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1.
高山红景天提取物对小鼠脾淋巴细胞转化反应的影响   总被引:7,自引:1,他引:6  
研究了由高山红景天中提取的红景天多糖、甙及甙元酪醇对正常小鼠脾淋巴细胞转化试验的影响.结果表明,红景天多糖在体内外均可促进小鼠淋巴细胞转化反应,且本身具有丝裂原样作用;红景天甙在体外亦可增强有丝分裂原刺激小鼠脾淋巴细胞的增殖反应,而甙元酪醇对此无影响.  相似文献   

2.
实验动物口服骨痹通天丸5.58g/kg.2.79g/kg,观察本品对炎症反应,局部疼痛和免疫功能的影响。结果表明,骨痹通天丸的两个剂量组对大鼠蛋清性关节肿具有不同程度的抑制作用(P<0.01或0.05);对二甲苯性耳廓肿仅大剂量组具有明显抑制作用(P<0.01);对小鼠热刺激所致的疼痛反应具有不同程度的抑制作用(P<0.01或0.05);对化学刺性疼痛两个剂量组均有明显抑制作用(P<0.01);并可抑制小鼠重要免疫器官的增长和巨噬细胞的吞噬功能(P<0.05),对血清溶血素未显示其效应。  相似文献   

3.
旱莲草对老龄小鼠脾淋巴细胞功能的影响   总被引:1,自引:0,他引:1  
目的探讨旱莲草(HEP)对老龄小鼠脾淋巴细胞功能的影响。方法观察老龄小鼠服旱莲草水煎剂后,脾淋巴细胞IL2、MAF的分泌和DNA合成的变化;早莲草体外对脾淋巴细胞DNA合成的影响。结果1.使老龄小鼠脾淋巴细胞分泌IL-2逐渐减少(P<0.01),并呈剂量依赖关系(r=-0.8988);2.体内外实验表明高浓度旱莲草水煎剂抑制脾淋巴细胞在ConA激活后的DNA合成,但无ConA时DNA合成不受影响;3.老龄小鼠脾淋巴细胞分泌MAF能力明显低于中龄小鼠(PMO.05),灌服旱莲草24g/kg×7d使老龄小鼠脾淋巴细胞分泌MAF能力明显增强(P<0.05)。结论HEP能明显促进老龄小鼠分泌MAF,说明HEP能提高老龄小鼠的非特异免疫功能。  相似文献   

4.
苦地丁对小鼠免疫功能影响的实验研究   总被引:4,自引:0,他引:4  
目的探讨苦地丁对小鼠免疫功能的影响。方法实验组小鼠灌胃给苦地丁水煎剂(0.2g/ml),每次0.5ml,每日1次;对照组小鼠灌胃给同等量生理盐水;7d后两组小鼠同时测定脾、胸腺重量,腹腔巨噬细胞吞噬功能,脾淋巴细胞增殖反应及IL-2活性。结果苦地丁可使脾和胸腺萎缩,巨噬细胞吞噬功能降低,淋巴细胞增殖反应受到抑制,IL-2活性减弱,与对照组比较具有非常显著性差异(P<0.01)。结论苦地丁对小鼠免疫功能有明显的抑制作用。  相似文献   

5.
香菇多糖抗肿瘤作用及其机制的研究   总被引:17,自引:0,他引:17  
目的:运用中医“药食同源”的原理,研究香菇多糖对S180荷瘤小鼠的抗肿瘤作用及其机制。方法:制备S180荷瘤小鼠动物模型,随机分为荷瘤对照组,低、中、高剂量组;通过测定荷瘤小鼠的瘤重、抑瘤率及瘤体比观察香菇多糖对肿瘤生长的影响;通过检测脾细胞的增殖分化、NK细胞的活性以及腹腔巨噬细胞的吞噬功能,研究香菇多糖对荷瘤小鼠非特异性免疫和细胞免疫功能的影响。结果:香菇多糖能明显减小S180荷瘤小鼠的瘤重和瘤体比(P〈0.05),明显抑制肿瘤的生长(P〈0.05),低、中、高剂量组的平均抑瘤率分别为37%、46%、490%;明显增强S180荷瘤小鼠腹腔巨噬细胞的吞噬功能(P〈0.05);明显提高S180荷瘤小鼠脾细胞的转化功能和NK细胞的活性(P〈0.05)。结论:香菇多糖能明显抑制S180荷瘤小鼠肿瘤的生长,其作用机制可能是通过增强荷瘤小鼠的非特异性免疫功能和细胞免疫功能。  相似文献   

