照组,其中EGF浓度5 ng/ml组的GVBD、PB1发生率最高(P＜0.01).卵母细胞在1、2.5、5、10 ng/ml EGF培养浓度下的退化率低于对照组(P＜0.05,P＜0.01).卵母细胞的体外受精率、2-细胞和4-细胞胚胎的发生率在EGF浓度为5 ng/ml时明显高于对照组(P＜0.01).结论 EGF可促进GV期卵母细胞的成熟,对其受精能力和胚胎发展也有促进作用,也可能有抑制卵母细胞凋亡的作用,而EGF以上作用与剂量有关.     

两种培养基对卵母细胞体外成熟效果比较

目的：观察两种培养基在未成熟卵母细胞体外培养时,对卵子成熟及授精后胚胎发育的影响.方法：144枚未成熟卵母细胞随机分为两组行体外成熟培养,组1为囊胚培养基,共培养80枚卵子;组2为人输卵管液培养基,共培养64枚卵子培养,两种培养基除基础液不同,其余组分及含量均相同,比较两组卵子的体外成熟、受精及胚胎发育情况.结果：组1卵子成熟率（86.25％）明显高于组2（60.94％）,差异有统计学意义（P＜0.05）;两组卵子的受精率、卵裂率及优胚率无明显差异（P＞0.05）,但组1有优于组2的趋势.结论：两种培养基均可将未成熟卵母细胞培养成熟,但囊胚培养基更优于人输卵管液培养基,且方便配制,更适用于临床.     

玻璃化冷冻与慢速程序冷冻法保存人MⅠ期卵母细胞的效果比较

目的:探讨玻璃化冷冻与慢速程序冷冻法对人未成熟卵母细胞的存活及胚胎发育潜能的影响。方法:体外受精-胚胎移植中获得的251个MⅠ期卵母细胞随机分3组,第1组94个先玻璃化冷冻保存,第2组63个先慢速程序冷冻保存,两组冻融后的未成熟卵母细胞再进行IVM,用ICSI技术对成熟卵母细胞完成授精,并进行体外胚胎培养。第3组94个MⅠ期卵母细胞先进行IVM,再进行玻璃化冷冻保存,与第1组先玻璃化冷冻保存再IVM的顺序不同,冻融后同样用ICSI技术对获得的成熟卵母细胞完成授精,并进行体外胚胎培养。结果:玻璃化冻融组复苏存活率、体外成熟率、受精率、及卵裂率均高于慢速程序冻融组(P均<0.05);先玻璃化冷冻再IVM与先IVM再玻璃化冷冻的复苏存活率、体外成熟率、可用卵比率、受精率及卵裂率无统计学意义(P均>0.05)。结论:玻璃化冻融人未成熟卵母细胞能获得更好的冷冻保存效果;玻璃化冷冻与IVM的顺序不影响未成熟卵母细胞的利用。     

冷冻对人类成熟及未成熟卵发展潜能的影响

目的：探索冷冻卵子的成熟时期，方法：将153个生发泡期(GV期)人类卵子分为3组，对照组：体外培养至成熟(MⅡ期)，行单精子胞浆内注射(ICSI)，MⅡ期冷冻组：体外成熟至MⅡ，然后将MⅡ期卵子进行冷冻，解冻后将存活的卵子行ICSI；GV期冷冻组：将GV期卵子进行冷冻，解冻后体外培养至MⅡ，然后再行ICSI．用慢速冷冻快速解冻的方法对成熟的和未成熟的卵子进行冷冻和解冻，观察体外成熟情况、解冻后的存活情况、受精情况及胚胎发育情况，结果：体外成熟率：GV期冷冻组51.2％，对照组为70．0％，有显差异；受精率：3组分别为68、0％、33、3%、42.1％，有显差异．对照组的发展潜能要显优于试验组；GV期冷冻组的存活率、受精率优于MⅡ期冷冻组。结论：冷冻会对卵子的发育潜能有1定的损伤，在GV期进行冷冻可降低这种伤害。     

17β-雌二醇对小鼠卵母细胞体外成熟及胚胎发育的影响

目的:探讨培养液中添加17β-雌二醇(17β-E2)对小鼠生殖泡期(GV)带卵丘细胞卵母细胞体外成熟、受精及其胚胎发育的影响.方法:小鼠生殖泡期卵母细胞随机分为2组:对照组(n=61,TCM199基础培养液加体积分数10%胎牛血清加75 U/L 卵泡刺激素加0.5 kU/L 人绒毛膜促性腺激素加0.05 g/L青霉素加0.075 g/L链霉素)和17β-E2组(n=66,对照组液加1 mg/L 17β-E2),培养后对成熟的卵母细胞行胞浆内单精子显微注射,观察受精及胚胎发育情况.结果:17β-E2组卵母细胞的桑葚胚、囊胚形成率均高于对照组(P均<0.05).结论:成熟培养液中加入17β-E2有助于小鼠未成熟有卵丘细胞卵母细胞的体外成熟及受精后胚胎发育.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.