AAALAC执行主席来京讲学

对4－12月龄巴马小型猪（♂20,♀30）的24项血液生理指标进行测定和分析。结果表明,巴马小型猪血液生理指标性别差异不显著;但多数项目不同月龄间差异极显著（P＞0.01）或显著（P＜0.05）,并有近半数项目随月龄的增长呈减少或增加的趋势,其余多无明显的变化规律。多数项目和普通猪相近似;血液生理指标中有14个项目和人类相近似,说明巴马小型猪和人类的生理特性有一定的相似性。     

四个品种小型猪血液学指标测定及比较

目的 分析比较四个品种小型猪的血液学生物学特性.方法 分别选取上海地区饲养的巴马小型猪、贵州小型猪和版纳小型猪及陆川小型猪,每个品种雌雄各20头,3～6月龄,测量其血液生理、血清生化与血凝指标,然后进行单因素方差分析,并进行数据比较.结果 四个品种的小型猪血液生理14个指标差异有统计学意义(P＜0.05),血清生化12个指标组间差异有统计学意义(P＜0.05),血凝4个指标差异都有统计学意义(P＜0.05).分别对各品种群体数据进行比较分析,巴马小型猪群体雌雄间血液常规、血清生化和血凝指标差异显著(P＜0.05)的指标分别有6项、4项和3项,而贵州小型猪、版纳小型猪和陆川小型猪雌雄间差异指标较少或差异不显著.四品种小型猪相同性别间比较,也有较多指标差异显著.结论 测定和比较的巴马小型猪、贵州小型猪、版纳小型猪和陆川小型猪在血液学、血清生化和血凝等方面数据,为应用和选择小型猪开展相关生物医学和药物研究提供了基础数据.     

广州地区三种小型猪血液生理生化指标的比较

目的 比较广州地区西藏小型猪、五指山小型猪和巴马小型猪血液生理生化指标的差异.方法 全自动血液细胞和生化分析仪测定24个血液生理生化指标.结果 24个指标中,血红蛋白、白细胞、单核细胞、血细胞比容、葡萄糖指标的测定差异显著;淋巴细胞、中性粒细胞、平均红细胞容积、平均红细胞血红蛋白、血清总蛋白、血清清蛋白、血尿素氮、血肌酐指标的测定差异极显著.其中血红蛋白、血细胞比容、平均红细胞容积、平均红细胞血红蛋白、血清总蛋白、血清清蛋白、血尿素氮、血肌酐指标以西藏小型猪最高,白细胞、淋巴细胞指标以五指山小型猪最高,而中性粒细胞、单核细胞、葡萄糖指标以巴马小型猪最高.结论 三种小型猪血液生理生化指标的比较,为建立小型猪生物学特性数据库提供了理论依据.     

体细胞克隆巴马小型猪部分生理生化指标的初步测定

目的探讨体细胞克隆巴马小型猪的生理生化指标特性。方法采用全自动血球计数仪和全自动生化仪,对体细胞克隆巴马小型猪血液的部分生理和生化指标进行测定和分析。结果体细胞克隆巴马小型猪生理生化指标和同期普通巴马小型猪没有显著差异,与人类大部分指标接近。结论体细胞克隆巴马小型猪和人类的生理生化特性有一定的相似性。     

西藏小型猪与高原藏猪的血液生理生化值的比较

目的 比较西藏小型猪与高原藏猪的血液生理、生化指标的差异.方法 采用全自动血液细胞和生化分析仪测定21个血液生理生化指标.结果 藏猪与西藏小型猪之间,红细胞、白细胞、血小板计数、总胆固醇、血清甘油三酯指标的测定差异有显著性,谷草转氨酶、血清总蛋白、血清清蛋白、葡萄糖、肌酸激酶指标的测定差异极显著.其中红细胞、白细胞、谷草转氨酶、肌酸激酶的值藏猪高于西藏小型猪,而血小板计数、血清总蛋白、血清清蛋白、葡萄糖、总胆固醇、血清甘油三酯的值藏猪低于西藏小型猪.结论 不同的生活环境、气候条件和营养水平会对动物的血液生理、生化值的测定造成一定的影响.     

<中国实验动物学报>稿约

目的 对2～12月龄封闭群五指山小型猪的部分血液生理和生化指标进行测定和分析.方法 常规方法测定五指山小型猪血液的19项生理和12项生化指标,统计分析各指标间的性别差异.并与近交系五指山小型猪以及其他小型猪的同类指标进行对比分析.结果 封闭群五指山小型猪绝大多数血液生理、生化指标性别间差异不显著,仅生理指标中的粒细胞分类计数和生化指标中的总胆固醇差异显著.同近交系五指山小型猪同类指标比较,生理指标中血红蛋白和血小板相关指标存在差异,生化指标中有6项差异显著.同其他种类小型猪同类指标差异较与近交系五指山小型猪间差异明显.结论 封闭群五指山小型猪血液生理、生化指标稳定,许多生理生化指标接近人类,可能逐步替代犬、猴用于药物非临床安全评价和生物医学研究.     