6.
黄芩甙对小鼠细胞免疫功能的影响   总被引:33,自引:0,他引:33  
报告黄芩甙对细胞免疫功能的影响。结果表明.低剂量黄芩甙可明显促进Con A诱导的小鼠脾淋巴细胞增殖反应,高剂量则显著抑制。低剂量黄芩甙对LPS诱导淋巴细胞增殖反应有促进作用,但无统计学意义,高剂量可显著抑制淋巴细胞增殖。黄芩甙可明显提高小鼠脾脏单个核细胞中cAMP含量,对cGMP无影响。体内用药对巨噬细胞吞噬中性红的作用,低剂量显著升高,高剂量明显抑制。但体外用药均明显抑制巨噬细胞吞噬中性红的作用。  相似文献   

7.
用3H-TdR掺入法,测定受分次γ线照射的小鼠脾淋巴细胞转化功能,同时测定受照小鼠胞、肺、肝、脾SOD活力和LPO含量。结果表明,榄香烯提高了小鼠T、B淋巴细胞的辐射抗性(P<0.05),以及脾、肝、肺SOD活力(p<0.01、P<0.05),降低脾LPO含量(p<0.01)。提示榄香烯的辐射免疫保护机理之一,是由于增强组织SOD活力,降低LPO含量,从而减少自由基及有害物质对淋巴细胞的损伤。  相似文献   

8.
目的探讨雪灵芝对小鼠免疫功能的调节作用。方法以小鼠为实验对象,分别以0.21、0.42、0.63ml/kg·bw 3个剂量组的雪灵芝给予小鼠灌胃30-35D,进行脾淋巴细胞转化、迟发型变态反应、抗体生成细胞、血清溶血素、碳廓清、腹腔巨噬细胞吞噬鸡红细胞、NK细胞活性等七项免疫指标的测定。结果各剂量组的各项检测结果与阴性对照组比,均能刺激小鼠脾淋巴细胞增值、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,增强小鼠的单核-腹腔巨噬细胞吞噬和碳廓清能力,但对小鼠NK细胞活性功能无明显作用。结论雪灵芝具有增强小鼠免疫功能作用。  相似文献   

9.
目的 观察天山雪莲水提取物(ES)对电离辐射损伤小鼠的保护作用。方法 小鼠ig ES0.75,1.5,3.0g/kg,14d后接受8和2.5Gy的照射,观察8Gy照射小鼠的平均生存时间和生存率及2.5Gy照射小鼠的脾T淋巴细胞转化能力和骨髓DNA含量。结果 ES能显著延长8Gy照射小鼠的平均生存时间(P<0.05),并提高生存率;显著提高2.5Gy照射小鼠的脾T淋巴细胞转化能力和骨髓DNA含量(P<0.05,0.01),改善免疫功能。结论ES具有抗辐射损伤作用。  相似文献   

10.
黄芩甙对小鼠细胞免疫功能的影响   总被引:1,自引:0,他引:1  
报告黄芩甙对细胞免疫功能的影响。结果表明,低剂量黄芩甙可明显促进ConA诱导的小鼠脾淋巴细胞增殖反应,高剂量则显著抑制。低剂量黄芩甙对LPS诱导淋巴细胞增殖反应有促进作用,但无统计学意义,高剂量可显著抑制淋巴细胞增殖。黄芩甙可明显提高小鼠脾脏单个核细胞中cAMP含量,对cGMP无影响。体内用药对巨噬细胞吞噬中性红的作用,低剂量显著升高,高剂量明显抑制。但体外用药均明显抑制巨噬细胞吞噬中性红的作用。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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