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1.
目的 考察不同培养条件对桑黄菌液体发酵生产多糖的影响。方法 通过正交试验设计,对不同培养基种类、起始pH值、发酵温度和摇床转速进行优化,在此工艺基础上,采用5 L发酵罐进行发酵放大试验。结果 采用添加0.3 g/L维生素B1和0.3 g/L维生素B2的PD培养基,起始pH值5,发酵温度28 ℃,摇床转速180 r/min条件下,桑黄菌摇瓶发酵液中胞内多糖和胞外多糖分别为5.80、2.37 g/L;5 L发酵罐发酵液中桑黄胞内多糖和胞外多糖分别为5.85、2.30 g/L。结论 桑黄液体发酵可获得桑黄多糖,本实验结果为桑黄液体发酵工业化生产提供参考。  相似文献   

2.
以甲醇为唯一碳源,对生丝微菌TH205进行培养,研究了甲醇流加速度、Fe^2 浓度对细胞生长及吡咯喹啉醌(PQQ)表达量的影响。结果表明:培养基中添加浓度为7μg/mL的Fe^2 ,并控制甲醇的流加速度使培养基中甲醇浓度为1mL/L时,细胞的生长情况较好,且此时PQQ的发酵产量为26.6μg/mL。  相似文献   

3.
目的:探讨新生隐球菌荚膜体外形成条件,为实验教学提供一种简便、安全、成本低、效果好的实验方法。方法:采用试管培养法,用麦芽糖代替葡萄糖配制培养基并加入不同浓度的兔血清和(或)维生素B1,观察新生隐球菌在不同培养基上荚膜的形成情况。结果:新生隐球菌在含1.4mL 6%麦芽糖+0.4mL兔血清+0.2mL维生素B1的培养基中培养4天荚膜形成最佳。结论:在1.4mL6%麦芽糖+0.4mL兔血清+0.2mL维生素B1的培养基中新生隐球菌可形成典型荚膜。  相似文献   

4.
《医学教育探索》2010,33(2):121-124
目的:研究不同培养基组成成分对红缘拟层孔菌菌丝产量和多糖量的影响,优选出的红缘拟层孔菌液体发酵培养基的最佳配方。方法:以菌丝体生物量和胞内多糖的量为指标,通过单因素试验确定液体培养基中最佳碳源、氮源及维生素B1的用量。通过L9(34)正交试验确定红缘拟层孔菌的液体发酵培养基配方的最佳组合。结果:优选出的红缘拟层孔菌液体发酵培养基最佳配方为(g/100 mL):大米粉5,酵母粉1,磷酸二氢钾0.1,硫酸镁0.15,维生素B10.04。结论:优选出红缘拟层孔菌的液体发酵培养基的最佳配方,为今后大规模液体发酵研究奠定了基础。  相似文献   

5.
HPLC法测定血浆中5-氟尿嘧啶水平   总被引:1,自引:0,他引:1  
目的:建立快速测定人血浆中5-氟尿嘧啶(5-Fu)浓度的方法。方法:乙酸乙酯提取血浆中5-Fu,高压液相色谱法测定其浓度。色谱柱为YWGC18,流动相为0.03mol磷酸氢二钾(2.5:1),pH7.0,流速为1.0mL/min。紫外检测波长254nm。结果:分别在血浆低浓度(0.1μg/mL~5μg/mL)和高浓度(5μg/mL~40μg/mL)呈良好线性关系。5-Fu在5μg/mL、10μg/mL、40μg/mL、300μg/mL、500μg/mL5个浓度点日间平均回收率和RSD分别为95.2%、4.6%;90.5%、6.9%;95.9%、0.93%;107%、8.5%;106%、6.0%。结论:本方法快速、准确,适合于5-Fu临床用药浓度的观察及个体药代动力学研究。  相似文献   

6.
许颖  兰进 《医学教育探索》2006,(11):1707-1710
目的研究灵芝液体发酵产漆酶的最佳培养基和培养条件。方法以灵芝S3号菌株为试验材料,以灵芝漆酶活性为衡量指标,通过正交试验分别对灵芝液体发酵培养基及培养条件进行优化筛选。结果筛出最佳培养基组成为葡萄糖30g/L、棉花0.2%、磷酸氢二铵0.66g/L、干酪素0.5%、聚山梨酯-800.15%;优化培养条件为初始pH5.5、装液量75mL、接种量12.5%、菌龄5×24h、培养时间9×24h。结论优化培养基及培养条件后,发酵液中灵芝漆酶活性显著提高。  相似文献   