不同性别、年龄Wistar大鼠常规血液生化值测定

目的研究性别和年龄因素对SPF级近交系Wistar大鼠血液生化值的影响。方法用ECHO全自动血液生化分析仪测定SPF级近交系Wistar大鼠血液生化值。结果各周龄大鼠雌雄间各项血液生化指标比较结果：幼龄期3周龄动物的总胆固醇(TC)雌雄间差异显著(P＜0．05)；生长期6周龄动物白蛋白(ALB)、总蛋白TP雌雄间差异极其显著(P＜0．01)；生长期6周龄大鼠的天冬氨酸转氨酶(AST)、甘油三脂(TG)、TC等指标雌雄间有显著差异(P＜0．05)。成年期13周龄动物丙氨酸转氨酶(ALT)、ALB、甘油三脂(TG)指标雌雄间差异极其显著(P＜0．01)；老年期52周龄大鼠的尿素氮(BUN)、TP、TG、ALT等指标雌雄间差异极其显著(P＜0．01)；老年期(52周龄)大鼠的AST雌雄间有显著差异(P＜0．05)。同性别各周龄大鼠的一些血液生化指标也存在明显差异。结论年龄、性别因素对SPF级近交系Wistar大鼠的血液生化值有极其显著的影响。     

长白仔猪血常规及生化指标正常值探讨

目的探讨2-3月龄长白仔猪的血常规及生化指标的正常值，为长白仔猪用于实验研究提供实验依据。方法采用实验室评价方法对40头长白仔猪的主要血常规和生化指标进行检测，并与巴马小型猪的参考值进行对比。结果长白仔猪的血常规及生化指标无性别差异（P〉0．05），长白仔猪的WBC、ALT、Ca^2＋的平均值与巴马小型猪相似，两者之间差异无显著性（P〉0．05），其RBC、HGB、HCT、PLT、TP、Alb、BUN、AMY的平均值均低于巴马小型猪的相应值，而AST、TBIL、DBIL、Crea、CK-MB明显高于巴马小型猪，具有显著性差异（P〈0．01）。结论长白仔猪有其独特的生理生化特性。     

融水小型猪F1代的血常规、血生化与电解质指标

目的 测定融水小型猪（Rongshui miniature pig,RMP）F1代血常规、血生化与电解质指标。方法 选取4月龄F1代RMP 85头,雌性43头、雄性42头;12月龄36头,雌雄各半。颈静脉采血,分析血常规、血生化与电解质等22项指标。结果 融水小型猪相同月龄时,两性别的血常规指标、血生化和电解质的大部分指标差异不大,但同性别在不同月龄之间的部分指标差异明显。12月龄比4月龄融水小型猪,血常规指标中的白细胞数、血小板数下降,血红蛋白含量升高（P< 0. 05）,而红细胞数变化不大（P> 0. 05）;血清ALT、AST、ALP、CK(雄性)、LDH（雄性）A/G、BUN、GLU(雌性)、CHOL（雄性）和K+下降（P< 0.05）;血清总蛋白、TBIL、CR、Ca2+升高（P< 0.05）,而血清CHOL（雌性）、TG,HDL-C,LDL-C变化不大。有19项生化、电解质指标在或接近人类正常值范围,占86.4％（19／22）。结论 融水小型猪的部分血常规、大部分血生化与电解质指标与人类正常值接近。     

三品种实验用小型猪繁殖性能测定

目的 选取饲养于上海地区的巴马小型猪、贵州小型猪和版纳小型猪,分别测定三品种母猪和公猪的繁殖性能.方法 采用场内结合实验室测定的方法,分别测定初情年龄、初配年龄等母猪繁殖性能指标及性行为年龄、初配年龄等公猪繁殖性能指标.结果 用单因素方差分析比较.结果对比巴马小型猪、贵州小型猪和版纳小型猪的母猪繁殖性能10个指标,贵州小型猪和版纳小型猪的初生窝重和断奶成活率存在显著差异(P＜0.05).分析三品种的公猪繁殖性能,群体间性行为年龄存在显著差异(P＜0.05);三品种初配年龄比较接近;比较初配年龄和初配体质量,表明三品种的小型猪在相同初配年龄时,巴马小型猪个体偏小,版纳小型猪的体质量最大.结论 系统测定了雌性和雄性巴马小型猪、贵州小型猪和版纳小型猪的繁殖性能,为实验用小型猪提供了遗传育种工作的基础,也为小型猪实验动物化提供了背景数据.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.