7.
[目的]筛选并优化红缘拟层孔菌的最佳母种培养基和液体发酵培养基的配方.[方法]将等量红缘拟层孔菌分别接种4种不同的固体培养基上,定期观察各组培养基上菌丝的长势和老化度.以胞内多糖和菌丝体生物量为指标,利用单因素和正交设计实验对红缘拟层孔菌的液体培养基配方进行筛选及优化.[结果]红缘拟层孔菌液体发酵培养基最佳配方为(10g/g):大米粉5,酵母粉1,磷酸二氢钾0.1,硫酸镁0.15,维生素B10.04.[结论]优选出了红缘拟层孔菌的最佳母种培养基和液体发酵的培养基,为下一步液体培养条件的探索奠定了基础.  相似文献   

8.
目的研究铁皮石斛Dendrobiumcandidum原球茎液体悬浮培养的可行性以及接种量和培养液体积对原球茎生长的影响。方法利用完全随机实验设计和正交试验设计研究不同基本培养基、接种量和培养液体积对原球茎生长的影响。结果无论鲜重还是干重,铁皮石斛原球茎在液体培养基上均极显著好于固体培养基(P<0.001)。不同基本培养基对铁皮石斛原球茎生长影响的研究表明,培养天数为30d时,对于鲜重67-V极显著好于B5(P<0.01),B5极显著好于1/2MS(P<0.01),1/2MS显著好于MS(P<0.05);对于干重67-V与B5没有显著性差异(P>0.05),B5极显著好于1/2MS(P<0.01),1/2MS极显著好于MS(P<0.01)。接种量和培养液体积对原球茎生长影响的研究表明,接种量影响最大,体积其次,互作最小,若不考虑互作,对于鲜重和干重,最佳处理为接种量6.194g/瓶 培养液体积150mL/瓶或100mL/瓶;对于干重,若考虑互作,接种量为6.194g/瓶时,应选培养液体积150mL/瓶或100mL/瓶,接种量为3.102g/瓶时,应选培养液体积150mL/瓶或100mL/瓶,接种量为1.693g/瓶时,应选150mL/瓶或50mL/瓶。结论液体悬浮培养对铁皮石斛原球茎的生长有利,获得了最佳基本培养基、接种量和培养液体积的最佳搭配方案,表明通过液体悬浮培养生产铁皮石斛原球茎具有较好的开发应用前景。  相似文献   

9.
目的:研究猴头蒲液体培养提高菌丝生物量的最佳配方.方法:通过对猴头菌液体发酵的各常用碳源与氮源单因素试验和正交试验L9(3^4),研究了葡萄糖、玉米粉、淀粉、蔗糖、酵母粉、蚕蛹粉、蛋白胨、黄豆粉对提高菌缝生物量的影响.结果:葡萄糖、玉米粉复合碳源为最适碳源,蛋白胨、黄豆粉为最适氮源,从而得到了猴头菌液体发酵培养的最佳培养基为:葡萄糖30 g/L、玉米粉30 g/L、蛋白胨4 g/L、黄豆粉15 g/L、KH2PO4 1.5 g/L、MgSO4 0.75g/L;培养条件为:温度25℃,时间为(140~144)h,在最佳培养工艺条件下,猴头菌丝体的收率为28.1g/L,比基础工艺的菌丝收率提高了一倍多,菌丝体中的多精、多肽含量分别为基础工艺的1.34倍与1.12倍.结论:通过正交试验得到的培养基配方菌丝生物量收率高,有效成分多糖、多肽含量高,工艺稳定,可作为猴头菌液体发酵的生产工艺.  相似文献   

10.
目的:探讨思密达治疗婴幼儿秋季腹泻的疗效。方法:对经临床确诊的88例秋季腹泻的患儿随机分为两组,治疗组采用口服思密达治疗,小于1岁患儿为1g/次,大于1岁的患儿为1.5g/次,疗程1d~3d;对照组40例采用口服鞣酸蛋白治疗,0.1g/次~0.2g/次,3次/d,且两组同时加复合Vit—B锌剂,1mL/次~3mL/次,3次/d。结果:治疗组总有效率为83.3%,对照组为30%,经比较有统计学意义(P〈0.01)。结论:思密达是目前治疗秋季腹泻的理想药物。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

20.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

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